Experiment: BHIS with Thioridazine 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3861 and BT3862 are separated by 74 nucleotides BT3862 and BT3863 are separated by 80 nucleotides
BT3861: BT3861 - conserved hypothetical protein (NCBI ptt file), at 5,029,186 to 5,030,328
BT3861
BT3862: BT3862 - endo-alpha-mannosidase (NCBI ptt file), at 5,030,403 to 5,031,551
BT3862
BT3863: BT3863 - conserved hypothetical protein (NCBI ptt file), at 5,031,632 to 5,034,007
BT3863
Position (kb)
5030
5031
5032 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5029.557 kb on + strand, within BT3861 at 5029.557 kb on + strand, within BT3861 at 5029.615 kb on + strand, within BT3861 at 5029.717 kb on + strand, within BT3861 at 5029.718 kb on - strand, within BT3861 at 5029.726 kb on - strand, within BT3861 at 5029.726 kb on - strand, within BT3861 at 5029.733 kb on + strand, within BT3861 at 5029.734 kb on - strand, within BT3861 at 5029.734 kb on - strand, within BT3861 at 5029.821 kb on + strand, within BT3861 at 5029.829 kb on - strand, within BT3861 at 5029.829 kb on - strand, within BT3861 at 5029.829 kb on - strand, within BT3861 at 5029.835 kb on + strand, within BT3861 at 5029.836 kb on - strand, within BT3861 at 5029.881 kb on - strand, within BT3861 at 5029.969 kb on + strand, within BT3861 at 5030.023 kb on + strand, within BT3861 at 5030.024 kb on - strand, within BT3861 at 5030.026 kb on - strand, within BT3861 at 5030.026 kb on - strand, within BT3861 at 5030.026 kb on - strand, within BT3861 at 5030.026 kb on - strand, within BT3861 at 5030.038 kb on - strand, within BT3861 at 5030.050 kb on - strand, within BT3861 at 5030.115 kb on + strand, within BT3861 at 5030.115 kb on + strand, within BT3861 at 5030.116 kb on - strand, within BT3861 at 5030.116 kb on - strand, within BT3861 at 5030.235 kb on + strand at 5030.236 kb on - strand at 5030.257 kb on - strand at 5030.315 kb on + strand at 5030.326 kb on + strand at 5030.383 kb on - strand at 5030.391 kb on - strand at 5030.399 kb on + strand at 5030.437 kb on + strand at 5030.525 kb on + strand, within BT3862 at 5030.525 kb on + strand, within BT3862 at 5030.526 kb on - strand, within BT3862 at 5030.543 kb on + strand, within BT3862 at 5030.648 kb on + strand, within BT3862 at 5030.726 kb on + strand, within BT3862 at 5030.741 kb on - strand, within BT3862 at 5030.741 kb on - strand, within BT3862 at 5030.741 kb on - strand, within BT3862 at 5030.741 kb on - strand, within BT3862 at 5030.759 kb on - strand, within BT3862 at 5030.858 kb on + strand, within BT3862 at 5030.859 kb on - strand, within BT3862 at 5030.911 kb on - strand, within BT3862 at 5030.911 kb on - strand, within BT3862 at 5030.912 kb on + strand, within BT3862 at 5030.912 kb on + strand, within BT3862 at 5030.912 kb on + strand, within BT3862 at 5030.912 kb on + strand, within BT3862 at 5030.912 kb on + strand, within BT3862 at 5030.912 kb on + strand, within BT3862 at 5030.913 kb on - strand, within BT3862 at 5030.922 kb on - strand, within BT3862 at 5030.926 kb on - strand, within BT3862 at 5030.979 kb on + strand, within BT3862 at 5030.979 kb on + strand, within BT3862 at 5030.979 kb on + strand, within BT3862 at 5031.040 kb on + strand, within BT3862 at 5031.040 kb on + strand, within BT3862 at 5031.040 kb on + strand, within BT3862 at 5031.041 kb on - strand, within BT3862 at 5031.050 kb on + strand, within BT3862 at 5031.051 kb on - strand, within BT3862 at 5031.060 kb on + strand, within BT3862 at 5031.176 kb on + strand, within BT3862 at 5031.222 kb on - strand, within BT3862 at 5031.224 kb on + strand, within BT3862 at 5031.257 kb on + strand, within BT3862 at 5031.329 kb on + strand, within BT3862 at 5031.329 kb on + strand, within BT3862 at 5031.334 kb on - strand, within BT3862 at 5031.335 kb on + strand, within BT3862 at 5031.336 kb on - strand, within BT3862 at 5031.363 kb on + strand, within BT3862 at 5031.458 kb on + strand at 5031.459 kb on - strand at 5031.505 kb on + strand at 5031.549 kb on + strand at 5031.550 kb on - strand at 5031.612 kb on + strand