Experiment: Dilute LB (1/2)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00655 and BWI76_RS00660 overlap by 1 nucleotides BWI76_RS00660 and BWI76_RS00665 are separated by 96 nucleotides
BWI76_RS00655: BWI76_RS00655 - inner-membrane translocator, at 133,297 to 134,301
_RS00655
BWI76_RS00660: BWI76_RS00660 - ABC transporter, at 134,301 to 135,812
_RS00660
BWI76_RS00665: BWI76_RS00665 - rhamnose ABC transporter substrate-binding protein, at 135,909 to 136,895
_RS00665
Position (kb)
134
135
136 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 133.344 kb on + strand at 133.344 kb on + strand at 133.472 kb on - strand at 133.729 kb on + strand, within BWI76_RS00655 at 133.737 kb on - strand, within BWI76_RS00655 at 133.761 kb on + strand, within BWI76_RS00655 at 133.771 kb on - strand, within BWI76_RS00655 at 133.796 kb on + strand, within BWI76_RS00655 at 133.866 kb on - strand, within BWI76_RS00655 at 133.935 kb on - strand, within BWI76_RS00655 at 133.974 kb on - strand, within BWI76_RS00655 at 134.062 kb on - strand, within BWI76_RS00655 at 134.085 kb on + strand, within BWI76_RS00655 at 134.125 kb on - strand, within BWI76_RS00655 at 134.187 kb on + strand, within BWI76_RS00655 at 134.209 kb on + strand at 134.370 kb on + strand at 134.485 kb on + strand, within BWI76_RS00660 at 134.488 kb on - strand, within BWI76_RS00660 at 134.896 kb on + strand, within BWI76_RS00660 at 134.975 kb on + strand, within BWI76_RS00660 at 135.066 kb on + strand, within BWI76_RS00660 at 135.149 kb on - strand, within BWI76_RS00660 at 135.151 kb on - strand at 135.207 kb on - strand, within BWI76_RS00660 at 135.360 kb on + strand, within BWI76_RS00660 at 135.436 kb on + strand, within BWI76_RS00660 at 135.563 kb on + strand, within BWI76_RS00660 at 135.596 kb on + strand, within BWI76_RS00660 at 135.641 kb on + strand, within BWI76_RS00660 at 135.649 kb on - strand, within BWI76_RS00660 at 135.766 kb on + strand at 135.834 kb on + strand at 135.844 kb on + strand at 135.878 kb on + strand at 135.878 kb on + strand at 135.878 kb on + strand at 135.880 kb on + strand at 135.886 kb on - strand at 135.905 kb on - strand at 136.080 kb on + strand, within BWI76_RS00665 at 136.270 kb on + strand, within BWI76_RS00665 at 136.272 kb on - strand, within BWI76_RS00665 at 136.348 kb on + strand, within BWI76_RS00665 at 136.387 kb on - strand, within BWI76_RS00665 at 136.409 kb on - strand, within BWI76_RS00665 at 136.525 kb on - strand, within BWI76_RS00665 at 136.525 kb on - strand, within BWI76_RS00665 at 136.571 kb on + strand, within BWI76_RS00665 at 136.579 kb on - strand, within BWI76_RS00665 at 136.696 kb on + strand, within BWI76_RS00665 at 136.715 kb on - strand, within BWI76_RS00665
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/2) remove 133,344 + -0.5 133,344 + -0.6 133,472 - +1.8 133,729 + BWI76_RS00655 0.43 -0.3 133,737 - BWI76_RS00655 0.44 +0.0 133,761 + BWI76_RS00655 0.46 -0.7 133,771 - BWI76_RS00655 0.47 +0.6 133,796 + BWI76_RS00655 0.50 -0.5 133,866 - BWI76_RS00655 0.57 -0.9 133,935 - BWI76_RS00655 0.63 +0.3 133,974 - BWI76_RS00655 0.67 +0.5 134,062 - BWI76_RS00655 0.76 -0.6 134,085 + BWI76_RS00655 0.78 -0.3 134,125 - BWI76_RS00655 0.82 -0.6 134,187 + BWI76_RS00655 0.89 +0.2 134,209 + -0.1 134,370 + +0.2 134,485 + BWI76_RS00660 0.12 -1.2 134,488 - BWI76_RS00660 0.12 -0.4 134,896 + BWI76_RS00660 0.39 -1.4 134,975 + BWI76_RS00660 0.45 -0.9 135,066 + BWI76_RS00660 0.51 -2.4 135,149 - BWI76_RS00660 0.56 -0.5 135,151 - +1.8 135,207 - BWI76_RS00660 0.60 +0.0 135,360 + BWI76_RS00660 0.70 +0.0 135,436 + BWI76_RS00660 0.75 -1.2 135,563 + BWI76_RS00660 0.83 -0.6 135,596 + BWI76_RS00660 0.86 -1.6 135,641 + BWI76_RS00660 0.89 -0.6 135,649 - BWI76_RS00660 0.89 -2.0 135,766 + +0.2 135,834 + -0.1 135,844 + -0.9 135,878 + +0.3 135,878 + +0.1 135,878 + +0.6 135,880 + -0.6 135,886 - -0.2 135,905 - -0.8 136,080 + BWI76_RS00665 0.17 -2.2 136,270 + BWI76_RS00665 0.37 -0.1 136,272 - BWI76_RS00665 0.37 +0.3 136,348 + BWI76_RS00665 0.44 +0.1 136,387 - BWI76_RS00665 0.48 +0.1 136,409 - BWI76_RS00665 0.51 +1.2 136,525 - BWI76_RS00665 0.62 +1.0 136,525 - BWI76_RS00665 0.62 +0.1 136,571 + BWI76_RS00665 0.67 -0.2 136,579 - BWI76_RS00665 0.68 -0.4 136,696 + BWI76_RS00665 0.80 -0.4 136,715 - BWI76_RS00665 0.82 +0.2
Or see this region's nucleotide sequence