Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_389 and Pf6N2E2_390 are separated by 58 nucleotides Pf6N2E2_390 and Pf6N2E2_391 are separated by 48 nucleotides Pf6N2E2_391 and Pf6N2E2_392 are separated by 21 nucleotides
Pf6N2E2_389 - Choline dehydrogenase (EC 1.1.99.1), at 456,997 to 458,562
_389
Pf6N2E2_390 - hypothetical protein, at 458,621 to 459,394
_390
Pf6N2E2_391 - hypothetical protein, at 459,443 to 460,921
_391
Pf6N2E2_392 - Transcriptional regulator, at 460,943 to 461,851
_392
Position (kb)
459
460
461 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 458.538 kb on - strand at 458.538 kb on - strand at 458.794 kb on - strand, within Pf6N2E2_390 at 458.794 kb on - strand, within Pf6N2E2_390 at 458.794 kb on - strand, within Pf6N2E2_390 at 458.943 kb on + strand, within Pf6N2E2_390 at 458.944 kb on - strand, within Pf6N2E2_390 at 459.054 kb on + strand, within Pf6N2E2_390 at 459.054 kb on + strand, within Pf6N2E2_390 at 459.054 kb on + strand, within Pf6N2E2_390 at 459.055 kb on - strand, within Pf6N2E2_390 at 459.288 kb on - strand, within Pf6N2E2_390 at 459.288 kb on - strand, within Pf6N2E2_390 at 459.310 kb on + strand, within Pf6N2E2_390 at 459.310 kb on + strand, within Pf6N2E2_390 at 459.310 kb on + strand, within Pf6N2E2_390 at 459.311 kb on - strand, within Pf6N2E2_390 at 459.311 kb on - strand, within Pf6N2E2_390 at 459.311 kb on - strand, within Pf6N2E2_390 at 459.311 kb on - strand, within Pf6N2E2_390 at 459.311 kb on - strand, within Pf6N2E2_390 at 459.459 kb on + strand at 459.899 kb on + strand, within Pf6N2E2_391 at 459.899 kb on + strand, within Pf6N2E2_391 at 459.900 kb on - strand, within Pf6N2E2_391 at 459.900 kb on - strand, within Pf6N2E2_391 at 460.310 kb on + strand, within Pf6N2E2_391 at 460.310 kb on + strand, within Pf6N2E2_391 at 460.473 kb on - strand, within Pf6N2E2_391 at 460.668 kb on + strand, within Pf6N2E2_391 at 460.669 kb on - strand, within Pf6N2E2_391 at 460.783 kb on + strand at 460.848 kb on + strand at 460.848 kb on + strand at 460.848 kb on + strand at 460.849 kb on - strand at 460.849 kb on - strand at 460.849 kb on - strand at 461.022 kb on + strand at 461.023 kb on - strand at 461.025 kb on + strand at 461.026 kb on - strand at 461.026 kb on - strand at 461.026 kb on - strand at 461.308 kb on + strand, within Pf6N2E2_392 at 461.313 kb on + strand, within Pf6N2E2_392 at 461.313 kb on + strand, within Pf6N2E2_392 at 461.314 kb on - strand, within Pf6N2E2_392 at 461.314 kb on - strand, within Pf6N2E2_392 at 461.326 kb on - strand, within Pf6N2E2_392 at 461.326 kb on - strand, within Pf6N2E2_392 at 461.350 kb on - strand, within Pf6N2E2_392 at 461.383 kb on + strand, within Pf6N2E2_392 at 461.394 kb on - strand, within Pf6N2E2_392 at 461.477 kb on + strand, within Pf6N2E2_392 at 461.478 kb on - strand, within Pf6N2E2_392 at 461.677 kb on + strand, within Pf6N2E2_392 at 461.686 kb on + strand, within Pf6N2E2_392 at 461.834 kb on + strand at 461.842 kb on + strand at 461.842 kb on + strand at 461.916 kb on + strand at 461.917 kb on - strand at 461.917 kb on - strand at 461.917 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 458,538 - -0.2 458,538 - -1.5 458,794 - Pf6N2E2_390 0.22 -1.8 458,794 - Pf6N2E2_390 0.22 +2.9 458,794 - Pf6N2E2_390 0.22 -0.3 458,943 + Pf6N2E2_390 0.42 -1.1 458,944 - Pf6N2E2_390 0.42 -0.2 459,054 + Pf6N2E2_390 0.56 +1.7 459,054 + Pf6N2E2_390 0.56 +0.1 459,054 + Pf6N2E2_390 0.56 +0.5 459,055 - Pf6N2E2_390 0.56 +0.6 459,288 - Pf6N2E2_390 0.86 -1.2 459,288 - Pf6N2E2_390 0.86 -0.8 459,310 + Pf6N2E2_390 0.89 +1.1 459,310 + Pf6N2E2_390 0.89 +0.7 459,310 + Pf6N2E2_390 0.89 +0.2 459,311 - Pf6N2E2_390 0.89 +2.8 459,311 - Pf6N2E2_390 0.89 -1.4 459,311 - Pf6N2E2_390 0.89 -1.3 459,311 - Pf6N2E2_390 0.89 +0.6 459,311 - Pf6N2E2_390 0.89 +0.1 459,459 + -1.4 459,899 + Pf6N2E2_391 0.31 +0.7 459,899 + Pf6N2E2_391 0.31 +0.1 459,900 - Pf6N2E2_391 0.31 -0.2 459,900 - Pf6N2E2_391 0.31 -1.1 460,310 + Pf6N2E2_391 0.59 -0.9 460,310 + Pf6N2E2_391 0.59 -0.3 460,473 - Pf6N2E2_391 0.70 -0.3 460,668 + Pf6N2E2_391 0.83 +0.2 460,669 - Pf6N2E2_391 0.83 -0.6 460,783 + -0.3 460,848 + +0.5 460,848 + -2.3 460,848 + +1.3 460,849 - +0.3 460,849 - -1.1 460,849 - +0.2 461,022 + -0.1 461,023 - +0.6 461,025 + -0.6 461,026 - -0.8 461,026 - -1.6 461,026 - +1.9 461,308 + Pf6N2E2_392 0.40 +0.8 461,313 + Pf6N2E2_392 0.41 +1.6 461,313 + Pf6N2E2_392 0.41 +0.1 461,314 - Pf6N2E2_392 0.41 -0.9 461,314 - Pf6N2E2_392 0.41 +0.3 461,326 - Pf6N2E2_392 0.42 -2.3 461,326 - Pf6N2E2_392 0.42 +0.3 461,350 - Pf6N2E2_392 0.45 +0.7 461,383 + Pf6N2E2_392 0.48 +1.2 461,394 - Pf6N2E2_392 0.50 -0.3 461,477 + Pf6N2E2_392 0.59 -1.1 461,478 - Pf6N2E2_392 0.59 +0.2 461,677 + Pf6N2E2_392 0.81 -1.4 461,686 + Pf6N2E2_392 0.82 +0.7 461,834 + +0.5 461,842 + +1.1 461,842 + -0.8 461,916 + +0.9 461,917 - +1.1 461,917 - +0.5 461,917 - +1.1
Or see this region's nucleotide sequence