Experiment: Pyruvate-Sulfite (60-20mM) with Molybdate 100uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phnE and phnC overlap by 13 nucleotides phnC and DVU1937 are separated by 76 nucleotides DVU1937 and DVU1938 are separated by 281 nucleotides DVU1938 and DVU1939 are separated by 45 nucleotides
DVU1935: phnE - phosphonate ABC transporter, permease protein (TIGR), at 2,007,946 to 2,008,743
phnE
DVU1936: phnC - phosphonate ABC transporter, ATP-binding protein (TIGR), at 2,008,731 to 2,009,528
phnC
DVU1937: DVU1937 - phosphonate ABC transporter, periplasmic phosphonate-binding protein, putative (TIGR), at 2,009,605 to 2,010,618
DVU1937
DVU1938: DVU1938 - hypothetical protein (TIGR), at 2,010,900 to 2,011,175
DVU1938
DVU1939: DVU1939 - anaerobic glycerol-3-phosphate dehydrogenase, B subunit, putative (TIGR), at 2,011,221 to 2,012,504
DVU1939
Position (kb)
2009
2010
2011 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2008.684 kb on - strand at 2008.912 kb on + strand, within phnC at 2009.256 kb on + strand, within phnC at 2009.279 kb on - strand, within phnC at 2009.291 kb on - strand, within phnC at 2009.397 kb on - strand, within phnC at 2009.416 kb on - strand, within phnC at 2009.445 kb on + strand, within phnC at 2009.455 kb on + strand at 2009.463 kb on - strand at 2009.528 kb on + strand at 2009.674 kb on + strand at 2009.711 kb on + strand, within DVU1937 at 2009.940 kb on - strand, within DVU1937 at 2009.997 kb on + strand, within DVU1937 at 2010.005 kb on - strand, within DVU1937 at 2010.005 kb on - strand, within DVU1937 at 2010.023 kb on + strand, within DVU1937 at 2010.023 kb on + strand, within DVU1937 at 2010.045 kb on + strand, within DVU1937 at 2010.116 kb on + strand, within DVU1937 at 2010.116 kb on + strand, within DVU1937 at 2010.122 kb on + strand, within DVU1937 at 2010.144 kb on + strand, within DVU1937 at 2010.149 kb on + strand, within DVU1937 at 2010.193 kb on - strand, within DVU1937 at 2010.247 kb on + strand, within DVU1937 at 2010.271 kb on - strand, within DVU1937 at 2010.273 kb on - strand, within DVU1937 at 2010.310 kb on - strand, within DVU1937 at 2010.317 kb on + strand, within DVU1937 at 2010.332 kb on + strand, within DVU1937 at 2010.332 kb on + strand, within DVU1937 at 2010.332 kb on + strand, within DVU1937 at 2010.340 kb on - strand, within DVU1937 at 2010.340 kb on - strand, within DVU1937 at 2010.340 kb on - strand, within DVU1937 at 2010.552 kb on - strand at 2010.658 kb on - strand at 2010.658 kb on - strand at 2010.693 kb on + strand at 2010.693 kb on + strand at 2011.040 kb on + strand, within DVU1938 at 2011.074 kb on - strand, within DVU1938 at 2011.100 kb on + strand, within DVU1938 at 2011.149 kb on + strand at 2011.166 kb on - strand at 2011.226 kb on - strand at 2011.228 kb on + strand at 2011.228 kb on + strand at 2011.236 kb on - strand at 2011.236 kb on - strand at 2011.405 kb on + strand, within DVU1939 at 2011.443 kb on - strand, within DVU1939 at 2011.445 kb on + strand, within DVU1939 at 2011.445 kb on + strand, within DVU1939 at 2011.451 kb on + strand, within DVU1939 at 2011.535 kb on + strand, within DVU1939 at 2011.565 kb on + strand, within DVU1939 at 2011.600 kb on - strand, within DVU1939
Per-strain Table
Position Strand Gene LocusTag Fraction Pyruvate-Sulfite (60-20mM) with Molybdate 100uM remove 2,008,684 - +1.4 2,008,912 + phnC DVU1936 0.23 +1.0 2,009,256 + phnC DVU1936 0.66 +1.7 2,009,279 - phnC DVU1936 0.69 +1.0 2,009,291 - phnC DVU1936 0.70 -1.7 2,009,397 - phnC DVU1936 0.83 +1.0 2,009,416 - phnC DVU1936 0.86 +2.8 2,009,445 + phnC DVU1936 0.89 +1.5 2,009,455 + +1.1 2,009,463 - +0.9 2,009,528 + +0.9 2,009,674 + +0.6 2,009,711 + DVU1937 0.10 +0.9 2,009,940 - DVU1937 0.33 +1.4 2,009,997 + DVU1937 0.39 +2.1 2,010,005 - DVU1937 0.39 +1.0 2,010,005 - DVU1937 0.39 +1.0 2,010,023 + DVU1937 0.41 +1.2 2,010,023 + DVU1937 0.41 +1.1 2,010,045 + DVU1937 0.43 -1.0 2,010,116 + DVU1937 0.50 +0.7 2,010,116 + DVU1937 0.50 +1.3 2,010,122 + DVU1937 0.51 +2.0 2,010,144 + DVU1937 0.53 +1.3 2,010,149 + DVU1937 0.54 -0.2 2,010,193 - DVU1937 0.58 +1.0 2,010,247 + DVU1937 0.63 +1.2 2,010,271 - DVU1937 0.66 +1.1 2,010,273 - DVU1937 0.66 +1.6 2,010,310 - DVU1937 0.70 +2.3 2,010,317 + DVU1937 0.70 +0.8 2,010,332 + DVU1937 0.72 -0.3 2,010,332 + DVU1937 0.72 +1.1 2,010,332 + DVU1937 0.72 +1.3 2,010,340 - DVU1937 0.72 -2.7 2,010,340 - DVU1937 0.72 +1.4 2,010,340 - DVU1937 0.72 -2.1 2,010,552 - -0.3 2,010,658 - +0.6 2,010,658 - +1.6 2,010,693 + +0.9 2,010,693 + +0.7 2,011,040 + DVU1938 0.51 -0.8 2,011,074 - DVU1938 0.63 -0.1 2,011,100 + DVU1938 0.72 +0.7 2,011,149 + -0.8 2,011,166 - -0.9 2,011,226 - -0.7 2,011,228 + -1.1 2,011,228 + +0.5 2,011,236 - -0.3 2,011,236 - +0.4 2,011,405 + DVU1939 0.14 +1.6 2,011,443 - DVU1939 0.17 -0.9 2,011,445 + DVU1939 0.17 -0.2 2,011,445 + DVU1939 0.17 +1.1 2,011,451 + DVU1939 0.18 -0.1 2,011,535 + DVU1939 0.24 +0.5 2,011,565 + DVU1939 0.27 +0.1 2,011,600 - DVU1939 0.30 +1.6
Or see this region's nucleotide sequence