Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_488 and Pf6N2E2_489 are separated by 42 nucleotides Pf6N2E2_489 and Pf6N2E2_490 are separated by 173 nucleotides
Pf6N2E2_488 - Gluconolactonase (EC 3.1.1.17), at 554,449 to 555,354
_488
Pf6N2E2_489 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 555,397 to 556,218
_489
Pf6N2E2_490 - PhaK-like protein, at 556,392 to 557,693
_490
Position (kb)
555
556
557 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 554.711 kb on + strand, within Pf6N2E2_488 at 554.712 kb on - strand, within Pf6N2E2_488 at 554.814 kb on - strand, within Pf6N2E2_488 at 554.861 kb on + strand, within Pf6N2E2_488 at 554.861 kb on + strand, within Pf6N2E2_488 at 554.861 kb on + strand, within Pf6N2E2_488 at 554.861 kb on + strand, within Pf6N2E2_488 at 554.861 kb on + strand, within Pf6N2E2_488 at 554.861 kb on + strand, within Pf6N2E2_488 at 554.862 kb on - strand, within Pf6N2E2_488 at 554.960 kb on + strand, within Pf6N2E2_488 at 554.961 kb on - strand, within Pf6N2E2_488 at 554.961 kb on - strand, within Pf6N2E2_488 at 554.961 kb on - strand, within Pf6N2E2_488 at 555.231 kb on + strand, within Pf6N2E2_488 at 555.231 kb on + strand, within Pf6N2E2_488 at 555.276 kb on - strand at 555.440 kb on + strand at 555.584 kb on + strand, within Pf6N2E2_489 at 555.584 kb on + strand, within Pf6N2E2_489 at 555.615 kb on - strand, within Pf6N2E2_489 at 555.747 kb on - strand, within Pf6N2E2_489 at 555.770 kb on + strand, within Pf6N2E2_489 at 555.770 kb on + strand, within Pf6N2E2_489 at 555.771 kb on - strand, within Pf6N2E2_489 at 555.771 kb on - strand, within Pf6N2E2_489 at 555.791 kb on + strand, within Pf6N2E2_489 at 555.791 kb on + strand, within Pf6N2E2_489 at 555.791 kb on + strand, within Pf6N2E2_489 at 555.843 kb on - strand, within Pf6N2E2_489 at 555.911 kb on + strand, within Pf6N2E2_489 at 555.911 kb on + strand, within Pf6N2E2_489 at 555.911 kb on + strand, within Pf6N2E2_489 at 555.911 kb on + strand, within Pf6N2E2_489 at 556.177 kb on + strand at 556.177 kb on + strand at 556.177 kb on + strand at 556.178 kb on - strand at 556.178 kb on - strand at 556.178 kb on - strand at 556.224 kb on + strand at 556.225 kb on - strand at 556.237 kb on + strand at 556.237 kb on + strand at 556.246 kb on + strand at 556.246 kb on + strand at 556.300 kb on + strand at 556.393 kb on + strand at 556.453 kb on + strand at 556.453 kb on + strand at 556.516 kb on - strand at 557.014 kb on + strand, within Pf6N2E2_490 at 557.014 kb on + strand, within Pf6N2E2_490
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 554,711 + Pf6N2E2_488 0.29 -2.0 554,712 - Pf6N2E2_488 0.29 -1.2 554,814 - Pf6N2E2_488 0.40 +0.8 554,861 + Pf6N2E2_488 0.45 +0.0 554,861 + Pf6N2E2_488 0.45 -1.0 554,861 + Pf6N2E2_488 0.45 +1.4 554,861 + Pf6N2E2_488 0.45 -0.6 554,861 + Pf6N2E2_488 0.45 +0.1 554,861 + Pf6N2E2_488 0.45 -0.9 554,862 - Pf6N2E2_488 0.46 +0.4 554,960 + Pf6N2E2_488 0.56 -0.4 554,961 - Pf6N2E2_488 0.57 +0.2 554,961 - Pf6N2E2_488 0.57 -0.5 554,961 - Pf6N2E2_488 0.57 -0.3 555,231 + Pf6N2E2_488 0.86 +2.6 555,231 + Pf6N2E2_488 0.86 +0.8 555,276 - -0.2 555,440 + +1.5 555,584 + Pf6N2E2_489 0.23 +0.3 555,584 + Pf6N2E2_489 0.23 +1.4 555,615 - Pf6N2E2_489 0.27 +0.3 555,747 - Pf6N2E2_489 0.43 -0.6 555,770 + Pf6N2E2_489 0.45 -2.3 555,770 + Pf6N2E2_489 0.45 -0.9 555,771 - Pf6N2E2_489 0.45 +0.7 555,771 - Pf6N2E2_489 0.45 +0.4 555,791 + Pf6N2E2_489 0.48 -1.7 555,791 + Pf6N2E2_489 0.48 -1.9 555,791 + Pf6N2E2_489 0.48 -1.8 555,843 - Pf6N2E2_489 0.54 +1.2 555,911 + Pf6N2E2_489 0.63 +2.7 555,911 + Pf6N2E2_489 0.63 +2.5 555,911 + Pf6N2E2_489 0.63 +0.9 555,911 + Pf6N2E2_489 0.63 +0.4 556,177 + +1.2 556,177 + -0.8 556,177 + -0.5 556,178 - +0.6 556,178 - +0.6 556,178 - -0.7 556,224 + -0.1 556,225 - -1.0 556,237 + -0.4 556,237 + +1.3 556,246 + +0.0 556,246 + +1.0 556,300 + -0.3 556,393 + -0.9 556,453 + -0.2 556,453 + -0.1 556,516 - +0.7 557,014 + Pf6N2E2_490 0.48 -0.4 557,014 + Pf6N2E2_490 0.48 +0.2
Or see this region's nucleotide sequence