Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1830

Experiment: L-Serine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1829 and Pf6N2E2_1830 overlap by 1 nucleotidesPf6N2E2_1830 and Pf6N2E2_1831 are separated by 17 nucleotidesPf6N2E2_1831 and Pf6N2E2_1832 overlap by 4 nucleotides Pf6N2E2_1829 - Mesaconyl-CoA C1-C4 CoA transferase, at 2,166,500 to 2,167,717 _1829 Pf6N2E2_1830 - Methylsuccinyl-CoA dehydrogenase, predicted by (Erb et al, 2007), at 2,167,717 to 2,169,387 _1830 Pf6N2E2_1831 - Citrate lyase beta chain (EC 4.1.3.6), at 2,169,405 to 2,170,304 _1831 Pf6N2E2_1832 - COGs COG3777, at 2,170,301 to 2,171,158 _1832 Position (kb) 2167 2168 2169 2170Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2166.804 kb on - strand, within Pf6N2E2_1829at 2166.902 kb on + strand, within Pf6N2E2_1829at 2166.902 kb on + strand, within Pf6N2E2_1829at 2166.903 kb on - strand, within Pf6N2E2_1829at 2166.903 kb on - strand, within Pf6N2E2_1829at 2166.903 kb on - strand, within Pf6N2E2_1829at 2166.903 kb on - strand, within Pf6N2E2_1829at 2166.938 kb on - strand, within Pf6N2E2_1829at 2167.190 kb on + strand, within Pf6N2E2_1829at 2167.312 kb on - strand, within Pf6N2E2_1829at 2167.392 kb on + strand, within Pf6N2E2_1829at 2167.393 kb on - strand, within Pf6N2E2_1829at 2167.393 kb on - strand, within Pf6N2E2_1829at 2167.393 kb on - strand, within Pf6N2E2_1829at 2167.397 kb on + strand, within Pf6N2E2_1829at 2167.398 kb on - strand, within Pf6N2E2_1829at 2167.498 kb on + strand, within Pf6N2E2_1829at 2168.006 kb on + strand, within Pf6N2E2_1830at 2168.006 kb on + strand, within Pf6N2E2_1830at 2168.007 kb on - strand, within Pf6N2E2_1830at 2168.007 kb on - strand, within Pf6N2E2_1830at 2168.007 kb on - strand, within Pf6N2E2_1830at 2168.071 kb on + strand, within Pf6N2E2_1830at 2168.072 kb on - strand, within Pf6N2E2_1830at 2168.090 kb on + strand, within Pf6N2E2_1830at 2168.091 kb on - strand, within Pf6N2E2_1830at 2168.415 kb on + strand, within Pf6N2E2_1830at 2168.419 kb on - strand, within Pf6N2E2_1830at 2168.434 kb on + strand, within Pf6N2E2_1830at 2168.434 kb on + strand, within Pf6N2E2_1830at 2168.543 kb on + strand, within Pf6N2E2_1830at 2168.836 kb on - strand, within Pf6N2E2_1830at 2169.019 kb on + strand, within Pf6N2E2_1830at 2169.019 kb on + strand, within Pf6N2E2_1830at 2169.019 kb on + strand, within Pf6N2E2_1830at 2169.020 kb on - strand, within Pf6N2E2_1830at 2169.165 kb on + strand, within Pf6N2E2_1830at 2169.245 kb on - strandat 2169.245 kb on - strandat 2169.387 kb on + strandat 2169.387 kb on + strandat 2169.387 kb on + strandat 2169.387 kb on + strandat 