Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3016

Experiment: L-Serine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3013 and Pf6N2E2_3014 are separated by 9 nucleotidesPf6N2E2_3014 and Pf6N2E2_3015 overlap by 8 nucleotidesPf6N2E2_3015 and Pf6N2E2_3016 overlap by 1 nucleotidesPf6N2E2_3016 and Pf6N2E2_3017 are separated by 6 nucleotidesPf6N2E2_3017 and Pf6N2E2_3018 overlap by 4 nucleotidesPf6N2E2_3018 and Pf6N2E2_3019 are separated by 152 nucleotides Pf6N2E2_3013 - FIG032563: Phage tail protein D, at 3,501,110 to 3,502,126 _3013 Pf6N2E2_3014 - P2-like prophage tail protein X, at 3,502,136 to 3,502,348 _3014 Pf6N2E2_3015 - hypothetical protein, at 3,502,341 to 3,502,724 _3015 Pf6N2E2_3016 - Phage tail length tape-measure protein, at 3,502,724 to 3,504,901 _3016 Pf6N2E2_3017 - hypothetical protein, at 3,504,908 to 3,505,033 _3017 Pf6N2E2_3018 - FIG00955390: hypothetical protein, at 3,505,030 to 3,505,596 _3018 Pf6N2E2_3019 - FIG00954559: hypothetical protein, at 3,505,749 to 3,506,255 _3019 Position (kb) 3502 3503 3504 3505Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3502.217 kb on + strand, within Pf6N2E2_3014at 3502.217 kb on + strand, within Pf6N2E2_3014at 3502.299 kb on + strand, within Pf6N2E2_3014at 3502.543 kb on + strand, within Pf6N2E2_3015at 3502.544 kb on - strand, within Pf6N2E2_3015at 3502.544 kb on - strand, within Pf6N2E2_3015at 3502.544 kb on - strand, within Pf6N2E2_3015at 3502.729 kb on + strandat 3502.729 kb on + strandat 3502.730 kb on - strandat 3502.730 kb on - strandat 3502.866 kb on + strandat 3502.866 kb on + strandat 3502.867 kb on - strandat 3503.168 kb on + strand, within Pf6N2E2_3016at 3503.207 kb on + strand, within Pf6N2E2_3016at 3503.297 kb on - strand, within Pf6N2E2_3016at 3503.297 kb on - strand, within Pf6N2E2_3016at 3503.297 kb on - strand, within Pf6N2E2_3016at 3503.306 kb on + strand, within Pf6N2E2_3016at 3503.477 kb on + strand, within Pf6N2E2_3016at 3503.548 kb on + strand, within Pf6N2E2_3016at 3504.033 kb on - strand, within Pf6N2E2_3016at 3504.608 kb on + strand, within Pf6N2E2_3016at 3504.608 kb on + strand, within Pf6N2E2_3016at 3504.609 kb on - strand, within Pf6N2E2_3016at 3504.609 kb on - strand, within Pf6N2E2_3016at 3504.609 kb on - strand, within Pf6N2E2_3016at 3504.609 kb on - strand, within Pf6N2E2_3016at 3504.626 kb on - strand, within Pf6N2E2_3016at 3504.827 kb on - strandat 3504.835 kb on + strandat 3504.836 kb on - strandat 3504.836 kb on - strandat 3504.874 kb on - strandat 3504.874 kb on - strandat 3504.885 kb on + strandat 3504.886 kb on - strandat 3504.890 kb on - strandat 3504.890 kb on - strandat 3504.890 kb on - strandat 3504.909 kb on + strandat 3504.909 kb on + strandat 3504.910 kb on - strandat 3504.998 kb on + strand, within Pf6N2E2_3017at 3504.999 kb on - strand, within Pf6N2E2_3017at 3504.999 kb on - strand, within Pf6N2E2_3017at 3505.006 kb on - strand, within Pf6N2E2_3017at 3505.073 kb on - strandat 3505.291 kb on + strand, within Pf6N2E2_3018at 3505.291 kb on + strand, within Pf6N2E2_3018at 3505.292 kb on - strand, within Pf6N2E2_3018at 3505.292 kb on - strand, within Pf6N2E2_3018at 3505.361 kb on + strand, within Pf6N2E2_3018at 3505.361 kb on + strand, within Pf6N2E2_3018at 3505.361 kb on + strand, within Pf6N2E2_3018at 3505.362 kb on - strand, within Pf6N2E2_3018at 3505.362 kb on - strand, within Pf6N2E2_3018at 3505.380 kb on - strand, within Pf6N2E2_3018at 3505.502 kb on + strand, within Pf6N2E2_3018at 3505.613 kb on + strandat 3505.614 kb on - strandat 3505.614 kb on - strandat 3505.864 kb on + strand, within Pf6N2E2_3019at 3505.864 kb on + strand, within Pf6N2E2_3019at 3505.865 kb on - strand, within Pf6N2E2_3019at 3505.865 kb on - strand, within Pf6N2E2_3019at 3505.865 kb on - strand, within Pf6N2E2_3019

