Strain Fitness in Methanococcus maripaludis S2 around MMP_RS05040

Experiment: Mc formate, casamino free, with 10mM L-Ornithine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS05030 and MMP_RS05035 are separated by 49 nucleotidesMMP_RS05035 and MMP_RS05040 are separated by 77 nucleotidesMMP_RS05040 and MMP_RS05045 overlap by 14 nucleotidesMMP_RS05045 and MMP_RS05050 are separated by 51 nucleotides MMP_RS05030: MMP_RS05030 - adenylosuccinate lyase, at 958,096 to 959,445 _RS05030 MMP_RS05035: MMP_RS05035 - hypothetical protein, at 959,495 to 959,908 _RS05035 MMP_RS05040: MMP_RS05040 - permease, at 959,986 to 960,843 _RS05040 MMP_RS05045: MMP_RS05045 - 4Fe-4S binding protein, at 960,830 to 961,489 _RS05045 MMP_RS05050: MMP_RS05050 - substrate-binding domain-containing protein, at 961,541 to 962,392 _RS05050 Position (kb) 959 960 961Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 959.103 kb on + strandat 959.206 kb on + strandat 959.206 kb on + strandat 959.206 kb on + strandat 959.338 kb on - strandat 959.339 kb on + strandat 959.384 kb on + strandat 959.443 kb on - strandat 959.463 kb on + strandat 959.469 kb on - strandat 959.469 kb on - strandat 959.469 kb on - strandat 959.470 kb on + strandat 959.470 kb on + strandat 959.507 kb on + strandat 959.508 kb on - strandat 959.513 kb on - strandat 959.513 kb on - strandat 959.569 kb on + strand, within MMP_RS05035at 959.574 kb on + strand, within MMP_RS05035at 959.591 kb on + strand, within MMP_RS05035at 959.663 kb on + strand, within MMP_RS05035at 959.666 kb on - strand, within MMP_RS05035at 959.734 kb on - strand, within MMP_RS05035at 959.738 kb on - strand, within MMP_RS05035at 959.739 kb on + strand, within MMP_RS05035at 959.739 kb on + strand, within MMP_RS05035at 959.911 kb on - strandat 959.925 kb on + strandat 959.935 kb on - strandat 959.939 kb on + strandat 959.952 kb on - strandat 959.952 kb on - strandat 959.952 kb on - strandat 959.952 kb on - strandat 959.953 kb on + strandat 959.953 kb on + strandat 959.953 kb on + strandat 959.953 kb on + strandat 959.953 kb on + strandat 959.954 kb on - strandat 959.956 kb on - strandat 959.956 kb on - strandat 959.957 kb on + strandat 959.957 kb on + strandat 959.997 kb on - strandat 959.997 kb on - strandat 959.998 kb on + strandat 959.998 kb on + strandat 959.998 kb on + strandat 960.030 kb on - strandat 960.030 kb on - strandat 960.035 kb on + strandat 960.048 kb on + strandat 960.107 kb on + strand, within MMP_RS05040at 960.107 kb on + strand, within MMP_RS05040at 960.139 kb on + strand, within MMP_RS05040at 960.174 kb on - strand, within MMP_RS05040at 960.175 kb on + strand, within MMP_RS05040at 960.175 kb on + strand, within MMP_RS05040at 960.175 kb on + strand, within MMP_RS05040at 960.208 kb on - strand, within MMP_RS05040at 960.208 kb on - strand, within MMP_RS05040at 960.209 kb on + strand, within MMP_RS05040at 960.210 kb on - strand, within MMP_RS05040at 960.210 kb on - strand, within MMP_RS05040at 960.239 kb on - strand, within MMP_RS05040at 960.246 kb on - strand, within MMP_RS05040at 960.246 kb on - strand, within MMP_RS05040at 960.267 kb on + strand, within MMP_RS05040at 960.294 kb on + strand, within MMP_RS05040at 960.473 kb on + strand, within MMP_RS05040at 960.512 kb on - strand, within MMP_RS05040at 960.512 kb on - strand, within MMP_RS05040at 960.557 kb on + strand, within MMP_RS05040at 960.558 kb on - strand, within MMP_RS05040at 960.571 kb on + strand, within MMP_RS05040at 960.571 kb on + strand, within MMP_RS05040at 960.667 kb on + strand, within MMP_RS05040at 960.667 kb on + strand, within MMP_RS05040at 960.697 kb on + strand, within MMP_RS05040at 960.743 