Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_186 and Pf6N2E2_187 are separated by 10 nucleotides Pf6N2E2_187 and Pf6N2E2_188 are separated by 132 nucleotides Pf6N2E2_188 and Pf6N2E2_189 are separated by 150 nucleotides Pf6N2E2_189 and Pf6N2E2_190 are separated by 14 nucleotides
Pf6N2E2_186 - Urea ABC transporter, ATPase protein UrtD, at 239,149 to 239,907
_186
Pf6N2E2_187 - Urea ABC transporter, ATPase protein UrtE, at 239,918 to 240,607
_187
Pf6N2E2_188 - Formamidase (EC 3.5.1.49), at 240,740 to 241,969
_188
Pf6N2E2_189 - regulatory protein, putative, at 242,120 to 242,353
_189
Pf6N2E2_190 - Aliphatic amidase AmiE (EC 3.5.1.4), at 242,368 to 243,411
_190
Position (kb)
240
241
242 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 239.808 kb on + strand, within Pf6N2E2_186 at 239.893 kb on + strand at 239.893 kb on + strand at 239.894 kb on - strand at 239.897 kb on + strand at 239.928 kb on + strand at 239.928 kb on + strand at 239.929 kb on - strand at 240.155 kb on + strand, within Pf6N2E2_187 at 240.155 kb on + strand, within Pf6N2E2_187 at 240.156 kb on - strand, within Pf6N2E2_187 at 240.156 kb on - strand, within Pf6N2E2_187 at 240.185 kb on - strand, within Pf6N2E2_187 at 240.207 kb on + strand, within Pf6N2E2_187 at 240.207 kb on + strand, within Pf6N2E2_187 at 240.207 kb on + strand, within Pf6N2E2_187 at 240.257 kb on + strand, within Pf6N2E2_187 at 240.606 kb on - strand at 240.626 kb on + strand at 240.626 kb on + strand at 240.626 kb on + strand at 240.627 kb on - strand at 240.627 kb on - strand at 240.627 kb on - strand at 240.627 kb on - strand at 240.636 kb on + strand at 240.654 kb on + strand at 240.654 kb on + strand at 240.669 kb on - strand at 240.712 kb on + strand at 240.712 kb on + strand at 240.859 kb on + strand at 240.859 kb on + strand at 240.873 kb on - strand, within Pf6N2E2_188 at 240.873 kb on - strand, within Pf6N2E2_188 at 240.873 kb on - strand, within Pf6N2E2_188 at 240.950 kb on + strand, within Pf6N2E2_188 at 240.950 kb on + strand, within Pf6N2E2_188 at 240.951 kb on - strand, within Pf6N2E2_188 at 241.405 kb on + strand, within Pf6N2E2_188 at 241.406 kb on - strand, within Pf6N2E2_188 at 241.457 kb on + strand, within Pf6N2E2_188 at 241.558 kb on + strand, within Pf6N2E2_188 at 241.644 kb on - strand, within Pf6N2E2_188 at 241.652 kb on + strand, within Pf6N2E2_188 at 241.653 kb on - strand, within Pf6N2E2_188 at 241.653 kb on - strand, within Pf6N2E2_188 at 241.653 kb on - strand, within Pf6N2E2_188 at 241.669 kb on + strand, within Pf6N2E2_188 at 241.752 kb on - strand, within Pf6N2E2_188 at 241.752 kb on - strand, within Pf6N2E2_188 at 241.788 kb on - strand, within Pf6N2E2_188 at 241.822 kb on + strand, within Pf6N2E2_188 at 242.015 kb on + strand at 242.015 kb on + strand at 242.015 kb on + strand at 242.016 kb on - strand at 242.021 kb on + strand at 242.022 kb on - strand at 242.222 kb on + strand, within Pf6N2E2_189 at 242.222 kb on + strand, within Pf6N2E2_189 at 242.318 kb on - strand, within Pf6N2E2_189 at 242.318 kb on - strand, within