Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3675 and Pf6N2E2_3676 are separated by 396 nucleotides Pf6N2E2_3676 and Pf6N2E2_3677 are separated by 182 nucleotides
Pf6N2E2_3675 - Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex (EC 2.3.1.12), at 4,280,531 to 4,282,168
_3675
Pf6N2E2_3676 - Phosphodiesterase/alkaline phosphatase D-like, at 4,282,565 to 4,283,956
_3676
Pf6N2E2_3677 - Sensory box/GGDEF family protein, at 4,284,139 to 4,286,832
_3677
Position (kb)
4282
4283
4284 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4282.167 kb on - strand at 4282.231 kb on + strand at 4282.429 kb on - strand at 4282.429 kb on - strand at 4282.441 kb on - strand at 4282.441 kb on - strand at 4282.478 kb on + strand at 4282.478 kb on + strand at 4282.478 kb on + strand at 4282.479 kb on - strand at 4282.479 kb on - strand at 4282.482 kb on + strand at 4282.500 kb on - strand at 4282.564 kb on + strand at 4282.564 kb on + strand at 4282.565 kb on - strand at 4282.576 kb on + strand at 4282.577 kb on - strand at 4282.577 kb on - strand at 4282.690 kb on + strand at 4282.877 kb on + strand, within Pf6N2E2_3676 at 4282.878 kb on - strand, within Pf6N2E2_3676 at 4282.878 kb on - strand, within Pf6N2E2_3676 at 4282.878 kb on - strand, within Pf6N2E2_3676 at 4282.885 kb on - strand, within Pf6N2E2_3676 at 4282.923 kb on - strand, within Pf6N2E2_3676 at 4282.923 kb on - strand, within Pf6N2E2_3676 at 4282.923 kb on - strand, within Pf6N2E2_3676 at 4282.923 kb on - strand, within Pf6N2E2_3676 at 4282.923 kb on - strand, within Pf6N2E2_3676 at 4282.923 kb on - strand, within Pf6N2E2_3676 at 4282.923 kb on - strand, within Pf6N2E2_3676 at 4282.923 kb on - strand, within Pf6N2E2_3676 at 4282.923 kb on - strand, within Pf6N2E2_3676 at 4282.924 kb on + strand, within Pf6N2E2_3676 at 4282.924 kb on + strand, within Pf6N2E2_3676 at 4282.924 kb on + strand, within Pf6N2E2_3676 at 4282.925 kb on - strand, within Pf6N2E2_3676 at 4282.925 kb on - strand, within Pf6N2E2_3676 at 4282.925 kb on - strand, within Pf6N2E2_3676 at 4282.925 kb on - strand, within Pf6N2E2_3676 at 4282.944 kb on - strand, within Pf6N2E2_3676 at 4282.974 kb on + strand, within Pf6N2E2_3676 at 4282.975 kb on - strand, within Pf6N2E2_3676 at 4283.072 kb on + strand, within Pf6N2E2_3676 at 4283.073 kb on - strand, within Pf6N2E2_3676 at 4283.073 kb on - strand, within Pf6N2E2_3676 at 4283.073 kb on - strand, within Pf6N2E2_3676 at 4283.073 kb on - strand, within Pf6N2E2_3676 at 4283.185 kb on + strand, within Pf6N2E2_3676 at 4283.186 kb on - strand, within Pf6N2E2_3676 at 4283.189 kb on + strand, within Pf6N2E2_3676 at 4283.189 kb on + strand, within Pf6N2E2_3676 at 4283.189 kb on + strand, within Pf6N2E2_3676 at 4283.189 kb on + strand, within