Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3891 and Pf6N2E2_3892 are separated by 297 nucleotides Pf6N2E2_3892 and Pf6N2E2_3893 are separated by 78 nucleotides Pf6N2E2_3893 and Pf6N2E2_3894 are separated by 45 nucleotides
Pf6N2E2_3891 - Putative formate dehydrogenase oxidoreductase protein, at 4,522,692 to 4,525,040
_3891
Pf6N2E2_3892 - FIG01270322: hypothetical protein, at 4,525,338 to 4,525,817
_3892
Pf6N2E2_3893 - putative 4-hydroxybenzoyl-CoA thioesterase, at 4,525,896 to 4,526,315
_3893
Pf6N2E2_3894 - Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 4,526,361 to 4,527,587
_3894
Position (kb)
4525
4526 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 4524.450 kb on + strand, within Pf6N2E2_3891 at 4524.451 kb on - strand, within Pf6N2E2_3891 at 4524.484 kb on + strand, within Pf6N2E2_3891 at 4524.484 kb on + strand, within Pf6N2E2_3891 at 4524.534 kb on + strand, within Pf6N2E2_3891 at 4524.693 kb on + strand, within Pf6N2E2_3891 at 4524.693 kb on + strand, within Pf6N2E2_3891 at 4524.694 kb on - strand, within Pf6N2E2_3891 at 4524.694 kb on - strand, within Pf6N2E2_3891 at 4524.694 kb on - strand, within Pf6N2E2_3891 at 4524.694 kb on - strand, within Pf6N2E2_3891 at 4524.694 kb on - strand, within Pf6N2E2_3891 at 4524.694 kb on - strand, within Pf6N2E2_3891 at 4524.694 kb on - strand, within Pf6N2E2_3891 at 4524.694 kb on - strand, within Pf6N2E2_3891 at 4524.708 kb on + strand, within Pf6N2E2_3891 at 4524.708 kb on + strand, within Pf6N2E2_3891 at 4524.708 kb on + strand, within Pf6N2E2_3891 at 4524.708 kb on + strand, within Pf6N2E2_3891 at 4524.708 kb on + strand, within Pf6N2E2_3891 at 4524.709 kb on - strand, within Pf6N2E2_3891 at 4524.709 kb on - strand, within Pf6N2E2_3891 at 4524.906 kb on + strand at 4524.906 kb on + strand at 4524.906 kb on + strand at 4524.906 kb on + strand at 4524.907 kb on - strand at 4524.907 kb on - strand at 4524.907 kb on - strand at 4525.046 kb on + strand at 4525.046 kb on + strand at 4525.046 kb on + strand at 4525.046 kb on + strand at 4525.046 kb on + strand at 4525.046 kb on + strand at 4525.046 kb on + strand at 4525.047 kb on - strand at 4525.047 kb on - strand at 4525.133 kb on + strand at 4525.133 kb on + strand at 4525.134 kb on - strand at 4525.134 kb on - strand at 4525.190 kb on - strand at 4525.279 kb on + strand at 4525.284 kb on + strand at 4525.285 kb on - strand at 4525.403 kb on + strand, within Pf6N2E2_3892 at 4525.491 kb on - strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.680 kb on + strand, within Pf6N2E2_3892 at 4525.681 kb on - strand, within Pf6N2E2_3892 at 4525.681 kb on - strand, within Pf6N2E2_3892 at 4525.681 kb on - strand, within Pf6N2E2_3892 at 4525.681 kb on - strand, within Pf6N2E2_3892 at 4525.681 kb on - strand, within Pf6N2E2_3892 at 4525.681 kb on - strand, within Pf6N2E2_3892 at 4525.681 kb on - strand, within Pf6N2E2_3892 at 4525.681 kb on - strand, within Pf6N2E2_3892 at 4525.681 