Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3013 and Pf6N2E2_3014 are separated by 9 nucleotides Pf6N2E2_3014 and Pf6N2E2_3015 overlap by 8 nucleotides Pf6N2E2_3015 and Pf6N2E2_3016 overlap by 1 nucleotides Pf6N2E2_3016 and Pf6N2E2_3017 are separated by 6 nucleotides Pf6N2E2_3017 and Pf6N2E2_3018 overlap by 4 nucleotides Pf6N2E2_3018 and Pf6N2E2_3019 are separated by 152 nucleotides
Pf6N2E2_3013 - FIG032563: Phage tail protein D, at 3,501,110 to 3,502,126
_3013
Pf6N2E2_3014 - P2-like prophage tail protein X, at 3,502,136 to 3,502,348
_3014
Pf6N2E2_3015 - hypothetical protein, at 3,502,341 to 3,502,724
_3015
Pf6N2E2_3016 - Phage tail length tape-measure protein, at 3,502,724 to 3,504,901
_3016
Pf6N2E2_3017 - hypothetical protein, at 3,504,908 to 3,505,033
_3017
Pf6N2E2_3018 - FIG00955390: hypothetical protein, at 3,505,030 to 3,505,596
_3018
Pf6N2E2_3019 - FIG00954559: hypothetical protein, at 3,505,749 to 3,506,255
_3019
Position (kb)
3502
3503
3504
3505 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3502.217 kb on + strand, within Pf6N2E2_3014 at 3502.217 kb on + strand, within Pf6N2E2_3014 at 3502.299 kb on + strand, within Pf6N2E2_3014 at 3502.543 kb on + strand, within Pf6N2E2_3015 at 3502.544 kb on - strand, within Pf6N2E2_3015 at 3502.544 kb on - strand, within Pf6N2E2_3015 at 3502.544 kb on - strand, within Pf6N2E2_3015 at 3502.729 kb on + strand at 3502.729 kb on + strand at 3502.730 kb on - strand at 3502.730 kb on - strand at 3502.866 kb on + strand at 3502.866 kb on + strand at 3502.867 kb on - strand at 3503.168 kb on + strand, within Pf6N2E2_3016 at 3503.207 kb on + strand, within Pf6N2E2_3016 at 3503.297 kb on - strand, within Pf6N2E2_3016 at 3503.297 kb on - strand, within Pf6N2E2_3016 at 3503.297 kb on - strand, within Pf6N2E2_3016 at 3503.306 kb on + strand, within Pf6N2E2_3016 at 3503.477 kb on + strand, within Pf6N2E2_3016 at 3503.548 kb on + strand, within Pf6N2E2_3016 at 3504.033 kb on - strand, within Pf6N2E2_3016 at 3504.608 kb on + strand, within Pf6N2E2_3016 at 3504.608 kb on + strand, within Pf6N2E2_3016 at 3504.609 kb on - strand, within Pf6N2E2_3016 at 3504.609 kb on - strand, within Pf6N2E2_3016 at 3504.609 kb on - strand, within Pf6N2E2_3016 at 3504.609 kb on - strand, within Pf6N2E2_3016 at 3504.626 kb on - strand, within Pf6N2E2_3016 at 3504.827 kb on - strand at 3504.835 kb on + strand at 3504.836 kb on - strand at 3504.836 kb on - strand at 3504.874 kb on - strand at 3504.874 kb on - strand at 3504.885 kb on + strand at 3504.886 kb on - strand at 3504.890 kb on - strand at 3504.890 kb on - strand at 3504.890 kb on - strand at 3504.909 kb on + strand at 3504.909 kb on + strand at 3504.910 kb on - strand at 3504.998 kb on + strand, within Pf6N2E2_3017 at 3504.999 kb on - strand, within Pf6N2E2_3017 at 3504.999 kb on - strand, within Pf6N2E2_3017 at 3505.006 kb on - strand, within Pf6N2E2_3017 at 