Experiment: BHIS with Thioridazine 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1313 and BT1314 are separated by 18 nucleotides BT1314 and BT1315 are separated by 494 nucleotides
BT1313: BT1313 - muconate cycloisomerase (NCBI ptt file), at 1,637,748 to 1,638,899
BT1313
BT1314: BT1314 - dipeptidyl-peptidase VI (NCBI ptt file), at 1,638,918 to 1,639,904
BT1314
BT1315: BT1315 - ribonuclease BN (NCBI ptt file), at 1,640,399 to 1,641,718
BT1315
Position (kb)
1638
1639
1640 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1637.922 kb on - strand, within BT1313 at 1638.046 kb on - strand, within BT1313 at 1638.314 kb on + strand, within BT1313 at 1638.365 kb on + strand, within BT1313 at 1638.425 kb on + strand, within BT1313 at 1638.620 kb on - strand, within BT1313 at 1638.630 kb on - strand, within BT1313 at 1638.661 kb on + strand, within BT1313 at 1638.693 kb on - strand, within BT1313 at 1638.735 kb on + strand, within BT1313 at 1638.781 kb on + strand, within BT1313 at 1638.971 kb on + strand at 1638.976 kb on - strand at 1638.979 kb on + strand at 1639.066 kb on - strand, within BT1314 at 1639.105 kb on + strand, within BT1314 at 1639.133 kb on + strand, within BT1314 at 1639.203 kb on + strand, within BT1314 at 1639.221 kb on + strand, within BT1314 at 1639.225 kb on + strand, within BT1314 at 1639.260 kb on - strand, within BT1314 at 1639.334 kb on - strand, within BT1314 at 1639.424 kb on + strand, within BT1314 at 1639.438 kb on + strand, within BT1314 at 1639.571 kb on - strand, within BT1314 at 1639.637 kb on + strand, within BT1314 at 1639.711 kb on - strand, within BT1314 at 1639.757 kb on + strand, within BT1314 at 1639.798 kb on - strand, within BT1314 at 1639.864 kb on - strand at 1639.917 kb on + strand at 1639.918 kb on - strand at 1640.473 kb on + strand at 1640.503 kb on + strand at 1640.555 kb on - strand, within BT1315 at 1640.574 kb on - strand, within BT1315 at 1640.576 kb on - strand, within BT1315 at 1640.595 kb on + strand, within BT1315 at 1640.644 kb on + strand, within BT1315 at 1640.807 kb on - strand, within BT1315 at 1640.880 kb on - strand, within BT1315
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.025 mM remove 1,637,922 - BT1313 0.15 -0.1 1,638,046 - BT1313 0.26 -0.1 1,638,314 + BT1313 0.49 -0.5 1,638,365 + BT1313 0.54 +1.9 1,638,425 + BT1313 0.59 +1.3 1,638,620 - BT1313 0.76 -1.1 1,638,630 - BT1313 0.77 +1.4 1,638,661 + BT1313 0.79 +2.3 1,638,693 - BT1313 0.82 -1.1 1,638,735 + BT1313 0.86 -1.7 1,638,781 + BT1313 0.90 -0.8 1,638,971 + +0.9 1,638,976 - -0.1 1,638,979 + +0.4 1,639,066 - BT1314 0.15 -0.7 1,639,105 + BT1314 0.19 -2.9 1,639,133 + BT1314 0.22 -1.4 1,639,203 + BT1314 0.29 -0.1 1,639,221 + BT1314 0.31 -0.1 1,639,225 + BT1314 0.31 +2.9 1,639,260 - BT1314 0.35 +0.9 1,639,334 - BT1314 0.42 -2.7 1,639,424 + BT1314 0.51 +2.5 1,639,438 + BT1314 0.53 +1.7 1,639,571 - BT1314 0.66 -0.1 1,639,637 + BT1314 0.73 +1.2 1,639,711 - BT1314 0.80 +0.9 1,639,757 + BT1314 0.85 -1.1 1,639,798 - BT1314 0.89 +1.2 1,639,864 - +0.3 1,639,917 + -0.1 1,639,918 - +0.6 1,640,473 + -5.2 1,640,503 + -3.9 1,640,555 - BT1315 0.12 -1.1 1,640,574 - BT1315 0.13 -1.7 1,640,576 - BT1315 0.13 +0.5 1,640,595 + BT1315 0.15 -3.1 1,640,644 + BT1315 0.19 -2.7 1,640,807 - BT1315 0.31 -2.1 1,640,880 - BT1315 0.36 -2.5
Or see this region's nucleotide sequence