Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_983 and Pf6N2E2_984 are separated by 232 nucleotides Pf6N2E2_984 and Pf6N2E2_985 are separated by 217 nucleotides Pf6N2E2_985 and Pf6N2E2_986 are separated by 315 nucleotides Pf6N2E2_986 and Pf6N2E2_987 overlap by 4 nucleotides Pf6N2E2_987 and Pf6N2E2_988 are separated by 247 nucleotides
Pf6N2E2_983 - Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases, at 1,151,989 to 1,152,981
_983
Pf6N2E2_984 - FIG00553873: hypothetical protein, at 1,153,214 to 1,153,591
_984
Pf6N2E2_985 - Multidrug resistance transporter, Bcr/CflA family, at 1,153,809 to 1,154,945
_985
Pf6N2E2_986 - hypothetical protein, at 1,155,261 to 1,155,383
_986
Pf6N2E2_987 - hypothetical protein, at 1,155,380 to 1,155,616
_987
Pf6N2E2_988 - 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27), at 1,155,864 to 1,156,721
_988
Position (kb)
1153
1154
1155 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1152.917 kb on + strand at 1152.917 kb on + strand at 1152.917 kb on + strand at 1152.917 kb on + strand at 1152.917 kb on + strand at 1152.917 kb on + strand at 1152.918 kb on - strand at 1152.966 kb on + strand at 1152.966 kb on + strand at 1152.995 kb on + strand at 1153.008 kb on - strand at 1153.130 kb on + strand at 1153.162 kb on + strand at 1153.162 kb on + strand at 1153.162 kb on + strand at 1153.191 kb on - strand at 1153.261 kb on + strand, within Pf6N2E2_984 at 1153.262 kb on - strand, within Pf6N2E2_984 at 1153.379 kb on + strand, within Pf6N2E2_984 at 1153.380 kb on - strand, within Pf6N2E2_984 at 1153.398 kb on + strand, within Pf6N2E2_984 at 1153.398 kb on + strand, within Pf6N2E2_984 at 1153.399 kb on - strand, within Pf6N2E2_984 at 1153.528 kb on - strand, within Pf6N2E2_984 at 1153.528 kb on - strand, within Pf6N2E2_984 at 1153.528 kb on - strand, within Pf6N2E2_984 at 1153.528 kb on - strand, within Pf6N2E2_984 at 1153.528 kb on - strand, within Pf6N2E2_984 at 1153.528 kb on - strand, within Pf6N2E2_984 at 1153.533 kb on + strand, within Pf6N2E2_984 at 1153.533 kb on + strand, within Pf6N2E2_984 at 1153.534 kb on - strand, within Pf6N2E2_984 at 1153.534 kb on - strand, within Pf6N2E2_984 at 1153.633 kb on - strand at 1153.633 kb on - strand at 1153.633 kb on - strand at 1153.786 kb on + strand at 1153.786 kb on + strand at 1153.786 kb on + strand at 1153.788 kb on + strand at 1153.789 kb on - strand at 1153.789 kb on - strand at 1153.789 kb on - strand at 1153.933 kb on + strand, within Pf6N2E2_985 at 1153.933 kb on + strand, within Pf6N2E2_985 at 1153.934 kb on - strand, within Pf6N2E2_985 at 1153.934 kb on - strand, within Pf6N2E2_985 at 1153.934 kb on - strand, within Pf6N2E2_985 at 1153.990 kb on - strand, within Pf6N2E2_985 at 1153.990 kb on - strand, within Pf6N2E2_985 at 1153.990 kb on - strand, within Pf6N2E2_985 at 1153.990 kb on - strand, within Pf6N2E2_985 at 1153.992 kb on + strand, within Pf6N2E2_985 at 1154.017 kb on - strand, within Pf6N2E2_985 at 1154.017 kb on - strand, within Pf6N2E2_985 at 1154.018 kb on + strand, within Pf6N2E2_985 at 1154.018 kb on + strand, within Pf6N2E2_985 at 1154.019 kb on - strand, within Pf6N2E2_985 at 1154.019 kb on - strand, within Pf6N2E2_985 at 1154.019 kb on - strand, within Pf6N2E2_985 at 1154.022 kb on + strand, within Pf6N2E2_985 at 1154.023 kb on - strand, within Pf6N2E2_985 at 1154.023 kb on - strand, within Pf6N2E2_985 at 1154.023 kb on - strand, within Pf6N2E2_985 at 1154.023 kb on - strand, within Pf6N2E2_985 at 1154.023 kb on - strand, within Pf6N2E2_985 at 1154.023 kb on - strand, within Pf6N2E2_985 at 1154.023 kb on - strand, within Pf6N2E2_985 at 1154.100 kb on - strand, within Pf6N2E2_985 at 1154.108 kb on - strand, within Pf6N2E2_985 at 1154.108 kb on - strand, within Pf6N2E2_985 at 1154.108 kb on - strand, within Pf6N2E2_985 at 1154.108 kb on - strand, within Pf6N2E2_985 at 1154.214 kb on + strand, within Pf6N2E2_985 at 1154.272 kb on - strand, within Pf6N2E2_985 at 1154.337 kb on - strand, within Pf6N2E2_985 at 1154.339 kb on - strand, within Pf6N2E2_985 at 1154.339 kb on - strand, within Pf6N2E2_985 at 1154.348 kb on + strand, within Pf6N2E2_985 at 1154.348 kb on + strand, within Pf6N2E2_985 at 1154.348 kb on + strand, within Pf6N2E2_985 at 1154.349 kb on - strand, within Pf6N2E2_985 at 1154.416 kb on + strand, within Pf6N2E2_985 at 1154.416 kb on + strand, within Pf6N2E2_985 at 1154.511 kb on + strand, within Pf6N2E2_985 at 1154.512 kb on - strand, within Pf6N2E2_985 at 1154.527 kb on + strand, within Pf6N2E2_985 at 1154.527 kb on + strand, within Pf6N2E2_985 at 1154.527 kb on + strand, within Pf6N2E2_985 at 1154.528 kb on - strand, within Pf6N2E2_985 at 1154.544 kb on - strand, within Pf6N2E2_985 at 1154.673 kb on + strand, within Pf6N2E2_985 at 1154.706 kb on + strand, within Pf6N2E2_985 at 1154.707 kb on - strand, within Pf6N2E2_985 at 1154.865 kb on - strand at 1155.037 kb on + strand at 1155.038 kb on - strand at 1155.050 kb on + strand at 1155.122 kb on + strand at 1155.122 kb on + strand at 1155.122 kb on + strand at 1155.123 kb on - strand at 1155.141 kb on + strand at 1155.172 kb on + strand at 1155.173 kb on - strand at 1155.299 kb on + strand, within Pf6N2E2_986 at 1155.493 kb on + strand, within Pf6N2E2_987 at 1155.500 kb on + strand, within Pf6N2E2_987 at 1155.500 kb on + strand, within Pf6N2E2_987 at 1155.541 kb on - strand, within Pf6N2E2_987 at 1155.541 kb on - strand, within Pf6N2E2_987 at 1155.603 kb on + strand at 1155.604 kb on - strand at 1155.803 kb on + strand at 1155.865 kb on + strand at 1155.865 kb on + strand at 1155.865 kb on + strand at 1155.865 kb on + strand at 1155.866 kb on - strand at 1155.866 kb on - strand at 1155.866 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 1,152,917 + -2.3 1,152,917 + -0.7 1,152,917 + -3.0 1,152,917 + -0.5 1,152,917 + +0.1 1,152,917 + -1.6 1,152,918 - +1.5 1,152,966 + -0.2 1,152,966 + +1.5 1,152,995 + +1.1 1,153,008 - +2.1 1,153,130 + +0.3 1,153,162 + -0.8 1,153,162 + -0.3 1,153,162 + +0.4 1,153,191 - +0.1 1,153,261 + Pf6N2E2_984 0.12 -1.2 1,153,262 - Pf6N2E2_984 