Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_450 and Pf6N2E2_451 are separated by 110 nucleotides Pf6N2E2_451 and Pf6N2E2_452 are separated by 211 nucleotides Pf6N2E2_452 and Pf6N2E2_453 are separated by 105 nucleotides
Pf6N2E2_450 - FIG00953950: hypothetical protein, at 512,754 to 513,281
_450
Pf6N2E2_451 - transcriptional regulator MvaT, P16 subunit, putative, at 513,392 to 513,757
_451
Pf6N2E2_452 - Rtn protein, at 513,969 to 515,513
_452
Pf6N2E2_453 - LysR family transcriptional regulator STM2281, at 515,619 to 516,482
_453
Position (kb)
513
514
515
516 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 513.125 kb on + strand, within Pf6N2E2_450 at 513.125 kb on + strand, within Pf6N2E2_450 at 513.151 kb on + strand, within Pf6N2E2_450 at 513.151 kb on + strand, within Pf6N2E2_450 at 513.151 kb on + strand, within Pf6N2E2_450 at 513.151 kb on + strand, within Pf6N2E2_450 at 513.151 kb on + strand, within Pf6N2E2_450 at 513.151 kb on + strand, within Pf6N2E2_450 at 513.151 kb on + strand, within Pf6N2E2_450 at 513.151 kb on + strand, within Pf6N2E2_450 at 513.152 kb on - strand, within Pf6N2E2_450 at 513.152 kb on - strand, within Pf6N2E2_450 at 513.152 kb on - strand at 513.152 kb on - strand, within Pf6N2E2_450 at 513.152 kb on - strand, within Pf6N2E2_450 at 513.152 kb on - strand, within Pf6N2E2_450 at 513.152 kb on - strand, within Pf6N2E2_450 at 513.178 kb on + strand, within Pf6N2E2_450 at 513.179 kb on - strand, within Pf6N2E2_450 at 513.179 kb on - strand, within Pf6N2E2_450 at 513.195 kb on + strand, within Pf6N2E2_450 at 513.195 kb on + strand, within Pf6N2E2_450 at 513.195 kb on + strand, within Pf6N2E2_450 at 513.195 kb on + strand, within Pf6N2E2_450 at 513.195 kb on + strand, within Pf6N2E2_450 at 513.195 kb on + strand, within Pf6N2E2_450 at 513.196 kb on - strand, within Pf6N2E2_450 at 513.196 kb on - strand, within Pf6N2E2_450 at 513.196 kb on - strand, within Pf6N2E2_450 at 513.196 kb on - strand, within Pf6N2E2_450 at 513.196 kb on - strand, within Pf6N2E2_450 at 513.196 kb on - strand, within Pf6N2E2_450 at 513.196 kb on - strand, within Pf6N2E2_450 at 513.196 kb on - strand, within Pf6N2E2_450 at 513.208 kb on - strand, within Pf6N2E2_450 at 513.208 kb on - strand, within Pf6N2E2_450 at 513.250 kb on + strand at 513.251 kb on - strand at 513.278 kb on - strand at 513.296 kb on + strand at 513.430 kb on - strand, within Pf6N2E2_451 at 513.430 kb on - strand, within Pf6N2E2_451 at 513.516 kb on - strand, within Pf6N2E2_451 at 513.779 kb on - strand at 513.779 kb on - strand at 513.811 kb on - strand at 513.811 kb on - strand at 513.846 kb on - strand at 513.859 kb on - strand at 513.873 kb on - strand at 513.873 kb on - strand at 513.914 kb on - strand at 513.932 kb on + strand at 513.955 kb on + strand at 513.980 kb on + strand at 514.032 kb on + strand at 514.032 kb on + strand at 514.032 kb on + strand at 514.032 kb on + strand at 514.033 kb on - strand at 514.036 kb on + strand at 514.037 