Experiment: BHIS with Thioridazine 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1518 and BT1519 are separated by 13 nucleotides BT1519 and BT1520 are separated by 209 nucleotides BT1520 and BT1521 are separated by 2 nucleotides BT1521 and BT1522 are separated by 56 nucleotides
BT1518: BT1518 - hypothetical protein (NCBI ptt file), at 1,874,996 to 1,875,301
BT1518
BT1519: BT1519 - N-acetylmuramoyl-L-alanine amidase (NCBI ptt file), at 1,875,315 to 1,875,761
BT1519
BT1520: BT1520 - putative transcriptional regulator (NCBI ptt file), at 1,875,971 to 1,876,249
BT1520
BT1521: BT1521 - conserved hypothetical protein (NCBI ptt file), at 1,876,252 to 1,877,181
BT1521
BT1522: BT1522 - putative aureobasidin A resistance protein (NCBI ptt file), at 1,877,238 to 1,878,155
BT1522
Position (kb)
1875
1876
1877 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1874.982 kb on - strand at 1875.020 kb on - strand at 1875.023 kb on - strand at 1875.053 kb on - strand, within BT1518 at 1875.061 kb on + strand, within BT1518 at 1875.116 kb on - strand, within BT1518 at 1875.116 kb on - strand, within BT1518 at 1875.116 kb on - strand, within BT1518 at 1875.144 kb on + strand, within BT1518 at 1875.237 kb on + strand, within BT1518 at 1875.246 kb on + strand, within BT1518 at 1875.269 kb on - strand, within BT1518 at 1875.381 kb on + strand, within BT1519 at 1875.458 kb on + strand, within BT1519 at 1875.460 kb on + strand, within BT1519 at 1875.460 kb on + strand, within BT1519 at 1875.528 kb on - strand, within BT1519 at 1875.617 kb on + strand, within BT1519 at 1875.618 kb on - strand, within BT1519 at 1875.797 kb on - strand at 1875.798 kb on + strand at 1875.854 kb on - strand at 1875.911 kb on + strand at 1875.915 kb on - strand at 1875.916 kb on + strand at 1875.934 kb on + strand at 1875.935 kb on - strand at 1875.946 kb on - strand at 1875.946 kb on - strand at 1876.021 kb on - strand, within BT1520 at 1876.063 kb on + strand, within BT1520 at 1876.137 kb on + strand, within BT1520 at 1876.141 kb on + strand, within BT1520 at 1876.156 kb on + strand, within BT1520 at 1876.179 kb on + strand, within BT1520 at 1876.261 kb on - strand at 1876.545 kb on - strand, within BT1521 at 1876.609 kb on - strand, within BT1521 at 1876.613 kb on - strand, within BT1521 at 1876.722 kb on - strand, within BT1521 at 1876.740 kb on + strand, within BT1521 at 1876.740 kb on + strand, within BT1521 at 1876.744 kb on + strand, within BT1521 at 1876.744 kb on + strand, within BT1521 at 1876.745 kb on - strand, within BT1521 at 1876.798 kb on + strand, within BT1521 at 1876.817 kb on + strand, within BT1521 at 1876.817 kb on + strand, within BT1521 at 1876.875 kb on - strand, within BT1521 at 1876.909 kb on + strand, within BT1521 at 1876.909 kb on + strand, within BT1521 at 1876.910 kb on - strand, within BT1521 at 1876.911 kb on + strand, within BT1521 at 1876.999 kb on - strand, within BT1521 at 1877.005 kb on - strand, within BT1521 at 1877.007 kb on - strand, within BT1521 at 1877.047 kb on + strand, within BT1521 at 1877.047 kb on + strand, within BT1521 at 1877.057 kb on + strand, within BT1521 at 1877.070 kb on + strand, within BT1521 at 1877.071 kb on - strand, within BT1521 at 1877.218 kb on - strand at 1877.240 kb on - strand at 1877.244 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.025 mM remove 1,874,982 - -1.8 1,875,020 - -3.3 1,875,023 - +0.8 1,875,053 - BT1518 0.19 -1.0 1,875,061 + BT1518 0.21 +0.2 1,875,116 - BT1518 0.39 -1.6 1,875,116 - BT1518 0.39 +0.3 1,875,116 - BT1518 0.39 +1.7 1,875,144 + BT1518 0.48 +1.8 1,875,237 + BT1518 0.79 -3.2 1,875,246 + BT1518 0.82 -0.2 1,875,269 - BT1518 0.89 +0.4 1,875,381 + BT1519 0.15 -0.5 1,875,458 + BT1519 0.32 +0.5 1,875,460 + BT1519 0.32 +1.4 1,875,460 + BT1519 0.32 -4.0 1,875,528 - BT1519 0.48 -2.7 1,875,617 + BT1519 0.68 +3.0 1,875,618 - BT1519 0.68 -1.2 1,875,797 - -1.0 1,875,798 + -2.2 1,875,854 - -1.2 1,875,911 + -1.2 1,875,915 - -2.3 1,875,916 + +0.4 1,875,934 + -1.0 1,875,935 - -1.5 1,875,946 - -0.6 1,875,946 - +0.2 1,876,021 - BT1520 0.18 +2.6 1,876,063 + BT1520 0.33 -0.8 1,876,137 + BT1520 0.59 -1.8 1,876,141 + BT1520 0.61 +2.3 1,876,156 + BT1520 0.66 -2.4 1,876,179 + BT1520 0.75 +2.6 1,876,261 - -2.9 1,876,545 - BT1521 0.32 -1.7 1,876,609 - BT1521 0.38 +0.4 1,876,613 - BT1521 0.39 -0.3 1,876,722 - BT1521 0.51 -1.2 1,876,740 + BT1521 0.52 -1.6 1,876,740 + BT1521 0.52 +0.7 1,876,744 + BT1521 0.53 -1.2 1,876,744 + BT1521 0.53 -0.7 1,876,745 - BT1521 0.53 +2.2 1,876,798 + BT1521 0.59 -2.5 1,876,817 + BT1521 0.61 -4.3 1,876,817 + BT1521 0.61 -2.1 1,876,875 - BT1521 0.67 -3.3 1,876,909 + BT1521 0.71 +0.8 1,876,909 + BT1521 0.71 -4.1 1,876,910 - BT1521 0.71 -0.2 1,876,911 + BT1521 0.71 -2.3 1,876,999 - BT1521 0.80 -1.9 1,877,005 - BT1521 0.81 -1.0 1,877,007 - BT1521 0.81 -0.6 1,877,047 + BT1521 0.85 -1.6 1,877,047 + BT1521 0.85 -0.7 1,877,057 + BT1521 0.87 +1.6 1,877,070 + BT1521 0.88 +0.0 1,877,071 - BT1521 0.88 -0.2 1,877,218 - -2.1 1,877,240 - +2.1 1,877,244 - +0.8
Or see this region's nucleotide sequence