Pf6N2E2_189 at 242.351 kb on + strand at 242.360 kb on + strand at 242.360 kb on + strand at 242.360 kb on + strand at 242.360 kb on + strand at 242.383 kb on - strand at 242.425 kb on + strand at 242.445 kb on + strand at 242.445 kb on + strand at 242.445 kb on + strand at 242.445 kb on + strand at 242.446 kb on - strand at 242.545 kb on + strand, within Pf6N2E2_190 at 242.566 kb on + strand, within Pf6N2E2_190 at 242.566 kb on + strand, within Pf6N2E2_190 at 242.567 kb on - strand, within Pf6N2E2_190 at 242.567 kb on - strand, within Pf6N2E2_190 at 242.601 kb on + strand, within Pf6N2E2_190 at 242.713 kb on + strand, within Pf6N2E2_190 at 242.714 kb on - strand, within Pf6N2E2_190 at 242.789 kb on + strand, within Pf6N2E2_190 at 242.790 kb on - strand, within Pf6N2E2_190 at 242.790 kb on - strand, within Pf6N2E2_190 at 242.790 kb on - strand, within Pf6N2E2_190 at 242.818 kb on + strand, within Pf6N2E2_190 at 242.819 kb on - strand, within Pf6N2E2_190 at 242.819 kb on - strand, within Pf6N2E2_190 at 242.879 kb on - strand, within Pf6N2E2_190 at 242.941 kb on + strand, within Pf6N2E2_190 at 242.941 kb on + strand, within Pf6N2E2_190 at 242.941 kb on + strand, within Pf6N2E2_190 at 242.941 kb on + strand, within Pf6N2E2_190 at 242.941 kb on + strand, within Pf6N2E2_190 at 242.942 kb on - strand, within Pf6N2E2_190 at 242.942 kb on - strand, within Pf6N2E2_190 at 242.942 kb on - strand, within Pf6N2E2_190 at 242.943 kb on + strand, within Pf6N2E2_190 at 242.943 kb on + strand, within Pf6N2E2_190 at 242.943 kb on + strand, within Pf6N2E2_190 at 242.943 kb on + strand, within Pf6N2E2_190 at 242.943 kb on + strand, within Pf6N2E2_190 at 242.943 kb on + strand, within Pf6N2E2_190 at 242.943 kb on + strand, within Pf6N2E2_190 at 242.943 kb on + strand, within Pf6N2E2_190 at 242.943 kb on + strand, within Pf6N2E2_190 at 242.943 kb on + strand, within Pf6N2E2_190 at 242.943 kb on + strand, within Pf6N2E2_190 at 242.943 kb on + strand, within Pf6N2E2_190 at 242.943 kb on + strand, within Pf6N2E2_190 at 242.943 kb on + strand, within Pf6N2E2_190 at 242.944 kb on - strand, within Pf6N2E2_190 at 242.944 kb on - strand, within Pf6N2E2_190 at 242.944 kb on - strand, within Pf6N2E2_190 at 242.944 kb on - strand, within Pf6N2E2_190 at 242.944 kb on - strand, within Pf6N2E2_190 at 242.944 kb on - strand, within Pf6N2E2_190 at 242.944 kb on - strand, within Pf6N2E2_190 at 242.944 kb on - strand, within Pf6N2E2_190 at 242.944 kb on - strand, within Pf6N2E2_190 at 242.944 kb on - strand, within Pf6N2E2_190 at 242.944 kb on - strand, within Pf6N2E2_190 at 242.944 kb on - strand, within Pf6N2E2_190 at 242.944 kb on - strand, within Pf6N2E2_190
