Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4548

Experiment: L-Serine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4547 and Pf6N2E2_4548 overlap by 23 nucleotidesPf6N2E2_4548 and Pf6N2E2_4549 overlap by 4 nucleotidesPf6N2E2_4549 and Pf6N2E2_4550 are separated by 14 nucleotides Pf6N2E2_4547 - Type III secretion cytoplasmic protein (YscL), at 5,278,586 to 5,279,179 _4547 Pf6N2E2_4548 - hypothetical protein, at 5,279,157 to 5,279,747 _4548 Pf6N2E2_4549 - Type III secretion bridge between inner and outermembrane lipoprotein (YscJ,HrcJ,EscJ, PscJ), at 5,279,744 to 5,280,631 _4549 Pf6N2E2_4550 - hypothetical protein, at 5,280,646 to 5,281,020 _4550 Position (kb) 5279 5280Strain fitness (log2 ratio) -2 -1 0 1 2at 5278.215 kb on - strandat 5278.215 kb on - strandat 5278.215 kb on - strandat 5278.215 kb on - strandat 5278.215 kb on - strandat 5278.215 kb on - strandat 5278.219 kb on - strandat 5278.219 kb on - strandat 5278.244 kb on + strandat 5278.244 kb on + strandat 5278.381 kb on - strandat 5278.381 kb on - strandat 5278.443 kb on + strandat 5278.445 kb on + strandat 5278.445 kb on + strandat 5278.493 kb on + strandat 5278.493 kb on + strandat 5278.493 kb on + strandat 5278.493 kb on + strandat 5278.493 kb on + strandat 5278.493 kb on + strandat 5278.494 kb on - strandat 5278.530 kb on + strandat 5278.531 kb on - strandat 5278.531 kb on - strandat 5278.531 kb on - strandat 5278.622 kb on + strandat 5278.656 kb on + strand, within Pf6N2E2_4547at 5278.656 kb on + strand, within Pf6N2E2_4547at 5278.656 kb on + strand, within Pf6N2E2_4547at 5278.656 kb on + strand, within Pf6N2E2_4547at 5278.656 kb on + strand, within Pf6N2E2_4547at 5278.656 kb on + strand, within Pf6N2E2_4547at 5278.656 kb on + strand, within Pf6N2E2_4547at 5278.657 kb on - strand, within Pf6N2E2_4547at 5278.657 kb on - strand, within Pf6N2E2_4547at 5278.657 kb on - strand, within Pf6N2E2_4547at 5278.657 kb on - strand, within Pf6N2E2_4547at 5278.660 kb on + strand, within Pf6N2E2_4547at 5278.660 kb on + strand, within Pf6N2E2_4547at 5278.660 kb on + strand, within Pf6N2E2_4547at 5278.660 kb on + strand, within Pf6N2E2_4547at 5278.660 kb on + strand, within Pf6N2E2_4547at 5278.661 kb on - strand, within Pf6N2E2_4547at 5278.661 kb on - strand, within Pf6N2E2_4547at 5279.121 kb on + strandat 5279.198 kb on + strandat 5279.198 kb on + strandat 5279.199 kb on - strandat 5279.199 kb on - strandat 5279.199 kb on - strandat 5279.255 kb on + strand, within Pf6N2E2_4548at 5279.513 kb on - strand, within Pf6N2E2_4548at 5279.680 kb on + strand, within Pf6N2E2_4548at 5279.916 kb on + strand, within Pf6N2E2_4549at 5279.916 kb on + strand, within Pf6N2E2_4549at 5279.916 kb on + strand, within Pf6N2E2_4549at 5279.916 kb on + strand, within Pf6N2E2_4549at 5279.917 kb on - strand, within Pf6N2E2_4549at 5279.917 kb on - strand, within Pf6N2E2_4549at 5280.018 kb on + strand, within Pf6N2E2_4549at 5280.018 kb on + strand, within Pf6N2E2_4549at 5280.018 kb on + strand, within Pf6N2E2_4549at 5280.019 kb on - strand, within Pf6N2E2_4549at 5280.019 kb on - strand, within Pf6N2E2_4549at 5280.063 kb on - strand, within Pf6N2E2_4549at 5280.063 kb on - strand, within Pf6N2E2_4549at 5280.071 kb on + strand, within Pf6N2E2_4549at 5280.172 kb on + strand, within Pf6N2E2_4549at 5280.172 kb on + strand, within Pf6N2E2_4549at 5280.173 kb