Experiment: L-Proline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4186 and Pf6N2E2_4187 are separated by 163 nucleotides
Pf6N2E2_4186 - Glycine oxidase ThiO (EC 1.4.3.19), at 4,871,092 to 4,872,138
_4186
Pf6N2E2_4187 - Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-), at 4,872,302 to 4,873,189
_4187
Position (kb)
4871
4872
4873 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4870.248 kb on - strand at 4870.248 kb on - strand at 4870.313 kb on + strand at 4870.397 kb on + strand at 4870.398 kb on - strand at 4870.461 kb on + strand at 4870.462 kb on - strand at 4870.563 kb on - strand at 4870.563 kb on - strand at 4870.584 kb on + strand at 4870.614 kb on + strand at 4870.615 kb on - strand at 4870.680 kb on + strand at 4870.726 kb on - strand at 4870.795 kb on + strand at 4870.806 kb on - strand at 4870.898 kb on + strand at 4870.988 kb on + strand at 4871.036 kb on + strand at 4871.037 kb on - strand at 4871.093 kb on + strand at 4871.094 kb on - strand at 4871.157 kb on + strand at 4871.157 kb on + strand at 4871.222 kb on + strand, within Pf6N2E2_4186 at 4871.222 kb on + strand, within Pf6N2E2_4186 at 4871.222 kb on + strand, within Pf6N2E2_4186 at 4871.222 kb on + strand, within Pf6N2E2_4186 at 4871.222 kb on + strand, within Pf6N2E2_4186 at 4871.222 kb on + strand, within Pf6N2E2_4186 at 4871.222 kb on + strand, within Pf6N2E2_4186 at 4871.222 kb on + strand, within Pf6N2E2_4186 at 4871.222 kb on + strand, within Pf6N2E2_4186 at 4871.223 kb on - strand, within Pf6N2E2_4186 at 4871.223 kb on - strand, within Pf6N2E2_4186 at 4871.223 kb on - strand, within Pf6N2E2_4186 at 4871.223 kb on - strand, within Pf6N2E2_4186 at 4871.223 kb on - strand, within Pf6N2E2_4186 at 4871.224 kb on + strand, within Pf6N2E2_4186 at 4871.224 kb on + strand, within Pf6N2E2_4186 at 4871.224 kb on + strand, within Pf6N2E2_4186 at 4871.224 kb on + strand, within Pf6N2E2_4186 at 4871.224 kb on + strand, within Pf6N2E2_4186 at 4871.224 kb on + strand, within Pf6N2E2_4186 at 4871.224 kb on + strand, within Pf6N2E2_4186 at 4871.225 kb on - strand, within Pf6N2E2_4186 at 4871.246 kb on + strand, within Pf6N2E2_4186 at 4871.305 kb on + strand, within Pf6N2E2_4186 at 4871.305 kb on + strand, within Pf6N2E2_4186 at 4871.305 kb on + strand, within Pf6N2E2_4186 at 4871.305 kb on + strand, within Pf6N2E2_4186 at 4871.305 kb on + strand, within Pf6N2E2_4186 at 4871.306 kb on - strand, within Pf6N2E2_4186 at 4871.306 kb on - strand, within Pf6N2E2_4186 at 4871.306 kb on - strand, within Pf6N2E2_4186 at 4871.306 kb on - strand, within Pf6N2E2_4186 at 4871.306 kb on - strand, within Pf6N2E2_4186 at 4871.306 kb on - strand, within Pf6N2E2_4186 at 4871.403 kb on - strand, within Pf6N2E2_4186 at 4871.498 kb on + strand, within Pf6N2E2_4186 at 4871.499 kb on - strand, within Pf6N2E2_4186 at 4871.527 kb on + strand, within Pf6N2E2_4186 at 4871.587 kb on + strand, within Pf6N2E2_4186 at 4871.587 kb on + strand, within Pf6N2E2_4186 at 4871.587 kb on + strand, within Pf6N2E2_4186 at 4871.656 kb on - strand, within Pf6N2E2_4186 at 4871.656 kb on - strand, within Pf6N2E2_4186 at 4871.735 kb on - strand, within Pf6N2E2_4186 at 4871.765 kb on + strand, within Pf6N2E2_4186 at 4871.862 kb on - strand, within Pf6N2E2_4186 at 4872.072 kb on - strand at 4872.088 kb on + strand at 4872.088 kb on + strand at 4872.089 kb on - strand at 4872.238 kb on + strand at 4872.307 kb on - strand at 4872.360 kb on + strand at 4872.361 kb on - strand at 4872.384 kb on + strand at 4872.438 kb on + strand, within Pf6N2E2_4187 at 4872.438 kb on + strand, within Pf6N2E2_4187 at 4872.438 kb on + strand, within Pf6N2E2_4187 at 4872.438 kb on + strand at 4872.438 kb on + strand, within Pf6N2E2_4187 at 4872.438 kb on + strand, within Pf6N2E2_4187 at 4872.439 kb on - strand, within Pf6N2E2_4187 at 4872.439 kb on - strand, within Pf6N2E2_4187 at 4872.439 kb on - strand, within Pf6N2E2_4187 at 4872.439 kb on - strand, within Pf6N2E2_4187 at 4872.439 kb on - strand, within Pf6N2E2_4187 at 4872.439 kb on - strand, within Pf6N2E2_4187 at 4872.798 kb on + strand, within Pf6N2E2_4187 at 4872.799 kb on - strand, within Pf6N2E2_4187 at 4872.799 kb on - strand, within Pf6N2E2_4187
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (N) remove 4,870,248 - -0.1 4,870,248 - -0.1 4,870,313 + +1.1 4,870,397 + +1.2 4,870,398 - +0.5 4,870,461 + -0.3 4,870,462 - +0.8 4,870,563 - +0.2 4,870,563 - -0.2 4,870,584 + +1.2 4,870,614 + -0.2 4,870,615 - +0.3 4,870,680 + +0.3 4,870,726 - +0.3 4,870,795 + +1.1 4,870,806 - +0.1 4,870,898 + -0.6 4,870,988 + +0.1 4,871,036 + +0.4 4,871,037 - +0.1 4,871,093 + -0.7 4,871,094 - +1.4 4,871,157 + +0.4 4,871,157 + +0.7 4,871,222 + Pf6N2E2_4186 0.12 +1.7 4,871,222 + Pf6N2E2_4186 0.12 +0.9 4,871,222 + Pf6N2E2_4186 0.12 -0.8 4,871,222 + Pf6N2E2_4186 0.12 +0.3 4,871,222 + Pf6N2E2_4186 0.12 -0.3 4,871,222 + Pf6N2E2_4186 0.12 +0.1 4,871,222 + Pf6N2E2_4186 0.12 -0.0 4,871,222 + Pf6N2E2_4186 0.12 +1.1 4,871,222 + Pf6N2E2_4186 0.12 +0.3 4,871,223 - Pf6N2E2_4186 0.13 +0.4 4,871,223 - Pf6N2E2_4186 0.13 +0.3 4,871,223 - Pf6N2E2_4186 0.13 +0.4 4,871,223 - Pf6N2E2_4186 0.13 -0.3 4,871,223 - Pf6N2E2_4186 0.13 +0.3 4,871,224 + Pf6N2E2_4186 0.13 -0.5 4,871,224 + Pf6N2E2_4186 0.13 +0.5 4,871,224 + Pf6N2E2_4186 0.13 -0.8 4,871,224 + Pf6N2E2_4186 0.13 +0.5 4,871,224 + Pf6N2E2_4186 0.13 -0.2 4,871,224 + Pf6N2E2_4186 0.13 +1.0 4,871,224 + Pf6N2E2_4186 0.13 +0.4 4,871,225 - Pf6N2E2_4186 0.13 +1.1 4,871,246 + Pf6N2E2_4186 0.15 +0.3 4,871,305 + Pf6N2E2_4186 0.20 +0.5 4,871,305 + Pf6N2E2_4186 0.20 +1.3 4,871,305 + Pf6N2E2_4186 0.20 -0.3 4,871,305 + Pf6N2E2_4186 0.20 -0.3 4,871,305 + Pf6N2E2_4186 0.20 +0.3 4,871,306 - Pf6N2E2_4186 0.20 -0.6 4,871,306 - Pf6N2E2_4186 0.20 +1.1 4,871,306 - Pf6N2E2_4186 0.20 +1.3 4,871,306 - Pf6N2E2_4186 0.20 +0.3 4,871,306 - Pf6N2E2_4186 0.20 +0.5 4,871,306 - Pf6N2E2_4186 0.20 +0.1 4,871,403 - Pf6N2E2_4186 0.30 -0.1 4,871,498 + Pf6N2E2_4186 0.39 +0.8 4,871,499 - Pf6N2E2_4186 0.39 +0.6 4,871,527 + Pf6N2E2_4186 0.42 +0.4 4,871,587 + Pf6N2E2_4186 0.47 -0.0 4,871,587 + Pf6N2E2_4186 0.47 +0.1 4,871,587 + Pf6N2E2_4186 0.47 +0.5 4,871,656 - Pf6N2E2_4186 0.54 +0.5 4,871,656 - Pf6N2E2_4186 0.54 +0.3 4,871,735 - Pf6N2E2_4186 0.61 +1.1 4,871,765 + Pf6N2E2_4186 0.64 +0.4 4,871,862 - Pf6N2E2_4186 0.74 +0.1 4,872,072 - +0.4 4,872,088 + +0.5 4,872,088 + +0.6 4,872,089 - +0.2 4,872,238 + +0.9 4,872,307 - -1.8 4,872,360 + -1.3 4,872,361 - -2.5 4,872,384 + -2.7 4,872,438 + Pf6N2E2_4187 0.15 -1.4 4,872,438 + Pf6N2E2_4187 0.15 -1.7 4,872,438 + Pf6N2E2_4187 0.15 -1.6 4,872,438 + +1.7 4,872,438 + Pf6N2E2_4187 0.15 -0.8 4,872,438 + Pf6N2E2_4187 0.15 -2.0 4,872,439 - Pf6N2E2_4187 0.15 -1.8 4,872,439 - Pf6N2E2_4187 0.15 -2.7 4,872,439 - Pf6N2E2_4187 0.15 -0.8 4,872,439 - Pf6N2E2_4187 0.15 -2.0 4,872,439 - Pf6N2E2_4187 0.15 -1.4 4,872,439 - Pf6N2E2_4187 0.15 -1.4 4,872,798 + Pf6N2E2_4187 0.56 -0.8 4,872,799 - Pf6N2E2_4187 0.56 -3.0 4,872,799 - Pf6N2E2_4187 0.56 -1.4
Or see this region's nucleotide sequence