3505.073 kb on - strand at 3505.291 kb on + strand, within Pf6N2E2_3018 at 3505.291 kb on + strand, within Pf6N2E2_3018 at 3505.292 kb on - strand, within Pf6N2E2_3018 at 3505.292 kb on - strand, within Pf6N2E2_3018 at 3505.361 kb on + strand, within Pf6N2E2_3018 at 3505.361 kb on + strand, within Pf6N2E2_3018 at 3505.361 kb on + strand, within Pf6N2E2_3018 at 3505.362 kb on - strand, within Pf6N2E2_3018 at 3505.362 kb on - strand, within Pf6N2E2_3018 at 3505.380 kb on - strand, within Pf6N2E2_3018 at 3505.502 kb on + strand, within Pf6N2E2_3018 at 3505.613 kb on + strand at 3505.614 kb on - strand at 3505.614 kb on - strand at 3505.864 kb on + strand, within Pf6N2E2_3019 at 3505.864 kb on + strand, within Pf6N2E2_3019 at 3505.865 kb on - strand, within Pf6N2E2_3019 at 3505.865 kb on - strand, within Pf6N2E2_3019 at 3505.865 kb on - strand, within Pf6N2E2_3019
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 3,502,217 + Pf6N2E2_3014 0.38 -1.3 3,502,217 + Pf6N2E2_3014 0.38 +1.2 3,502,299 + Pf6N2E2_3014 0.77 +0.2 3,502,543 + Pf6N2E2_3015 0.53 -0.4 3,502,544 - Pf6N2E2_3015 0.53 -1.5 3,502,544 - Pf6N2E2_3015 0.53 -0.4 3,502,544 - Pf6N2E2_3015 0.53 +1.0 3,502,729 + -0.4 3,502,729 + +0.6 3,502,730 - -0.1 3,502,730 - +0.4 3,502,866 + +1.1 3,502,866 + +1.2 3,502,867 - +0.9 3,503,168 + Pf6N2E2_3016 0.20 -0.2 3,503,207 + Pf6N2E2_3016 0.22 +2.0 3,503,297 - Pf6N2E2_3016 0.26 -0.0 3,503,297 - Pf6N2E2_3016 0.26 +1.8 3,503,297 - Pf6N2E2_3016 0.26 -1.1 3,503,306 + Pf6N2E2_3016 0.27 +0.3 3,503,477 + Pf6N2E2_3016 0.35 -0.4 3,503,548 + Pf6N2E2_3016 0.38 +0.4 3,504,033 - Pf6N2E2_3016 0.60 +0.9 3,504,608 + Pf6N2E2_3016 0.87 -0.7 3,504,608 + Pf6N2E2_3016 0.87 +1.1 3,504,609 - Pf6N2E2_3016 0.87 +1.5 3,504,609 - Pf6N2E2_3016 0.87 +1.6 3,504,609 - Pf6N2E2_3016 0.87 +1.0 3,504,609 - Pf6N2E2_3016 0.87 -2.3 3,504,626 - Pf6N2E2_3016 0.87 +1.2 3,504,827 - -1.6 3,504,835 + -0.9 3,504,836 - -1.1 3,504,836 - -2.6 3,504,874 - -2.2 3,504,874 - +0.7 3,504,885 + +0.5 3,504,886 - -0.6 3,504,890 - +0.0 3,504,890 - -0.7 3,504,890 - +1.4 3,504,909 + +0.7 3,504,909 + +1.3 3,504,910 - +0.0 3,504,998 + Pf6N2E2_3017 0.71 +0.6 3,504,999 - Pf6N2E2_3017 0.72 -1.0 3,504,999 - Pf6N2E2_3017 0.72 -0.5 3,505,006 - Pf6N2E2_3017 0.78 +1.2 3,505,073 - -0.3 3,505,291 + Pf6N2E2_3018 0.46 -0.2 3,505,291 + Pf6N2E2_3018 0.46 +1.1 3,505,292 - Pf6N2E2_3018 0.46 -1.4 3,505,292 - Pf6N2E2_3018 0.46 +1.3 3,505,361 + Pf6N2E2_3018 0.58 +0.8 3,505,361 + Pf6N2E2_3018 0.58 +0.5 3,505,361 + Pf6N2E2_3018 0.58 +0.5 3,505,362 - Pf6N2E2_3018 0.59 -0.9 3,505,362 - Pf6N2E2_3018 0.59 +0.0 3,505,380 - Pf6N2E2_3018 0.62 +1.3 3,505,502 + Pf6N2E2_3018 0.83 -2.9 3,505,613 + -0.4 3,505,614 - +0.6 3,505,614 - -1.1 3,505,864 + Pf6N2E2_3019 0.23 +0.8 3,505,864 + Pf6N2E2_3019 0.23 +3.1 3,505,865 - Pf6N2E2_3019 0.23 -1.3 3,505,865 - Pf6N2E2_3019 0.23 -1.3 3,505,865 - Pf6N2E2_3019 0.23 +0.6
Or see this region's nucleotide sequence