Experiment: BHIS with Thioridazine 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2993 and BT2994 are separated by 178 nucleotides BT2994 and BT2995 are separated by 15 nucleotides
BT2993: BT2993 - hypothetical protein (NCBI ptt file), at 3,774,175 to 3,774,993
BT2993
BT2994: BT2994 - conserved hypothetical protein (NCBI ptt file), at 3,775,172 to 3,775,579
BT2994
BT2995: BT2995 - conserved hypothetical protein with relaxase/mobilization nuclease domain (NCBI ptt file), at 3,775,595 to 3,776,806
BT2995
Position (kb)
3775
3776 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3774.196 kb on + strand at 3774.196 kb on + strand at 3774.197 kb on - strand at 3774.202 kb on - strand at 3774.206 kb on + strand at 3774.229 kb on + strand at 3774.248 kb on - strand at 3774.422 kb on + strand, within BT2993 at 3774.525 kb on + strand, within BT2993 at 3774.542 kb on - strand, within BT2993 at 3774.606 kb on + strand, within BT2993 at 3774.606 kb on + strand, within BT2993 at 3774.606 kb on + strand, within BT2993 at 3774.707 kb on - strand, within BT2993 at 3774.835 kb on - strand, within BT2993 at 3774.919 kb on - strand at 3774.963 kb on + strand at 3774.990 kb on - strand at 3775.005 kb on - strand at 3775.020 kb on - strand at 3775.020 kb on - strand at 3775.027 kb on - strand at 3775.029 kb on + strand at 3775.034 kb on - strand at 3775.053 kb on - strand at 3775.053 kb on - strand at 3775.090 kb on - strand at 3775.090 kb on - strand at 3775.090 kb on - strand at 3775.090 kb on - strand at 3775.090 kb on - strand at 3775.186 kb on + strand at 3775.334 kb on + strand, within BT2994 at 3775.334 kb on + strand, within BT2994 at 3775.335 kb on - strand, within BT2994 at 3775.335 kb on - strand, within BT2994 at 3775.405 kb on + strand, within BT2994 at 3775.406 kb on + strand, within BT2994 at 3775.406 kb on - strand, within BT2994 at 3775.644 kb on - strand at 3775.644 kb on - strand at 3775.655 kb on - strand at 3775.669 kb on + strand at 3775.671 kb on + strand at 3775.671 kb on + strand at 3775.671 kb on + strand at 3775.672 kb on - strand at 3775.750 kb on + strand, within BT2995 at 3775.750 kb on + strand, within BT2995 at 3775.795 kb on - strand, within BT2995 at 3775.893 kb on + strand, within BT2995 at 3775.903 kb on + strand, within BT2995 at 3775.969 kb on - strand, within BT2995 at 3776.026 kb on + strand, within BT2995 at 3776.026 kb on + strand, within BT2995 at 3776.026 kb on + strand, within BT2995 at 3776.027 kb on - strand, within BT2995 at 3776.027 kb on - strand, within BT2995 at 3776.074 kb on - strand, within BT2995 at 3776.086 kb on + strand, within BT2995 at 3776.134 kb on - strand, within BT2995 at 3776.241 kb on + strand, within BT2995 at 3776.247 kb on + strand, within BT2995 at 3776.308 kb on + strand, within BT2995 at 3776.308 kb on + strand, within BT2995 at 3776.308 kb on + strand, within BT2995 at 3776.356 kb on + strand, within BT2995 at 3776.482 kb on - strand, within BT2995 at 3776.492 kb on + strand, within BT2995 at 3776.492 kb on + strand, within BT2995 at 3776.493 kb on - strand, within BT2995
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.025 mM remove 3,774,196 + -1.6 3,774,196 + -1.9 3,774,197 - -2.3 3,774,202 - -1.6 3,774,206 + -0.1 3,774,229 + -2.5 3,774,248 - +0.1 3,774,422 + BT2993 0.30 -1.0 3,774,525 + BT2993 0.43 -1.9 3,774,542 - BT2993 0.45 -2.1 3,774,606 + BT2993 0.53 +1.7 3,774,606 + BT2993 0.53 +1.1 3,774,606 + BT2993 0.53 -1.5 3,774,707 - BT2993 0.65 -2.8 3,774,835 - BT2993 0.81 +0.1 3,774,919 - +1.1 3,774,963 + -1.5 3,774,990 - +3.1 3,775,005 - -1.2 3,775,020 - -1.9 3,775,020 - -2.5 3,775,027 - +1.9 3,775,029 + +0.1 3,775,034 - +0.9 3,775,053 - +1.1 3,775,053 - +1.7 3,775,090 - -2.1 3,775,090 - -2.5 3,775,090 - +3.1 3,775,090 - +2.4 3,775,090 - -1.5 3,775,186 + -0.9 3,775,334 + BT2994 0.40 -2.4 3,775,334 + BT2994 0.40 -2.1 3,775,335 - BT2994 0.40 -1.0 3,775,335 - BT2994 0.40 +2.9 3,775,405 + BT2994 0.57 -1.7 3,775,406 + BT2994 0.57 -3.2 3,775,406 - BT2994 0.57 +1.7 3,775,644 - +0.5 3,775,644 - -0.9 3,775,655 - +0.8 3,775,669 + +3.3 3,775,671 + +1.8 3,775,671 + +0.4 3,775,671 + -2.3 3,775,672 - -0.9 3,775,750 + BT2995 0.13 -1.2 3,775,750 + BT2995 0.13 -2.9 3,775,795 - BT2995 0.17 -1.0 3,775,893 + BT2995 0.25 -1.8 3,775,903 + BT2995 0.25 -1.1 3,775,969 - BT2995 0.31 -1.7 3,776,026 + BT2995 0.36 +3.4 3,776,026 + BT2995 0.36 -0.6 3,776,026 + BT2995 0.36 -3.2 3,776,027 - BT2995 0.36 +2.8 3,776,027 - BT2995 0.36 +2.2 3,776,074 - BT2995 0.40 +0.7 3,776,086 + BT2995 0.41 +0.8 3,776,134 - BT2995 0.44 -1.5 3,776,241 + BT2995 0.53 +0.4 3,776,247 + BT2995 0.54 +2.9 3,776,308 + BT2995 0.59 -1.7 3,776,308 + BT2995 0.59 -1.6 3,776,308 + BT2995 0.59 +2.1 3,776,356 + BT2995 0.63 -0.8 3,776,482 - BT2995 0.73 -0.5 3,776,492 + BT2995 0.74 -0.4 3,776,492 + BT2995 0.74 +1.5 3,776,493 - BT2995 0.74 -1.2
Or see this region's nucleotide sequence