Experiment: Varel_Bryant_medium_Glucose with Dimetridazole 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2769 and BT2770 are separated by 12 nucleotides BT2770 and BT2771 overlap by 4 nucleotides BT2771 and BT2772 are separated by 2 nucleotides
BT2769: BT2769 - putative serine/threonine-protein kinase pknB (NCBI ptt file), at 3,428,346 to 3,432,137
BT2769
BT2770: BT2770 - hypothetical protein (NCBI ptt file), at 3,432,150 to 3,432,677
BT2770
BT2771: BT2771 - putative serine/threonine-protein kinase pknB (NCBI ptt file), at 3,432,674 to 3,434,038
BT2771
BT2772: BT2772 - putative protein phosphatase (NCBI ptt file), at 3,434,041 to 3,435,690
BT2772
Position (kb)
3432
3433
3434
3435 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3431.715 kb on - strand, within BT2769 at 3431.750 kb on + strand, within BT2769 at 3431.751 kb on - strand, within BT2769 at 3431.751 kb on - strand, within BT2769 at 3431.782 kb on - strand at 3431.786 kb on + strand at 3431.787 kb on - strand at 3431.787 kb on - strand at 3431.842 kb on - strand at 3431.843 kb on + strand at 3431.843 kb on + strand at 3431.845 kb on + strand at 3431.846 kb on - strand at 3431.846 kb on - strand at 3431.859 kb on - strand at 3431.887 kb on + strand at 3431.891 kb on + strand at 3431.891 kb on + strand at 3431.914 kb on + strand at 3431.946 kb on - strand at 3431.990 kb on + strand at 3432.011 kb on + strand at 3432.031 kb on - strand at 3432.050 kb on - strand at 3432.063 kb on + strand at 3432.068 kb on - strand at 3432.113 kb on - strand at 3432.116 kb on + strand at 3432.116 kb on + strand at 3432.117 kb on - strand at 3432.117 kb on - strand at 3432.151 kb on + strand at 3432.257 kb on - strand, within BT2770 at 3432.260 kb on + strand, within BT2770 at 3432.281 kb on + strand, within BT2770 at 3432.294 kb on + strand, within BT2770 at 3432.335 kb on + strand, within BT2770 at 3432.354 kb on + strand, within BT2770 at 3432.355 kb on - strand, within BT2770 at 3432.363 kb on - strand, within BT2770 at 3432.410 kb on - strand, within BT2770 at 3432.425 kb on + strand, within BT2770 at 3432.481 kb on - strand, within BT2770 at 3432.488 kb on + strand, within BT2770 at 3432.489 kb on - strand, within BT2770 at 3432.528 kb on - strand, within BT2770 at 3432.542 kb on + strand, within BT2770 at 3432.543 kb on - strand, within BT2770 at 3432.571 kb on - strand, within BT2770 at 3432.574 kb on + strand, within BT2770 at 3432.574 kb on + strand, within BT2770 at 3432.575 kb on - strand, within BT2770 at 3432.579 kb on - strand, within BT2770 at 3432.633 kb on + strand at 3432.677 kb on + strand at 3432.678 kb on - strand at 3432.679 kb on + strand at 3432.686 kb on + strand at 3432.691 kb on + strand at 3432.731 kb on + strand at 3432.731 kb on + strand at 3432.732 kb on - strand at 3432.733 kb on + strand at 3432.734 