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Serine (N)
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3,502,217 + Pf6N2E2_3014 0.38 -1.3
3,502,217 + Pf6N2E2_3014 0.38 +1.2
3,502,299 + Pf6N2E2_3014 0.77 +0.2
3,502,543 + Pf6N2E2_3015 0.53 -0.4
3,502,544 - Pf6N2E2_3015 0.53 -1.5
3,502,544 - Pf6N2E2_3015 0.53 -0.4
3,502,544 - Pf6N2E2_3015 0.53 +1.0
3,502,729 + -0.4
3,502,729 + +0.6
3,502,730 - -0.1
3,502,730 - +0.4
3,502,866 + +1.1
3,502,866 + +1.2
3,502,867 - +0.9
3,503,168 + Pf6N2E2_3016 0.20 -0.2
3,503,207 + Pf6N2E2_3016 0.22 +2.0
3,503,297 - Pf6N2E2_3016 0.26 -0.0
3,503,297 - Pf6N2E2_3016 0.26 +1.8
3,503,297 - Pf6N2E2_3016 0.26 -1.1
3,503,306 + Pf6N2E2_3016 0.27 +0.3
3,503,477 + Pf6N2E2_3016 0.35 -0.4
3,503,548 + Pf6N2E2_3016 0.38 +0.4
3,504,033 - Pf6N2E2_3016 0.60 +0.9
3,504,608 + Pf6N2E2_3016 0.87 -0.7
3,504,608 + Pf6N2E2_3016 0.87 +1.1
3,504,609 - Pf6N2E2_3016 0.87 +1.5
3,504,609 - Pf6N2E2_3016 0.87 +1.6
3,504,609 - Pf6N2E2_3016 0.87 +1.0
3,504,609 - Pf6N2E2_3016 0.87 -2.3
3,504,626 - Pf6N2E2_3016 0.87 +1.2
3,504,827 - -1.6
3,504,835 + -0.9
3,504,836 - -1.1
3,504,836 - -2.6
3,504,874 - -2.2
3,504,874 - +0.7
3,504,885 + +0.5
3,504,886 - -0.6
3,504,890 - +0.0
3,504,890 - -0.7
3,504,890 - +1.4
3,504,909 + +0.7
3,504,909 + +1.3
3,504,910 - +0.0
3,504,998 + Pf6N2E2_3017 0.71 +0.6
3,504,999 - Pf6N2E2_3017 0.72 -1.0
3,504,999 - Pf6N2E2_3017 0.72 -0.5
3,505,006 - Pf6N2E2_3017 0.78 +1.2
3,505,073 - -0.3
3,505,291 + Pf6N2E2_3018 0.46 -0.2
3,505,291 + Pf6N2E2_3018 0.46 +1.1
3,505,292 - Pf6N2E2_3018 0.46 -1.4
3,505,292 - Pf6N2E2_3018 0.46 +1.3
3,505,361 + Pf6N2E2_3018 0.58 +0.8
3,505,361 + Pf6N2E2_3018 0.58 +0.5
3,505,361 + Pf6N2E2_3018 0.58 +0.5
3,505,362 - Pf6N2E2_3018 0.59 -0.9
3,505,362 - Pf6N2E2_3018 0.59 +0.0
3,505,380 - Pf6N2E2_3018 0.62 +1.3
3,505,502 + Pf6N2E2_3018 0.83 -2.9
3,505,613 + -0.4
3,505,614 - +0.6
3,505,614 - -1.1
3,505,864 + Pf6N2E2_3019 0.23 +0.8
3,505,864 + Pf6N2E2_3019 0.23 +3.1
3,505,865 - Pf6N2E2_3019 0.23 -1.3
3,505,865 - Pf6N2E2_3019 0.23 -1.3
3,505,865 - Pf6N2E2_3019 0.23 +0.6

Or see this region's nucleotide sequence