Pf6N2E2_3676 at 4283.189 kb on + strand, within Pf6N2E2_3676 at 4283.189 kb on + strand, within Pf6N2E2_3676 at 4283.189 kb on + strand, within Pf6N2E2_3676 at 4283.189 kb on + strand, within Pf6N2E2_3676 at 4283.189 kb on + strand, within Pf6N2E2_3676 at 4283.190 kb on - strand, within Pf6N2E2_3676 at 4283.190 kb on - strand, within Pf6N2E2_3676 at 4283.190 kb on - strand, within Pf6N2E2_3676 at 4283.190 kb on - strand, within Pf6N2E2_3676 at 4283.190 kb on - strand, within Pf6N2E2_3676 at 4283.190 kb on - strand, within Pf6N2E2_3676 at 4283.190 kb on - strand, within Pf6N2E2_3676 at 4283.190 kb on - strand, within Pf6N2E2_3676 at 4283.261 kb on + strand, within Pf6N2E2_3676 at 4283.291 kb on + strand, within Pf6N2E2_3676 at 4283.340 kb on + strand, within Pf6N2E2_3676 at 4283.340 kb on + strand, within Pf6N2E2_3676 at 4283.341 kb on - strand, within Pf6N2E2_3676 at 4283.341 kb on - strand, within Pf6N2E2_3676 at 4283.366 kb on + strand, within Pf6N2E2_3676 at 4283.367 kb on - strand, within Pf6N2E2_3676 at 4283.367 kb on - strand, within Pf6N2E2_3676 at 4283.367 kb on - strand, within Pf6N2E2_3676 at 4283.367 kb on - strand, within Pf6N2E2_3676 at 4283.613 kb on + strand, within Pf6N2E2_3676 at 4283.614 kb on - strand, within Pf6N2E2_3676 at 4283.738 kb on + strand, within Pf6N2E2_3676 at 4283.738 kb on + strand, within Pf6N2E2_3676 at 4283.739 kb on - strand, within Pf6N2E2_3676 at 4283.739 kb on - strand, within Pf6N2E2_3676 at 4283.739 kb on - strand, within Pf6N2E2_3676 at 4283.739 kb on - strand, within Pf6N2E2_3676 at 4283.746 kb on + strand, within Pf6N2E2_3676 at 4283.747 kb on - strand, within Pf6N2E2_3676 at 4283.747 kb on - strand, within Pf6N2E2_3676 at 4283.819 kb on + strand at 4283.867 kb on + strand at 4283.868 kb on - strand at 4284.019 kb on + strand at 4284.020 kb on - strand at 4284.021 kb on + strand at 4284.085 kb on + strand at 4284.086 kb on - strand at 4284.086 kb on - strand at 4284.089 kb on - strand at 4284.194 kb on + strand at 4284.195 kb on - strand at 4284.217 kb on - strand at 4284.286 kb on + strand at 4284.306 kb on - strand at 4284.364 kb on + strand at 4284.412 kb on + strand, within Pf6N2E2_3677 at 4284.412 kb on + strand, within Pf6N2E2_3677 at 4284.412 kb on + strand, within Pf6N2E2_3677 at 4284.478 kb on + strand, within Pf6N2E2_3677 at 4284.479 kb on - strand, within Pf6N2E2_3677 at 4284.479 kb on - strand, within Pf6N2E2_3677 at 4284.479 kb on - strand, within Pf6N2E2_3677 at 4284.479 kb on - strand, within Pf6N2E2_3677 at 4284.479 kb on - strand, within Pf6N2E2_3677 at 4284.479 kb on - strand, within Pf6N2E2_3677 at 4284.479 kb on - strand, within Pf6N2E2_3677 at 4284.479 kb on - strand, within Pf6N2E2_3677 at 4284.479 kb on - strand, within Pf6N2E2_3677 at 4284.479 