kb on - strand, within MMP_RS05040at 960.743 kb on - strand, within MMP_RS05040at 960.744 kb on + strand, within MMP_RS05040at 960.769 kb on - strandat 960.773 kb on - strandat 960.774 kb on + strandat 960.819 kb on - strandat 960.931 kb on + strand, within MMP_RS05045at 960.934 kb on - strand, within MMP_RS05045at 960.935 kb on + strand, within MMP_RS05045at 960.935 kb on + strand, within MMP_RS05045at 960.935 kb on + strand, within MMP_RS05045at 960.956 kb on - strand, within MMP_RS05045at 960.963 kb on + strand, within MMP_RS05045at 960.986 kb on - strand, within MMP_RS05045at 960.986 kb on - strand, within MMP_RS05045at 960.986 kb on - strand, within MMP_RS05045at 960.986 kb on - strand, within MMP_RS05045at 960.986 kb on - strand, within MMP_RS05045at 960.987 kb on + strand, within MMP_RS05045at 960.987 kb on + strand, within MMP_RS05045at 960.987 kb on + strand, within MMP_RS05045at 960.987 kb on + strand, within MMP_RS05045at 960.987 kb on + strand, within MMP_RS05045at 960.987 kb on + strand, within MMP_RS05045at 960.987 kb on + strand, within MMP_RS05045at 960.987 kb on + strand, within MMP_RS05045at 960.987 kb on + strand, within MMP_RS05045at 960.989 kb on + strand, within MMP_RS05045at 960.989 kb on + strand, within MMP_RS05045at 960.999 kb on - strand, within MMP_RS05045at 961.002 kb on - strand, within MMP_RS05045at 961.003 kb on + strand, within MMP_RS05045at 961.146 kb on + strand, within MMP_RS05045at 961.181 kb on + strand, within MMP_RS05045at 961.185 kb on + strand, within MMP_RS05045at 961.203 kb on - strand, within MMP_RS05045at 961.217 kb on - strand, within MMP_RS05045at 961.262 kb on + strand, within MMP_RS05045at 961.325 kb on - strand, within MMP_RS05045at 961.326 kb on + strand, within MMP_RS05045at 961.365 kb on - strand, within MMP_RS05045at 961.381 kb on - strand, within MMP_RS05045at 961.382 kb on + strand, within MMP_RS05045at 961.382 kb on + strand, within MMP_RS05045at 961.382 kb on + strand, within MMP_RS05045at 961.386 kb on + strand, within MMP_RS05045at 961.392 kb on + strand, within MMP_RS05045at 961.460 kb on + strandat 961.465 kb on - strandat 961.508 kb on - strandat 961.542 kb on - strandat 961.543 kb on + strandat 961.543 kb on + strandat 961.543 kb on + strandat 961.550 kb on - strandat 961.573 kb on + strandat 961.573 kb on + strandat 961.573 kb on + strandat 961.581 kb on - strandat 961.582 kb on + strandat 961.584 kb on + strandat 961.607 kb on - strandat 961.636 kb on - strand, within MMP_RS05050at 961.637 kb on + strand, within MMP_RS05050at 961.677 kb on - strand, within MMP_RS05050at 961.699 kb on - strand, within MMP_RS05050at 961.702 kb on + strand, within MMP_RS05050at 961.721 kb on + strand, within MMP_RS05050at 961.724 kb on - strand, within MMP_RS05050at 961.724 kb on - strand, within MMP_RS05050at 961.725 kb on + strand, within MMP_RS05050at 961.725 kb on + strand, within MMP_RS05050at 961.725 kb on + strand, within MMP_RS05050at 961.731 kb on + strand, within MMP_RS05050at 961.766 kb on + strand, within MMP_RS05050at 961.787 kb on + strand, within MMP_RS05050

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Per-strain Table

Position Strand Gene LocusTag Fraction Mc formate, casamino free, with 10mM L-Ornithine
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959,103 + -2.3
959,206 + -3.0
959,206 + -1.8
959,206 + -0.6
959,338 - -1.3
959,339 + -1.8
959,384 + -1.8
959,443 - +0.7
959,463 + -0.5
959,469 - +1.2
959,469 - +0.6
959,469 - -0.4
959,470 + +0.5
959,470 + +1.2
959,507 + -0.0
959,508 - -0.2
959,513 - +0.5
959,513 - -0.2
959,569 + MMP_RS05035 0.18 +0.5
959,574 + MMP_RS05035 0.19 -0.6
959,591 + MMP_RS05035 0.23 +0.1
