Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_124 and Pf6N2E2_125 overlap by 4 nucleotides Pf6N2E2_125 and Pf6N2E2_126 are separated by 376 nucleotides
Pf6N2E2_124 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 171,231 to 172,310
_124
Pf6N2E2_125 - UDP-glucose dehydrogenase (EC 1.1.1.22), at 172,307 to 173,671
_125
Pf6N2E2_126 - FIG00960892: hypothetical protein, at 174,048 to 175,745
_126
Position (kb)
172
173
174 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 171.313 kb on + strand at 171.313 kb on + strand at 171.493 kb on + strand, within Pf6N2E2_124 at 171.493 kb on + strand, within Pf6N2E2_124 at 171.493 kb on + strand, within Pf6N2E2_124 at 171.532 kb on + strand, within Pf6N2E2_124 at 171.533 kb on - strand, within Pf6N2E2_124 at 171.649 kb on + strand, within Pf6N2E2_124 at 171.649 kb on + strand, within Pf6N2E2_124 at 171.811 kb on + strand, within Pf6N2E2_124 at 171.811 kb on + strand, within Pf6N2E2_124 at 171.812 kb on - strand, within Pf6N2E2_124 at 171.812 kb on - strand, within Pf6N2E2_124 at 171.812 kb on - strand, within Pf6N2E2_124 at 171.812 kb on - strand, within Pf6N2E2_124 at 171.812 kb on - strand, within Pf6N2E2_124 at 171.916 kb on + strand, within Pf6N2E2_124 at 171.916 kb on + strand, within Pf6N2E2_124 at 171.917 kb on - strand, within Pf6N2E2_124 at 171.937 kb on + strand, within Pf6N2E2_124 at 171.937 kb on + strand, within Pf6N2E2_124 at 171.937 kb on + strand, within Pf6N2E2_124 at 171.937 kb on + strand, within Pf6N2E2_124 at 171.937 kb on + strand, within Pf6N2E2_124 at 171.937 kb on + strand, within Pf6N2E2_124 at 171.981 kb on - strand, within Pf6N2E2_124 at 171.981 kb on - strand, within Pf6N2E2_124 at 171.981 kb on - strand, within Pf6N2E2_124 at 171.981 kb on - strand, within Pf6N2E2_124 at 171.981 kb on - strand, within Pf6N2E2_124 at 172.012 kb on + strand, within Pf6N2E2_124 at 172.013 kb on - strand, within Pf6N2E2_124 at 172.013 kb on - strand, within Pf6N2E2_124 at 172.013 kb on - strand, within Pf6N2E2_124 at 172.013 kb on - strand, within Pf6N2E2_124 at 172.013 kb on - strand, within Pf6N2E2_124 at 172.071 kb on + strand, within Pf6N2E2_124 at 172.202 kb on - strand, within Pf6N2E2_124 at 172.204 kb on + strand at 172.404 kb on + strand at 172.405 kb on - strand at 172.405 kb on - strand at 172.455 kb on - strand, within Pf6N2E2_125 at 172.569 kb on + strand, within Pf6N2E2_125 at 172.605 kb on + strand, within Pf6N2E2_125 at 172.768 kb on + strand, within Pf6N2E2_125 at 172.899 kb on + strand, within Pf6N2E2_125 at 173.106 kb on + strand, within Pf6N2E2_125 at 173.106 kb on + strand, within