kb on - strand, within Pf6N2E2_3677 at 4284.479 kb on - strand, within Pf6N2E2_3677 at 4284.481 kb on - strand, within Pf6N2E2_3677 at 4284.481 kb on - strand, within Pf6N2E2_3677 at 4284.536 kb on + strand, within Pf6N2E2_3677 at 4284.537 kb on - strand, within Pf6N2E2_3677 at 4284.698 kb on - strand, within Pf6N2E2_3677 at 4284.698 kb on - strand, within Pf6N2E2_3677 at 4284.760 kb on + strand, within Pf6N2E2_3677 at 4284.760 kb on + strand, within Pf6N2E2_3677 at 4284.761 kb on - strand, within Pf6N2E2_3677 at 4284.761 kb on - strand, within Pf6N2E2_3677 at 4284.761 kb on - strand, within Pf6N2E2_3677 at 4284.761 kb on - strand, within Pf6N2E2_3677 at 4284.761 kb on - strand, within Pf6N2E2_3677 at 4284.761 kb on - strand, within Pf6N2E2_3677 at 4284.823 kb on + strand, within Pf6N2E2_3677 at 4284.844 kb on + strand, within Pf6N2E2_3677 at 4284.844 kb on + strand, within Pf6N2E2_3677 at 4284.844 kb on + strand, within Pf6N2E2_3677 at 4284.845 kb on - strand, within Pf6N2E2_3677 at 4284.886 kb on + strand, within Pf6N2E2_3677 at 4284.887 kb on - strand, within Pf6N2E2_3677
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 4,282,167 - -2.7 4,282,231 + -0.3 4,282,429 - +0.0 4,282,429 - -1.1 4,282,441 - -0.1 4,282,441 - +0.8 4,282,478 + -0.6 4,282,478 + +0.3 4,282,478 + -0.8 4,282,479 - +0.0 4,282,479 - -0.3 4,282,482 + +0.3 4,282,500 - +0.2 4,282,564 + +0.1 4,282,564 + +0.0 4,282,565 - +1.0 4,282,576 + -0.2 4,282,577 - +1.0 4,282,577 - +0.7 4,282,690 + +0.5 4,282,877 + Pf6N2E2_3676 0.22 +0.2 4,282,878 - Pf6N2E2_3676 0.22 +0.1 4,282,878 - Pf6N2E2_3676 0.22 +0.6 4,282,878 - Pf6N2E2_3676 0.22 +0.3 4,282,885 - Pf6N2E2_3676 0.23 +1.0 4,282,923 - Pf6N2E2_3676 0.26 +0.1 4,282,923 - Pf6N2E2_3676 0.26 +0.4 4,282,923 - Pf6N2E2_3676 0.26 -0.3 4,282,923 - Pf6N2E2_3676 0.26 +0.5 4,282,923 - Pf6N2E2_3676 0.26 -0.5 4,282,923 - Pf6N2E2_3676 0.26 +0.0 4,282,923 - Pf6N2E2_3676 0.26 +0.4 4,282,923 - Pf6N2E2_3676 0.26 -0.2 4,282,923 - Pf6N2E2_3676 0.26 +0.1 4,282,924 + Pf6N2E2_3676 0.26 -0.1 4,282,924 + Pf6N2E2_3676 0.26 -0.7 4,282,924 + Pf6N2E2_3676 0.26 -1.3 4,282,925 - Pf6N2E2_3676 0.26 +1.0 4,282,925 - Pf6N2E2_3676 0.26 +0.4 4,282,925 - Pf6N2E2_3676 0.26 +0.5 4,282,925 - Pf6N2E2_3676 0.26 +0.3 4,282,944 - Pf6N2E2_3676 0.27 +0.8 4,282,974 + Pf6N2E2_3676 0.29 +1.1 4,282,975 - Pf6N2E2_3676 0.29 -0.5 4,283,072 + Pf6N2E2_3676 0.36 -0.7 4,283,073 - Pf6N2E2_3676 0.36 -0.4 4,283,073 - Pf6N2E2_3676 0.36 -1.5 4,283,073 - Pf6N2E2_3676 0.36 +1.1 4,283,073 - Pf6N2E2_3676 0.36 +0.1 4,283,185 + Pf6N2E2_3676 0.45 +1.5 4,283,186 - Pf6N2E2_3676 0.45 -0.4 4,283,189 + Pf6N2E2_3676 0.45 +0.6 4,283,189 + Pf6N2E2_3676 0.45 +0.7 4,283,189 + Pf6N2E2_3676 0.45 -1.5 4,283,189 + Pf6N2E2_3676 0.45 +0.1 4,283,189 + Pf6N2E2_3676 0.45 -1.0 4,283,189 + Pf6N2E2_3676 0.45 +0.5 4,283,189 + Pf6N2E2_3676 0.45 +0.3 4,283,189 + Pf6N2E2_3676 0.45 +1.5 4,283,189 + Pf6N2E2_3676 0.45 +1.5 4,283,190 - Pf6N2E2_3676 0.45 -0.4 4,283,190 - Pf6N2E2_3676 0.45 -1.7 4,283,190 - Pf6N2E2_3676 0.45 +0.2 4,283,190 - Pf6N2E2_3676 0.45 -0.0 4,283,190 - Pf6N2E2_3676 0.45 -0.3 4,283,190 - Pf6N2E2_3676 0.45 +0.1 4,283,190 - Pf6N2E2_3676 0.45 -0.0 4,283,190 - Pf6N2E2_3676 0.45 -0.6 4,283,261 + Pf6N2E2_3676 0.50 -0.0 4,283,291 + Pf6N2E2_3676 0.52 -1.3 4,283,340 + Pf6N2E2_3676 0.56 -1.1 4,283,340 + Pf6N2E2_3676 0.56 -1.6 4,283,341 - Pf6N2E2_3676 0.56 -0.0 4,283,341 - Pf6N2E2_3676 0.56 -0.7 4,283,366 + Pf6N2E2_3676 0.58 -0.2 4,283,367 - Pf6N2E2_3676 0.58 -0.0 4,283,367 - Pf6N2E2_3676 0.58 +0.0 4,283,367 - Pf6N2E2_3676 0.58 +0.5 4,283,367 - Pf6N2E2_3676 0.58 +0.1 4,283,613 + Pf6N2E2_3676 0.75 -0.1 4,283,614 - Pf6N2E2_3676 0.75 +0.1 4,283,738 + Pf6N2E2_3676 0.84 -0.1 4,283,738 + Pf6N2E2_3676 0.84 -0.4 4,283,739 - Pf6N2E2_3676 0.84 +0.9 4,283,739 - Pf6N2E2_3676 0.84 +0.7 4,283,739 - Pf6N2E2_3676 0.84 +0.2 4,283,739 - Pf6N2E2_3676 0.84 +0.7 4,283,746 + Pf6N2E2_3676 0.85 -0.5 4,283,747 - Pf6N2E2_3676 0.85 +0.6 4,283,747 - Pf6N2E2_3676 0.85 +0.9 4,283,819 + +0.3 4,283,867 + -0.1 4,283,868 - -0.6 4,284,019 + +0.4 4,284,020 - +1.2 4,284,021 + +1.8 4,284,085 + +1.1 4,284,086 - +0.1 4,284,086 - -0.0 4,284,089 - -0.7 4,284,194 + +0.1 4,284,195 - -2.3 4,284,217 - -0.5 4,284,286 + -1.1 4,284,306 - -1.6 4,284,364 + -2.0 4,284,412 + Pf6N2E2_3677 0.10 +0.1 4,284,412 + Pf6N2E2_3677 0.10 -0.8 4,284,412 + Pf6N2E2_3677 0.10 -0.9 4,284,478 + Pf6N2E2_3677 0.13 -1.0 4,284,479 - Pf6N2E2_3677 0.13 -2.5 4,284,479 - Pf6N2E2_3677 0.13 -0.7 4,284,479 - Pf6N2E2_3677 0.13 -2.5 4,284,479 - Pf6N2E2_3677 0.13 -2.3 4,284,479 - Pf6N2E2_3677 0.13 -2.6 4,284,479 - Pf6N2E2_3677 0.13 -0.8 4,284,479 - Pf6N2E2_3677 0.13 -0.6 4,284,479 - Pf6N2E2_3677 0.13 -1.5 4,284,479 - Pf6N2E2_3677 0.13 -1.1 4,284,479 - Pf6N2E2_3677 0.13 -0.5 4,284,479 - Pf6N2E2_3677 0.13 -1.5 4,284,481 - Pf6N2E2_3677 0.13 -3.9 4,284,481 - Pf6N2E2_3677 0.13 -1.3 4,284,536 + Pf6N2E2_3677 0.15 -0.6 4,284,537 - Pf6N2E2_3677 0.15 -0.9 4,284,698 - Pf6N2E2_3677 0.21 -1.8 4,284,698 - Pf6N2E2_3677 0.21 -1.1 4,284,760 + Pf6N2E2_3677 0.23 -1.4 4,284,760 + Pf6N2E2_3677 0.23 -1.2 4,284,761 - Pf6N2E2_3677 0.23 -0.9 4,284,761 - Pf6N2E2_3677 0.23 -1.3 4,284,761 - Pf6N2E2_3677 0.23 -1.3 4,284,761 - Pf6N2E2_3677 0.23 -1.7 4,284,761 - Pf6N2E2_3677 0.23 -1.3 4,284,761 - Pf6N2E2_3677 0.23 -2.7 4,284,823 + Pf6N2E2_3677 0.25 -1.3 4,284,844 + Pf6N2E2_3677 0.26 -1.9 4,284,844 + Pf6N2E2_3677 0.26 -0.8 4,284,844 + Pf6N2E2_3677 0.26 -1.3 4,284,845 - Pf6N2E2_3677 0.26 +0.2 4,284,886 + Pf6N2E2_3677 0.28 -0.9 4,284,887 - Pf6N2E2_3677 0.28 +0.7
Or see this region's nucleotide sequence