kb on - strand, within Pf6N2E2_3892 at 4525.681 kb on - strand, within Pf6N2E2_3892 at 4525.713 kb on + strand, within Pf6N2E2_3892 at 4525.714 kb on - strand, within Pf6N2E2_3892 at 4525.714 kb on - strand, within Pf6N2E2_3892 at 4525.740 kb on + strand, within Pf6N2E2_3892 at 4525.743 kb on + strand, within Pf6N2E2_3892 at 4525.743 kb on + strand, within Pf6N2E2_3892 at 4525.744 kb on - strand, within Pf6N2E2_3892 at 4525.803 kb on + strand at 4525.803 kb on + strand at 4525.807 kb on - strand at 4525.829 kb on + strand at 4525.926 kb on + strand at 4525.927 kb on - strand at 4525.974 kb on + strand, within Pf6N2E2_3893 at 4525.974 kb on + strand, within Pf6N2E2_3893 at 4525.975 kb on - strand, within Pf6N2E2_3893 at 4525.975 kb on - strand, within Pf6N2E2_3893 at 4525.975 kb on - strand, within Pf6N2E2_3893 at 4525.975 kb on - strand, within Pf6N2E2_3893 at 4526.039 kb on - strand, within Pf6N2E2_3893 at 4526.116 kb on + strand, within Pf6N2E2_3893 at 4526.116 kb on + strand, within Pf6N2E2_3893 at 4526.117 kb on - strand, within Pf6N2E2_3893 at 4526.117 kb on - strand, within Pf6N2E2_3893 at 4526.117 kb on - strand, within Pf6N2E2_3893 at 4526.117 kb on - strand, within Pf6N2E2_3893 at 4526.117 kb on - strand, within Pf6N2E2_3893 at 4526.117 kb on - strand, within Pf6N2E2_3893 at 4526.117 kb on - strand, within Pf6N2E2_3893 at 4526.117 kb on - strand, within Pf6N2E2_3893 at 4526.117 kb on - strand, within Pf6N2E2_3893 at 4526.191 kb on + strand, within Pf6N2E2_3893 at 4526.191 kb on + strand, within Pf6N2E2_3893 at 4526.191 kb on + strand, within Pf6N2E2_3893 at 4526.191 kb on + strand, within Pf6N2E2_3893 at 4526.191 kb on + strand, within Pf6N2E2_3893 at 4526.192 kb on - strand, within Pf6N2E2_3893 at 4526.192 kb on - strand, within Pf6N2E2_3893 at 4526.192 kb on - strand, within Pf6N2E2_3893 at 4526.192 kb on - strand, within Pf6N2E2_3893 at 4526.192 kb on - strand, within Pf6N2E2_3893 at 4526.192 kb on - strand, within Pf6N2E2_3893 at 4526.192 kb on - strand, within Pf6N2E2_3893 at 4526.195 kb on + strand, within Pf6N2E2_3893 at 4526.195 kb on + strand, within Pf6N2E2_3893 at 4526.196 kb on - strand, within Pf6N2E2_3893 at 4526.196 kb on - strand, within Pf6N2E2_3893 at 4526.196 kb on - strand, within Pf6N2E2_3893 at 4526.222 kb on - strand, within Pf6N2E2_3893 at 4526.222 kb on - strand, within Pf6N2E2_3893 at 4526.222 kb on - strand, within Pf6N2E2_3893 at 4526.224 kb on + strand, within Pf6N2E2_3893 at 4526.224 kb on + strand, within Pf6N2E2_3893 at 4526.224 kb on + strand, within Pf6N2E2_3893 at 4526.224 kb on + strand, within Pf6N2E2_3893 at 4526.224 kb on + strand, within Pf6N2E2_3893 at 4526.224 kb on + strand, within Pf6N2E2_3893 at 4526.224 kb on + strand, within Pf6N2E2_3893 at 4526.225 kb on - strand, within Pf6N2E2_3893 at 4526.225 kb on - strand, within Pf6N2E2_3893 at 4526.225 kb on - strand, within Pf6N2E2_3893 at 4526.225 kb on - strand, within Pf6N2E2_3893 at 