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 239,808 + Pf6N2E2_186 0.87 -1.3 239,893 + -1.8 239,893 + -0.2 239,894 - -1.6 239,897 + +0.4 239,928 + +0.1 239,928 + +0.7 239,929 - +1.2 240,155 + Pf6N2E2_187 0.34 -0.8 240,155 + Pf6N2E2_187 0.34 +0.5 240,156 - Pf6N2E2_187 0.34 -0.2 240,156 - Pf6N2E2_187 0.34 -0.3 240,185 - Pf6N2E2_187 0.39 +0.0 240,207 + Pf6N2E2_187 0.42 -0.0 240,207 + Pf6N2E2_187 0.42 +0.4 240,207 + Pf6N2E2_187 0.42 -1.0 240,257 + Pf6N2E2_187 0.49 +0.5 240,606 - +0.9 240,626 + +0.2 240,626 + +0.5 240,626 + +0.8 240,627 - -0.2 240,627 - -0.5 240,627 - -0.8 240,627 - -0.2 240,636 + -0.7 240,654 + -0.6 240,654 + -0.7 240,669 - -0.4 240,712 + +1.4 240,712 + +0.2 240,859 + -0.5 240,859 + -0.2 240,873 - Pf6N2E2_188 0.11 -1.7 240,873 - Pf6N2E2_188 0.11 -0.6 240,873 - Pf6N2E2_188 0.11 +0.3 240,950 + Pf6N2E2_188 0.17 -0.6 240,950 + Pf6N2E2_188 0.17 +0.4 240,951 - Pf6N2E2_188 0.17 +0.6 241,405 + Pf6N2E2_188 0.54 +0.8 241,406 - Pf6N2E2_188 0.54 -0.1 241,457 + Pf6N2E2_188 0.58 +1.3 241,558 + Pf6N2E2_188 0.67 -1.8 241,644 - Pf6N2E2_188 0.73 -0.3 241,652 + Pf6N2E2_188 0.74 -0.9 241,653 - Pf6N2E2_188 0.74 +0.6 241,653 - Pf6N2E2_188 0.74 -0.2 241,653 - Pf6N2E2_188 0.74 -0.2 241,669 + Pf6N2E2_188 0.76 -2.0 241,752 - Pf6N2E2_188 0.82 +0.5 241,752 - Pf6N2E2_188 0.82 +0.0 241,788 - Pf6N2E2_188 0.85 -2.5 241,822 + Pf6N2E2_188 0.88 -0.2 242,015 + +0.6 242,015 + -1.4 242,015 + -0.4 242,016 - -0.4 242,021 + -0.7 242,022 - -1.8 242,222 + Pf6N2E2_189 0.44 -0.0 242,222 + Pf6N2E2_189 0.44 +0.2 242,318 - Pf6N2E2_189 0.85 +1.3 242,318 - Pf6N2E2_189 0.85 -1.2 242,351 + +1.0 242,360 + -0.5 242,360 + +0.4 242,360 + +0.5 242,360 + -0.5 242,383 - +0.8 242,425 + -2.5 242,445 + +0.8 242,445 + +1.4 242,445 + -0.5 242,445 + -1.5 242,446 - +0.2 242,545 + Pf6N2E2_190 0.17 -0.6 242,566 + Pf6N2E2_190 0.19 -0.3 242,566 + Pf6N2E2_190 0.19 +1.1 242,567 - Pf6N2E2_190 0.19 +0.6 242,567 - Pf6N2E2_190 0.19 +1.7 242,601 + Pf6N2E2_190 0.22 -0.2 242,713 + Pf6N2E2_190 0.33 +1.2 242,714 - Pf6N2E2_190 0.33 +1.2 242,789 + Pf6N2E2_190 0.40 -0.3 242,790 - Pf6N2E2_190 0.40 +0.4 242,790 - Pf6N2E2_190 0.40 +1.4 242,790 - Pf6N2E2_190 0.40 +1.0 242,818 + Pf6N2E2_190 0.43 +1.9 242,819 - Pf6N2E2_190 0.43 +0.4 242,819 - Pf6N2E2_190 0.43 +0.0 242,879 - Pf6N2E2_190 0.49 +0.7 242,941 + Pf6N2E2_190 0.55 +0.8 242,941 + Pf6N2E2_190 0.55 -0.6 242,941 + Pf6N2E2_190 0.55 -0.7 242,941 + Pf6N2E2_190 0.55 -0.9 242,941 + Pf6N2E2_190 0.55 -0.1 242,942 - Pf6N2E2_190 0.55 +1.8 242,942 - Pf6N2E2_190 0.55 -2.9 242,942 - Pf6N2E2_190 0.55 -0.9 242,943 + Pf6N2E2_190 0.55 -0.3 242,943 + Pf6N2E2_190 0.55 -1.1 242,943 + Pf6N2E2_190 0.55 +1.3 242,943 + Pf6N2E2_190 0.55 -1.2 242,943 + Pf6N2E2_190 0.55 +0.1 242,943 + Pf6N2E2_190 0.55 -0.3 242,943 + Pf6N2E2_190 0.55 +0.9 242,943 + Pf6N2E2_190 0.55 +2.1 242,943 + Pf6N2E2_190 0.55 -0.2 242,943 + Pf6N2E2_190 0.55 -0.9 242,943 + Pf6N2E2_190 0.55 -1.0 242,943 + Pf6N2E2_190 0.55 -0.4 242,943 + Pf6N2E2_190 0.55 +0.4 242,943 + Pf6N2E2_190 0.55 -0.2 242,944 - Pf6N2E2_190 0.55 +0.8 242,944 - Pf6N2E2_190 0.55 +0.1 242,944 - Pf6N2E2_190 0.55 -0.2 242,944 - Pf6N2E2_190 0.55 +0.6 242,944 - Pf6N2E2_190 0.55 -0.1 242,944 - Pf6N2E2_190 0.55 +1.2 242,944 - Pf6N2E2_190 0.55 +0.2 242,944 - Pf6N2E2_190 0.55 +0.8 242,944 - Pf6N2E2_190 0.55 +1.4 242,944 - Pf6N2E2_190 0.55 -0.0 242,944 - Pf6N2E2_190 0.55 +0.5 242,944 - Pf6N2E2_190 0.55 +1.1 242,944 - Pf6N2E2_190 0.55 +0.8
Or see this region's nucleotide sequence