Experiment: Dilute LB (1/2)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22260 and BWI76_RS22265 overlap by 4 nucleotides BWI76_RS22265 and BWI76_RS22270 are separated by 254 nucleotides
BWI76_RS22260: BWI76_RS22260 - anaerobic nitric oxide reductase flavorubredoxin, at 4,638,822 to 4,640,267
_RS22260
BWI76_RS22265: BWI76_RS22265 - NADH:flavorubredoxin oxidoreductase, at 4,640,264 to 4,641,397
_RS22265
BWI76_RS22270: BWI76_RS22270 - nickel transporter, at 4,641,652 to 4,642,665
_RS22270
Position (kb) 4640
4641
4642 Strain fitness (log2 ratio) -1
0
1 at 4639.302 kb on + strand, within BWI76_RS22260 at 4639.339 kb on - strand, within BWI76_RS22260 at 4639.339 kb on - strand, within BWI76_RS22260 at 4639.405 kb on - strand, within BWI76_RS22260 at 4639.667 kb on - strand, within BWI76_RS22260 at 4639.897 kb on - strand, within BWI76_RS22260 at 4639.955 kb on + strand, within BWI76_RS22260 at 4640.399 kb on - strand, within BWI76_RS22265 at 4640.663 kb on + strand, within BWI76_RS22265 at 4640.894 kb on - strand, within BWI76_RS22265 at 4641.366 kb on + strand at 4641.391 kb on - strand at 4641.528 kb on - strand at 4641.651 kb on - strand at 4641.721 kb on - strand at 4641.776 kb on - strand, within BWI76_RS22270 at 4641.839 kb on + strand, within BWI76_RS22270 at 4641.847 kb on - strand, within BWI76_RS22270 at 4641.893 kb on + strand, within BWI76_RS22270 at 4641.930 kb on - strand, within BWI76_RS22270 at 4642.218 kb on + strand, within BWI76_RS22270 at 4642.226 kb on - strand, within BWI76_RS22270
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/2) remove 4,639,302 + BWI76_RS22260 0.33 -1.1 4,639,339 - BWI76_RS22260 0.36 -1.0 4,639,339 - BWI76_RS22260 0.36 -0.1 4,639,405 - BWI76_RS22260 0.40 -0.1 4,639,667 - BWI76_RS22260 0.58 -0.1 4,639,897 - BWI76_RS22260 0.74 -0.2 4,639,955 + BWI76_RS22260 0.78 -0.4 4,640,399 - BWI76_RS22265 0.12 -0.9 4,640,663 + BWI76_RS22265 0.35 +0.0 4,640,894 - BWI76_RS22265 0.56 -1.5 4,641,366 + -0.2 4,641,391 - -0.6 4,641,528 - +0.0 4,641,651 - +0.4 4,641,721 - -0.1 4,641,776 - BWI76_RS22270 0.12 +1.2 4,641,839 + BWI76_RS22270 0.18 -0.4 4,641,847 - BWI76_RS22270 0.19 -0.1 4,641,893 + BWI76_RS22270 0.24 -0.3 4,641,930 - BWI76_RS22270 0.27 +0.6 4,642,218 + BWI76_RS22270 0.56 +0.0 4,642,226 - BWI76_RS22270 0.57 -0.4
Or see this region's nucleotide sequence