959,663 + MMP_RS05035 0.41 +0.5
959,666 - MMP_RS05035 0.41 -3.6
959,734 - MMP_RS05035 0.58 +0.3
959,738 - MMP_RS05035 0.59 -0.8
959,739 + MMP_RS05035 0.59 -0.1
959,739 + MMP_RS05035 0.59 +1.0
959,911 - +0.9
959,925 + +0.2
959,935 - +0.6
959,939 + -0.7
959,952 - +0.7
959,952 - +0.7
959,952 - -0.4
959,952 - -0.3
959,953 + -0.9
959,953 + -0.2
959,953 + +1.2
959,953 + +0.7
959,953 + -0.9
959,954 - +0.7
959,956 - +0.9
959,956 - +0.1
959,957 + +0.4
959,957 + -3.8
959,997 - +0.7
959,997 - +0.7
959,998 + +0.2
959,998 + +0.7
959,998 + -0.4
960,030 - +0.4
960,030 - +0.4
960,035 + +0.9
960,048 + -0.7
960,107 + MMP_RS05040 0.14 +0.5
960,107 + MMP_RS05040 0.14 -0.5
960,139 + MMP_RS05040 0.18 +0.3
960,174 - MMP_RS05040 0.22 -0.5
960,175 + MMP_RS05040 0.22 +1.1
960,175 + MMP_RS05040 0.22 +1.0
960,175 + MMP_RS05040 0.22 -0.6
960,208 - MMP_RS05040 0.26 -0.5
960,208 - MMP_RS05040 0.26 +1.0
960,209 + MMP_RS05040 0.26 -0.2
960,210 - MMP_RS05040 0.26 +0.9
960,210 - MMP_RS05040 0.26 -1.3
960,239 - MMP_RS05040 0.29 -0.8
960,246 - MMP_RS05040 0.30 -1.5
960,246 - MMP_RS05040 0.30 +0.3
960,267 + MMP_RS05040 0.33 -1.0
960,294 + MMP_RS05040 0.36 +0.3
960,473 + MMP_RS05040 0.57 -0.8
960,512 - MMP_RS05040 0.61 -1.7
960,512 - MMP_RS05040 0.61 -0.6
960,557 + MMP_RS05040 0.67 +0.9
960,558 - MMP_RS05040 0.67 -0.6
960,571 + MMP_RS05040 0.68 +0.2
960,571 + MMP_RS05040 0.68 -3.2
960,667 + MMP_RS05040 0.79 -0.5
960,667 + MMP_RS05040 0.79 +0.4
960,697 + MMP_RS05040 0.83 -0.2
960,743 - MMP_RS05040 0.88 -3.0
960,743 - MMP_RS05040 0.88 -1.2
960,744 + MMP_RS05040 0.88 -0.1
960,769 - -0.8
960,773 - -1.2
960,774 + +0.9
960,819 - -1.0
960,931 + MMP_RS05045 0.15 -2.2
960,934 - MMP_RS05045 0.16 -3.8
960,935 + MMP_RS05045 0.16 +0.4
960,935 + MMP_RS05045 0.16 +0.3
960,935 + MMP_RS05045 0.16 +0.3
960,956 - MMP_RS05045 0.19 +0.8
960,963 + MMP_RS05045 0.20 +0.0
960,986 - MMP_RS05045 0.24 +1.2
960,986 - MMP_RS05045 0.24 +0.4
960,986 - MMP_RS05045 0.24 +0.1
960,986 - MMP_RS05045 0.24 -0.7
960,986 - MMP_RS05045 0.24 -3.2
960,987 + MMP_RS05045 0.24 +0.5
960,987 + MMP_RS05045 0.24 +0.6
960,987 + MMP_RS05045 0.24 +1.2
960,987 + MMP_RS05045 0.24 -0.7
960,987 + MMP_RS05045 0.24 +0.9
960,987 + MMP_RS05045 0.24 +0.9
960,987 + MMP_RS05045 0.24 -0.3
960,987 + MMP_RS05045 0.24 +0.4
960,987 + MMP_RS05045 0.24 -0.4
960,989 + MMP_RS05045 0.24 +0.9
960,989 + MMP_RS05045 0.24 +0.7
960,999 - MMP_RS05045 0.26 -0.6
961,002 - MMP_RS05045 0.26 +0.2
961,003 + MMP_RS05045 0.26 -0.2
961,146 + MMP_RS05045 0.48 +1.0
961,181 + MMP_RS05045 0.53 +0.8
961,185 + MMP_RS05045 0.54 -0.6
961,203 - MMP_RS05045 0.57 -1.2
961,217 - MMP_RS05045 0.59 +0.4
961,262 + MMP_RS05045 0.65 +0.4
961,325 - MMP_RS05045 0.75 +0.5
961,326 + MMP_RS05045 0.75 +0.4
961,365 - MMP_RS05045 0.81 -0.1
961,381 - MMP_RS05045 0.83 -0.2
961,382 + MMP_RS05045 0.84 -4.2
961,382 + MMP_RS05045 0.84 +0.4
961,382 + MMP_RS05045 0.84 +0.8
961,386 + MMP_RS05045 0.84 -0.2
961,392 + MMP_RS05045 0.85 +1.1
961,460 + -0.3
961,465 - -0.2
961,508 - -0.8
961,542 - -0.0
961,543 + +0.2
961,543 + +1.0
961,543 + +1.0
961,550 - +0.8
961,573 + -2.8
961,573 + -0.4
961,573 + +0.6
961,581 - +0.1
961,582 + -3.2
961,584 + +0.2
961,607 - -0.7
961,636 - MMP_RS05050 0.11 +0.2
961,637 + MMP_RS05050 0.11 -3.1
961,677 - MMP_RS05050 0.16 +0.3
961,699 - MMP_RS05050 0.19 -0.1
961,702 + MMP_RS05050 0.19 +0.3
961,721 + MMP_RS05050 0.21 +0.3
961,724 - MMP_RS05050 0.21 -0.1
961,724 - MMP_RS05050 0.21 +0.7
961,725 + MMP_RS05050 0.22 +0.8
961,725 + MMP_RS05050 0.22 +0.6
961,725 + MMP_RS05050 0.22 -1.1
961,731 + MMP_RS05050 0.22 -0.4
961,766 + MMP_RS05050 0.26 +0.9
961,787 + MMP_RS05050 0.29 +0.5

Or see this region's nucleotide sequence