Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_489 and Pf6N2E2_490 are separated by 173 nucleotides Pf6N2E2_490 and Pf6N2E2_491 are separated by 788 nucleotides
Pf6N2E2_489 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 555,397 to 556,218
_489
Pf6N2E2_490 - PhaK-like protein, at 556,392 to 557,693
_490
Pf6N2E2_491 - hypothetical protein, at 558,482 to 558,835
_491
Position (kb) 556
557
558 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 555.440 kb on + strand at 555.584 kb on + strand, within Pf6N2E2_489 at 555.584 kb on + strand, within Pf6N2E2_489 at 555.615 kb on - strand, within Pf6N2E2_489 at 555.747 kb on - strand, within Pf6N2E2_489 at 555.770 kb on + strand, within Pf6N2E2_489 at 555.770 kb on + strand, within Pf6N2E2_489 at 555.771 kb on - strand, within Pf6N2E2_489 at 555.771 kb on - strand, within Pf6N2E2_489 at 555.791 kb on + strand, within Pf6N2E2_489 at 555.791 kb on + strand, within Pf6N2E2_489 at 555.791 kb on + strand, within Pf6N2E2_489 at 555.843 kb on - strand, within Pf6N2E2_489 at 555.911 kb on + strand, within Pf6N2E2_489 at 555.911 kb on + strand, within Pf6N2E2_489 at 555.911 kb on + strand, within Pf6N2E2_489 at 555.911 kb on + strand, within Pf6N2E2_489 at 556.177 kb on + strand at 556.177 kb on + strand at 556.177 kb on + strand at 556.178 kb on - strand at 556.178 kb on - strand at 556.178 kb on - strand at 556.224 kb on + strand at 556.225 kb on - strand at 556.237 kb on + strand at 556.237 kb on + strand at 556.246 kb on + strand at 556.246 kb on + strand at 556.300 kb on + strand at 556.393 kb on + strand at 556.453 kb on + strand at 556.453 kb on + strand at 556.516 kb on - strand at 557.014 kb on + strand, within Pf6N2E2_490 at 557.014 kb on + strand, within Pf6N2E2_490 at 557.380 kb on + strand, within Pf6N2E2_490 at 557.380 kb on + strand, within Pf6N2E2_490 at 557.538 kb on - strand, within Pf6N2E2_490 at 557.694 kb on + strand at 557.694 kb on + strand at 557.759 kb on + strand at 557.803 kb on + strand at 557.803 kb on + strand at 557.949 kb on + strand at 557.968 kb on + strand at 557.968 kb on + strand at 558.118 kb on - strand at 558.154 kb on + strand at 558.154 kb on + strand at 558.154 kb on + strand at 558.154 kb on + strand at 558.155 kb on - strand at 558.155 kb on - strand at 558.175 kb on + strand at 558.175 kb on + strand at 558.176 kb on - strand at 558.176 kb on - strand at 558.176 kb on - strand at 558.176 kb on - strand at 558.197 kb on + strand at 558.197 kb on + strand at 558.198 kb on - strand at 558.198 kb on - strand at 558.198 kb on - strand at 558.231 kb on + strand at 558.232 kb on - strand at 558.245 kb on + strand at 558.246 kb on - strand at 558.246 kb on - strand at 558.306 kb on - strand at 558.397 kb on + strand at 558.397 kb on + strand at 558.409 kb on + strand at 558.410 kb on - strand at 558.423 kb on + strand at 558.424 kb on - strand at 558.424 kb on - strand at 558.458 kb on + strand at 558.458 kb on + strand at 558.458 kb on + strand at 558.459 kb on - strand at 558.459 kb on - strand at 558.459 kb on - strand at 558.461 kb on + strand at 558.461 kb on + strand at 558.461 kb on + strand at 558.462 kb on - strand at 558.462 kb on - strand at 558.462 kb on - strand at 558.462 kb on - strand at 558.462 kb on - strand at 558.481 kb on + strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.482 kb on - strand at 558.578 kb on + strand, within Pf6N2E2_491 at 558.578 kb on + strand, within Pf6N2E2_491 at 558.579 kb on - strand, within Pf6N2E2_491 at 558.579 kb on - strand, within Pf6N2E2_491 at 558.579 kb on - strand, within Pf6N2E2_491 at 558.581 kb on + strand, within Pf6N2E2_491 at 558.581 kb on + strand, within Pf6N2E2_491 at 558.582 kb on - strand, within Pf6N2E2_491 at 558.582 kb on - strand, within Pf6N2E2_491 at 558.582 kb on - strand, within Pf6N2E2_491
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 555,440 + +0.7 555,584 + Pf6N2E2_489 0.23 +0.0 555,584 + Pf6N2E2_489 0.23 -1.6 555,615 - Pf6N2E2_489 0.27 +0.3 555,747 - Pf6N2E2_489 0.43 -0.7 555,770 + Pf6N2E2_489 0.45 -0.6 555,770 + Pf6N2E2_489 0.45 +0.0 555,771 - Pf6N2E2_489 0.45 +0.2 555,771 - Pf6N2E2_489 0.45 -0.1 555,791 + Pf6N2E2_489 0.48 -0.0 555,791 + Pf6N2E2_489 0.48 +0.8 555,791 + Pf6N2E2_489 0.48 -1.1 555,843 - Pf6N2E2_489 0.54 -0.1 555,911 + Pf6N2E2_489 0.63 +0.8 555,911 + Pf6N2E2_489 0.63 -1.5 555,911 + Pf6N2E2_489 0.63 -0.3 555,911 + Pf6N2E2_489 0.63 +1.9 556,177 + +1.8 556,177 + +1.1 556,177 + -0.3 556,178 - -0.9 556,178 - -0.2 556,178 - -0.4 556,224 + -0.4 556,225 - +0.4 556,237 + +1.2 556,237 + +1.0 556,246 + -0.1 556,246 + -1.1 556,300 + -0.6 556,393 + +0.2 556,453 + +0.7 556,453 + -0.5 556,516 - +0.9 557,014 + Pf6N2E2_490 0.48 -1.5 557,014 + Pf6N2E2_490 0.48 -0.6 557,380 + Pf6N2E2_490 0.76 -1.1 557,380 + Pf6N2E2_490 0.76 -0.2 557,538 - Pf6N2E2_490 0.88 -0.1 557,694 + -0.2 557,694 + +0.6 557,759 + -0.1 557,803 + -0.9 557,803 + +0.9 557,949 + +0.5 557,968 + +1.1 557,968 + +0.3 558,118 - +0.5 558,154 + +0.0 558,154 + -0.9 558,154 + +0.0 558,154 + -0.2 558,155 - -2.8 558,155 - -0.3 558,175 + +0.7 558,175 + -1.5 558,176 - -0.1 558,176 - +1.3 558,176 - -1.6 558,176 - +0.3 558,197 + +0.9 558,197 + -0.1 558,198 - -0.5 558,198 - +0.4 558,198 - +1.4 558,231 + +1.0 558,232 - +0.3 558,245 + +0.0 558,246 - +1.6 558,246 - +1.1 558,306 - -1.4 558,397 + -0.9 558,397 + +0.5 558,409 + +0.1 558,410 - -0.6 558,423 + -0.4 558,424 - -0.2 558,424 - +1.3 558,458 + -0.7 558,458 + +0.3 558,458 + +0.2 558,459 - -0.6 558,459 - +1.0 558,459 - +1.0 558,461 + +0.1 558,461 + -0.8 558,461 + +1.2 558,462 - -0.2 558,462 - +0.2 558,462 - +2.3 558,462 - -1.9 558,462 - +0.7 558,481 + +0.9 558,482 - +0.2 558,482 - +0.3 558,482 - +0.4 558,482 - -0.4 558,482 - -0.5 558,482 - +0.5 558,482 - -0.1 558,482 - +0.6 558,482 - +0.8 558,482 - -0.2 558,482 - +0.6 558,578 + Pf6N2E2_491 0.27 -0.1 558,578 + Pf6N2E2_491 0.27 -0.3 558,579 - Pf6N2E2_491 0.27 +0.9 558,579 - Pf6N2E2_491 0.27 -0.3 558,579 - Pf6N2E2_491 0.27 -0.5 558,581 + Pf6N2E2_491 0.28 -0.6 558,581 + Pf6N2E2_491 0.28 +0.5 558,582 - Pf6N2E2_491 0.28 +0.5 558,582 - Pf6N2E2_491 0.28 +1.1 558,582 - Pf6N2E2_491 0.28 +0.8
Or see this region's nucleotide sequence