0.13 -0.5 1,153,379 + Pf6N2E2_984 0.44 +0.9 1,153,380 - Pf6N2E2_984 0.44 -1.4 1,153,398 + Pf6N2E2_984 0.49 +0.0 1,153,398 + Pf6N2E2_984 0.49 +1.0 1,153,399 - Pf6N2E2_984 0.49 +0.5 1,153,528 - Pf6N2E2_984 0.83 +0.3 1,153,528 - Pf6N2E2_984 0.83 +0.5 1,153,528 - Pf6N2E2_984 0.83 -0.1 1,153,528 - Pf6N2E2_984 0.83 +1.8 1,153,528 - Pf6N2E2_984 0.83 -0.5 1,153,528 - Pf6N2E2_984 0.83 -0.5 1,153,533 + Pf6N2E2_984 0.84 +2.1 1,153,533 + Pf6N2E2_984 0.84 -0.0 1,153,534 - Pf6N2E2_984 0.85 +0.3 1,153,534 - Pf6N2E2_984 0.85 -0.7 1,153,633 - +1.8 1,153,633 - -0.9 1,153,633 - -1.8 1,153,786 + +1.8 1,153,786 + -0.4 1,153,786 + -1.2 1,153,788 + +0.8 1,153,789 - -2.0 1,153,789 - -2.3 1,153,789 - +0.7 1,153,933 + Pf6N2E2_985 0.11 -2.0 1,153,933 + Pf6N2E2_985 0.11 +1.3 1,153,934 - Pf6N2E2_985 0.11 -0.5 1,153,934 - Pf6N2E2_985 0.11 +1.4 1,153,934 - Pf6N2E2_985 0.11 +0.8 1,153,990 - Pf6N2E2_985 0.16 +0.8 1,153,990 - Pf6N2E2_985 0.16 +0.1 1,153,990 - Pf6N2E2_985 0.16 +3.4 1,153,990 - Pf6N2E2_985 0.16 +1.2 1,153,992 + Pf6N2E2_985 0.16 +1.6 1,154,017 - Pf6N2E2_985 0.18 +1.8 1,154,017 - Pf6N2E2_985 0.18 -0.1 1,154,018 + Pf6N2E2_985 0.18 +0.6 1,154,018 + Pf6N2E2_985 0.18 +0.4 1,154,019 - Pf6N2E2_985 0.18 -0.4 1,154,019 - Pf6N2E2_985 0.18 -0.4 1,154,019 - Pf6N2E2_985 0.18 +1.7 1,154,022 + Pf6N2E2_985 0.19 +1.1 1,154,023 - Pf6N2E2_985 0.19 +1.4 1,154,023 - Pf6N2E2_985 0.19 -0.5 1,154,023 - Pf6N2E2_985 0.19 -0.7 1,154,023 - Pf6N2E2_985 0.19 -0.4 1,154,023 - Pf6N2E2_985 0.19 -0.4 1,154,023 - Pf6N2E2_985 0.19 -0.3 1,154,023 - Pf6N2E2_985 0.19 +1.8 1,154,100 - Pf6N2E2_985 0.26 +1.9 1,154,108 - Pf6N2E2_985 0.26 -0.1 1,154,108 - Pf6N2E2_985 0.26 +0.1 1,154,108 - Pf6N2E2_985 0.26 +1.8 1,154,108 - Pf6N2E2_985 0.26 -1.1 1,154,214 + Pf6N2E2_985 0.36 -2.4 1,154,272 - Pf6N2E2_985 0.41 +1.9 1,154,337 - Pf6N2E2_985 0.46 +0.7 1,154,339 - Pf6N2E2_985 0.47 +2.8 1,154,339 - Pf6N2E2_985 0.47 +1.5 1,154,348 + Pf6N2E2_985 0.47 +1.5 1,154,348 + Pf6N2E2_985 0.47 -0.7 1,154,348 + Pf6N2E2_985 0.47 +0.6 1,154,349 - Pf6N2E2_985 0.47 +0.8 1,154,416 + Pf6N2E2_985 0.53 -1.4 1,154,416 + Pf6N2E2_985 0.53 +0.2 1,154,511 + Pf6N2E2_985 0.62 -1.0 1,154,512 - Pf6N2E2_985 0.62 -0.3 1,154,527 + Pf6N2E2_985 0.63 -2.4 1,154,527 + Pf6N2E2_985 0.63 -1.2 1,154,527 + Pf6N2E2_985 0.63 +1.7 1,154,528 - Pf6N2E2_985 0.63 +1.8 1,154,544 - Pf6N2E2_985 0.65 -2.6 1,154,673 + Pf6N2E2_985 0.76 +3.2 1,154,706 + Pf6N2E2_985 0.79 +0.1 1,154,707 - Pf6N2E2_985 0.79 +0.7 1,154,865 - -0.1 1,155,037 + +0.4 1,155,038 - -0.8 1,155,050 + +1.5 1,155,122 + +0.8 1,155,122 + +0.8 1,155,122 + -1.9 1,155,123 - +0.8 1,155,141 + +1.8 1,155,172 + -0.4 1,155,173 - +0.8 1,155,299 + Pf6N2E2_986 0.31 -0.8 1,155,493 + Pf6N2E2_987 0.48 +0.8 1,155,500 + Pf6N2E2_987 0.51 -0.2 1,155,500 + Pf6N2E2_987 0.51 +1.0 1,155,541 - Pf6N2E2_987 0.68 -0.2 1,155,541 - Pf6N2E2_987 0.68 +1.4 1,155,603 + -0.1 1,155,604 - -0.2 1,155,803 + -1.1 1,155,865 + +0.7 1,155,865 + -0.4 1,155,865 + -2.1 1,155,865 + +0.7 1,155,866 - +0.4 1,155,866 - -2.6 1,155,866 - -0.5
Or see this region's nucleotide sequence