Experiment: BHIS with Thioridazine 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4251 and BT4252 are separated by 39 nucleotides BT4252 and tRNA-Gly are separated by 61 nucleotides tRNA-Gly and tRNA-Tyr are separated by 5 nucleotides tRNA-Tyr and ribH are separated by 50 nucleotides ribH and BT4254 are separated by 167 nucleotides
BT4251: BT4251 - hypothetical protein (NCBI ptt file), at 5,601,336 to 5,603,183
BT4251
BT4252: BT4252 - putative oxidoreductase (NCBI ptt file), at 5,603,223 to 5,604,482
BT4252
BT_t66: tRNA-Gly - tRNA-Gly (RefSeq), at 5,604,544 to 5,604,616
tRNA-Gly
BT_t67: tRNA-Tyr - tRNA-Tyr (RefSeq), at 5,604,622 to 5,604,707
tRNA-Tyr
BT_4253: ribH - riboflavin synthase subunit beta (RefSeq), at 5,604,758 to 5,605,252
ribH
BT4254: BT4254 - conserved hypothetical protein (NCBI ptt file), at 5,605,420 to 5,606,103
BT4254
Position (kb)
5603
5604
5605 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5602.302 kb on - strand, within BT4251 at 5602.310 kb on + strand, within BT4251 at 5602.314 kb on + strand, within BT4251 at 5602.314 kb on + strand, within BT4251 at 5602.315 kb on - strand, within BT4251 at 5602.315 kb on - strand, within BT4251 at 5602.332 kb on - strand, within BT4251 at 5602.341 kb on - strand, within BT4251 at 5602.342 kb on + strand, within BT4251 at 5602.343 kb on - strand, within BT4251 at 5602.343 kb on - strand, within BT4251 at 5602.383 kb on + strand, within BT4251 at 5602.401 kb on - strand, within BT4251 at 5602.407 kb on - strand, within BT4251 at 5602.453 kb on - strand, within BT4251 at 5602.487 kb on + strand, within BT4251 at 5602.577 kb on + strand, within BT4251 at 5602.680 kb on - strand, within BT4251 at 5602.681 kb on + strand, within BT4251 at 5602.681 kb on + strand, within BT4251 at 5602.789 kb on - strand, within BT4251 at 5602.793 kb on + strand, within BT4251 at 5602.841 kb on + strand, within BT4251 at 5602.844 kb on + strand, within BT4251 at 5602.924 kb on + strand, within BT4251 at 5602.925 kb on - strand, within BT4251 at 5602.925 kb on - strand, within BT4251 at 5603.136 kb on + strand at 5603.137 kb on - strand at 5603.149 kb on - strand at 5603.170 kb on - strand at 5603.199 kb on + strand at 5603.200 kb on + strand at 5603.201 kb on - strand at 5603.213 kb on + strand at 5603.280 kb on - strand at 5603.318 kb on + strand at 5603.400 kb on + strand, within BT4252 at 5603.404 kb on + strand, within BT4252 at 5603.405 kb on - strand, within BT4252 at 5603.471 kb on + strand, within BT4252 at 5603.481 kb on + strand, within BT4252 at 5603.481 kb on + strand, within BT4252 at 5603.485 kb on - strand, within BT4252 at 5603.516 kb on + strand, within BT4252 at 5603.516 kb on + strand, within BT4252 at 5603.516 kb on + strand, within BT4252 at 5603.576 kb on + strand, within BT4252 at 5603.624 kb on + strand, within BT4252 at 5603.690 kb on + strand, within BT4252 at 5603.690 kb on + strand, within BT4252 at 5603.691 kb on - strand, within BT4252 at 5603.783 kb on - strand, within BT4252 at 5603.861 kb on + strand, within BT4252 at 5603.862 kb on - strand, within BT4252 at 5603.926 kb on + strand, within BT4252 at 5603.926 kb on + strand, within BT4252 at 5603.949 kb on - strand, within BT4252 at 5603.950 kb on + strand, within BT4252 at 5603.950 kb on + strand, within BT4252 at 5603.970 kb on + strand, within BT4252 at 5604.009 kb on - strand, within BT4252 at 5604.029 kb on - strand, within BT4252 at 5604.050 kb on + strand, within BT4252 at 