Experiment: Dilute LB (1/2)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10525 and BWI76_RS10530 are separated by 143 nucleotides BWI76_RS10530 and BWI76_RS10535 overlap by 1 nucleotides BWI76_RS10535 and BWI76_RS10540 are separated by 16 nucleotides
BWI76_RS10525: BWI76_RS10525 - cytosine/purines uracil thiamine allantoin permease, at 2,223,822 to 2,225,309
_RS10525
BWI76_RS10530: BWI76_RS10530 - GntR family transcriptional regulator with, at 2,225,453 to 2,226,169
_RS10530
BWI76_RS10535: BWI76_RS10535 - Asp/Glu/hydantoin racemase, at 2,226,169 to 2,226,912
_RS10535
BWI76_RS10540: BWI76_RS10540 - allantoinase, at 2,226,929 to 2,227,861
_RS10540
Position (kb)
2226
2227 Strain fitness (log2 ratio)
-1
0
1 at 2225.283 kb on - strand at 2225.358 kb on - strand at 2225.716 kb on - strand, within BWI76_RS10530 at 2225.939 kb on + strand, within BWI76_RS10530 at 2226.330 kb on - strand, within BWI76_RS10535 at 2226.357 kb on + strand, within BWI76_RS10535 at 2226.595 kb on - strand, within BWI76_RS10535 at 2226.975 kb on + strand at 2227.349 kb on + strand, within BWI76_RS10540 at 2227.392 kb on - strand, within BWI76_RS10540 at 2227.618 kb on - strand, within BWI76_RS10540 at 2227.644 kb on - strand, within BWI76_RS10540 at 2227.667 kb on - strand, within BWI76_RS10540 at 2227.754 kb on - strand, within BWI76_RS10540 at 2227.888 kb on + strand at 2227.909 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/2) remove 2,225,283 - +0.4 2,225,358 - +0.2 2,225,716 - BWI76_RS10530 0.37 -0.2 2,225,939 + BWI76_RS10530 0.68 -0.4 2,226,330 - BWI76_RS10535 0.22 -0.2 2,226,357 + BWI76_RS10535 0.25 -1.2 2,226,595 - BWI76_RS10535 0.57 -0.7 2,226,975 + -0.9 2,227,349 + BWI76_RS10540 0.45 +1.1 2,227,392 - BWI76_RS10540 0.50 -0.7 2,227,618 - BWI76_RS10540 0.74 +0.1 2,227,644 - BWI76_RS10540 0.77 +0.1 2,227,667 - BWI76_RS10540 0.79 +0.4 2,227,754 - BWI76_RS10540 0.88 -0.5 2,227,888 + -0.4 2,227,909 - -0.0
Or see this region's nucleotide sequence