Experiment: BHIS with Thioridazine 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3952 and BT3953 are separated by 60 nucleotides BT3953 and BT3954 are separated by 37 nucleotides
BT3952: BT3952 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 5,132,450 to 5,135,593
BT3952
BT3953: BT3953 - conserved hypothetical protein, putative outer membrane protein (NCBI ptt file), at 5,135,654 to 5,137,549
BT3953
BT3954: BT3954 - hypothetical protein (NCBI ptt file), at 5,137,587 to 5,138,942
BT3954
Position (kb)
5135
5136
5137
5138 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5134.708 kb on + strand, within BT3952 at 5134.746 kb on - strand, within BT3952 at 5134.777 kb on + strand, within BT3952 at 5134.927 kb on - strand, within BT3952 at 5135.010 kb on + strand, within BT3952 at 5135.017 kb on - strand, within BT3952 at 5135.026 kb on - strand, within BT3952 at 5135.067 kb on - strand, within BT3952 at 5135.102 kb on - strand, within BT3952 at 5135.111 kb on + strand, within BT3952 at 5135.149 kb on + strand, within BT3952 at 5135.315 kb on + strand at 5135.315 kb on + strand at 5135.407 kb on - strand at 5135.412 kb on - strand at 5135.413 kb on + strand at 5135.414 kb on - strand at 5135.416 kb on + strand at 5135.417 kb on - strand at 5135.417 kb on - strand at 5135.470 kb on + strand at 5135.598 kb on - strand at 5135.607 kb on + strand at 5135.615 kb on - strand at 5135.623 kb on + strand at 5135.629 kb on - strand at 5135.757 kb on - strand at 5135.770 kb on - strand at 5135.770 kb on - strand at 5135.771 kb on + strand at 5135.772 kb on - strand at 5135.778 kb on - strand at 5135.873 kb on + strand, within BT3953 at 5135.943 kb on - strand, within BT3953 at 5135.952 kb on + strand, within BT3953 at 5135.965 kb on + strand, within BT3953 at 5135.965 kb on + strand, within BT3953 at 5135.986 kb on + strand, within BT3953 at 5135.987 kb on - strand, within BT3953 at 5136.059 kb on + strand, within BT3953 at 5136.256 kb on + strand, within BT3953 at 5136.256 kb on + strand, within BT3953 at 5136.257 kb on - strand, within BT3953 at 5136.274 kb on + strand, within BT3953 at 5136.274 kb on + strand, within BT3953 at 5136.275 kb on - strand, within BT3953 at 5136.303 kb on - strand, within BT3953 at 5136.332 kb on - strand, within BT3953 at 5136.461 kb on - strand, within BT3953 at 5136.500 kb on + strand, within BT3953 at 5136.588 kb on - strand, within BT3953 at 5136.593 kb on + strand, within BT3953 at 5136.595 kb on + strand, within BT3953 at 5136.595 kb on + strand, within BT3953 at 5136.595 kb on + strand, within BT3953 at 5136.595 kb on + strand, within BT3953 at 5136.596 kb on - strand, within BT3953 at 5136.600 kb on - strand, within BT3953 at 5136.606 kb on + strand, within BT3953 at 5136.660 kb on - strand, within BT3953 at 5136.663 kb on - strand, within BT3953 at 5136.664 kb on + strand, within BT3953 at 5136.665 kb on - strand, within BT3953 at 5136.665 kb on - strand, within BT3953 at 5136.785 kb on + strand, within BT3953 at 5136.785 kb on + strand, within BT3953 at 5136.785 kb on + strand, within BT3953 at 5136.881 kb on - strand, within BT3953 at 5136.881 kb on - strand, within BT3953 at 5136.887 kb on + strand, within BT3953 at 5137.100 kb on + strand, within BT3953 at 5137.149 kb on - strand, within BT3953 at 5137.189 kb on + strand, within BT3953 at 5137.190 kb on - strand, within BT3953 at 5137.190 kb on - strand, within BT3953 at 5137.289 kb on - strand at 5137.398 kb on - strand at 5137.409 kb on - strand at 5137.414 kb on + strand at 5137.462 kb on - strand at 5137.462 kb on - strand at 5137.499 kb on + strand at 5137.500 kb on - strand at 5137.540 kb on - strand at 5137.567 kb on - strand at 5137.607 kb on - strand at 5137.684 kb on - strand at 5137.684 kb on - strand at 5137.719 kb on - strand at 5137.761 kb on + strand, within BT3954 at 5137.806 kb on - strand, within BT3954 at 5137.806 kb on - strand, within BT3954 at 5137.833 kb on + strand, within