5604.050 kb on + strand, within BT4252 at 5604.129 kb on - strand, within BT4252 at 5604.132 kb on + strand, within BT4252 at 5604.132 kb on + strand, within BT4252 at 5604.132 kb on + strand, within BT4252 at 5604.133 kb on - strand, within BT4252 at 5604.133 kb on - strand, within BT4252 at 5604.133 kb on - strand, within BT4252 at 5604.157 kb on + strand, within BT4252 at 5604.157 kb on + strand, within BT4252 at 5604.192 kb on - strand, within BT4252 at 5604.256 kb on + strand, within BT4252 at 5604.257 kb on - strand, within BT4252 at 5604.257 kb on - strand, within BT4252 at 5604.384 kb on + strand at 5604.385 kb on - strand at 5604.385 kb on - strand at 5604.406 kb on - strand at 5604.453 kb on + strand at 5604.496 kb on + strand at 5604.496 kb on + strand at 5604.496 kb on + strand at 5605.342 kb on - strand at 5605.342 kb on - strand at 5605.358 kb on - strand at 5605.400 kb on - strand at 5605.405 kb on - strand at 5605.407 kb on - strand at 5605.422 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.025 mM remove 5,602,302 - BT4251 0.52 +3.2 5,602,310 + BT4251 0.53 +3.7 5,602,314 + BT4251 0.53 +0.0 5,602,314 + BT4251 0.53 +1.0 5,602,315 - BT4251 0.53 -1.0 5,602,315 - BT4251 0.53 -1.0 5,602,332 - BT4251 0.54 -1.1 5,602,341 - BT4251 0.54 -1.2 5,602,342 + BT4251 0.54 -1.5 5,602,343 - BT4251 0.54 +1.9 5,602,343 - BT4251 0.54 -1.3 5,602,383 + BT4251 0.57 -3.0 5,602,401 - BT4251 0.58 -3.8 5,602,407 - BT4251 0.58 +1.6 5,602,453 - BT4251 0.60 +2.8 5,602,487 + BT4251 0.62 -2.8 5,602,577 + BT4251 0.67 +1.0 5,602,680 - BT4251 0.73 -2.3 5,602,681 + BT4251 0.73 +3.2 5,602,681 + BT4251 0.73 +1.0 5,602,789 - BT4251 0.79 -2.3 5,602,793 + BT4251 0.79 -1.0 5,602,841 + BT4251 0.81 +1.0 5,602,844 + BT4251 0.82 +2.4 5,602,924 + BT4251 0.86 +1.6 5,602,925 - BT4251 0.86 +0.2 5,602,925 - BT4251 0.86 -1.4 5,603,136 + -1.0 5,603,137 - -1.0 5,603,149 - +0.4 5,603,170 - -2.3 5,603,199 + +0.0 5,603,200 + -1.3 5,603,201 - -0.5 5,603,213 + -1.0 5,603,280 - -1.6 5,603,318 + -1.2 5,603,400 + BT4252 0.14 -1.6 5,603,404 + BT4252 0.14 -1.5 5,603,405 - BT4252 0.14 -1.4 5,603,471 + BT4252 0.20 -2.8 5,603,481 + BT4252 0.20 -1.3 5,603,481 + BT4252 0.20 -2.3 5,603,485 - BT4252 0.21 -1.3 5,603,516 + BT4252 0.23 +0.6 5,603,516 + BT4252 0.23 -1.0 5,603,516 + BT4252 0.23 -2.6 5,603,576 + BT4252 0.28 +1.8 5,603,624 + BT4252 0.32 -1.3 5,603,690 + BT4252 0.37 -2.6 5,603,690 + BT4252 0.37 +0.4 5,603,691 - BT4252 0.37 -1.0 5,603,783 - BT4252 0.44 -0.6 5,603,861 + BT4252 0.51 +0.0 5,603,862 - BT4252 0.51 -1.1 5,603,926 + BT4252 0.56 +0.4 5,603,926 + BT4252 0.56 -1.0 5,603,949 - BT4252 0.58 -2.6 5,603,950 + BT4252 0.58 -0.9 5,603,950 + BT4252 0.58 -2.0 5,603,970 + BT4252 0.59 +0.5 5,604,009 - BT4252 0.62 +1.0 5,604,029 - BT4252 0.64 +0.0 5,604,050 + BT4252 0.66 -3.0 5,604,050 + BT4252 0.66 -2.2 5,604,129 - BT4252 0.72 -1.4 5,604,132 + BT4252 0.72 -2.4 5,604,132 + BT4252 0.72 -3.6 5,604,132 + BT4252 0.72 +1.0 5,604,133 - BT4252 0.72 -1.3 5,604,133 - BT4252 0.72 -1.3 5,604,133 - BT4252 0.72 +1.0 5,604,157 + BT4252 0.74 -1.9 5,604,157 + BT4252 0.74 -2.0 5,604,192 - BT4252 0.77 -1.6 5,604,256 + BT4252 0.82 +0.0 5,604,257 - BT4252 0.82 -0.7 5,604,257 - BT4252 0.82 -0.7 5,604,384 + +0.7 5,604,385 - +0.0 5,604,385 - -3.4 5,604,406 - -2.6 5,604,453 + -2.8 5,604,496 + -2.6 5,604,496 + +0.0 5,604,496 + +1.3 5,605,342 - -0.7 5,605,342 - -1.7 5,605,358 - -2.8 5,605,400 - -1.8 5,605,405 - -1.8 5,605,407 - -0.7 5,605,422 - +0.0
Or see this region's nucleotide sequence