Experiment: BHIS with Oxytetracycline 0.0001953125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0105 and BT0106 are separated by 105 nucleotides BT0106 and BT0107 are separated by 449 nucleotides BT0107 and BT0108 are separated by 55 nucleotides
BT0105: BT0105 - DNA topoisomerase I (Omega-protein) (NCBI ptt file), at 96,886 to 98,967
BT0105
BT0106: BT0106 - conserved hypothetical protein (NCBI ptt file), at 99,073 to 99,519
BT0106
BT0107: BT0107 - conserved hypothetical protein (NCBI ptt file), at 99,969 to 100,247
BT0107
BT0108: BT0108 - conserved hypothetical protein (NCBI ptt file), at 100,303 to 102,303
BT0108
Position (kb) 99
100 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 98.276 kb on + strand, within BT0105 at 98.349 kb on + strand, within BT0105 at 98.452 kb on + strand, within BT0105 at 98.453 kb on - strand, within BT0105 at 98.499 kb on + strand, within BT0105 at 98.565 kb on + strand, within BT0105 at 98.630 kb on - strand, within BT0105 at 98.852 kb on + strand at 98.925 kb on + strand at 99.011 kb on - strand at 99.084 kb on + strand at 99.084 kb on + strand at 99.333 kb on + strand, within BT0106 at 99.374 kb on + strand, within BT0106 at 99.399 kb on + strand, within BT0106 at 99.407 kb on + strand, within BT0106 at 99.443 kb on + strand, within BT0106 at 99.482 kb on + strand at 99.482 kb on + strand at 99.574 kb on - strand at 99.574 kb on - strand at 99.584 kb on - strand at 99.616 kb on + strand at 99.622 kb on + strand at 99.638 kb on - strand at 99.666 kb on - strand at 99.723 kb on - strand at 99.731 kb on - strand at 99.731 kb on - strand at 99.808 kb on - strand at 99.812 kb on + strand at 99.813 kb on - strand at 99.817 kb on - strand at 99.841 kb on + strand at 99.841 kb on + strand at 99.842 kb on - strand at 99.842 kb on - strand at 99.857 kb on + strand at 99.877 kb on - strand at 99.990 kb on + strand at 100.000 kb on - strand, within BT0107 at 100.015 kb on + strand, within BT0107 at 100.026 kb on - strand, within BT0107 at 100.051 kb on - strand, within BT0107 at 100.107 kb on + strand, within BT0107 at 100.108 kb on - strand, within BT0107 at 100.205 kb on + strand, within BT0107 at 100.243 kb on + strand at 100.244 kb on - strand at 100.247 kb on + strand at 100.248 kb on - strand at 100.254 kb on - strand at 100.267 kb on - strand at 100.282 kb on + strand at 100.353 kb on - strand at 100.399 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Oxytetracycline 0.0001953125 mM remove 98,276 + BT0105 0.67 +0.5 98,349 + BT0105 0.70 -0.7 98,452 + BT0105 0.75 -0.9 98,453 - BT0105 0.75 -0.5 98,499 + BT0105 0.77 -2.1 98,565 + BT0105 0.81 +0.5 98,630 - BT0105 0.84 +0.5 98,852 + -0.2 98,925 + -0.5 99,011 - -1.8 99,084 + -0.9 99,084 + +0.5 99,333 + BT0106 0.58 +0.9 99,374 + BT0106 0.67 +0.5 99,399 + BT0106 0.73 +0.5 99,407 + BT0106 0.75 +0.2 99,443 + BT0106 0.83 +1.4 99,482 + +1.1 99,482 + -1.5 99,574 - -1.5 99,574 - -0.7 99,584 - +0.5 99,616 + -1.5 99,622 + -0.5 99,638 - +0.5 99,666 - -1.7 99,723 - -0.1 99,731 - -2.3 99,731 - -2.2 99,808 - -2.1 99,812 + -1.1 99,813 - -1.5 99,817 - -0.1 99,841 + -1.7 99,841 + -1.3 99,842 - +0.5 99,842 - +1.5 99,857 + +0.5 99,877 - -0.5 99,990 + -0.5 100,000 - BT0107 0.11 -1.5 100,015 + BT0107 0.16 -0.5 100,026 - BT0107 0.20 -3.1 100,051 - BT0107 0.29 -3.0 100,107 + BT0107 0.49 +0.2 100,108 - BT0107 0.50 -3.1 100,205 + BT0107 0.85 +1.5 100,243 + -0.5 100,244 - +0.5 100,247 + -1.1 100,248 - -1.1 100,254 - -0.5 100,267 - -2.1 100,282 + -1.1 100,353 - -1.8 100,399 + -2.1
Or see this region's nucleotide sequence