Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12470

Experiment: Dilute LB (1/2)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12450 and BWI76_RS12455 are separated by 51 nucleotidesBWI76_RS12455 and BWI76_RS12460 overlap by 1 nucleotidesBWI76_RS12460 and BWI76_RS12465 are separated by 19 nucleotidesBWI76_RS12465 and BWI76_RS12470 are separated by 33 nucleotidesBWI76_RS12470 and BWI76_RS12475 are separated by 100 nucleotides BWI76_RS12450: BWI76_RS12450 - phage shock protein PspA, at 2,602,661 to 2,603,329 _RS12450 BWI76_RS12455: BWI76_RS12455 - envelope stress response membrane protein PspB, at 2,603,381 to 2,603,605 _RS12455 BWI76_RS12460: BWI76_RS12460 - envelope stress response membrane protein PspC, at 2,603,605 to 2,603,964 _RS12460 BWI76_RS12465: BWI76_RS12465 - phage shock protein D, at 2,603,984 to 2,604,214 _RS12465 BWI76_RS12470: BWI76_RS12470 - thiosulfate sulfurtransferase PspE, at 2,604,248 to 2,604,562 _RS12470 BWI76_RS12475: BWI76_RS12475 - hypothetical protein, at 2,604,663 to 2,606,060 _RS12475 Position (kb) 2604 2605Strain fitness (log2 ratio) -2 -1 0 1at 2603.698 kb on - strand, within BWI76_RS12460at 2603.731 kb on - strand, within BWI76_RS12460at 2604.075 kb on - strand, within BWI76_RS12465at 2604.208 kb on + strandat 2604.307 kb on + strand, within BWI76_RS12470at 2604.762 kb on + strandat 2604.764 kb on + strandat 2604.787 kb on - strandat 2604.787 kb on - strandat 2604.931 kb on - strand, within BWI76_RS12475at 2605.111 kb on - strand, within BWI76_RS12475at 2605.161 kb on - strand, within BWI76_RS12475at 2605.223 kb on - strand, within BWI76_RS12475at 2605.375 kb on + strand, within BWI76_RS12475at 2605.385 kb on + strand, within BWI76_RS12475

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Per-strain Table

Position Strand Gene LocusTag Fraction Dilute LB (1/2)
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2,603,698 - BWI76_RS12460 0.26 +0.5
2,603,731 - BWI76_RS12460 0.35 -0.0
2,604,075 - BWI76_RS12465 0.39 +0.8
2,604,208 + +0.3
2,604,307 + BWI76_RS12470 0.19 -2.3
2,604,762 + +0.5
2,604,764 + -0.3
2,604,787 - -0.1
2,604,787 - -0.6
2,604,931 - BWI76_RS12475 0.19 -0.2
2,605,111 - BWI76_RS12475 0.32 -1.0
2,605,161 - BWI76_RS12475 0.36 +0.0
2,605,223 - BWI76_RS12475 0.40 +0.0
2,605,375 + BWI76_RS12475 0.51 +0.1
2,605,385 + BWI76_RS12475 0.52 -0.1

Or see this region's nucleotide sequence