Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2081 and Pf6N2E2_2082 are separated by 217 nucleotides Pf6N2E2_2082 and Pf6N2E2_2083 are separated by 59 nucleotides Pf6N2E2_2083 and Pf6N2E2_2084 are separated by 98 nucleotides
Pf6N2E2_2081 - putative exported protein, at 2,474,446 to 2,474,967
_2081
Pf6N2E2_2082 - Integral membrane protein, at 2,475,185 to 2,475,838
_2082
Pf6N2E2_2083 - hypothetical protein, at 2,475,898 to 2,476,179
_2083
Pf6N2E2_2084 - sensory box histidine kinase/response regulator, at 2,476,278 to 2,478,341
_2084
Position (kb)
2475
2476
2477 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2474.965 kb on + strand at 2475.072 kb on - strand at 2475.077 kb on - strand at 2475.094 kb on + strand at 2475.095 kb on - strand at 2475.095 kb on - strand at 2475.124 kb on - strand at 2475.124 kb on - strand at 2475.124 kb on - strand at 2475.124 kb on - strand at 2475.278 kb on + strand, within Pf6N2E2_2082 at 2475.330 kb on + strand, within Pf6N2E2_2082 at 2475.347 kb on + strand, within Pf6N2E2_2082 at 2475.347 kb on + strand, within Pf6N2E2_2082 at 2475.347 kb on + strand, within Pf6N2E2_2082 at 2475.348 kb on - strand, within Pf6N2E2_2082 at 2475.392 kb on + strand, within Pf6N2E2_2082 at 2475.392 kb on + strand, within Pf6N2E2_2082 at 2475.392 kb on + strand, within Pf6N2E2_2082 at 2475.393 kb on - strand, within Pf6N2E2_2082 at 2475.393 kb on - strand, within Pf6N2E2_2082 at 2475.474 kb on + strand, within Pf6N2E2_2082 at 2475.474 kb on + strand, within Pf6N2E2_2082 at 2475.491 kb on + strand, within Pf6N2E2_2082 at 2475.491 kb on + strand, within Pf6N2E2_2082 at 2475.492 kb on - strand, within Pf6N2E2_2082 at 2475.492 kb on - strand, within Pf6N2E2_2082 at 2475.492 kb on - strand, within Pf6N2E2_2082 at 2475.587 kb on + strand, within Pf6N2E2_2082 at 2475.587 kb on + strand, within Pf6N2E2_2082 at 2475.588 kb on - strand, within Pf6N2E2_2082 at 2475.588 kb on - strand, within Pf6N2E2_2082 at 2475.588 kb on - strand, within Pf6N2E2_2082 at 2475.588 kb on - strand, within Pf6N2E2_2082 at 2475.650 kb on + strand, within Pf6N2E2_2082 at 2475.662 kb on + strand, within Pf6N2E2_2082 at 2475.662 kb on + strand, within Pf6N2E2_2082 at 2475.671 kb on + strand, within Pf6N2E2_2082 at 2475.672 kb on - strand, within Pf6N2E2_2082 at 2475.672 kb on - strand, within Pf6N2E2_2082 at 2475.689 kb on + strand, within Pf6N2E2_2082 at 2475.690 kb on - strand, within Pf6N2E2_2082 at 2475.690 kb on - strand, within Pf6N2E2_2082 at 2475.690 kb on - strand, within Pf6N2E2_2082 at 2475.771 kb on + strand, within Pf6N2E2_2082 at 2475.771 kb on + strand, within Pf6N2E2_2082 at 2475.772 kb on - strand, within Pf6N2E2_2082 at 2475.772 kb on - strand, within Pf6N2E2_2082 at 2475.984 kb on + strand, within Pf6N2E2_2083 at 2475.984 kb on + strand, within Pf6N2E2_2083 at 2475.984 kb on + strand, within Pf6N2E2_2083 at 2475.984 kb on + strand, within Pf6N2E2_2083 at 2475.985 kb on - strand, within Pf6N2E2_2083 at 2476.180 kb on - strand at 2476.180 kb on - strand at 2476.226 kb on + strand at 2476.328 kb on - strand at 2476.711 kb on + strand, within Pf6N2E2_2084 at 2476.711 kb on + strand, within Pf6N2E2_2084 at 2476.711 kb on + strand, within Pf6N2E2_2084 at 2476.712 kb on - strand, within Pf6N2E2_2084 at 2476.712 kb on - strand, within Pf6N2E2_2084 at 2476.712 kb on - strand, within Pf6N2E2_2084 at 2476.719 kb on + strand, within Pf6N2E2_2084 at 2476.912 kb on + strand, within Pf6N2E2_2084 at 2476.912 kb on + strand, within Pf6N2E2_2084 at 2476.912 kb on + strand, within Pf6N2E2_2084 at 2476.912 kb on + strand, within Pf6N2E2_2084 at 2477.152 kb on + strand, within Pf6N2E2_2084 at 2477.152 kb on + strand, within Pf6N2E2_2084 at 2477.152 kb on + strand, within Pf6N2E2_2084 at 2477.152 kb on + strand, within Pf6N2E2_2084 at 2477.152 kb on + strand, within Pf6N2E2_2084 at 2477.153 kb on - strand, within Pf6N2E2_2084 at 2477.153 kb on - strand, within Pf6N2E2_2084 at 2477.153 kb on - strand, within Pf6N2E2_2084 at 2477.153 kb on - strand, within Pf6N2E2_2084 at 2477.153 kb on - strand, within Pf6N2E2_2084 at 2477.153 kb on - strand, within Pf6N2E2_2084 at 2477.153 kb on - strand, within Pf6N2E2_2084 at 2477.153 kb on - strand, within Pf6N2E2_2084 at 2477.153 kb on - strand, within Pf6N2E2_2084 at 2477.153 kb on - strand, within Pf6N2E2_2084 at 2477.170 kb on + strand, within Pf6N2E2_2084 at 2477.171 kb on - strand, within Pf6N2E2_2084
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 2,474,965 + +0.4 2,475,072 - +0.7 2,475,077 - -0.7 2,475,094 + +1.1 2,475,095 - +0.8 2,475,095 - +0.8 2,475,124 - -2.1 2,475,124 - +0.6 2,475,124 - -0.4 2,475,124 - -1.1 2,475,278 + Pf6N2E2_2082 0.14 -1.9 2,475,330 + Pf6N2E2_2082 0.22 +0.7 2,475,347 + Pf6N2E2_2082 0.25 -0.4 2,475,347 + Pf6N2E2_2082 0.25 +0.0 2,475,347 + Pf6N2E2_2082 0.25 +2.3 2,475,348 - Pf6N2E2_2082 0.25 -1.0 2,475,392 + Pf6N2E2_2082 0.32 +0.0 2,475,392 + Pf6N2E2_2082 0.32 -0.3 2,475,392 + Pf6N2E2_2082 0.32 -1.4 2,475,393 - Pf6N2E2_2082 0.32 +0.2 2,475,393 - Pf6N2E2_2082 0.32 -1.9 2,475,474 + Pf6N2E2_2082 0.44 -2.4 2,475,474 + Pf6N2E2_2082 0.44 -2.0 2,475,491 + Pf6N2E2_2082 0.47 -0.4 2,475,491 + Pf6N2E2_2082 0.47 -0.6 2,475,492 - Pf6N2E2_2082 0.47 +0.6 2,475,492 - Pf6N2E2_2082 0.47 +0.0 2,475,492 - Pf6N2E2_2082 0.47 -1.7 2,475,587 + Pf6N2E2_2082 0.61 +1.5 2,475,587 + Pf6N2E2_2082 0.61 -0.2 2,475,588 - Pf6N2E2_2082 0.62 +0.6 2,475,588 - Pf6N2E2_2082 0.62 -1.0 2,475,588 - Pf6N2E2_2082 0.62 +1.6 2,475,588 - Pf6N2E2_2082 0.62 -0.2 2,475,650 + Pf6N2E2_2082 0.71 -0.8 2,475,662 + Pf6N2E2_2082 0.73 -0.4 2,475,662 + Pf6N2E2_2082 0.73 -0.8 2,475,671 + Pf6N2E2_2082 0.74 +2.0 2,475,672 - Pf6N2E2_2082 0.74 +0.0 2,475,672 - Pf6N2E2_2082 0.74 +0.1 2,475,689 + Pf6N2E2_2082 0.77 +0.9 2,475,690 - Pf6N2E2_2082 0.77 +2.5 2,475,690 - Pf6N2E2_2082 0.77 +0.5 2,475,690 - Pf6N2E2_2082 0.77 +0.6 2,475,771 + Pf6N2E2_2082 0.90 -1.5 2,475,771 + Pf6N2E2_2082 0.90 +1.3 2,475,772 - Pf6N2E2_2082 0.90 +1.2 2,475,772 - Pf6N2E2_2082 0.90 -0.1 2,475,984 + Pf6N2E2_2083 0.30 -0.1 2,475,984 + Pf6N2E2_2083 0.30 -1.8 2,475,984 + Pf6N2E2_2083 0.30 -1.1 2,475,984 + Pf6N2E2_2083 0.30 -2.6 2,475,985 - Pf6N2E2_2083 0.31 -2.6 2,476,180 - +0.4 2,476,180 - +0.9 2,476,226 + -0.8 2,476,328 - -2.5 2,476,711 + Pf6N2E2_2084 0.21 +0.9 2,476,711 + Pf6N2E2_2084 0.21 -1.0 2,476,711 + Pf6N2E2_2084 0.21 +0.5 2,476,712 - Pf6N2E2_2084 0.21 +2.6 2,476,712 - Pf6N2E2_2084 0.21 +0.6 2,476,712 - Pf6N2E2_2084 0.21 -1.1 2,476,719 + Pf6N2E2_2084 0.21 -1.6 2,476,912 + Pf6N2E2_2084 0.31 +0.2 2,476,912 + Pf6N2E2_2084 0.31 +1.0 2,476,912 + Pf6N2E2_2084 0.31 -1.8 2,476,912 + Pf6N2E2_2084 0.31 -2.5 2,477,152 + Pf6N2E2_2084 0.42 -1.0 2,477,152 + Pf6N2E2_2084 0.42 -0.7 2,477,152 + Pf6N2E2_2084 0.42 -1.4 2,477,152 + Pf6N2E2_2084 0.42 +0.5 2,477,152 + Pf6N2E2_2084 0.42 +1.3 2,477,153 - Pf6N2E2_2084 0.42 -0.2 2,477,153 - Pf6N2E2_2084 0.42 -2.0 2,477,153 - Pf6N2E2_2084 0.42 -0.4 2,477,153 - Pf6N2E2_2084 0.42 -0.4 2,477,153 - Pf6N2E2_2084 0.42 +1.5 2,477,153 - Pf6N2E2_2084 0.42 +0.8 2,477,153 - Pf6N2E2_2084 0.42 -0.7 2,477,153 - Pf6N2E2_2084 0.42 -0.8 2,477,153 - Pf6N2E2_2084 0.42 -1.8 2,477,153 - Pf6N2E2_2084 0.42 +1.6 2,477,170 + Pf6N2E2_2084 0.43 -0.7 2,477,171 - Pf6N2E2_2084 0.43 -0.3
Or see this region's nucleotide sequence