kb on - strand at 3432.785 kb on + strand at 3432.785 kb on + strand at 3432.871 kb on + strand, within BT2771 at 3432.884 kb on - strand, within BT2771 at 3433.049 kb on + strand, within BT2771 at 3433.064 kb on + strand, within BT2771 at 3433.064 kb on + strand, within BT2771 at 3433.085 kb on - strand, within BT2771 at 3433.087 kb on + strand, within BT2771 at 3433.087 kb on + strand, within BT2771 at 3433.088 kb on - strand, within BT2771 at 3433.123 kb on + strand, within BT2771 at 3433.139 kb on - strand, within BT2771 at 3433.168 kb on + strand, within BT2771 at 3433.187 kb on + strand, within BT2771 at 3433.235 kb on + strand, within BT2771 at 3433.343 kb on + strand, within BT2771 at 3433.354 kb on + strand, within BT2771 at 3433.407 kb on + strand, within BT2771 at 3433.450 kb on + strand, within BT2771 at 3433.452 kb on + strand, within BT2771 at 3433.453 kb on - strand, within BT2771 at 3433.457 kb on + strand, within BT2771 at 3433.457 kb on + strand, within BT2771 at 3433.458 kb on - strand, within BT2771 at 3433.476 kb on + strand, within BT2771 at 3433.496 kb on + strand, within BT2771 at 3433.496 kb on + strand, within BT2771 at 3433.496 kb on + strand, within BT2771 at 3433.529 kb on - strand, within BT2771 at 3433.529 kb on - strand, within BT2771 at 3433.529 kb on - strand, within BT2771 at 3433.529 kb on - strand, within BT2771 at 3433.549 kb on + strand, within BT2771 at 3433.550 kb on - strand, within BT2771 at 3433.550 kb on - strand, within BT2771 at 3433.604 kb on + strand, within BT2771 at 3433.615 kb on + strand, within BT2771 at 3433.619 kb on - strand, within BT2771 at 3433.638 kb on - strand, within BT2771 at 3433.642 kb on + strand, within BT2771 at 3433.642 kb on + strand, within BT2771 at 3433.642 kb on + strand, within BT2771 at 3433.643 kb on - strand, within BT2771 at 3433.643 kb on - strand, within BT2771 at 3433.654 kb on + strand, within BT2771 at 3433.678 kb on + strand, within BT2771 at 3433.678 kb on + strand, within BT2771 at 3433.682 kb on + strand, within BT2771 at 3433.682 kb on + strand, within BT2771 at 3433.682 kb on + strand, within BT2771 at 3433.683 kb on - strand, within BT2771 at 3433.683 kb on - strand, within BT2771 at 3433.684 kb on + strand, within BT2771 at 3433.685 kb on - strand, within BT2771 at 3433.686 kb on + strand, within BT2771 at 3433.686 kb on + strand, within BT2771 at 3433.686 kb on + strand, within BT2771 at 3433.687 kb on - strand, within BT2771 at 3433.788 kb on + strand, within BT2771 at 3433.807 kb on + strand, within BT2771 at 3433.847 kb on + strand, within BT2771 at 3433.848 kb on - strand, within BT2771 at 3433.934 kb on - strand at 3433.983 kb on + strand at 3434.009 kb on + strand at 3434.029 kb on - strand at 3434.043 kb on - strand at 3434.064 kb on + strand at 3434.064 kb on + strand at 3434.071 kb