kb on - strand at 514.045 kb on - strand at 514.240 kb on + strand, within Pf6N2E2_452 at 514.240 kb on + strand, within Pf6N2E2_452 at 514.241 kb on - strand, within Pf6N2E2_452 at 514.335 kb on - strand, within Pf6N2E2_452 at 514.335 kb on - strand, within Pf6N2E2_452 at 514.383 kb on - strand, within Pf6N2E2_452 at 514.549 kb on - strand, within Pf6N2E2_452 at 514.668 kb on + strand, within Pf6N2E2_452 at 514.685 kb on - strand, within Pf6N2E2_452 at 514.685 kb on - strand, within Pf6N2E2_452 at 514.685 kb on - strand, within Pf6N2E2_452 at 514.831 kb on + strand, within Pf6N2E2_452 at 514.831 kb on + strand, within Pf6N2E2_452 at 514.866 kb on + strand, within Pf6N2E2_452 at 514.866 kb on + strand, within Pf6N2E2_452 at 514.867 kb on - strand, within Pf6N2E2_452 at 514.867 kb on - strand, within Pf6N2E2_452 at 515.194 kb on + strand, within Pf6N2E2_452 at 515.195 kb on - strand, within Pf6N2E2_452 at 515.195 kb on - strand, within Pf6N2E2_452 at 515.195 kb on - strand, within Pf6N2E2_452 at 515.195 kb on - strand, within Pf6N2E2_452 at 515.195 kb on - strand, within Pf6N2E2_452 at 515.195 kb on - strand, within Pf6N2E2_452 at 515.213 kb on + strand, within Pf6N2E2_452 at 515.214 kb on - strand, within Pf6N2E2_452 at 515.214 kb on - strand, within Pf6N2E2_452 at 515.214 kb on - strand, within Pf6N2E2_452 at 515.214 kb on - strand, within Pf6N2E2_452 at 515.304 kb on + strand, within Pf6N2E2_452 at 515.304 kb on + strand, within Pf6N2E2_452 at 515.304 kb on + strand, within Pf6N2E2_452 at 515.304 kb on + strand, within Pf6N2E2_452 at 515.304 kb on + strand, within Pf6N2E2_452 at 515.304 kb on + strand, within Pf6N2E2_452 at 515.304 kb on + strand, within Pf6N2E2_452 at 515.304 kb on + strand at 515.305 kb on - strand, within Pf6N2E2_452 at 515.305 kb on - strand, within Pf6N2E2_452 at 515.305 kb on - strand, within Pf6N2E2_452 at 515.305 kb on - strand, within Pf6N2E2_452 at 515.305 kb on - strand, within Pf6N2E2_452 at 515.305 kb on - strand, within Pf6N2E2_452 at 515.305 kb on - strand, within Pf6N2E2_452 at 515.305 kb on - strand, within Pf6N2E2_452 at 515.413 kb on + strand at 515.413 kb on + strand at 515.414 kb on - strand at 515.442 kb on + strand at 515.569 kb on + strand at 515.569 kb on + strand at 515.569 kb on + strand at 515.569 kb on + strand at 515.569 kb on + strand at 515.569 kb on + strand at 515.570 kb on - strand at 515.570 kb on - strand at 515.972 kb on + strand, within Pf6N2E2_453 at 515.973 kb on - strand, within Pf6N2E2_453 at 516.017 kb on + strand, within Pf6N2E2_453 at 516.508 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 513,125 + Pf6N2E2_450 0.70 +0.3 513,125 + Pf6N2E2_450 0.70 -0.5 513,151 + Pf6N2E2_450 0.75 +0.2 513,151 + Pf6N2E2_450 0.75 -0.5 513,151 + Pf6N2E2_450 0.75 +0.1 513,151 + Pf6N2E2_450 0.75 +1.1 513,151 + Pf6N2E2_450 0.75 -0.9 513,151 + Pf6N2E2_450 0.75 +0.6 513,151 + Pf6N2E2_450 0.75 -0.4 513,151 + Pf6N2E2_450 0.75 +0.3 513,152 - Pf6N2E2_450 0.75 -0.7 513,152 - Pf6N2E2_450 