Pf6N2E2_125 at 173.228 kb on + strand, within Pf6N2E2_125 at 173.644 kb on - strand at 173.644 kb on - strand at 173.644 kb on - strand at 173.657 kb on + strand at 173.657 kb on + strand at 173.657 kb on + strand at 173.657 kb on + strand at 173.701 kb on + strand at 173.702 kb on - strand at 173.720 kb on + strand at 174.084 kb on + strand at 174.105 kb on + strand at 174.108 kb on + strand at 174.108 kb on + strand at 174.109 kb on - strand at 174.204 kb on + strand at 174.204 kb on + strand at 174.205 kb on - strand at 174.209 kb on - strand at 174.209 kb on - strand at 174.209 kb on - strand at 174.209 kb on - strand at 174.209 kb on - strand at 174.209 kb on - strand at 174.276 kb on + strand, within Pf6N2E2_126 at 174.276 kb on + strand, within Pf6N2E2_126 at 174.276 kb on + strand, within Pf6N2E2_126 at 174.276 kb on + strand, within Pf6N2E2_126 at 174.277 kb on - strand, within Pf6N2E2_126 at 174.277 kb on - strand, within Pf6N2E2_126 at 174.298 kb on - strand, within Pf6N2E2_126 at 174.298 kb on - strand, within Pf6N2E2_126 at 174.306 kb on + strand, within Pf6N2E2_126 at 174.306 kb on + strand, within Pf6N2E2_126 at 174.306 kb on + strand, within Pf6N2E2_126 at 174.306 kb on + strand, within Pf6N2E2_126 at 174.306 kb on + strand, within Pf6N2E2_126 at 174.307 kb on - strand, within Pf6N2E2_126 at 174.307 kb on - strand, within Pf6N2E2_126 at 174.307 kb on - strand, within Pf6N2E2_126 at 174.307 kb on - strand, within Pf6N2E2_126 at 174.308 kb on + strand, within Pf6N2E2_126 at 174.309 kb on - strand, within Pf6N2E2_126 at 174.330 kb on + strand, within Pf6N2E2_126 at 174.344 kb on + strand, within Pf6N2E2_126 at 174.360 kb on + strand, within Pf6N2E2_126 at 174.361 kb on - strand, within Pf6N2E2_126 at 174.412 kb on + strand, within Pf6N2E2_126 at 174.413 kb on - strand, within Pf6N2E2_126 at 174.460 kb on - strand, within Pf6N2E2_126 at 174.460 kb on - strand, within Pf6N2E2_126 at 174.555 kb on + strand, within Pf6N2E2_126 at 174.555 kb on + strand, within Pf6N2E2_126 at 174.555 kb on + strand, within Pf6N2E2_126 at 174.555 kb on + strand, within Pf6N2E2_126 at 174.556 kb on - strand, within Pf6N2E2_126 at 174.556 kb on - strand, within Pf6N2E2_126
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 171,313 + +0.2 171,313 + +1.4 171,493 + Pf6N2E2_124 0.24 +0.0 171,493 + Pf6N2E2_124 0.24 -0.0 171,493 + Pf6N2E2_124 0.24 -1.2 171,532 + Pf6N2E2_124 0.28 +0.8 171,533 - Pf6N2E2_124 0.28 -0.9 171,649 + Pf6N2E2_124 0.39 -0.9 171,649 + Pf6N2E2_124 0.39 +0.7 171,811 + Pf6N2E2_124 0.54 -0.7 171,811 + Pf6N2E2_124 0.54 -0.2 171,812 - Pf6N2E2_124 0.54 +0.3 