Experiment: L-Proline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1059 and Pf6N2E2_1060 are separated by 175 nucleotides Pf6N2E2_1060 and Pf6N2E2_1061 overlap by 26 nucleotides Pf6N2E2_1061 and Pf6N2E2_1062 are separated by 130 nucleotides
Pf6N2E2_1059 - Phosphoglucomutase (EC 5.4.2.2), at 1,235,468 to 1,237,114
_1059
Pf6N2E2_1060 - Pirin, at 1,237,290 to 1,237,985
_1060
Pf6N2E2_1061 - hypothetical protein, at 1,237,960 to 1,238,091
_1061
Pf6N2E2_1062 - UvrD/REP helicase family protein, at 1,238,222 to 1,240,708
_1062
Position (kb)
1237
1238 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1236.443 kb on + strand, within Pf6N2E2_1059 at 1236.443 kb on + strand, within Pf6N2E2_1059 at 1236.443 kb on + strand, within Pf6N2E2_1059 at 1236.444 kb on - strand, within Pf6N2E2_1059 at 1236.465 kb on - strand, within Pf6N2E2_1059 at 1236.572 kb on + strand, within Pf6N2E2_1059 at 1236.573 kb on - strand, within Pf6N2E2_1059 at 1236.573 kb on - strand, within Pf6N2E2_1059 at 1236.573 kb on - strand, within Pf6N2E2_1059 at 1236.913 kb on + strand, within Pf6N2E2_1059 at 1237.012 kb on + strand at 1237.012 kb on + strand at 1237.012 kb on + strand at 1237.012 kb on + strand at 1237.013 kb on - strand at 1237.013 kb on - strand at 1237.016 kb on + strand at 1237.016 kb on + strand at 1237.016 kb on + strand at 1237.016 kb on + strand at 1237.016 kb on + strand at 1237.016 kb on + strand at 1237.017 kb on - strand at 1237.017 kb on - strand at 1237.017 kb on - strand at 1237.017 kb on - strand at 1237.017 kb on - strand at 1237.025 kb on + strand at 1237.026 kb on - strand at 1237.099 kb on + strand at 1237.115 kb on + strand at 1237.116 kb on - strand at 1237.116 kb on - strand at 1237.221 kb on - strand at 1237.344 kb on + strand at 1237.456 kb on + strand, within Pf6N2E2_1060 at 1237.638 kb on + strand, within Pf6N2E2_1060 at 1237.638 kb on + strand, within Pf6N2E2_1060 at 1237.639 kb on - strand, within Pf6N2E2_1060 at 1237.639 kb on - strand, within Pf6N2E2_1060 at 1237.675 kb on + strand, within Pf6N2E2_1060 at 1237.675 kb on + strand, within Pf6N2E2_1060 at 1237.676 kb on - strand, within Pf6N2E2_1060 at 1237.676 kb on - strand, within Pf6N2E2_1060 at 1237.676 kb on - strand, within Pf6N2E2_1060 at 1237.676 kb on - strand, within Pf6N2E2_1060 at 1237.676 kb on - strand, within Pf6N2E2_1060 at 1237.716 kb on + strand, within Pf6N2E2_1060 at 1237.717 kb on - strand, within Pf6N2E2_1060 at 1237.717 kb on - strand, within Pf6N2E2_1060 at 1237.717 kb on - strand, within Pf6N2E2_1060 at 1237.717 kb on - strand, within Pf6N2E2_1060 at 1237.717 kb on - strand, within Pf6N2E2_1060 at 1237.717 kb on - strand, within Pf6N2E2_1060 at 1237.717 kb on - strand, within Pf6N2E2_1060 at 1237.717 kb on - strand, within Pf6N2E2_1060 at 1237.786 kb on + strand, within Pf6N2E2_1060 at 1237.786 kb on + strand, within Pf6N2E2_1060 at 1237.786 kb on + strand, within Pf6N2E2_1060 at 1237.786 kb on + strand, within Pf6N2E2_1060 at 1237.786 kb on + strand, within Pf6N2E2_1060 at 1237.786 kb on + strand, within Pf6N2E2_1060 at 1237.786 kb on + strand, within Pf6N2E2_1060 at 1237.786 kb on + strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.900 kb on + strand, within Pf6N2E2_1060 at 1237.991 kb on + strand, within Pf6N2E2_1061 at 1238.031 kb on + strand, within Pf6N2E2_1061 at 1238.131 kb on + strand at 1238.186 kb on + strand