Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2951 and Pf6N2E2_2952 are separated by 290 nucleotides Pf6N2E2_2952 and Pf6N2E2_2953 overlap by 2 nucleotides Pf6N2E2_2953 and Pf6N2E2_2954 are separated by 84 nucleotides
Pf6N2E2_2951 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 3,441,481 to 3,441,684
_2951
Pf6N2E2_2952 - Isochorismatase (EC 3.3.2.1), at 3,441,975 to 3,442,532
_2952
Pf6N2E2_2953 - hypothetical protein, at 3,442,531 to 3,442,653
_2953
Pf6N2E2_2954 - Penicillin acylase (EC 3.5.1.11), at 3,442,738 to 3,445,188
_2954
Position (kb)
3442
3443 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3441.714 kb on + strand at 3441.714 kb on + strand at 3441.714 kb on + strand at 3441.974 kb on - strand at 3441.974 kb on - strand at 3442.019 kb on - strand at 3442.019 kb on - strand at 3442.084 kb on + strand, within Pf6N2E2_2952 at 3442.085 kb on - strand, within Pf6N2E2_2952 at 3442.103 kb on + strand, within Pf6N2E2_2952 at 3442.103 kb on + strand, within Pf6N2E2_2952 at 3442.104 kb on - strand, within Pf6N2E2_2952 at 3442.104 kb on - strand, within Pf6N2E2_2952 at 3442.104 kb on - strand, within Pf6N2E2_2952 at 3442.410 kb on + strand, within Pf6N2E2_2952 at 3442.411 kb on - strand, within Pf6N2E2_2952 at 3442.411 kb on - strand, within Pf6N2E2_2952 at 3442.454 kb on + strand, within Pf6N2E2_2952 at 3442.454 kb on + strand, within Pf6N2E2_2952 at 3442.455 kb on - strand, within Pf6N2E2_2952 at 3442.524 kb on + strand at 3442.589 kb on - strand, within Pf6N2E2_2953 at 3442.651 kb on + strand at 3442.651 kb on + strand at 3442.651 kb on + strand at 3442.652 kb on - strand at 3442.652 kb on - strand at 3442.652 kb on - strand at 3442.652 kb on - strand at 3442.690 kb on + strand at 3442.691 kb on - strand at 3442.705 kb on - strand at 3442.705 kb on - strand at 3442.756 kb on - strand at 3442.973 kb on - strand at 3443.077 kb on + strand, within Pf6N2E2_2954 at 3443.077 kb on + strand, within Pf6N2E2_2954 at 3443.077 kb on + strand, within Pf6N2E2_2954 at 3443.077 kb on + strand, within Pf6N2E2_2954 at 3443.078 kb on - strand, within Pf6N2E2_2954 at 3443.119 kb on + strand, within Pf6N2E2_2954 at 3443.119 kb on + strand, within Pf6N2E2_2954 at 3443.119 kb on + strand, within Pf6N2E2_2954 at 3443.119 kb on + strand, within Pf6N2E2_2954 at 3443.120 kb on - strand, within Pf6N2E2_2954 at 3443.120 kb on - strand, within Pf6N2E2_2954 at 3443.120 kb on - strand, within Pf6N2E2_2954 at 3443.120 kb on - strand, within Pf6N2E2_2954 at 3443.120 kb on - strand, within Pf6N2E2_2954 at 3443.120 kb on - strand, within Pf6N2E2_2954 at 3443.120 kb on - strand, within Pf6N2E2_2954 at 3443.161 kb on + strand, within Pf6N2E2_2954 at 3443.162 kb on - strand, within Pf6N2E2_2954 at 3443.162 kb on - strand, within Pf6N2E2_2954 at 3443.183 kb on - strand, within Pf6N2E2_2954 at 3443.233 kb on + strand, within Pf6N2E2_2954 at 3443.644 kb on + strand, within Pf6N2E2_2954 at 3443.645 kb on - strand, within Pf6N2E2_2954
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 3,441,714 + +0.5 3,441,714 + +0.5 3,441,714 + -0.8 3,441,974 - +0.2 3,441,974 - +0.2 3,442,019 - +0.4 3,442,019 - -2.1 3,442,084 + Pf6N2E2_2952 0.20 -1.0 3,442,085 - Pf6N2E2_2952 0.20 +1.2 3,442,103 + Pf6N2E2_2952 0.23 +0.1 3,442,103 + Pf6N2E2_2952 0.23 -1.3 3,442,104 - Pf6N2E2_2952 0.23 -0.1 3,442,104 - Pf6N2E2_2952 0.23 -1.2 3,442,104 - Pf6N2E2_2952 0.23 -0.4 3,442,410 + Pf6N2E2_2952 0.78 +1.0 3,442,411 - Pf6N2E2_2952 0.78 +0.7 3,442,411 - Pf6N2E2_2952 0.78 -2.0 3,442,454 + Pf6N2E2_2952 0.86 -1.6 3,442,454 + Pf6N2E2_2952 0.86 +1.3 3,442,455 - Pf6N2E2_2952 0.86 +0.4 3,442,524 + +0.4 3,442,589 - Pf6N2E2_2953 0.47 +0.9 3,442,651 + -0.3 3,442,651 + +0.5 3,442,651 + -0.9 3,442,652 - +0.1 3,442,652 - -2.3 3,442,652 - +0.2 3,442,652 - +1.3 3,442,690 + +0.7 3,442,691 - -0.1 3,442,705 - -0.7 3,442,705 - -0.7 3,442,756 - +0.0 3,442,973 - +1.8 3,443,077 + Pf6N2E2_2954 0.14 +1.1 3,443,077 + Pf6N2E2_2954 0.14 -0.4 3,443,077 + Pf6N2E2_2954 0.14 +0.7 3,443,077 + Pf6N2E2_2954 0.14 +1.1 3,443,078 - Pf6N2E2_2954 0.14 +0.6 3,443,119 + Pf6N2E2_2954 0.16 +0.3 3,443,119 + Pf6N2E2_2954 0.16 +2.3 3,443,119 + Pf6N2E2_2954 0.16 +1.3 3,443,119 + Pf6N2E2_2954 0.16 +0.8 3,443,120 - Pf6N2E2_2954 0.16 +0.1 3,443,120 - Pf6N2E2_2954 0.16 +0.1 3,443,120 - Pf6N2E2_2954 0.16 +1.2 3,443,120 - Pf6N2E2_2954 0.16 +0.3 3,443,120 - Pf6N2E2_2954 0.16 +0.7 3,443,120 - Pf6N2E2_2954 0.16 -1.6 3,443,120 - Pf6N2E2_2954 0.16 -0.1 3,443,161 + Pf6N2E2_2954 0.17 -0.3 3,443,162 - Pf6N2E2_2954 0.17 +1.5 3,443,162 - Pf6N2E2_2954 0.17 +1.8 3,443,183 - Pf6N2E2_2954 0.18 -0.8 3,443,233 + Pf6N2E2_2954 0.20 +0.5 3,443,644 + Pf6N2E2_2954 0.37 +0.0 3,443,645 - Pf6N2E2_2954 0.37 -1.9
Or see this region's nucleotide sequence