Experiment: Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_629 and Pf6N2E2_630 overlap by 8 nucleotides Pf6N2E2_630 and Pf6N2E2_631 are separated by 63 nucleotides Pf6N2E2_631 and Pf6N2E2_632 are separated by 287 nucleotides
Pf6N2E2_629 - 2-ketogluconate kinase (EC 2.7.1.13), at 716,617 to 717,591
_629
Pf6N2E2_630 - Epimerase KguE, at 717,584 to 718,366
_630
Pf6N2E2_631 - 2-ketogluconate utilization repressor PtxS, at 718,430 to 719,455
_631
Pf6N2E2_632 - hypothetical protein, at 719,743 to 721,851
_632
Position (kb) 718
719
720 Strain fitness (log2 ratio) -2
-1
0
1 at 717.698 kb on + strand, within Pf6N2E2_630 at 717.699 kb on - strand, within Pf6N2E2_630 at 717.813 kb on + strand, within Pf6N2E2_630 at 717.813 kb on + strand, within Pf6N2E2_630 at 717.813 kb on + strand, within Pf6N2E2_630 at 717.814 kb on - strand, within Pf6N2E2_630 at 717.814 kb on - strand, within Pf6N2E2_630 at 718.071 kb on - strand, within Pf6N2E2_630 at 718.370 kb on + strand at 718.370 kb on + strand at 718.370 kb on + strand at 718.370 kb on + strand at 718.370 kb on + strand at 718.371 kb on - strand at 718.371 kb on - strand at 718.373 kb on + strand at 718.373 kb on + strand at 718.634 kb on + strand, within Pf6N2E2_631 at 718.634 kb on + strand, within Pf6N2E2_631 at 718.635 kb on - strand, within Pf6N2E2_631 at 718.635 kb on - strand, within Pf6N2E2_631 at 719.083 kb on - strand, within Pf6N2E2_631 at 719.083 kb on - strand, within Pf6N2E2_631 at 719.083 kb on - strand, within Pf6N2E2_631 at 719.152 kb on + strand, within Pf6N2E2_631 at 719.152 kb on + strand, within Pf6N2E2_631 at 719.153 kb on - strand, within Pf6N2E2_631 at 719.153 kb on - strand, within Pf6N2E2_631 at 719.153 kb on - strand, within Pf6N2E2_631 at 719.472 kb on - strand at 719.472 kb on - strand at 719.481 kb on - strand at 719.481 kb on - strand at 719.481 kb on - strand at 719.484 kb on - strand at 719.736 kb on - strand at 719.773 kb on + strand at 719.773 kb on + strand at 719.774 kb on - strand at 719.774 kb on - strand at 719.776 kb on + strand at 719.776 kb on + strand at 719.777 kb on - strand at 719.805 kb on - strand at 719.897 kb on - strand at 720.008 kb on - strand, within Pf6N2E2_632 at 720.187 kb on + strand, within Pf6N2E2_632 at 720.188 kb on - strand, within Pf6N2E2_632 at 720.377 kb on + strand, within Pf6N2E2_632
Per-strain Table
Position Strand Gene LocusTag Fraction Glycine (N) remove 717,698 + Pf6N2E2_630 0.15 +0.6 717,699 - Pf6N2E2_630 0.15 -0.2 717,813 + Pf6N2E2_630 0.29 +0.8 717,813 + Pf6N2E2_630 0.29 -0.3 717,813 + Pf6N2E2_630 0.29 +0.6 717,814 - Pf6N2E2_630 0.29 -1.6 717,814 - Pf6N2E2_630 0.29 +0.8 718,071 - Pf6N2E2_630 0.62 -0.1 718,370 + -0.5 718,370 + +0.4 718,370 + -0.5 718,370 + -1.0 718,370 + -0.3 718,371 - -0.8 718,371 - +0.4 718,373 + -0.9 718,373 + +0.5 718,634 + Pf6N2E2_631 0.20 -0.3 718,634 + Pf6N2E2_631 0.20 +0.2 718,635 - Pf6N2E2_631 0.20 -1.5 718,635 - Pf6N2E2_631 0.20 +0.1 719,083 - Pf6N2E2_631 0.64 -2.6 719,083 - Pf6N2E2_631 0.64 +0.4 719,083 - Pf6N2E2_631 0.64 -1.6 719,152 + Pf6N2E2_631 0.70 -0.7 719,152 + Pf6N2E2_631 0.70 -1.2 719,153 - Pf6N2E2_631 0.70 -0.1 719,153 - Pf6N2E2_631 0.70 -0.0 719,153 - Pf6N2E2_631 0.70 -0.4 719,472 - -1.9 719,472 - +1.2 719,481 - +0.9 719,481 - +0.7 719,481 - +0.1 719,484 - -0.5 719,736 - -0.9 719,773 + -0.5 719,773 + +0.1 719,774 - -0.2 719,774 - +0.0 719,776 + -0.2 719,776 + +1.4 719,777 - +0.5 719,805 - +0.7 719,897 - -0.2 720,008 - Pf6N2E2_632 0.13 +1.2 720,187 + Pf6N2E2_632 0.21 +0.9 720,188 - Pf6N2E2_632 0.21 -0.8 720,377 + Pf6N2E2_632 0.30 -0.7
Or see this region's nucleotide sequence