Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT1217

Experiment: BHIS with Thioridazine 0.025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT1216 and BT1217 are separated by 70 nucleotidesBT1217 and BT1218 are separated by 421 nucleotides BT1216: BT1216 - putative two-component system sensor protein, without kinase domain (NCBI ptt file), at 1,513,116 to 1,514,189 BT1216 BT1217: BT1217 - two-component system response regulator (NCBI ptt file), at 1,514,260 to 1,514,958 BT1217 BT1218: BT1218 - conserved hypothetical protein, with conserved domain (NCBI ptt file), at 1,515,380 to 1,516,078 BT1218 Position (kb) 1514 1515Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1513.349 kb on + strand, within BT1216at 1513.350 kb on - strand, within BT1216at 1513.353 kb on - strand, within BT1216at 1513.362 kb on - strand, within BT1216at 1513.528 kb on + strand, within BT1216at 1513.659 kb on - strand, within BT1216at 1513.659 kb on - strand, within BT1216at 1513.659 kb on - strand, within BT1216at 1513.659 kb on - strand, within BT1216at 1513.715 kb on - strand, within BT1216at 1513.759 kb on - strand, within BT1216at 1513.798 kb on - strand, within BT1216at 1513.899 kb on - strand, within BT1216at 1514.104 kb on - strandat 1514.181 kb on - strandat 1514.188 kb on - strandat 1514.190 kb on + strandat 1514.201 kb on + strandat 1514.250 kb on - strandat 1514.259 kb on + strandat 1514.267 kb on + strandat 1514.267 kb on + strandat 1514.570 kb on - strand, within BT1217at 1514.666 kb on - strand, within BT1217at 1514.682 kb on - strand, within BT1217at 1514.702 kb on - strand, within BT1217at 1514.732 kb on + strand, within BT1217at 1514.736 kb on + strand, within BT1217at 1514.816 kb on + strand, within BT1217at 1514.877 kb on + strand, within BT1217at 1514.897 kb on - strandat 1514.908 kb on - strandat 1514.913 kb on + strandat 1515.023 kb on + strandat 1515.023 kb on + strandat 1515.024 kb on - strandat 1515.024 kb on - strandat 1515.026 kb on - strandat 1515.072 kb on - strandat 1515.091 kb on + strandat 1515.158 kb on - strandat 1515.164 kb on - strandat 1515.224 kb on - strandat 1515.241 kb on + strandat 1515.334 kb on + strandat 1515.335 kb on - strandat 1515.336 kb on + strandat 1515.337 kb on - strandat 1515.341 kb on - strandat 1515.366 kb on + strandat 1515.367 kb on - strandat 1515.379 kb on + strandat 1515.485 kb on + strand, within BT1218at 1515.520 kb on + strand, within BT1218at 1515.544 kb on - strand, within BT1218at 1515.607 kb on - strand, within BT1218at 1515.741 kb on + strand, within BT1218at 1515.749 kb on + strand, within BT1218at 1515.749 kb on + strand, within BT1218at 1515.749 kb on + strand, within BT1218at 1515.750 kb on - strand, within BT1218at 1515.751 kb on + strand, within BT1218at 1515.751 kb on + strand, within BT1218at 1515.752 kb on - strand, within BT1218at 1515.753 kb on + strand, within BT1218at 1515.753 kb on + strand, within BT1218at 1515.753 kb on + strand, within BT1218at 1515.794 kb on + strand, within BT1218

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.025 mM
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1,513,349 + BT1216 0.22 -1.2
1,513,350 - BT1216 0.22 -1.2
1,513,353 - BT1216 0.22 +0.8
1,513,362 - BT1216 0.23 +0.8
1,513,528 + BT1216 0.38 -1.7
1,513,659 - BT1216 0.51 -4.5
1,513,659 - BT1216 0.51 -1.7
1,513,659 - BT1216 0.51 -0.2
1,513,659 - BT1216 0.51 +1.8
1,513,715 - BT1216 0.56 -0.7
1,513,759 - BT1216 0.60 -0.2
1,513,798 - BT1216 0.64 -0.9
1,513,899 - BT1216 0.73 -0.5
1,514,104 - -3.6
1,514,181 - -1.1
1,514,188 - -0.2
1,514,190 + -1.6
1,514,201 + +0.4
1,514,250 - +0.8
1,514,259 + -0.2
1,514,267 + -0.2
1,514,267 + -1.8
1,514,570 - BT1217 0.44 +2.2
1,514,666 - BT1217 0.58 -0.2
1,514,682 - BT1217 0.60 +1.7
1,514,702 - BT1217 0.63 +1.2
1,514,732 + BT1217 0.68 +1.6
1,514,736 + BT1217 0.68 +1.7
1,514,816 + BT1217 0.80 +0.8
1,514,877 + BT1217 0.88 +1.0
1,514,897 - -1.6
1,514,908 - +0.6
1,514,913 + -3.3
1,515,023 + -1.2
1,515,023 + +1.7
1,515,024 - -2.5
1,515,024 - -1.2
1,515,026 - -1.7
1,515,072 - -0.2
1,515,091 + -0.2
1,515,158 - -1.2
1,515,164 - +1.4
1,515,224 - +1.9
1,515,241 + +0.7
1,515,334 + +1.2
1,515,335 - -1.2
1,515,336 + -1.7
1,515,337 - +0.4
1,515,341 - +0.8
1,515,366 + +0.5
1,515,367 - -1.2
1,515,379 + -1.2
1,515,485 + BT1218 0.15 -1.2
1,515,520 + BT1218 0.20 +2.3
1,515,544 - BT1218 0.23 -2.9
1,515,607 - BT1218 0.32 +2.2
1,515,741 + BT1218 0.52 -2.1
1,515,749 + BT1218 0.53 +1.4
1,515,749 + BT1218 0.53 -1.2
1,515,749 + BT1218 0.53 -3.9
1,515,750 - BT1218 0.53 +0.8
1,515,751 + BT1218 0.53 -0.9
1,515,751 + BT1218 0.53 -1.7
1,515,752 - BT1218 0.53 +0.7
1,515,753 + BT1218 0.53 +1.0
1,515,753 + BT1218 0.53 -0.3
1,515,753 + BT1218 0.53 +0.2
1,515,794 + BT1218 0.59 -3.3

Or see this region's nucleotide sequence