Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1829 and Pf6N2E2_1830 overlap by 1 nucleotides Pf6N2E2_1830 and Pf6N2E2_1831 are separated by 17 nucleotides Pf6N2E2_1831 and Pf6N2E2_1832 overlap by 4 nucleotides
Pf6N2E2_1829 - Mesaconyl-CoA C1-C4 CoA transferase, at 2,166,500 to 2,167,717
_1829
Pf6N2E2_1830 - Methylsuccinyl-CoA dehydrogenase, predicted by (Erb et al, 2007), at 2,167,717 to 2,169,387
_1830
Pf6N2E2_1831 - Citrate lyase beta chain (EC 4.1.3.6), at 2,169,405 to 2,170,304
_1831
Pf6N2E2_1832 - COGs COG3777, at 2,170,301 to 2,171,158
_1832
Position (kb)
2167
2168
2169
2170 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2166.804 kb on - strand, within Pf6N2E2_1829 at 2166.902 kb on + strand, within Pf6N2E2_1829 at 2166.902 kb on + strand, within Pf6N2E2_1829 at 2166.903 kb on - strand, within Pf6N2E2_1829 at 2166.903 kb on - strand, within Pf6N2E2_1829 at 2166.903 kb on - strand, within Pf6N2E2_1829 at 2166.903 kb on - strand, within Pf6N2E2_1829 at 2166.938 kb on - strand, within Pf6N2E2_1829 at 2167.190 kb on + strand, within Pf6N2E2_1829 at 2167.312 kb on - strand, within Pf6N2E2_1829 at 2167.392 kb on + strand, within Pf6N2E2_1829 at 2167.393 kb on - strand, within Pf6N2E2_1829 at 2167.393 kb on - strand, within Pf6N2E2_1829 at 2167.393 kb on - strand, within Pf6N2E2_1829 at 2167.397 kb on + strand, within Pf6N2E2_1829 at 2167.398 kb on - strand, within Pf6N2E2_1829 at 2167.498 kb on + strand, within Pf6N2E2_1829 at 2168.006 kb on + strand, within Pf6N2E2_1830 at 2168.006 kb on + strand, within Pf6N2E2_1830 at 2168.007 kb on - strand, within Pf6N2E2_1830 at 2168.007 kb on - strand, within Pf6N2E2_1830 at 2168.007 kb on - strand, within Pf6N2E2_1830 at 2168.071 kb on + strand, within Pf6N2E2_1830 at 2168.072 kb on - strand, within Pf6N2E2_1830 at 2168.090 kb on + strand, within Pf6N2E2_1830 at 2168.091 kb on - strand, within Pf6N2E2_1830 at 2168.415 kb on + strand, within Pf6N2E2_1830 at 2168.419 kb on - strand, within Pf6N2E2_1830 at 2168.434 kb on + strand, within Pf6N2E2_1830 at 2168.434 kb on + strand, within Pf6N2E2_1830 at 2168.543 kb on + strand, within Pf6N2E2_1830 at 2168.836 kb on - strand, within Pf6N2E2_1830 at 2169.019 kb on + strand, within Pf6N2E2_1830 at 2169.019 kb on + strand, within Pf6N2E2_1830 at 2169.019 kb on + strand, within Pf6N2E2_1830 at 2169.020 kb on - strand, within Pf6N2E2_1830 at 2169.165 kb on + strand, within Pf6N2E2_1830 at 2169.245 kb on - strand at 2169.245 kb on - strand at 2169.387 kb on + strand at 2169.387 kb on + strand at 2169.387 kb on + strand at 2169.387 kb on + strand at 2169.388 kb on - strand at 2169.388 kb on - strand at 2169.410 kb on + strand at 2169.411 kb on - strand at 2169.411 kb on - strand at 2169.888 kb on + strand, within Pf6N2E2_1831 at 2169.904 kb on - strand, within Pf6N2E2_1831 at 2169.904 kb on - strand, within Pf6N2E2_1831 at 2170.088 kb on + strand, within Pf6N2E2_1831 at 2170.088 kb on + strand, within Pf6N2E2_1831 at 2170.088 kb on + strand, within Pf6N2E2_1831 at 2170.104 kb on + strand, within Pf6N2E2_1831 at 2170.104 kb on + strand, within Pf6N2E2_1831 at 2170.105 kb on - strand, within Pf6N2E2_1831 at 2170.105 kb on - strand, within Pf6N2E2_1831 at 2170.253 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 2,166,804 - Pf6N2E2_1829 0.25 -0.8 2,166,902 + Pf6N2E2_1829 0.33 +0.9 2,166,902 + Pf6N2E2_1829 0.33 +0.9 2,166,903 - Pf6N2E2_1829 0.33 -0.7 2,166,903 - Pf6N2E2_1829 0.33 +0.3 2,166,903 - Pf6N2E2_1829 0.33 +0.7 2,166,903 - Pf6N2E2_1829 0.33 +1.3 2,166,938 - Pf6N2E2_1829 0.36 +0.2 2,167,190 + Pf6N2E2_1829 0.57 +1.8 2,167,312 - Pf6N2E2_1829 0.67 -2.6 2,167,392 + Pf6N2E2_1829 0.73 +0.1 2,167,393 - Pf6N2E2_1829 0.73 -2.8 2,167,393 - Pf6N2E2_1829 0.73 -0.4 2,167,393 - Pf6N2E2_1829 0.73 -0.2 2,167,397 + Pf6N2E2_1829 0.74 +0.8 2,167,398 - Pf6N2E2_1829 0.74 -0.1 2,167,498 + Pf6N2E2_1829 0.82 -1.0 2,168,006 + Pf6N2E2_1830 0.17 +0.2 2,168,006 + Pf6N2E2_1830 0.17 +0.9 2,168,007 - Pf6N2E2_1830 0.17 -1.8 2,168,007 - Pf6N2E2_1830 0.17 +1.4 2,168,007 - Pf6N2E2_1830 0.17 -2.0 2,168,071 + Pf6N2E2_1830 0.21 +0.5 2,168,072 - Pf6N2E2_1830 0.21 +1.2 2,168,090 + Pf6N2E2_1830 0.22 -2.5 2,168,091 - Pf6N2E2_1830 0.22 +0.7 2,168,415 + Pf6N2E2_1830 0.42 -1.2 2,168,419 - Pf6N2E2_1830 0.42 -1.1 2,168,434 + Pf6N2E2_1830 0.43 -0.7 2,168,434 + Pf6N2E2_1830 0.43 -3.0 2,168,543 + Pf6N2E2_1830 0.49 -1.2 2,168,836 - Pf6N2E2_1830 0.67 -1.9 2,169,019 + Pf6N2E2_1830 0.78 -1.7 2,169,019 + Pf6N2E2_1830 0.78 +0.2 2,169,019 + Pf6N2E2_1830 0.78 +0.6 2,169,020 - Pf6N2E2_1830 0.78 -0.7 2,169,165 + Pf6N2E2_1830 0.87 +0.6 2,169,245 - +0.6 2,169,245 - +1.2 2,169,387 + -1.2 2,169,387 + +1.5 2,169,387 + +0.5 2,169,387 + +1.2 2,169,388 - +0.5 2,169,388 - -2.3 2,169,410 + -0.3 2,169,411 - -1.5 2,169,411 - +0.3 2,169,888 + Pf6N2E2_1831 0.54 -0.7 2,169,904 - Pf6N2E2_1831 0.55 -2.0 2,169,904 - Pf6N2E2_1831 0.55 -1.2 2,170,088 + Pf6N2E2_1831 0.76 +1.7 2,170,088 + Pf6N2E2_1831 0.76 +0.3 2,170,088 + Pf6N2E2_1831 0.76 +0.8 2,170,104 + Pf6N2E2_1831 0.78 +0.0 2,170,104 + Pf6N2E2_1831 0.78 -0.4 2,170,105 - Pf6N2E2_1831 0.78 -1.7 2,170,105 - Pf6N2E2_1831 0.78 -1.3 2,170,253 + -1.2
Or see this region's nucleotide sequence