171,812 - Pf6N2E2_124 0.54 +1.3 171,812 - Pf6N2E2_124 0.54 +0.7 171,812 - Pf6N2E2_124 0.54 +0.5 171,812 - Pf6N2E2_124 0.54 -0.7 171,916 + Pf6N2E2_124 0.63 +0.1 171,916 + Pf6N2E2_124 0.63 -0.3 171,917 - Pf6N2E2_124 0.64 +1.1 171,937 + Pf6N2E2_124 0.65 +0.9 171,937 + Pf6N2E2_124 0.65 +0.4 171,937 + Pf6N2E2_124 0.65 -0.5 171,937 + Pf6N2E2_124 0.65 +1.7 171,937 + Pf6N2E2_124 0.65 -0.1 171,937 + Pf6N2E2_124 0.65 -2.4 171,981 - Pf6N2E2_124 0.69 -0.1 171,981 - Pf6N2E2_124 0.69 -0.0 171,981 - Pf6N2E2_124 0.69 +0.9 171,981 - Pf6N2E2_124 0.69 -0.4 171,981 - Pf6N2E2_124 0.69 +0.1 172,012 + Pf6N2E2_124 0.72 +0.3 172,013 - Pf6N2E2_124 0.72 -0.7 172,013 - Pf6N2E2_124 0.72 -0.1 172,013 - Pf6N2E2_124 0.72 -0.4 172,013 - Pf6N2E2_124 0.72 -0.2 172,013 - Pf6N2E2_124 0.72 -0.6 172,071 + Pf6N2E2_124 0.78 -0.1 172,202 - Pf6N2E2_124 0.90 +0.7 172,204 + +0.8 172,404 + +1.1 172,405 - -0.1 172,405 - +0.1 172,455 - Pf6N2E2_125 0.11 -3.1 172,569 + Pf6N2E2_125 0.19 +0.5 172,605 + Pf6N2E2_125 0.22 -0.8 172,768 + Pf6N2E2_125 0.34 -0.9 172,899 + Pf6N2E2_125 0.43 +1.0 173,106 + Pf6N2E2_125 0.59 -1.6 173,106 + Pf6N2E2_125 0.59 -0.3 173,228 + Pf6N2E2_125 0.67 -0.7 173,644 - -1.1 173,644 - -1.0 173,644 - +0.5 173,657 + -0.0 173,657 + -1.5 173,657 + +0.1 173,657 + +1.3 173,701 + +0.2 173,702 - -0.0 173,720 + -0.6 174,084 + -0.0 174,105 + -0.9 174,108 + -1.0 174,108 + -0.5 174,109 - +0.0 174,204 + -0.1 174,204 + +0.7 174,205 - +0.2 174,209 - +0.3 174,209 - +1.0 174,209 - -0.0 174,209 - +0.1 174,209 - -2.0 174,209 - -0.5 174,276 + Pf6N2E2_126 0.13 +1.0 174,276 + Pf6N2E2_126 0.13 -0.5 174,276 + Pf6N2E2_126 0.13 -0.5 174,276 + Pf6N2E2_126 0.13 -0.8 174,277 - Pf6N2E2_126 0.13 -1.2 174,277 - Pf6N2E2_126 0.13 +2.3 174,298 - Pf6N2E2_126 0.15 +0.3 174,298 - Pf6N2E2_126 0.15 -0.5 174,306 + Pf6N2E2_126 0.15 +1.7 174,306 + Pf6N2E2_126 0.15 +0.1 174,306 + Pf6N2E2_126 0.15 -0.5 174,306 + Pf6N2E2_126 0.15 +0.2 174,306 + Pf6N2E2_126 0.15 -0.7 174,307 - Pf6N2E2_126 0.15 -0.4 174,307 - Pf6N2E2_126 0.15 +0.6 174,307 - Pf6N2E2_126 0.15 -0.9 174,307 - Pf6N2E2_126 0.15 -0.3 174,308 + Pf6N2E2_126 0.15 -0.7 174,309 - Pf6N2E2_126 0.15 -0.9 174,330 + Pf6N2E2_126 0.17 +0.3 174,344 + Pf6N2E2_126 0.17 +0.7 174,360 + Pf6N2E2_126 0.18 -0.5 174,361 - Pf6N2E2_126 0.18 +0.4 174,412 + Pf6N2E2_126 0.21 -0.3 174,413 - Pf6N2E2_126 0.21 +2.1 174,460 - Pf6N2E2_126 0.24 +1.0 174,460 - Pf6N2E2_126 0.24 -0.6 174,555 + Pf6N2E2_126 0.30 -0.7 174,555 + Pf6N2E2_126 0.30 -0.2 174,555 + Pf6N2E2_126 0.30 +0.6 174,555 + Pf6N2E2_126 0.30 +1.6 174,556 - Pf6N2E2_126 0.30 -2.0 174,556 - Pf6N2E2_126 0.30 +0.3
Or see this region's nucleotide sequence