2169.388 kb on - strandat 2169.388 kb on - strandat 2169.410 kb on + strandat 2169.411 kb on - strandat 2169.411 kb on - strandat 2169.888 kb on + strand, within Pf6N2E2_1831at 2169.904 kb on - strand, within Pf6N2E2_1831at 2169.904 kb on - strand, within Pf6N2E2_1831at 2170.088 kb on + strand, within Pf6N2E2_1831at 2170.088 kb on + strand, within Pf6N2E2_1831at 2170.088 kb on + strand, within Pf6N2E2_1831at 2170.104 kb on + strand, within Pf6N2E2_1831at 2170.104 kb on + strand, within Pf6N2E2_1831at 2170.105 kb on - strand, within Pf6N2E2_1831at 2170.105 kb on - strand, within Pf6N2E2_1831at 2170.253 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Serine (N)
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2,166,804 - Pf6N2E2_1829 0.25 -0.8
2,166,902 + Pf6N2E2_1829 0.33 +0.9
2,166,902 + Pf6N2E2_1829 0.33 +0.9
2,166,903 - Pf6N2E2_1829 0.33 -0.7
2,166,903 - Pf6N2E2_1829 0.33 +0.3
2,166,903 - Pf6N2E2_1829 0.33 +0.7
2,166,903 - Pf6N2E2_1829 0.33 +1.3
2,166,938 - Pf6N2E2_1829 0.36 +0.2
2,167,190 + Pf6N2E2_1829 0.57 +1.8
2,167,312 - Pf6N2E2_1829 0.67 -2.6
2,167,392 + Pf6N2E2_1829 0.73 +0.1
2,167,393 - Pf6N2E2_1829 0.73 -2.8
2,167,393 - Pf6N2E2_1829 0.73 -0.4
2,167,393 - Pf6N2E2_1829 0.73 -0.2
2,167,397 + Pf6N2E2_1829 0.74 +0.8
2,167,398 - Pf6N2E2_1829 0.74 -0.1
2,167,498 + Pf6N2E2_1829 0.82 -1.0
2,168,006 + Pf6N2E2_1830 0.17 +0.2
2,168,006 + Pf6N2E2_1830 0.17 +0.9
2,168,007 - Pf6N2E2_1830 0.17 -1.8
2,168,007 - Pf6N2E2_1830 0.17 +1.4
2,168,007 - Pf6N2E2_1830 0.17 -2.0
2,168,071 + Pf6N2E2_1830 0.21 +0.5
2,168,072 - Pf6N2E2_1830 0.21 +1.2
2,168,090 + Pf6N2E2_1830 0.22 -2.5
2,168,091 - Pf6N2E2_1830 0.22 +0.7
2,168,415 + Pf6N2E2_1830 0.42 -1.2
2,168,419 - Pf6N2E2_1830 0.42 -1.1
2,168,434 + Pf6N2E2_1830 0.43 -0.7
2,168,434 + Pf6N2E2_1830 0.43 -3.0
2,168,543 + Pf6N2E2_1830 0.49 -1.2
2,168,836 - Pf6N2E2_1830 0.67 -1.9
2,169,019 + Pf6N2E2_1830 0.78 -1.7
2,169,019 + Pf6N2E2_1830 0.78 +0.2
2,169,019 + Pf6N2E2_1830 0.78 +0.6
2,169,020 - Pf6N2E2_1830 0.78 -0.7
2,169,165 + Pf6N2E2_1830 0.87 +0.6
2,169,245 - +0.6
2,169,245 - +1.2
2,169,387 + -1.2
2,169,387 + +1.5
2,169,387 + +0.5
2,169,387 + +1.2
2,169,388 - +0.5
2,169,388 - -2.3
2,169,410 + -0.3
2,169,411 - -1.5
2,169,411 - +0.3
2,169,888 + Pf6N2E2_1831 0.54 -0.7
2,169,904 - Pf6N2E2_1831 0.55 -2.0
2,169,904 - Pf6N2E2_1831 0.55 -1.2
2,170,088 + Pf6N2E2_1831 0.76 +1.7
2,170,088 + Pf6N2E2_1831 0.76 +0.3
2,170,088 + Pf6N2E2_1831 0.76 +0.8
2,170,104 + Pf6N2E2_1831 0.78 +0.0
2,170,104 + Pf6N2E2_1831 0.78 -0.4
2,170,105 - Pf6N2E2_1831 0.78 -1.7
2,170,105 - Pf6N2E2_1831 0.78 -1.3
2,170,253 + -1.2

Or see this region's nucleotide sequence