Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_628 and Pf6N2E2_629 are separated by 92 nucleotides Pf6N2E2_629 and Pf6N2E2_630 overlap by 8 nucleotides Pf6N2E2_630 and Pf6N2E2_631 are separated by 63 nucleotides
Pf6N2E2_628 - 2-ketogluconate transporter, at 715,226 to 716,524
_628
Pf6N2E2_629 - 2-ketogluconate kinase (EC 2.7.1.13), at 716,617 to 717,591
_629
Pf6N2E2_630 - Epimerase KguE, at 717,584 to 718,366
_630
Pf6N2E2_631 - 2-ketogluconate utilization repressor PtxS, at 718,430 to 719,455
_631
Position (kb) 716
717
718 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 715.695 kb on + strand, within Pf6N2E2_628 at 715.854 kb on + strand, within Pf6N2E2_628 at 716.149 kb on - strand, within Pf6N2E2_628 at 716.149 kb on - strand, within Pf6N2E2_628 at 716.149 kb on - strand, within Pf6N2E2_628 at 716.310 kb on + strand, within Pf6N2E2_628 at 716.430 kb on + strand at 716.430 kb on + strand at 716.460 kb on + strand at 716.460 kb on + strand at 716.460 kb on + strand at 716.487 kb on + strand at 716.487 kb on + strand at 716.487 kb on + strand at 716.487 kb on + strand at 716.488 kb on - strand at 716.888 kb on + strand, within Pf6N2E2_629 at 716.889 kb on - strand, within Pf6N2E2_629 at 716.889 kb on - strand, within Pf6N2E2_629 at 717.272 kb on + strand, within Pf6N2E2_629 at 717.272 kb on + strand, within Pf6N2E2_629 at 717.272 kb on + strand, within Pf6N2E2_629 at 717.698 kb on + strand, within Pf6N2E2_630 at 717.699 kb on - strand, within Pf6N2E2_630 at 717.813 kb on + strand, within Pf6N2E2_630 at 717.813 kb on + strand, within Pf6N2E2_630 at 717.813 kb on + strand, within Pf6N2E2_630 at 717.814 kb on - strand, within Pf6N2E2_630 at 717.814 kb on - strand, within Pf6N2E2_630 at 718.071 kb on - strand, within Pf6N2E2_630 at 718.370 kb on + strand at 718.370 kb on + strand at 718.370 kb on + strand at 718.370 kb on + strand at 718.370 kb on + strand at 718.371 kb on - strand at 718.371 kb on - strand at 718.373 kb on + strand at 718.373 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 715,695 + Pf6N2E2_628 0.36 -0.3 715,854 + Pf6N2E2_628 0.48 -0.8 716,149 - Pf6N2E2_628 0.71 -0.1 716,149 - Pf6N2E2_628 0.71 +1.7 716,149 - Pf6N2E2_628 0.71 -1.5 716,310 + Pf6N2E2_628 0.83 -1.4 716,430 + -2.0 716,430 + -1.0 716,460 + +1.0 716,460 + +0.5 716,460 + +0.5 716,487 + +1.2 716,487 + +0.0 716,487 + -1.7 716,487 + +0.1 716,488 - -0.4 716,888 + Pf6N2E2_629 0.28 -1.9 716,889 - Pf6N2E2_629 0.28 -1.8 716,889 - Pf6N2E2_629 0.28 -0.6 717,272 + Pf6N2E2_629 0.67 -2.2 717,272 + Pf6N2E2_629 0.67 -0.6 717,272 + Pf6N2E2_629 0.67 -0.8 717,698 + Pf6N2E2_630 0.15 -0.7 717,699 - Pf6N2E2_630 0.15 -1.7 717,813 + Pf6N2E2_630 0.29 +1.0 717,813 + Pf6N2E2_630 0.29 +0.5 717,813 + Pf6N2E2_630 0.29 +0.3 717,814 - Pf6N2E2_630 0.29 +0.2 717,814 - Pf6N2E2_630 0.29 +1.8 718,071 - Pf6N2E2_630 0.62 +2.8 718,370 + -0.5 718,370 + +1.1 718,370 + -1.2 718,370 + -1.0 718,370 + -0.6 718,371 - -0.9 718,371 - +0.9 718,373 + +0.2 718,373 + +0.2
Or see this region's nucleotide sequence