Experiment: BHIS with Thioridazine 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2279 and BT2280 are separated by 1 nucleotides BT2280 and BT2281 are separated by 186 nucleotides BT2281 and BT2282 are separated by 757 nucleotides
BT2279: BT2279 - putative ribose phosphate pyrophosphokinase (NCBI ptt file), at 2,859,673 to 2,860,593
BT2279
BT2280: BT2280 - hypothetical protein (NCBI ptt file), at 2,860,595 to 2,860,969
BT2280
BT2281: BT2281 - hypothetical protein (NCBI ptt file), at 2,861,156 to 2,861,428
BT2281
BT2282: BT2282 - conserved hypothetical protein (NCBI ptt file), at 2,862,186 to 2,862,758
BT2282
Position (kb)
2861
2862 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6
7 at 2860.156 kb on - strand, within BT2279 at 2860.161 kb on + strand, within BT2279 at 2860.466 kb on + strand, within BT2279 at 2860.479 kb on + strand, within BT2279 at 2860.479 kb on + strand, within BT2279 at 2860.668 kb on + strand, within BT2280 at 2860.668 kb on + strand, within BT2280 at 2860.669 kb on - strand, within BT2280 at 2860.810 kb on - strand, within BT2280 at 2860.856 kb on - strand, within BT2280 at 2860.882 kb on - strand, within BT2280 at 2860.912 kb on + strand, within BT2280 at 2860.979 kb on + strand at 2860.980 kb on - strand at 2860.980 kb on - strand at 2860.980 kb on - strand at 2860.982 kb on + strand at 2860.982 kb on + strand at 2861.036 kb on + strand at 2861.059 kb on + strand at 2861.093 kb on + strand at 2861.093 kb on + strand at 2861.094 kb on - strand at 2861.094 kb on - strand at 2861.142 kb on - strand at 2861.142 kb on - strand at 2861.182 kb on + strand at 2861.182 kb on + strand at 2861.186 kb on + strand, within BT2281 at 2861.197 kb on - strand, within BT2281 at 2861.304 kb on - strand, within BT2281 at 2861.342 kb on - strand, within BT2281 at 2861.367 kb on + strand, within BT2281 at 2861.367 kb on + strand, within BT2281 at 2861.428 kb on + strand at 2861.440 kb on + strand at 2861.441 kb on - strand at 2861.444 kb on - strand at 2861.448 kb on + strand at 2861.521 kb on + strand at 2861.536 kb on - strand at 2861.536 kb on - strand at 2861.536 kb on - strand at 2861.536 kb on - strand at 2861.536 kb on - strand at 2861.561 kb on + strand at 2861.617 kb on - strand at 2861.617 kb on - strand at 2861.668 kb on - strand at 2861.668 kb on - strand at 2861.671 kb on + strand at 2861.683 kb on - strand at 2861.715 kb on + strand at 2861.952 kb on - strand at 2862.136 kb on - strand at 2862.257 kb on - strand, within BT2282 at 2862.270 kb on - strand, within BT2282 at 2862.346 kb on - strand, within BT2282 at 2862.385 kb on - strand, within BT2282 at 2862.385 kb on - strand, within BT2282
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.025 mM remove 2,860,156 - BT2279 0.52 -1.9 2,860,161 + BT2279 0.53 -1.5 2,860,466 + BT2279 0.86 +1.6 2,860,479 + BT2279 0.88 -1.9 2,860,479 + BT2279 0.88 -2.8 2,860,668 + BT2280 0.19 +2.1 2,860,668 + BT2280 0.19 -0.5 2,860,669 - BT2280 0.20 -0.7 2,860,810 - BT2280 0.57 -1.5 2,860,856 - BT2280 0.70 +0.1 2,860,882 - BT2280 0.77 -0.5 2,860,912 + BT2280 0.85 -1.3 2,860,979 + -0.5 2,860,980 - -2.9 2,860,980 - -0.5 2,860,980 - +1.4 2,860,982 + -0.8 2,860,982 + -3.2 2,861,036 + +1.8 2,861,059 + -1.9 2,861,093 + +0.1 2,861,093 + -2.1 2,861,094 - -0.9 2,861,094 - +1.5 2,861,142 - -0.7 2,861,142 - -2.6 2,861,182 + -1.0 2,861,182 + -1.5 2,861,186 + BT2281 0.11 +1.6 2,861,197 - BT2281 0.15 -0.9 2,861,304 - BT2281 0.54 +1.7 2,861,342 - BT2281 0.68 +0.1 2,861,367 + BT2281 0.77 +0.1 2,861,367 + BT2281 0.77 +7.0 2,861,428 + -3.8 2,861,440 + -0.8 2,861,441 - -1.8 2,861,444 - -2.3 2,861,448 + +0.5 2,861,521 + -1.2 2,861,536 - -1.0 2,861,536 - +0.1 2,861,536 - -1.5 2,861,536 - -0.5 2,861,536 - -0.9 2,861,561 + -1.9 2,861,617 - -1.5 2,861,617 - -2.3 2,861,668 - -1.5 2,861,668 - -3.9 2,861,671 + +1.1 2,861,683 - -1.5 2,861,715 + +0.1 2,861,952 - -1.9 2,862,136 - -2.4 2,862,257 - BT2282 0.12 +2.9 2,862,270 - BT2282 0.15 -1.4 2,862,346 - BT2282 0.28 +0.1 2,862,385 - BT2282 0.35 +1.9 2,862,385 - BT2282 0.35 +0.1
Or see this region's nucleotide sequence