Experiment: BHIS with Thioridazine 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1216 and BT1217 are separated by 70 nucleotides BT1217 and BT1218 are separated by 421 nucleotides
BT1216: BT1216 - putative two-component system sensor protein, without kinase domain (NCBI ptt file), at 1,513,116 to 1,514,189
BT1216
BT1217: BT1217 - two-component system response regulator (NCBI ptt file), at 1,514,260 to 1,514,958
BT1217
BT1218: BT1218 - conserved hypothetical protein, with conserved domain (NCBI ptt file), at 1,515,380 to 1,516,078
BT1218
Position (kb)
1514
1515 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1513.349 kb on + strand, within BT1216 at 1513.350 kb on - strand, within BT1216 at 1513.353 kb on - strand, within BT1216 at 1513.362 kb on - strand, within BT1216 at 1513.528 kb on + strand, within BT1216 at 1513.659 kb on - strand, within BT1216 at 1513.659 kb on - strand, within BT1216 at 1513.659 kb on - strand, within BT1216 at 1513.659 kb on - strand, within BT1216 at 1513.715 kb on - strand, within BT1216 at 1513.759 kb on - strand, within BT1216 at 1513.798 kb on - strand, within BT1216 at 1513.899 kb on - strand, within BT1216 at 1514.104 kb on - strand at 1514.181 kb on - strand at 1514.188 kb on - strand at 1514.190 kb on + strand at 1514.201 kb on + strand at 1514.250 kb on - strand at 1514.259 kb on + strand at 1514.267 kb on + strand at 1514.267 kb on + strand at 1514.570 kb on - strand, within BT1217 at 1514.666 kb on - strand, within BT1217 at 1514.682 kb on - strand, within BT1217 at 1514.702 kb on - strand, within BT1217 at 1514.732 kb on + strand, within BT1217 at 1514.736 kb on + strand, within BT1217 at 1514.816 kb on + strand, within BT1217 at 1514.877 kb on + strand, within BT1217 at 1514.897 kb on - strand at 1514.908 kb on - strand at 1514.913 kb on + strand at 1515.023 kb on + strand at 1515.023 kb on + strand at 1515.024 kb on - strand at 1515.024 kb on - strand at 1515.026 kb on - strand at 1515.072 kb on - strand at 1515.091 kb on + strand at 1515.158 kb on - strand at 1515.164 kb on - strand at 1515.224 kb on - strand at 1515.241 kb on + strand at 1515.334 kb on + strand at 1515.335 kb on - strand at 1515.336 kb on + strand at 1515.337 kb on - strand at 1515.341 kb on - strand at 1515.366 kb on + strand at 1515.367 kb on - strand at 1515.379 kb on + strand at 1515.485 kb on + strand, within BT1218 at 1515.520 kb on + strand, within BT1218 at 1515.544 kb on - strand, within BT1218 at 1515.607 kb on - strand, within BT1218 at 1515.741 kb on + strand, within BT1218 at 1515.749 kb on + strand, within BT1218 at 1515.749 kb on + strand, within BT1218 at 1515.749 kb on + strand, within BT1218 at 1515.750 kb on - strand, within BT1218 at 1515.751 kb on + strand, within BT1218 at 1515.751 kb on + strand, within BT1218 at 1515.752 kb on - strand, within BT1218 at 1515.753 kb on + strand, within BT1218 at 1515.753 kb on + strand, within BT1218 at 1515.753 kb on + strand, within BT1218 at 1515.794 kb on + strand, within BT1218
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.025 mM remove 1,513,349 + BT1216 0.22 -1.2 1,513,350 - BT1216 0.22 -1.2 1,513,353 - BT1216 0.22 +0.8 1,513,362 - BT1216 0.23 +0.8 1,513,528 + BT1216 0.38 -1.7 1,513,659 - BT1216 0.51 -4.5 1,513,659 - BT1216 0.51 -1.7 1,513,659 - BT1216 0.51 -0.2 1,513,659 - BT1216 0.51 +1.8 1,513,715 - BT1216 0.56 -0.7 1,513,759 - BT1216 0.60 -0.2 1,513,798 - BT1216 0.64 -0.9 1,513,899 - BT1216 0.73 -0.5 1,514,104 - -3.6 1,514,181 - -1.1 1,514,188 - -0.2 1,514,190 + -1.6 1,514,201 + +0.4 1,514,250 - +0.8 1,514,259 + -0.2 1,514,267 + -0.2 1,514,267 + -1.8 1,514,570 - BT1217 0.44 +2.2 1,514,666 - BT1217 0.58 -0.2 1,514,682 - BT1217 0.60 +1.7 1,514,702 - BT1217 0.63 +1.2 1,514,732 + BT1217 0.68 +1.6 1,514,736 + BT1217 0.68 +1.7 1,514,816 + BT1217 0.80 +0.8 1,514,877 + BT1217 0.88 +1.0 1,514,897 - -1.6 1,514,908 - +0.6 1,514,913 + -3.3 1,515,023 + -1.2 1,515,023 + +1.7 1,515,024 - -2.5 1,515,024 - -1.2 1,515,026 - -1.7 1,515,072 - -0.2 1,515,091 + -0.2 1,515,158 - -1.2 1,515,164 - +1.4 1,515,224 - +1.9 1,515,241 + +0.7 1,515,334 + +1.2 1,515,335 - -1.2 1,515,336 + -1.7 1,515,337 - +0.4 1,515,341 - +0.8 1,515,366 + +0.5 1,515,367 - -1.2 1,515,379 + -1.2 1,515,485 + BT1218 0.15 -1.2 1,515,520 + BT1218 0.20 +2.3 1,515,544 - BT1218 0.23 -2.9 1,515,607 - BT1218 0.32 +2.2 1,515,741 + BT1218 0.52 -2.1 1,515,749 + BT1218 0.53 +1.4 1,515,749 + BT1218 0.53 -1.2 1,515,749 + BT1218 0.53 -3.9 1,515,750 - BT1218 0.53 +0.8 1,515,751 + BT1218 0.53 -0.9 1,515,751 + BT1218 0.53 -1.7 1,515,752 - BT1218 0.53 +0.7 1,515,753 + BT1218 0.53 +1.0 1,515,753 + BT1218 0.53 -0.3 1,515,753 + BT1218 0.53 +0.2 1,515,794 + BT1218 0.59 -3.3
Or see this region's nucleotide sequence