BT3954 at 5137.833 kb on + strand, within BT3954 at 5137.834 kb on - strand, within BT3954 at 5137.849 kb on + strand, within BT3954 at 5137.980 kb on + strand, within BT3954 at 5137.986 kb on - strand, within BT3954 at 5138.037 kb on - strand, within BT3954 at 5138.103 kb on - strand, within BT3954 at 5138.107 kb on - strand, within BT3954 at 5138.131 kb on - strand, within BT3954 at 5138.131 kb on - strand, within BT3954 at 5138.223 kb on + strand, within BT3954 at 5138.223 kb on + strand, within BT3954 at 5138.224 kb on - strand, within BT3954 at 5138.284 kb on - strand, within BT3954 at 5138.286 kb on - strand, within BT3954 at 5138.286 kb on - strand, within BT3954 at 5138.300 kb on + strand, within BT3954 at 5138.318 kb on + strand, within BT3954 at 5138.338 kb on - strand, within BT3954 at 5138.385 kb on + strand, within BT3954 at 5138.385 kb on + strand, within BT3954 at 5138.544 kb on + strand, within BT3954
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.025 mM remove 5,134,708 + BT3952 0.72 -2.8 5,134,746 - BT3952 0.73 +0.0 5,134,777 + BT3952 0.74 +2.3 5,134,927 - BT3952 0.79 +2.3 5,135,010 + BT3952 0.81 -0.7 5,135,017 - BT3952 0.82 -1.4 5,135,026 - BT3952 0.82 -0.8 5,135,067 - BT3952 0.83 -1.0 5,135,102 - BT3952 0.84 -1.6 5,135,111 + BT3952 0.85 +1.0 5,135,149 + BT3952 0.86 -0.5 5,135,315 + -1.0 5,135,315 + -1.8 5,135,407 - +2.0 5,135,412 - -3.0 5,135,413 + -1.0 5,135,414 - -2.6 5,135,416 + -1.0 5,135,417 - -1.6 5,135,417 - +1.9 5,135,470 + +2.3 5,135,598 - -1.9 5,135,607 + -1.6 5,135,615 - -2.0 5,135,623 + +0.4 5,135,629 - -1.6 5,135,757 - -3.1 5,135,770 - -0.7 5,135,770 - +2.2 5,135,771 + +1.3 5,135,772 - -0.8 5,135,778 - +1.2 5,135,873 + BT3953 0.12 +2.0 5,135,943 - BT3953 0.15 -1.3 5,135,952 + BT3953 0.16 +0.3 5,135,965 + BT3953 0.16 -2.0 5,135,965 + BT3953 0.16 -1.6 5,135,986 + BT3953 0.18 -1.0 5,135,987 - BT3953 0.18 +0.0 5,136,059 + BT3953 0.21 -1.7 5,136,256 + BT3953 0.32 -2.9 5,136,256 + BT3953 0.32 -1.6 5,136,257 - BT3953 0.32 -2.6 5,136,274 + BT3953 0.33 -1.8 5,136,274 + BT3953 0.33 -1.1 5,136,275 - BT3953 0.33 +1.9 5,136,303 - BT3953 0.34 -2.6 5,136,332 - BT3953 0.36 -1.7 5,136,461 - BT3953 0.43 +0.9 5,136,500 + BT3953 0.45 +0.0 5,136,588 - BT3953 0.49 -1.0 5,136,593 + BT3953 0.50 -1.6 5,136,595 + BT3953 0.50 -1.7 5,136,595 + BT3953 0.50 -1.0 5,136,595 + BT3953 0.50 -3.0 5,136,595 + BT3953 0.50 -3.5 5,136,596 - BT3953 0.50 +2.0 5,136,600 - BT3953 0.50 -1.4 5,136,606 + BT3953 0.50 +2.6 5,136,660 - BT3953 0.53 +2.8 5,136,663 - BT3953 0.53 -1.0 5,136,664 + BT3953 0.53 +2.6 5,136,665 - BT3953 0.53 -1.8 5,136,665 - BT3953 0.53 -1.0 5,136,785 + BT3953 0.60 -1.6 5,136,785 + BT3953 0.60 +1.0 5,136,785 + BT3953 0.60 -3.0 5,136,881 - BT3953 0.65 +0.6 5,136,881 - BT3953 0.65 -1.2 5,136,887 + BT3953 0.65 +1.0 5,137,100 + BT3953 0.76 -2.2 5,137,149 - BT3953 0.79 +0.0 5,137,189 + BT3953 0.81 +2.8 5,137,190 - BT3953 0.81 -1.4 5,137,190 - BT3953 0.81 -3.7 5,137,289 - -1.0 5,137,398 - -1.4 5,137,409 - -3.3 5,137,414 + +1.6 5,137,462 - -0.7 5,137,462 - -2.6 5,137,499 + -0.6 5,137,500 - +3.7 5,137,540 - -1.5 5,137,567 - -1.3 5,137,607 - -2.6 5,137,684 - +2.2 5,137,684 - +0.0 5,137,719 - +1.8 5,137,761 + BT3954 0.13 +2.0 5,137,806 - BT3954 0.16 -0.1 5,137,806 - BT3954 0.16 -2.0 5,137,833 + BT3954 0.18 +0.6 5,137,833 + BT3954 0.18 -3.2 5,137,834 - BT3954 0.18 -0.1 5,137,849 + BT3954 0.19 +0.0 5,137,980 + BT3954 0.29 -1.0 5,137,986 - BT3954 0.29 -1.3 5,138,037 - BT3954 0.33 -2.6 5,138,103 - BT3954 0.38 -0.8 5,138,107 - BT3954 0.38 -1.6 5,138,131 - BT3954 0.40 +3.7 5,138,131 - BT3954 0.40 -2.3 5,138,223 + BT3954 0.47 +1.6 5,138,223 + BT3954 0.47 -1.2 5,138,224 - BT3954 0.47 -1.6 5,138,284 - BT3954 0.51 -1.6 5,138,286 - BT3954 0.52 -1.3 5,138,286 - BT3954 0.52 -4.3 5,138,300 + BT3954 0.53 -2.3 5,138,318 + BT3954 0.54 -0.7 5,138,338 - BT3954 0.55 -1.0 5,138,385 + BT3954 0.59 +2.6 5,138,385 + BT3954 0.59 +1.0 5,138,544 + BT3954 0.71 +0.0
Or see this region's nucleotide sequence