on - strand at 3434.089 kb on + strand at 3434.131 kb on + strand at 3434.136 kb on + strand at 3434.210 kb on + strand, within BT2772 at 3434.223 kb on - strand, within BT2772 at 3434.357 kb on + strand, within BT2772 at 3434.357 kb on + strand, within BT2772 at 3434.403 kb on + strand, within BT2772 at 3434.403 kb on + strand, within BT2772 at 3434.422 kb on - strand, within BT2772 at 3434.425 kb on + strand, within BT2772 at 3434.425 kb on + strand, within BT2772 at 3434.434 kb on + strand, within BT2772 at 3434.611 kb on - strand, within BT2772 at 3434.621 kb on + strand, within BT2772 at 3434.622 kb on - strand, within BT2772 at 3434.626 kb on + strand, within BT2772 at 3434.654 kb on - strand, within BT2772 at 3434.698 kb on - strand, within BT2772 at 3434.709 kb on + strand, within BT2772 at 3434.749 kb on - strand, within BT2772 at 3434.859 kb on + strand, within BT2772 at 3434.859 kb on + strand, within BT2772 at 3434.859 kb on + strand at 3434.863 kb on + strand, within BT2772 at 3434.863 kb on + strand, within BT2772 at 3434.881 kb on + strand, within BT2772 at 3434.884 kb on + strand, within BT2772 at 3434.884 kb on + strand, within BT2772 at 3434.885 kb on - strand, within BT2772 at 3434.965 kb on - strand, within BT2772 at 3434.998 kb on + strand at 3435.001 kb on + strand, within BT2772
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Dimetridazole 0.025 mM remove 3,431,715 - BT2769 0.89 -1.7 3,431,750 + BT2769 0.90 -1.7 3,431,751 - BT2769 0.90 -3.0 3,431,751 - BT2769 0.90 -2.2 3,431,782 - +0.4 3,431,786 + -2.7 3,431,787 - -0.7 3,431,787 - -1.2 3,431,842 - -0.2 3,431,843 + -1.5 3,431,843 + +0.6 3,431,845 + -0.4 3,431,846 - -1.2 3,431,846 - -1.2 3,431,859 - -1.5 3,431,887 + -2.2 3,431,891 + -0.9 3,431,891 + -1.2 3,431,914 + -1.2 3,431,946 - -0.2 3,431,990 + -1.2 3,432,011 + -1.1 3,432,031 - -1.7 3,432,050 - -0.6 3,432,063 + -0.2 3,432,068 - -4.0 3,432,113 - -1.2 3,432,116 + -0.7 3,432,116 + -1.0 3,432,117 - +0.6 3,432,117 - +0.8 3,432,151 + -1.6 3,432,257 - BT2770 0.20 +0.3 3,432,260 + BT2770 0.21 -0.9 3,432,281 + BT2770 0.25 -2.5 3,432,294 + BT2770 0.27 -4.3 3,432,335 + BT2770 0.35 -1.1 3,432,354 + BT2770 0.39 -1.7 3,432,355 - BT2770 0.39 -0.9 3,432,363 - BT2770 0.40 -0.2 3,432,410 - BT2770 0.49 -0.2 3,432,425 + BT2770 0.52 +0.4 3,432,481 - BT2770 0.63 -0.9 3,432,488 + BT2770 0.64 -3.0 3,432,489 - BT2770 0.64 -1.2 3,432,528 - BT2770 0.72 -1.2 3,432,542 + BT2770 0.74 +0.4 3,432,543 - BT2770 0.74 -1.7 3,432,571 - BT2770 0.80 -1.0 3,432,574 + BT2770 0.80 +0.8 3,432,574 + BT2770 0.80 -1.2 3,432,575 - BT2770 0.80 -1.7 3,432,579 - BT2770 0.81 -1.7 3,432,633 + -2.7 3,432,677 + -2.0 3,432,678 - -0.2 3,432,679 + -0.6 3,432,686 + +1.8 3,432,691 + -0.4 3,432,731 + -1.7 3,432,731 + -1.5 3,432,732 - -1.7 3,432,733 + -1.7 3,432,734 - -0.2 3,432,785 + -2.0 3,432,785 + -0.7 3,432,871 + BT2771 0.14 -3.4 3,432,884 - BT2771 0.15 -1.2 3,433,049 + BT2771 0.27 -3.5 3,433,064 + BT2771 0.29 -2.1 3,433,064 + BT2771 0.29 -0.8 3,433,085 - BT2771 0.30 -1.2 3,433,087 + BT2771 0.30 -1.6 3,433,087 + BT2771 0.30 -1.7 3,433,088 - BT2771 0.30 -0.6 3,433,123 + BT2771 0.33 -0.6 3,433,139 - BT2771 0.34 -3.1 3,433,168 + BT2771 0.36 -0.0 3,433,187 + BT2771 0.38 -0.9 3,433,235 + BT2771 0.41 -1.3 3,433,343 + BT2771 0.49 +0.8 3,433,354 + BT2771 0.50 -1.1 3,433,407 + BT2771 0.54 -1.7 3,433,450 + BT2771 0.57 -2.5 3,433,452 + BT2771 0.57 -2.1 3,433,453 - BT2771 0.57 -3.9 3,433,457 + BT2771 0.57 -1.6 3,433,457 + BT2771 0.57 -1.6 3,433,458 - BT2771 0.57 -1.2 3,433,476 + BT2771 0.59 -0.8 3,433,496 + BT2771 0.60 -1.2 3,433,496 + BT2771 0.60 -1.7 3,433,496 + BT2771 0.60 -1.3 3,433,529 - BT2771 0.63 -0.2 3,433,529 - BT2771 0.63 -0.6 3,433,529 - BT2771 0.63 +0.4 3,433,529 - BT2771 0.63 -1.7 3,433,549 + BT2771 0.64 -2.7 3,433,550 - BT2771 0.64 -2.1 3,433,550 - BT2771 0.64 -2.5 3,433,604 + BT2771 0.68 -0.2 3,433,615 + BT2771 0.69 -2.2 3,433,619 - BT2771 0.69 +1.4 3,433,638 - BT2771 0.71 -1.5 3,433,642 + BT2771 0.71 -1.7 3,433,642 + BT2771 0.71 -1.2 3,433,642 + BT2771 0.71 -2.2 3,433,643 - BT2771 0.71 -0.2 3,433,643 - BT2771 0.71 -0.7 3,433,654 + BT2771 0.72 +1.4 3,433,678 + BT2771 0.74 -2.3 3,433,678 + BT2771 0.74 +1.4 3,433,682 + BT2771 0.74 -1.2 3,433,682 + BT2771 0.74 -1.3 3,433,682 + BT2771 0.74 +1.4 3,433,683 - BT2771 0.74 -1.1 3,433,683 - BT2771 0.74 -1.2 3,433,684 + BT2771 0.74 -1.7 3,433,685 - BT2771 0.74 -2.7 3,433,686 + BT2771 0.74 -4.1 3,433,686 + BT2771 0.74 -2.7 3,433,686 + BT2771 0.74 -2.9 3,433,687 - BT2771 0.74 -1.7 3,433,788 + BT2771 0.82 -2.6 3,433,807 + BT2771 0.83 -1.7 3,433,847 + BT2771 0.86 -2.5 3,433,848 - BT2771 0.86 -1.1 3,433,934 - -1.5 3,433,983 + -0.2 3,434,009 + -1.0 3,434,029 - -1.2 3,434,043 - +2.0 3,434,064 + -3.5 3,434,064 + -1.4 3,434,071 - -1.0 3,434,089 + -1.3 3,434,131 + +1.4 3,434,136 + +1.4 3,434,210 + BT2772 0.10 -2.5 3,434,223 - BT2772 0.11 -0.9 3,434,357 + BT2772 0.19 -1.3 3,434,357 + BT2772 0.19 -1.7 3,434,403 + BT2772 0.22 -1.6 3,434,403 + BT2772 0.22 -1.1 3,434,422 - BT2772 0.23 -1.2 3,434,425 + BT2772 0.23 -1.3 3,434,425 + BT2772 0.23 +0.7 3,434,434 + BT2772 0.24 -0.2 3,434,611 - BT2772 0.35 -0.7 3,434,621 + BT2772 0.35 -1.7 3,434,622 - BT2772 0.35 +0.8 3,434,626 + BT2772 0.35 -1.5 3,434,654 - BT2772 0.37 -0.7 3,434,698 - BT2772 0.40 -1.5 3,434,709 + BT2772 0.40 +0.8 3,434,749 - BT2772 0.43 -0.2 3,434,859 + BT2772 0.50 -2.7 3,434,859 + BT2772 0.50 -1.0 3,434,859 + -0.2 3,434,863 + BT2772 0.50 -1.6 3,434,863 + BT2772 0.50 -1.7 3,434,881 + BT2772 0.51 -2.0 3,434,884 + BT2772 0.51 -0.6 3,434,884 + BT2772 0.51 -0.9 3,434,885 - BT2772 0.51 -0.2 3,434,965 - BT2772 0.56 -3.2 3,434,998 + -1.2 3,435,001 + BT2772 0.58 -1.5
Or see this region's nucleotide sequence