on - strand, within Pf6N2E2_4549at 5280.181 kb on + strand, within Pf6N2E2_4549at 5280.272 kb on + strand, within Pf6N2E2_4549at 5280.312 kb on + strand, within Pf6N2E2_4549at 5280.312 kb on + strand, within Pf6N2E2_4549at 5280.312 kb on + strand, within Pf6N2E2_4549at 5280.312 kb on + strand, within Pf6N2E2_4549at 5280.312 kb on + strand, within Pf6N2E2_4549at 5280.312 kb on + strand, within Pf6N2E2_4549at 5280.312 kb on + strand, within Pf6N2E2_4549at 5280.312 kb on + strand, within Pf6N2E2_4549at 5280.313 kb on - strand, within Pf6N2E2_4549at 5280.313 kb on - strand, within Pf6N2E2_4549at 5280.313 kb on - strand, within Pf6N2E2_4549at 5280.313 kb on - strand, within Pf6N2E2_4549at 5280.319 kb on - strand, within Pf6N2E2_4549at 5280.648 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Serine (N)
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5,278,215 - -0.8
5,278,215 - +1.3
5,278,215 - +2.5
5,278,215 - -2.4
5,278,215 - +1.2
5,278,215 - +1.1
5,278,219 - +0.5
5,278,219 - +0.2
5,278,244 + -0.7
5,278,244 + +0.4
5,278,381 - -0.1
5,278,381 - +1.4
5,278,443 + +0.5
5,278,445 + -1.5
5,278,445 + -0.2
5,278,493 + -1.3
5,278,493 + -1.3
5,278,493 + +2.5
5,278,493 + -0.6
5,278,493 + -1.5
5,278,493 + +0.3
5,278,494 - +1.5
5,278,530 + -0.8
5,278,531 - -1.4
5,278,531 - -1.9
5,278,531 - +0.8
5,278,622 + +2.2
5,278,656 + Pf6N2E2_4547 0.12 +0.1
5,278,656 + Pf6N2E2_4547 0.12 +2.2
5,278,656 + Pf6N2E2_4547 0.12 -1.9
5,278,656 + Pf6N2E2_4547 0.12 -0.9
5,278,656 + Pf6N2E2_4547 0.12 -0.5
5,278,656 + Pf6N2E2_4547 0.12 +0.5
5,278,656 + Pf6N2E2_4547 0.12 +2.6
5,278,657 - Pf6N2E2_4547 0.12 -0.7
5,278,657 - Pf6N2E2_4547 0.12 +0.9
5,278,657 - Pf6N2E2_4547 0.12 +1.0
5,278,657 - Pf6N2E2_4547 0.12 -0.1
5,278,660 + Pf6N2E2_4547 0.12 -0.8
5,278,660 + Pf6N2E2_4547 0.12 -0.2
5,278,660 + Pf6N2E2_4547 0.12 +0.6
5,278,660 + Pf6N2E2_4547 0.12 +1.2
5,278,660 + Pf6N2E2_4547 0.12 +0.7
5,278,661 - Pf6N2E2_4547 0.13 -1.5
5,278,661 - Pf6N2E2_4547 0.13 -0.3
5,279,121 + +0.9
5,279,198 + +0.2
5,279,198 + +0.2
5,279,199 - +1.5
5,279,199 - +0.1
5,279,199 - +0.3
5,279,255 + Pf6N2E2_4548 0.17 -2.0
5,279,513 - Pf6N2E2_4548 0.60 +0.1
5,279,680 + Pf6N2E2_4548 0.88 -0.1
5,279,916 + Pf6N2E2_4549 0.19 +0.9
5,279,916 + Pf6N2E2_4549 0.19 +0.3
5,279,916 + Pf6N2E2_4549 0.19 -2.2
5,279,916 + Pf6N2E2_4549 0.19 -1.5
5,279,917 - Pf6N2E2_4549 0.19 +1.9
5,279,917 - Pf6N2E2_4549 0.19 -0.7
5,280,018 + Pf6N2E2_4549 0.31 +0.0
5,280,018 + Pf6N2E2_4549 0.31 -1.3
5,280,018 + Pf6N2E2_4549 0.31 -0.9
5,280,019 - Pf6N2E2_4549 0.31 -0.2
5,280,019 - Pf6N2E2_4549 0.31 -1.5
5,280,063 - Pf6N2E2_4549 0.36 +0.7
5,280,063 - Pf6N2E2_4549 0.36 -0.2
5,280,071 + Pf6N2E2_4549 0.37 +1.6
5,280,172 + Pf6N2E2_4549 0.48 -0.1
5,280,172 + Pf6N2E2_4549 0.48 +1.5
5,280,173 - Pf6N2E2_4549 0.48 -0.4
5,280,181 + Pf6N2E2_4549 0.49 -1.0
5,280,272 + Pf6N2E2_4549 0.59 -0.6
5,280,312 + Pf6N2E2_4549 0.64 -1.4
5,280,312 + Pf6N2E2_4549 0.64 -0.4
5,280,312 + Pf6N2E2_4549 0.64 -1.8
5,280,312 + Pf6N2E2_4549 0.64 +0.9
5,280,312 + Pf6N2E2_4549 0.64 -0.1
5,280,312 + Pf6N2E2_4549 0.64 +2.5
5,280,312 + Pf6N2E2_4549 0.64 -0.1
5,280,312 + Pf6N2E2_4549 0.64 -0.9
5,280,313 - Pf6N2E2_4549 0.64 -0.5
5,280,313 - Pf6N2E2_4549 0.64 -0.6
5,280,313 - Pf6N2E2_4549 0.64 -0.5
5,280,313 - Pf6N2E2_4549 0.64 +1.1
5,280,319 - Pf6N2E2_4549 0.65 -0.1
5,280,648 - -1.9

Or see this region's nucleotide sequence