Experiment: BHIS with Thioridazine 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4615 and BT4616 are separated by 203 nucleotides BT4616 and BT4617 overlap by 4 nucleotides BT4617 and BT4618 are separated by 314 nucleotides BT4618 and BT4619 overlap by 4 nucleotides
BT4615: BT4615 - chaperone protein dnaK (NCBI ptt file), at 6,041,039 to 6,042,955
BT4615
BT4616: BT4616 - conserved hypothetical protein (NCBI ptt file), at 6,043,159 to 6,043,704
BT4616
BT4617: BT4617 - integrase (NCBI ptt file), at 6,043,701 to 6,044,963
BT4617
BT4618: BT4618 - conserved hypothetical protein (NCBI ptt file), at 6,045,278 to 6,045,631
BT4618
BT4619: BT4619 - hypothetical protein (NCBI ptt file), at 6,045,628 to 6,046,227
BT4619
Position (kb)
6043
6044
6045 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 6042.827 kb on + strand at 6042.904 kb on + strand at 6042.974 kb on + strand at 6043.029 kb on - strand at 6043.070 kb on - strand at 6043.104 kb on - strand at 6043.180 kb on - strand at 6043.230 kb on - strand, within BT4616 at 6043.241 kb on - strand, within BT4616 at 6043.352 kb on + strand at 6043.475 kb on - strand, within BT4616 at 6043.477 kb on - strand, within BT4616 at 6043.556 kb on - strand, within BT4616 at 6043.617 kb on - strand, within BT4616 at 6043.655 kb on - strand at 6043.655 kb on - strand at 6043.691 kb on - strand at 6043.692 kb on + strand at 6043.780 kb on + strand at 6043.811 kb on + strand at 6043.850 kb on + strand, within BT4617 at 6043.914 kb on + strand, within BT4617 at 6043.916 kb on + strand, within BT4617 at 6043.944 kb on - strand, within BT4617 at 6043.983 kb on + strand, within BT4617 at 6044.025 kb on - strand, within BT4617 at 6044.036 kb on - strand, within BT4617 at 6044.038 kb on - strand, within BT4617 at 6044.116 kb on + strand, within BT4617 at 6044.136 kb on - strand, within BT4617 at 6044.136 kb on - strand, within BT4617 at 6044.152 kb on + strand, within BT4617 at 6044.153 kb on - strand, within BT4617 at 6044.246 kb on + strand, within BT4617 at 6044.249 kb on - strand, within BT4617 at 6044.295 kb on - strand, within BT4617 at 6044.298 kb on - strand, within BT4617 at 6044.371 kb on - strand, within BT4617 at 6044.371 kb on - strand, within BT4617 at 6044.384 kb on + strand, within BT4617 at 6044.417 kb on - strand, within BT4617 at 6044.468 kb on + strand, within BT4617 at 6044.534 kb on + strand, within BT4617 at 6044.603 kb on - strand, within BT4617 at 6044.753 kb on - strand, within BT4617 at 6044.802 kb on - strand, within BT4617 at 6044.802 kb on - strand, within BT4617 at 6044.805 kb on + strand, within BT4617 at 6044.805 kb on + strand, within BT4617 at 6044.806 kb on - strand, within BT4617 at 6044.839 kb on + strand at 6044.840 kb on - strand at 6044.841 kb on + strand at 6044.842 kb on - strand at 6044.867 kb on + strand at 6044.868 kb on - strand at 6044.868 kb on - strand at 6044.868 kb on - strand at 6044.871 kb on - strand at 6044.894 kb on + strand at 6044.895 kb on - strand at 6044.975 kb on + strand at 6044.978 kb on - strand at 6045.003 kb on - strand at 6045.020 kb on + strand at 6045.021 kb on - strand at 6045.021 kb on - strand at 6045.223 kb on - strand at 6045.229 kb on + strand