4526.248 kb on + strand, within Pf6N2E2_3893 at 4526.248 kb on + strand, within Pf6N2E2_3893 at 4526.249 kb on - strand, within Pf6N2E2_3893 at 4526.277 kb on - strand at 4526.277 kb on - strand at 4526.670 kb on + strand, within Pf6N2E2_3894 at 4526.743 kb on + strand, within Pf6N2E2_3894 at 4526.744 kb on - strand, within Pf6N2E2_3894 at 4526.749 kb on + strand, within Pf6N2E2_3894 at 4526.749 kb on + strand, within Pf6N2E2_3894 at 4526.749 kb on + strand, within Pf6N2E2_3894 at 4526.749 kb on + strand, within Pf6N2E2_3894 at 4526.750 kb on - strand, within Pf6N2E2_3894 at 4526.750 kb on - strand, within Pf6N2E2_3894 at 4526.750 kb on - strand, within Pf6N2E2_3894 at 4526.750 kb on - strand, within Pf6N2E2_3894 at 4526.750 kb on - strand, within Pf6N2E2_3894 at 4526.750 kb on - strand, within Pf6N2E2_3894 at 4526.806 kb on - strand, within Pf6N2E2_3894
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 4,524,450 + Pf6N2E2_3891 0.75 +2.0 4,524,451 - Pf6N2E2_3891 0.75 -0.1 4,524,484 + Pf6N2E2_3891 0.76 -0.1 4,524,484 + Pf6N2E2_3891 0.76 +0.3 4,524,534 + Pf6N2E2_3891 0.78 -0.4 4,524,693 + Pf6N2E2_3891 0.85 -0.4 4,524,693 + Pf6N2E2_3891 0.85 +1.3 4,524,694 - Pf6N2E2_3891 0.85 -2.1 4,524,694 - Pf6N2E2_3891 0.85 -2.1 4,524,694 - Pf6N2E2_3891 0.85 +0.2 4,524,694 - Pf6N2E2_3891 0.85 +0.3 4,524,694 - Pf6N2E2_3891 0.85 -0.0 4,524,694 - Pf6N2E2_3891 0.85 -1.8 4,524,694 - Pf6N2E2_3891 0.85 -0.1 4,524,694 - Pf6N2E2_3891 0.85 -0.8 4,524,708 + Pf6N2E2_3891 0.86 -0.0 4,524,708 + Pf6N2E2_3891 0.86 -0.8 4,524,708 + Pf6N2E2_3891 0.86 +0.2 4,524,708 + Pf6N2E2_3891 0.86 -0.7 4,524,708 + Pf6N2E2_3891 0.86 -0.7 4,524,709 - Pf6N2E2_3891 0.86 -0.8 4,524,709 - Pf6N2E2_3891 0.86 +2.2 4,524,906 + -0.9 4,524,906 + -0.1 4,524,906 + -0.5 4,524,906 + -0.9 4,524,907 - -1.4 4,524,907 - -1.0 4,524,907 - +0.3 4,525,046 + -0.1 4,525,046 + +1.5 4,525,046 + -0.4 4,525,046 + -0.4 4,525,046 + +0.1 4,525,046 + -1.1 4,525,046 + +3.0 4,525,047 - +0.0 4,525,047 - -0.6 4,525,133 + -0.3 4,525,133 + -1.7 4,525,134 - +1.7 4,525,134 - -1.1 4,525,190 - +0.5 4,525,279 + +1.1 4,525,284 + +1.4 4,525,285 - +0.9 4,525,403 + Pf6N2E2_3892 0.14 -0.0 4,525,491 - Pf6N2E2_3892 0.32 -1.7 4,525,680 + Pf6N2E2_3892 0.71 +0.3 4,525,680 + Pf6N2E2_3892 0.71 -1.7 4,525,680 + Pf6N2E2_3892 0.71 +2.4 4,525,680 + Pf6N2E2_3892 0.71 -0.7 4,525,680 + Pf6N2E2_3892 0.71 +0.5 4,525,680 + Pf6N2E2_3892 0.71 -1.1 4,525,680 + Pf6N2E2_3892 0.71 +0.6 4,525,680 + Pf6N2E2_3892 0.71 -0.1 4,525,680 + Pf6N2E2_3892 0.71 +2.5 4,525,680 + Pf6N2E2_3892 0.71 -0.1 4,525,680 + Pf6N2E2_3892 0.71 -0.2 4,525,680 + Pf6N2E2_3892 0.71 -1.1 4,525,680 + Pf6N2E2_3892 0.71 -0.0 4,525,680 + Pf6N2E2_3892 0.71 +1.4 4,525,680 + Pf6N2E2_3892 0.71 +0.3 4,525,680 + Pf6N2E2_3892 0.71 -1.9 4,525,680 + Pf6N2E2_3892 0.71 +0.4 4,525,680 + Pf6N2E2_3892 0.71 -2.6 4,525,681 - Pf6N2E2_3892 0.71 +2.7 4,525,681 - Pf6N2E2_3892 0.71 +1.6 4,525,681 - Pf6N2E2_3892 0.71 +1.9 4,525,681 - Pf6N2E2_3892 0.71 -0.9 