0.75 +0.6 513,152 - +2.0 513,152 - Pf6N2E2_450 0.75 -0.2 513,152 - Pf6N2E2_450 0.75 +0.1 513,152 - Pf6N2E2_450 0.75 -0.0 513,152 - Pf6N2E2_450 0.75 +1.4 513,178 + Pf6N2E2_450 0.80 +0.3 513,179 - Pf6N2E2_450 0.80 +0.0 513,179 - Pf6N2E2_450 0.80 +0.7 513,195 + Pf6N2E2_450 0.84 +0.5 513,195 + Pf6N2E2_450 0.84 -1.4 513,195 + Pf6N2E2_450 0.84 -0.2 513,195 + Pf6N2E2_450 0.84 +0.3 513,195 + Pf6N2E2_450 0.84 -0.0 513,195 + Pf6N2E2_450 0.84 +0.3 513,196 - Pf6N2E2_450 0.84 -1.0 513,196 - Pf6N2E2_450 0.84 +0.2 513,196 - Pf6N2E2_450 0.84 -1.0 513,196 - Pf6N2E2_450 0.84 +0.3 513,196 - Pf6N2E2_450 0.84 -0.2 513,196 - Pf6N2E2_450 0.84 -0.2 513,196 - Pf6N2E2_450 0.84 -2.4 513,196 - Pf6N2E2_450 0.84 -0.6 513,208 - Pf6N2E2_450 0.86 +0.3 513,208 - Pf6N2E2_450 0.86 -0.0 513,250 + +0.7 513,251 - +0.5 513,278 - -0.3 513,296 + +0.6 513,430 - Pf6N2E2_451 0.10 -0.3 513,430 - Pf6N2E2_451 0.10 +0.3 513,516 - Pf6N2E2_451 0.34 -2.6 513,779 - +0.1 513,779 - -1.4 513,811 - +2.0 513,811 - +0.7 513,846 - +0.3 513,859 - -1.0 513,873 - -0.2 513,873 - +0.3 513,914 - -2.2 513,932 + +1.7 513,955 + -0.0 513,980 + -0.2 514,032 + +0.6 514,032 + -0.6 514,032 + -1.9 514,032 + +0.2 514,033 - +0.4 514,036 + +0.6 514,037 - -1.0 514,045 - -0.3 514,240 + Pf6N2E2_452 0.18 -0.6 514,240 + Pf6N2E2_452 0.18 -1.4 514,241 - Pf6N2E2_452 0.18 +1.2 514,335 - Pf6N2E2_452 0.24 -0.5 514,335 - Pf6N2E2_452 0.24 -0.5 514,383 - Pf6N2E2_452 0.27 -0.5 514,549 - Pf6N2E2_452 0.38 -0.5 514,668 + Pf6N2E2_452 0.45 -1.1 514,685 - Pf6N2E2_452 0.46 -0.9 514,685 - Pf6N2E2_452 0.46 +1.2 514,685 - Pf6N2E2_452 0.46 -1.1 514,831 + Pf6N2E2_452 0.56 -1.4 514,831 + Pf6N2E2_452 0.56 -0.7 514,866 + Pf6N2E2_452 0.58 -0.9 514,866 + Pf6N2E2_452 0.58 +0.3 514,867 - Pf6N2E2_452 0.58 +0.2 514,867 - Pf6N2E2_452 0.58 +0.8 515,194 + Pf6N2E2_452 0.79 -0.9 515,195 - Pf6N2E2_452 0.79 -0.5 515,195 - Pf6N2E2_452 0.79 -2.0 515,195 - Pf6N2E2_452 0.79 +1.0 515,195 - Pf6N2E2_452 0.79 -0.0 515,195 - Pf6N2E2_452 0.79 -0.2 515,195 - Pf6N2E2_452 0.79 -0.5 515,213 + Pf6N2E2_452 0.81 +1.3 515,214 - Pf6N2E2_452 0.81 +0.5 515,214 - Pf6N2E2_452 0.81 +0.5 515,214 - Pf6N2E2_452 0.81 -0.2 515,214 - Pf6N2E2_452 0.81 +0.4 515,304 + Pf6N2E2_452 0.86 -0.5 515,304 + Pf6N2E2_452 0.86 +0.4 515,304 + Pf6N2E2_452 0.86 -0.9 515,304 + Pf6N2E2_452 0.86 +0.8 515,304 + Pf6N2E2_452 0.86 -1.0 515,304 + Pf6N2E2_452 0.86 -1.1 515,304 + Pf6N2E2_452 0.86 -0.9 515,304 + -0.6 515,305 - Pf6N2E2_452 0.86 +0.1 515,305 - Pf6N2E2_452 0.86 +0.4 515,305 - Pf6N2E2_452 0.86 -0.5 515,305 - Pf6N2E2_452 0.86 -0.7 515,305 - Pf6N2E2_452 0.86 -2.0 515,305 - Pf6N2E2_452 0.86 -0.7 515,305 - Pf6N2E2_452 0.86 +0.6 515,305 - Pf6N2E2_452 0.86 -0.3 515,413 + -0.4 515,413 + -0.7 515,414 - -0.5 515,442 + -0.1 515,569 + +1.4 515,569 + +1.4 515,569 + -0.5 515,569 + +0.1 515,569 + -1.0 515,569 + -0.4 515,570 - +0.8 515,570 - +0.3 515,972 + Pf6N2E2_453 0.41 +0.2 515,973 - Pf6N2E2_453 0.41 -0.4 516,017 + Pf6N2E2_453 0.46 -0.3 516,508 + -0.0
Or see this region's nucleotide sequence