Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2369 and Pf6N2E2_2370 are separated by 59 nucleotides Pf6N2E2_2370 and Pf6N2E2_2371 overlap by 4 nucleotides
Pf6N2E2_2369 - Flagellum-specific ATP synthase FliI, at 2,802,306 to 2,803,556
_2369
Pf6N2E2_2370 - Type III secretion inner membrane channel protein (LcrD,HrcV,EscV,SsaV), at 2,803,616 to 2,805,673
_2370
Pf6N2E2_2371 - Type III secretion outermembrane contact sensing protein (YopN,Yop4b,LcrE), at 2,805,670 to 2,806,773
_2371
Position (kb)
2803
2804
2805
2806 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2802.616 kb on + strand, within Pf6N2E2_2369 at 2802.617 kb on - strand, within Pf6N2E2_2369 at 2802.694 kb on + strand, within Pf6N2E2_2369 at 2802.695 kb on - strand, within Pf6N2E2_2369 at 2802.934 kb on - strand, within Pf6N2E2_2369 at 2803.158 kb on + strand, within Pf6N2E2_2369 at 2803.159 kb on - strand, within Pf6N2E2_2369 at 2803.533 kb on + strand at 2803.533 kb on + strand at 2803.534 kb on - strand at 2803.590 kb on - strand at 2803.590 kb on - strand at 2803.944 kb on + strand, within Pf6N2E2_2370 at 2804.112 kb on + strand, within Pf6N2E2_2370 at 2804.260 kb on - strand, within Pf6N2E2_2370 at 2804.505 kb on + strand, within Pf6N2E2_2370 at 2804.729 kb on + strand, within Pf6N2E2_2370 at 2804.730 kb on - strand, within Pf6N2E2_2370 at 2804.980 kb on - strand, within Pf6N2E2_2370 at 2805.059 kb on - strand, within Pf6N2E2_2370 at 2805.059 kb on - strand, within Pf6N2E2_2370 at 2805.120 kb on + strand, within Pf6N2E2_2370 at 2805.121 kb on - strand, within Pf6N2E2_2370 at 2805.499 kb on - strand at 2805.499 kb on - strand at 2805.717 kb on - strand at 2805.908 kb on + strand, within Pf6N2E2_2371 at 2806.119 kb on + strand, within Pf6N2E2_2371 at 2806.120 kb on - strand, within Pf6N2E2_2371
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 2,802,616 + Pf6N2E2_2369 0.25 -2.8 2,802,617 - Pf6N2E2_2369 0.25 -0.3 2,802,694 + Pf6N2E2_2369 0.31 +0.5 2,802,695 - Pf6N2E2_2369 0.31 +0.3 2,802,934 - Pf6N2E2_2369 0.50 -1.3 2,803,158 + Pf6N2E2_2369 0.68 -1.1 2,803,159 - Pf6N2E2_2369 0.68 -1.4 2,803,533 + -0.4 2,803,533 + -1.6 2,803,534 - +0.0 2,803,590 - +1.6 2,803,590 - -0.4 2,803,944 + Pf6N2E2_2370 0.16 -0.4 2,804,112 + Pf6N2E2_2370 0.24 +0.6 2,804,260 - Pf6N2E2_2370 0.31 +1.0 2,804,505 + Pf6N2E2_2370 0.43 +1.3 2,804,729 + Pf6N2E2_2370 0.54 +1.3 2,804,730 - Pf6N2E2_2370 0.54 +0.5 2,804,980 - Pf6N2E2_2370 0.66 -1.2 2,805,059 - Pf6N2E2_2370 0.70 -0.0 2,805,059 - Pf6N2E2_2370 0.70 -1.3 2,805,120 + Pf6N2E2_2370 0.73 +1.6 2,805,121 - Pf6N2E2_2370 0.73 +0.8 2,805,499 - +0.2 2,805,499 - -1.1 2,805,717 - +0.2 2,805,908 + Pf6N2E2_2371 0.22 -0.8 2,806,119 + Pf6N2E2_2371 0.41 +0.3 2,806,120 - Pf6N2E2_2371 0.41 -0.3
Or see this region's nucleotide sequence