Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2953

Experiment: L-Serine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2951 and Pf6N2E2_2952 are separated by 290 nucleotidesPf6N2E2_2952 and Pf6N2E2_2953 overlap by 2 nucleotidesPf6N2E2_2953 and Pf6N2E2_2954 are separated by 84 nucleotides Pf6N2E2_2951 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 3,441,481 to 3,441,684 _2951 Pf6N2E2_2952 - Isochorismatase (EC 3.3.2.1), at 3,441,975 to 3,442,532 _2952 Pf6N2E2_2953 - hypothetical protein, at 3,442,531 to 3,442,653 _2953 Pf6N2E2_2954 - Penicillin acylase (EC 3.5.1.11), at 3,442,738 to 3,445,188 _2954 Position (kb) 3442 3443Strain fitness (log2 ratio) -2 -1 0 1 2at 3441.714 kb on + strandat 3441.714 kb on + strandat 3441.714 kb on + strandat 3441.974 kb on - strandat 3441.974 kb on - strandat 3442.019 kb on - strandat 3442.019 kb on - strandat 3442.084 kb on + strand, within Pf6N2E2_2952at 3442.085 kb on - strand, within Pf6N2E2_2952at 3442.103 kb on + strand, within Pf6N2E2_2952at 3442.103 kb on + strand, within Pf6N2E2_2952at 3442.104 kb on - strand, within Pf6N2E2_2952at 3442.104 kb on - strand, within Pf6N2E2_2952at 3442.104 kb on - strand, within Pf6N2E2_2952at 3442.410 kb on + strand, within Pf6N2E2_2952at 3442.411 kb on - strand, within Pf6N2E2_2952at 3442.411 kb on - strand, within Pf6N2E2_2952at 3442.454 kb on + strand, within Pf6N2E2_2952at 3442.454 kb on + strand, within Pf6N2E2_2952at 3442.455 kb on - strand, within Pf6N2E2_2952at 3442.524 kb on + strandat 3442.589 kb on - strand, within Pf6N2E2_2953at 3442.651 kb on + strandat 3442.651 kb on + strandat 3442.651 kb on + strandat 3442.652 kb on - strandat 3442.652 kb on - strandat 3442.652 kb on - strandat 3442.652 kb on - strandat 3442.690 kb on + strandat 3442.691 kb on - strandat 3442.705 kb on - strandat 3442.705 kb on - strandat 3442.756 kb on - strandat 3442.973 kb on - strandat 3443.077 kb on + strand, within Pf6N2E2_2954at 3443.077 kb on + strand, within Pf6N2E2_2954at 3443.077 kb on + strand, within Pf6N2E2_2954at 3443.077 kb on + strand, within Pf6N2E2_2954at 3443.078 kb on - strand, within Pf6N2E2_2954at 3443.119 kb on + strand, within Pf6N2E2_2954at 3443.119 kb on + strand, within Pf6N2E2_2954at 3443.119 kb on + strand, within Pf6N2E2_2954at 3443.119 kb on + strand, within Pf6N2E2_2954at 3443.120 kb on - strand, within Pf6N2E2_2954at 3443.120 kb on - strand, within Pf6N2E2_2954at 3443.120 kb on - strand, within Pf6N2E2_2954at 3443.120 kb on - strand, within Pf6N2E2_2954at 3443.120 kb on - strand, within Pf6N2E2_2954at 3443.120 kb on - strand, within Pf6N2E2_2954at 3443.120 kb on - strand, within Pf6N2E2_2954at 3443.161 kb on + strand, within Pf6N2E2_2954at 3443.162 kb on - strand, within Pf6N2E2_2954at 3443.162 kb on - strand, within Pf6N2E2_2954at 3443.183 kb on - strand, within Pf6N2E2_2954at 3443.233 kb on + strand, within Pf6N2E2_2954at 3443.644 kb on + strand, within Pf6N2E2_2954at 3443.645 kb on - strand, within Pf6N2E2_2954

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Serine (N)
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3,441,714 + +0.5
3,441,714 + +0.5
3,441,714 + -0.8
3,441,974 - +0.2
3,441,974 - +0.2
3,442,019 - +0.4
3,442,019 - -2.1
3,442,084 + Pf6N2E2_2952 0.20 -1.0
3,442,085 - Pf6N2E2_2952 0.20 +1.2
3,442,103 + Pf6N2E2_2952 0.23 +0.1
3,442,103 + Pf6N2E2_2952 0.23 -1.3
3,442,104 - Pf6N2E2_2952 0.23 -0.1
3,442,104 - Pf6N2E2_2952 0.23 -1.2
3,442,104 - Pf6N2E2_2952 0.23 -0.4
3,442,410 + Pf6N2E2_2952 0.78 +1.0
3,442,411 - Pf6N2E2_2952 0.78 +0.7
3,442,411 - Pf6N2E2_2952 0.78 -2.0
3,442,454 + Pf6N2E2_2952 0.86 -1.6
3,442,454 + Pf6N2E2_2952 0.86 +1.3
3,442,455 - Pf6N2E2_2952 0.86 +0.4
3,442,524 + +0.4
3,442,589 - Pf6N2E2_2953 0.47 +0.9
3,442,651 + -0.3
3,442,651 + +0.5
3,442,651 + -0.9
3,442,652 - +0.1
3,442,652 - -2.3
3,442,652 - +0.2
3,442,652 - +1.3
3,442,690 + +0.7
3,442,691 - -0.1
3,442,705 - -0.7
3,442,705 - -0.7
3,442,756 - +0.0
3,442,973 - +1.8
3,443,077 + Pf6N2E2_2954 0.14 +1.1
3,443,077 + Pf6N2E2_2954 0.14 -0.4
3,443,077 + Pf6N2E2_2954 0.14 +0.7
3,443,077 + Pf6N2E2_2954 0.14 +1.1
3,443,078 - Pf6N2E2_2954 0.14 +0.6
3,443,119 + Pf6N2E2_2954 0.16 +0.3
3,443,119 + Pf6N2E2_2954 0.16 +2.3
3,443,119 + Pf6N2E2_2954 0.16 +1.3
3,443,119 + Pf6N2E2_2954 0.16 +0.8
3,443,120 - Pf6N2E2_2954 0.16 +0.1
3,443,120 - Pf6N2E2_2954 0.16 +0.1
3,443,120 - Pf6N2E2_2954 0.16 +1.2
3,443,120 - Pf6N2E2_2954 0.16 +0.3
3,443,120 - Pf6N2E2_2954 0.16 +0.7
3,443,120 - Pf6N2E2_2954 0.16 -1.6
3,443,120 - Pf6N2E2_2954 0.16 -0.1
3,443,161 + Pf6N2E2_2954 0.17 -0.3
3,443,162 - Pf6N2E2_2954 0.17 +1.5
3,443,162 - Pf6N2E2_2954 0.17 +1.8
3,443,183 - Pf6N2E2_2954 0.18 -0.8
3,443,233 + Pf6N2E2_2954 0.20 +0.5
3,443,644 + Pf6N2E2_2954 0.37 +0.0
3,443,645 - Pf6N2E2_2954 0.37 -1.9

Or see this region's nucleotide sequence