at 6045.229 kb on + strand at 6045.231 kb on + strand at 6045.232 kb on - strand at 6045.232 kb on - strand at 6045.267 kb on - strand at 6045.285 kb on - strand at 6045.286 kb on + strand at 6045.292 kb on - strand at 6045.349 kb on - strand, within BT4618 at 6045.415 kb on - strand, within BT4618 at 6045.701 kb on + strand, within BT4619 at 6045.701 kb on + strand, within BT4619 at 6045.702 kb on - strand, within BT4619 at 6045.702 kb on - strand, within BT4619 at 6045.719 kb on - strand, within BT4619 at 6045.725 kb on - strand, within BT4619 at 6045.725 kb on - strand, within BT4619 at 6045.725 kb on - strand, within BT4619 at 6045.820 kb on + strand, within BT4619 at 6045.871 kb on - strand, within BT4619 at 6045.917 kb on + strand, within BT4619 at 6045.938 kb on + strand, within BT4619 at 6045.942 kb on + strand, within BT4619 at 6045.952 kb on + strand, within BT4619
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.025 mM remove 6,042,827 + +1.6 6,042,904 + -0.1 6,042,974 + -2.9 6,043,029 - -0.1 6,043,070 - +0.3 6,043,104 - +0.1 6,043,180 - -1.1 6,043,230 - BT4616 0.13 -2.0 6,043,241 - BT4616 0.15 +2.7 6,043,352 + +5.5 6,043,475 - BT4616 0.58 +0.6 6,043,477 - BT4616 0.58 -2.2 6,043,556 - BT4616 0.73 +2.7 6,043,617 - BT4616 0.84 -2.4 6,043,655 - -0.1 6,043,655 - -3.1 6,043,691 - -1.8 6,043,692 + +0.3 6,043,780 + +0.6 6,043,811 + +1.4 6,043,850 + BT4617 0.12 +1.9 6,043,914 + BT4617 0.17 +1.5 6,043,916 + BT4617 0.17 +2.2 6,043,944 - BT4617 0.19 +0.9 6,043,983 + BT4617 0.22 -3.8 6,044,025 - BT4617 0.26 -2.2 6,044,036 - BT4617 0.27 +1.2 6,044,038 - BT4617 0.27 -0.1 6,044,116 + BT4617 0.33 -0.5 6,044,136 - BT4617 0.34 -2.0 6,044,136 - BT4617 0.34 -1.1 6,044,152 + BT4617 0.36 +0.9 6,044,153 - BT4617 0.36 -2.2 6,044,246 + BT4617 0.43 +0.1 6,044,249 - BT4617 0.43 -0.7 6,044,295 - BT4617 0.47 +0.9 6,044,298 - BT4617 0.47 -3.7 6,044,371 - BT4617 0.53 -1.6 6,044,371 - BT4617 0.53 -2.1 6,044,384 + BT4617 0.54 -1.1 6,044,417 - BT4617 0.57 -1.7 6,044,468 + BT4617 0.61 -1.3 6,044,534 + BT4617 0.66 -4.4 6,044,603 - BT4617 0.71 -1.7 6,044,753 - BT4617 0.83 -3.4 6,044,802 - BT4617 0.87 -3.1 6,044,802 - BT4617 0.87 -0.1 6,044,805 + BT4617 0.87 -1.1 6,044,805 + BT4617 0.87 -2.0 6,044,806 - BT4617 0.87 +1.6 6,044,839 + +1.9 6,044,840 - +2.5 6,044,841 + -4.3 6,044,842 - -2.9 6,044,867 + -0.7 6,044,868 - +0.5 6,044,868 - -1.2 6,044,868 - -1.1 6,044,871 - -2.2 6,044,894 + +2.0 6,044,895 - -1.1 6,044,975 + -1.4 6,044,978 - -1.4 6,045,003 - -0.1 6,045,020 + -2.9 6,045,021 - +0.5 6,045,021 - -0.4 6,045,223 - -2.4 6,045,229 + -2.9 6,045,229 + -2.1 6,045,231 + -4.3 6,045,232 - -0.1 6,045,232 - -2.1 6,045,267 - -2.4 6,045,285 - -2.3 6,045,286 + -2.4 6,045,292 - -3.4 6,045,349 - BT4618 0.20 -0.6 6,045,415 - BT4618 0.39 -1.1 6,045,701 + BT4619 0.12 -1.7 6,045,701 + BT4619 0.12 +0.7 6,045,702 - BT4619 0.12 -1.0 6,045,702 - BT4619 0.12 -0.0 6,045,719 - BT4619 0.15 -0.3 6,045,725 - BT4619 0.16 -3.4 6,045,725 - BT4619 0.16 +1.9 6,045,725 - BT4619 0.16 -1.6 6,045,820 + BT4619 0.32 +1.7 6,045,871 - BT4619 0.41 -1.1 6,045,917 + BT4619 0.48 +1.9 6,045,938 + BT4619 0.52 +1.5 6,045,942 + BT4619 0.52 +2.2 6,045,952 + BT4619 0.54 +1.2
Or see this region's nucleotide sequence