4,525,681 - Pf6N2E2_3892 0.71 +0.3 4,525,681 - Pf6N2E2_3892 0.71 -0.3 4,525,681 - Pf6N2E2_3892 0.71 -0.7 4,525,681 - Pf6N2E2_3892 0.71 -2.1 4,525,681 - Pf6N2E2_3892 0.71 +0.4 4,525,681 - Pf6N2E2_3892 0.71 +2.4 4,525,713 + Pf6N2E2_3892 0.78 -2.0 4,525,714 - Pf6N2E2_3892 0.78 -0.2 4,525,714 - Pf6N2E2_3892 0.78 -0.6 4,525,740 + Pf6N2E2_3892 0.84 +0.5 4,525,743 + Pf6N2E2_3892 0.84 +1.9 4,525,743 + Pf6N2E2_3892 0.84 +1.8 4,525,744 - Pf6N2E2_3892 0.85 +1.8 4,525,803 + +3.5 4,525,803 + +2.3 4,525,807 - -1.0 4,525,829 + +1.4 4,525,926 + -2.4 4,525,927 - +0.9 4,525,974 + Pf6N2E2_3893 0.19 -0.6 4,525,974 + Pf6N2E2_3893 0.19 +0.9 4,525,975 - Pf6N2E2_3893 0.19 +0.5 4,525,975 - Pf6N2E2_3893 0.19 -0.8 4,525,975 - Pf6N2E2_3893 0.19 -0.0 4,525,975 - Pf6N2E2_3893 0.19 +0.6 4,526,039 - Pf6N2E2_3893 0.34 -0.8 4,526,116 + Pf6N2E2_3893 0.52 +1.6 4,526,116 + Pf6N2E2_3893 0.52 -2.6 4,526,117 - Pf6N2E2_3893 0.53 -0.0 4,526,117 - Pf6N2E2_3893 0.53 +0.6 4,526,117 - Pf6N2E2_3893 0.53 +0.8 4,526,117 - Pf6N2E2_3893 0.53 +0.3 4,526,117 - Pf6N2E2_3893 0.53 -0.4 4,526,117 - Pf6N2E2_3893 0.53 +0.4 4,526,117 - Pf6N2E2_3893 0.53 +1.4 4,526,117 - Pf6N2E2_3893 0.53 +0.4 4,526,117 - Pf6N2E2_3893 0.53 +0.1 4,526,191 + Pf6N2E2_3893 0.70 +0.7 4,526,191 + Pf6N2E2_3893 0.70 +1.0 4,526,191 + Pf6N2E2_3893 0.70 +1.5 4,526,191 + Pf6N2E2_3893 0.70 -1.1 4,526,191 + Pf6N2E2_3893 0.70 -1.4 4,526,192 - Pf6N2E2_3893 0.70 -1.4 4,526,192 - Pf6N2E2_3893 0.70 +1.1 4,526,192 - Pf6N2E2_3893 0.70 -0.1 4,526,192 - Pf6N2E2_3893 0.70 -0.5 4,526,192 - Pf6N2E2_3893 0.70 -0.5 4,526,192 - Pf6N2E2_3893 0.70 +0.4 4,526,192 - Pf6N2E2_3893 0.70 -0.1 4,526,195 + Pf6N2E2_3893 0.71 -0.1 4,526,195 + Pf6N2E2_3893 0.71 +0.6 4,526,196 - Pf6N2E2_3893 0.71 +0.5 4,526,196 - Pf6N2E2_3893 0.71 +2.0 4,526,196 - Pf6N2E2_3893 0.71 -0.1 4,526,222 - Pf6N2E2_3893 0.78 +1.1 4,526,222 - Pf6N2E2_3893 0.78 -0.4 4,526,222 - Pf6N2E2_3893 0.78 -1.1 4,526,224 + Pf6N2E2_3893 0.78 +4.0 4,526,224 + Pf6N2E2_3893 0.78 -1.7 4,526,224 + Pf6N2E2_3893 0.78 -1.0 4,526,224 + Pf6N2E2_3893 0.78 -0.3 4,526,224 + Pf6N2E2_3893 0.78 +0.4 4,526,224 + Pf6N2E2_3893 0.78 +0.2 4,526,224 + Pf6N2E2_3893 0.78 -2.3 4,526,225 - Pf6N2E2_3893 0.78 +0.9 4,526,225 - Pf6N2E2_3893 0.78 +0.5 4,526,225 - Pf6N2E2_3893 0.78 -0.2 4,526,225 - Pf6N2E2_3893 0.78 +0.6 4,526,248 + Pf6N2E2_3893 0.84 -0.1 4,526,248 + Pf6N2E2_3893 0.84 -2.1 4,526,249 - Pf6N2E2_3893 0.84 -1.4 4,526,277 - -0.3 4,526,277 - -0.4 4,526,670 + Pf6N2E2_3894 0.25 +0.3 4,526,743 + Pf6N2E2_3894 0.31 -1.1 4,526,744 - Pf6N2E2_3894 0.31 -0.9 4,526,749 + Pf6N2E2_3894 0.32 -1.0 4,526,749 + Pf6N2E2_3894 0.32 +0.3 4,526,749 + Pf6N2E2_3894 0.32 +1.0 4,526,749 + Pf6N2E2_3894 0.32 -2.2 4,526,750 - Pf6N2E2_3894 0.32 +0.5 4,526,750 - Pf6N2E2_3894 0.32 -1.1 4,526,750 - Pf6N2E2_3894 0.32 -0.1 4,526,750 - Pf6N2E2_3894 0.32 -1.4 4,526,750 - Pf6N2E2_3894 0.32 +1.0 4,526,750 - Pf6N2E2_3894 0.32 +0.4 4,526,806 - Pf6N2E2_3894 0.36 +2.6
Or see this region's nucleotide sequence