Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5464 and Pf6N2E2_5465 are separated by 42 nucleotides Pf6N2E2_5465 and Pf6N2E2_5466 overlap by 4 nucleotides
Pf6N2E2_5463 - sensor histidine kinase, at 6,274,973 to 6,276,775
_5463
Pf6N2E2_5464 - Cytochrome c551 peroxidase (EC 1.11.1.5), at 6,276,772 to 6,277,731
_5464
Pf6N2E2_5465 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 6,277,774 to 6,279,648
_5465
Pf6N2E2_5466 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 6,279,645 to 6,281,936
_5466
Position (kb)
6277
6278
6279
6280 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 6276.776 kb on - strand at 6276.835 kb on - strand at 6276.866 kb on + strand at 6276.866 kb on + strand at 6276.866 kb on + strand at 6276.867 kb on - strand at 6276.867 kb on - strand at 6277.046 kb on + strand, within Pf6N2E2_5464 at 6277.046 kb on + strand, within Pf6N2E2_5464 at 6277.046 kb on + strand, within Pf6N2E2_5464 at 6277.047 kb on - strand, within Pf6N2E2_5464 at 6277.047 kb on - strand, within Pf6N2E2_5464 at 6277.121 kb on + strand, within Pf6N2E2_5464 at 6277.121 kb on + strand, within Pf6N2E2_5464 at 6277.122 kb on - strand, within Pf6N2E2_5464 at 6277.122 kb on - strand, within Pf6N2E2_5464 at 6277.187 kb on + strand, within Pf6N2E2_5464 at 6277.187 kb on + strand, within Pf6N2E2_5464 at 6277.229 kb on + strand, within Pf6N2E2_5464 at 6277.459 kb on - strand, within Pf6N2E2_5464 at 6277.723 kb on + strand at 6277.723 kb on + strand at 6277.723 kb on + strand at 6277.724 kb on - strand at 6277.724 kb on - strand at 6277.761 kb on - strand at 6277.853 kb on + strand at 6277.853 kb on + strand at 6277.853 kb on + strand at 6277.853 kb on + strand at 6277.853 kb on + strand at 6277.853 kb on + strand at 6277.853 kb on + strand at 6277.854 kb on - strand at 6277.854 kb on - strand at 6277.854 kb on - strand at 6277.854 kb on - strand at 6277.856 kb on + strand at 6277.857 kb on - strand at 6277.857 kb on - strand at 6277.857 kb on - strand at 6278.060 kb on + strand, within Pf6N2E2_5465 at 6278.060 kb on + strand, within Pf6N2E2_5465 at 6278.060 kb on + strand, within Pf6N2E2_5465 at 6278.060 kb on + strand, within Pf6N2E2_5465 at 6278.061 kb on - strand, within Pf6N2E2_5465 at 6278.114 kb on - strand, within Pf6N2E2_5465 at 6278.167 kb on + strand, within Pf6N2E2_5465 at 6278.167 kb on + strand, within Pf6N2E2_5465 at 6278.236 kb on - strand, within Pf6N2E2_5465 at 6278.291 kb on + strand, within Pf6N2E2_5465 at 6278.291 kb on + strand, within Pf6N2E2_5465 at 6278.292 kb on - strand, within Pf6N2E2_5465 at 6278.501 kb on + strand, within Pf6N2E2_5465 at 6278.937 kb on + strand, within Pf6N2E2_5465 at 6279.071 kb on + strand, within Pf6N2E2_5465 at 6279.088 kb on + strand, within Pf6N2E2_5465 at 6279.089 kb on - strand, within Pf6N2E2_5465 at 6279.089 kb on - strand, within Pf6N2E2_5465 at 6279.238 kb on + strand, within Pf6N2E2_5465 at 6279.238 kb on + strand, within Pf6N2E2_5465 at 6279.238 kb on + strand, within Pf6N2E2_5465 at 6279.405 kb on - strand, within Pf6N2E2_5465 at 6279.405 kb on - strand, within Pf6N2E2_5465 at 6279.664 kb on - strand at 6279.763 kb on + strand at 6279.763 kb on + strand at 6279.763 kb on + strand at 6279.763 kb on + strand at 6279.763 kb on + strand at 6279.764 kb on - strand at 6279.764 kb on - strand at 6279.764 kb on - strand at 6279.764 kb on - strand at 6279.764 kb on - strand at 6279.974 kb on - strand, within Pf6N2E2_5466 at 6279.974 kb on - strand, within Pf6N2E2_5466 at 6279.988 kb on + strand, within Pf6N2E2_5466 at 6279.988 kb on + strand, within Pf6N2E2_5466 at 6280.153 kb on + strand, within Pf6N2E2_5466 at 6280.153 kb on + strand, within Pf6N2E2_5466 at 6280.153 kb on + strand, within Pf6N2E2_5466 at 6280.154 kb on - strand, within Pf6N2E2_5466 at 6280.158 kb on + strand, within Pf6N2E2_5466 at 6280.158 kb on + strand, within Pf6N2E2_5466 at 6280.158 kb on + strand, within Pf6N2E2_5466 at 6280.417 kb on + strand, within Pf6N2E2_5466 at 6280.418 kb on - strand, within Pf6N2E2_5466 at 6280.418 kb on - strand, within Pf6N2E2_5466 at 6280.497 kb on - strand, within Pf6N2E2_5466 at 6280.519 kb on + strand, within Pf6N2E2_5466 at 6280.607 kb on - strand, within Pf6N2E2_5466
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 6,276,776 - +0.3 6,276,835 - -1.0 6,276,866 + +1.4 6,276,866 + +1.3 6,276,866 + +0.6 6,276,867 - +0.1 6,276,867 - -1.1 6,277,046 + Pf6N2E2_5464 0.29 +0.4 6,277,046 + Pf6N2E2_5464 0.29 -0.4 6,277,046 + Pf6N2E2_5464 0.29 +2.0 6,277,047 - Pf6N2E2_5464 0.29 +0.5 6,277,047 - Pf6N2E2_5464 0.29 -0.7 6,277,121 + Pf6N2E2_5464 0.36 -2.2 6,277,121 + Pf6N2E2_5464 0.36 +1.3 6,277,122 - Pf6N2E2_5464 0.36 +0.8 6,277,122 - Pf6N2E2_5464 0.36 -0.7 6,277,187 + Pf6N2E2_5464 0.43 -1.0 6,277,187 + Pf6N2E2_5464 0.43 -1.4 6,277,229 + Pf6N2E2_5464 0.48 -1.6 6,277,459 - Pf6N2E2_5464 0.72 +1.9 6,277,723 + +0.1 6,277,723 + +0.3 6,277,723 + -0.2 6,277,724 - +0.2 6,277,724 - +0.0 6,277,761 - -0.6 6,277,853 + -1.9 6,277,853 + +1.4 6,277,853 + +5.4 6,277,853 + -2.8 6,277,853 + -0.4 6,277,853 + -1.1 6,277,853 + +1.0 6,277,854 - +0.5 6,277,854 - +0.6 6,277,854 - -1.5 6,277,854 - -0.3 6,277,856 + +0.8 6,277,857 - -2.7 6,277,857 - -1.6 6,277,857 - -1.3 6,278,060 + Pf6N2E2_5465 0.15 -1.6 6,278,060 + Pf6N2E2_5465 0.15 +1.0 6,278,060 + Pf6N2E2_5465 0.15 -0.6 6,278,060 + Pf6N2E2_5465 0.15 -1.6 6,278,061 - Pf6N2E2_5465 0.15 +0.7 6,278,114 - Pf6N2E2_5465 0.18 -1.7 6,278,167 + Pf6N2E2_5465 0.21 -0.2 6,278,167 + Pf6N2E2_5465 0.21 +0.2 6,278,236 - Pf6N2E2_5465 0.25 +0.3 6,278,291 + Pf6N2E2_5465 0.28 -0.4 6,278,291 + Pf6N2E2_5465 0.28 +0.1 6,278,292 - Pf6N2E2_5465 0.28 -1.3 6,278,501 + Pf6N2E2_5465 0.39 -1.2 6,278,937 + Pf6N2E2_5465 0.62 +0.5 6,279,071 + Pf6N2E2_5465 0.69 +0.0 6,279,088 + Pf6N2E2_5465 0.70 -1.2 6,279,089 - Pf6N2E2_5465 0.70 +1.0 6,279,089 - Pf6N2E2_5465 0.70 -0.3 6,279,238 + Pf6N2E2_5465 0.78 -1.4 6,279,238 + Pf6N2E2_5465 0.78 +0.0 6,279,238 + Pf6N2E2_5465 0.78 -2.0 6,279,405 - Pf6N2E2_5465 0.87 -0.9 6,279,405 - Pf6N2E2_5465 0.87 -1.7 6,279,664 - -0.4 6,279,763 + -0.7 6,279,763 + +0.6 6,279,763 + -1.2 6,279,763 + +0.4 6,279,763 + +1.3 6,279,764 - +1.3 6,279,764 - -0.7 6,279,764 - -0.5 6,279,764 - -0.8 6,279,764 - +0.1 6,279,974 - Pf6N2E2_5466 0.14 -1.6 6,279,974 - Pf6N2E2_5466 0.14 -0.5 6,279,988 + Pf6N2E2_5466 0.15 -1.5 6,279,988 + Pf6N2E2_5466 0.15 -0.5 6,280,153 + Pf6N2E2_5466 0.22 +1.1 6,280,153 + Pf6N2E2_5466 0.22 +2.0 6,280,153 + Pf6N2E2_5466 0.22 -1.3 6,280,154 - Pf6N2E2_5466 0.22 -1.7 6,280,158 + Pf6N2E2_5466 0.22 -0.3 6,280,158 + Pf6N2E2_5466 0.22 -2.4 6,280,158 + Pf6N2E2_5466 0.22 +1.8 6,280,417 + Pf6N2E2_5466 0.34 +1.2 6,280,418 - Pf6N2E2_5466 0.34 -1.7 6,280,418 - Pf6N2E2_5466 0.34 +1.1 6,280,497 - Pf6N2E2_5466 0.37 -1.4 6,280,519 + Pf6N2E2_5466 0.38 -1.1 6,280,607 - Pf6N2E2_5466 0.42 -1.0
Or see this region's nucleotide sequence