at 1238.186 kb on + strand at 1238.187 kb on - strand at 1238.259 kb on + strand at 1238.260 kb on - strand at 1238.390 kb on - strand at 1238.390 kb on - strand at 1238.405 kb on + strand at 1238.405 kb on + strand at 1238.411 kb on + strand at 1238.411 kb on + strand at 1238.411 kb on + strand at 1238.411 kb on + strand at 1238.411 kb on + strand at 1238.412 kb on - strand at 1238.824 kb on + strand, within Pf6N2E2_1062 at 1238.824 kb on + strand, within Pf6N2E2_1062 at 1238.825 kb on - strand, within Pf6N2E2_1062 at 1238.825 kb on - strand, within Pf6N2E2_1062
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (N) remove 1,236,443 + Pf6N2E2_1059 0.59 +0.2 1,236,443 + Pf6N2E2_1059 0.59 +0.3 1,236,443 + Pf6N2E2_1059 0.59 +0.9 1,236,444 - Pf6N2E2_1059 0.59 -0.3 1,236,465 - Pf6N2E2_1059 0.61 -0.5 1,236,572 + Pf6N2E2_1059 0.67 -0.8 1,236,573 - Pf6N2E2_1059 0.67 +1.6 1,236,573 - Pf6N2E2_1059 0.67 -0.1 1,236,573 - Pf6N2E2_1059 0.67 -0.2 1,236,913 + Pf6N2E2_1059 0.88 +0.3 1,237,012 + -0.5 1,237,012 + -3.0 1,237,012 + +0.0 1,237,012 + -0.5 1,237,013 - +0.2 1,237,013 - +0.5 1,237,016 + +0.5 1,237,016 + -0.3 1,237,016 + -0.3 1,237,016 + +0.5 1,237,016 + +0.5 1,237,016 + -0.6 1,237,017 - -0.9 1,237,017 - -0.5 1,237,017 - +1.3 1,237,017 - +1.7 1,237,017 - -0.0 1,237,025 + -0.3 1,237,026 - +0.4 1,237,099 + -0.2 1,237,115 + +0.3 1,237,116 - -0.4 1,237,116 - +0.7 1,237,221 - -0.4 1,237,344 + +0.3 1,237,456 + Pf6N2E2_1060 0.24 -1.2 1,237,638 + Pf6N2E2_1060 0.50 -0.8 1,237,638 + Pf6N2E2_1060 0.50 +0.1 1,237,639 - Pf6N2E2_1060 0.50 -0.3 1,237,639 - Pf6N2E2_1060 0.50 -1.8 1,237,675 + Pf6N2E2_1060 0.55 -1.0 1,237,675 + Pf6N2E2_1060 0.55 +0.3 1,237,676 - Pf6N2E2_1060 0.55 +0.1 1,237,676 - Pf6N2E2_1060 0.55 -0.6 1,237,676 - Pf6N2E2_1060 0.55 +1.2 1,237,676 - Pf6N2E2_1060 0.55 -0.7 1,237,676 - Pf6N2E2_1060 0.55 +0.6 1,237,716 + Pf6N2E2_1060 0.61 -0.2 1,237,717 - Pf6N2E2_1060 0.61 -0.2 1,237,717 - Pf6N2E2_1060 0.61 +0.1 1,237,717 - Pf6N2E2_1060 0.61 -1.3 1,237,717 - Pf6N2E2_1060 0.61 +0.1 1,237,717 - Pf6N2E2_1060 0.61 -0.2 1,237,717 - Pf6N2E2_1060 0.61 -1.0 1,237,717 - Pf6N2E2_1060 0.61 +0.2 1,237,717 - Pf6N2E2_1060 0.61 -1.6 1,237,786 + Pf6N2E2_1060 0.71 +1.3 1,237,786 + Pf6N2E2_1060 0.71 -2.6 1,237,786 + Pf6N2E2_1060 0.71 -0.1 1,237,786 + Pf6N2E2_1060 0.71 +0.5 1,237,786 + Pf6N2E2_1060 0.71 +0.2 1,237,786 + Pf6N2E2_1060 0.71 -0.1 1,237,786 + Pf6N2E2_1060 0.71 +1.4 1,237,786 + Pf6N2E2_1060 0.71 -0.3 1,237,787 - Pf6N2E2_1060 0.71 -1.4 1,237,787 - Pf6N2E2_1060 0.71 +0.1 1,237,787 - Pf6N2E2_1060 0.71 -0.3 1,237,787 - Pf6N2E2_1060 0.71 -2.0 1,237,787 - Pf6N2E2_1060 0.71 +0.8 1,237,787 - Pf6N2E2_1060 0.71 -1.7 1,237,787 - Pf6N2E2_1060 0.71 +0.4 1,237,787 - Pf6N2E2_1060 0.71 -0.4 1,237,787 - Pf6N2E2_1060 0.71 -1.8 1,237,787 - Pf6N2E2_1060 0.71 +0.3 1,237,787 - Pf6N2E2_1060 0.71 -1.3 1,237,900 + Pf6N2E2_1060 0.88 -0.8 1,237,991 + Pf6N2E2_1061 0.23 +1.1 1,238,031 + Pf6N2E2_1061 0.54 -1.7 1,238,131 + -1.0 1,238,186 + -0.3 1,238,186 + +0.2 1,238,187 - -2.0 1,238,259 + -1.5 1,238,260 - +1.3 1,238,390 - +1.9 1,238,390 - +1.4 1,238,405 + -0.5 1,238,405 + +1.1 1,238,411 + +0.1 1,238,411 + +0.0 1,238,411 + -1.1 1,238,411 + -0.2 1,238,411 + +1.0 1,238,412 - +0.4 1,238,824 + Pf6N2E2_1062 0.24 +0.7 1,238,824 + Pf6N2E2_1062 0.24 +1.3 1,238,825 - Pf6N2E2_1062 0.24 -0.6 1,238,825 - Pf6N2E2_1062 0.24 +0.3
Or see this region's nucleotide sequence