at 5031.613 kb on - strand at 5031.613 kb on - strand at 5031.622 kb on - strand at 5031.624 kb on - strand at 5031.645 kb on - strand at 5031.798 kb on + strand at 5031.799 kb on - strand at 5031.816 kb on + strand at 5031.839 kb on - strand at 5031.845 kb on - strand at 5031.847 kb on - strand at 5031.959 kb on - strand, within BT3863 at 5032.077 kb on - strand, within BT3863 at 5032.152 kb on + strand, within BT3863 at 5032.201 kb on - strand, within BT3863 at 5032.201 kb on - strand, within BT3863 at 5032.281 kb on + strand, within BT3863 at 5032.369 kb on + strand, within BT3863 at 5032.369 kb on + strand, within BT3863 at 5032.377 kb on + strand, within BT3863 at 5032.400 kb on - strand, within BT3863 at 5032.400 kb on - strand, within BT3863 at 5032.436 kb on - strand, within BT3863 at 5032.514 kb on + strand, within BT3863 at 5032.515 kb on - strand, within BT3863 at 5032.516 kb on + strand, within BT3863
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.025 mM remove 5,029,557 + BT3861 0.32 -1.0 5,029,557 + BT3861 0.32 -2.8 5,029,615 + BT3861 0.38 +0.8 5,029,717 + BT3861 0.46 +1.8 5,029,718 - BT3861 0.47 -1.3 5,029,726 - BT3861 0.47 -1.5 5,029,726 - BT3861 0.47 -1.4 5,029,733 + BT3861 0.48 -0.4 5,029,734 - BT3861 0.48 -1.0 5,029,734 - BT3861 0.48 +1.0 5,029,821 + BT3861 0.56 -0.1 5,029,829 - BT3861 0.56 +0.0 5,029,829 - BT3861 0.56 +0.0 5,029,829 - BT3861 0.56 -4.3 5,029,835 + BT3861 0.57 -0.3 5,029,836 - BT3861 0.57 -1.6 5,029,881 - BT3861 0.61 -1.6 5,029,969 + BT3861 0.69 -0.3 5,030,023 + BT3861 0.73 +1.0 5,030,024 - BT3861 0.73 -1.6 5,030,026 - BT3861 0.73 +0.0 5,030,026 - BT3861 0.73 -1.4 5,030,026 - BT3861 0.73 +0.6 5,030,026 - BT3861 0.73 +1.2 5,030,038 - BT3861 0.75 +2.9 5,030,050 - BT3861 0.76 -2.0 5,030,115 + BT3861 0.81 +1.2 5,030,115 + BT3861 0.81 +1.0 5,030,116 - BT3861 0.81 -2.0 5,030,116 - BT3861 0.81 -0.8 5,030,235 + +2.0 5,030,236 - +1.9 5,030,257 - -3.3 5,030,315 + -2.3 5,030,326 + -1.6 5,030,383 - +0.3 5,030,391 - -0.9 5,030,399 + -0.8 5,030,437 + +1.6 5,030,525 + BT3862 0.11 +0.6 5,030,525 + BT3862 0.11 -0.4 5,030,526 - BT3862 0.11 +0.5 5,030,543 + BT3862 0.12 +0.2 5,030,648 + BT3862 0.21 -1.6 5,030,726 + BT3862 0.28 +0.0 5,030,741 - BT3862 0.29 +1.2 5,030,741 - BT3862 0.29 +1.6 5,030,741 - BT3862 0.29 -0.5 5,030,741 - BT3862 0.29 -4.7 5,030,759 - BT3862 0.31 +0.8 5,030,858 + BT3862 0.40 -1.4 5,030,859 - BT3862 0.40 -2.8 5,030,911 - BT3862 0.44 -1.2 5,030,911 - BT3862 0.44 -1.6 5,030,912 + BT3862 0.44 +2.8 5,030,912 + BT3862 0.44 -2.3 5,030,912 + BT3862 0.44 +1.4 5,030,912 + BT3862 0.44 +1.3 5,030,912 + BT3862 0.44 +1.8 5,030,912 + BT3862 0.44 +2.1 5,030,913 - BT3862 0.44 +2.8 5,030,922 - BT3862 0.45 -1.6 5,030,926 - BT3862 0.46 +1.3 5,030,979 + BT3862 0.50 +1.3 5,030,979 + BT3862 0.50 -0.5 5,030,979 + BT3862 0.50 +1.0 5,031,040 + BT3862 0.55 -2.3 5,031,040 + BT3862 0.55 +2.6 5,031,040 + BT3862 0.55 -3.3 5,031,041 - BT3862 0.56 -0.5 5,031,050 + BT3862 0.56 -2.0 5,031,051 - BT3862 0.56 +0.0 5,031,060 + BT3862 0.57 +1.6 5,031,176 + BT3862 0.67 -2.1 5,031,222 - BT3862 0.71 -1.0 5,031,224 + BT3862 0.71 -1.9 5,031,257 + BT3862 0.74 -1.6 5,031,329 + BT3862 0.81 -2.3 5,031,329 + BT3862 0.81 -1.4 5,031,334 - BT3862 0.81 -0.6 5,031,335 + BT3862 0.81 -2.3 5,031,336 - BT3862 0.81 -1.7 5,031,363 + BT3862 0.84 +1.6 5,031,458 + +0.2 5,031,459 - -1.8 5,031,505 + -3.4 5,031,549 + +1.8 5,031,550 - -1.0 5,031,612 + -1.6 5,031,613 - +1.3 5,031,613 - -3.1 5,031,622 - -0.5 5,031,624 - -4.0 5,031,645 - +0.0 5,031,798 + -2.3 5,031,799 - -1.3 5,031,816 + -3.2 5,031,839 - -0.7 5,031,845 - +2.8 5,031,847 - -2.0 5,031,959 - BT3863 0.14 -1.4 5,032,077 - BT3863 0.19 +0.0 5,032,152 + BT3863 0.22 -1.7 5,032,201 - BT3863 0.24 +0.0 5,032,201 - BT3863 0.24 +0.0 5,032,281 + BT3863 0.27 +0.3 5,032,369 + BT3863 0.31 +3.7 5,032,369 + BT3863 0.31 -2.6 5,032,377 + BT3863 0.31 +0.0 5,032,400 - BT3863 0.32 +1.2 5,032,400 - BT3863 0.32 +1.0 5,032,436 - BT3863 0.34 -1.1 5,032,514 + BT3863 0.37 -2.4 5,032,515 - BT3863 0.37 -3.1 5,032,516 + BT3863 0.37 -2.3
Or see this region's nucleotide sequence