Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1463 and Pf6N2E2_1464 are separated by 308 nucleotides Pf6N2E2_1464 and Pf6N2E2_1465 are separated by 99 nucleotides Pf6N2E2_1465 and Pf6N2E2_1466 are separated by 429 nucleotides Pf6N2E2_1466 and Pf6N2E2_1467 are separated by 20 nucleotides
Pf6N2E2_1463 - Isochorismate pyruvate-lyase (EC 4.-.-.-), at 1,711,658 to 1,711,936
_1463
Pf6N2E2_1464 - hypothetical protein, at 1,712,245 to 1,712,763
_1464
Pf6N2E2_1465 - hypothetical protein, at 1,712,863 to 1,714,203
_1465
Pf6N2E2_1466 - NAD-dependent formate dehydrogenase delta subunit, at 1,714,633 to 1,714,830
_1466
Pf6N2E2_1467 - NAD-dependent formate dehydrogenase alpha subunit, at 1,714,851 to 1,717,727
_1467
Position (kb)
1712
1713
1714
1715 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1712.225 kb on + strand at 1712.226 kb on - strand at 1712.265 kb on + strand at 1712.266 kb on - strand at 1712.266 kb on - strand at 1712.266 kb on - strand at 1712.527 kb on + strand, within Pf6N2E2_1464 at 1712.632 kb on + strand, within Pf6N2E2_1464 at 1712.632 kb on + strand, within Pf6N2E2_1464 at 1712.633 kb on - strand, within Pf6N2E2_1464 at 1712.633 kb on - strand, within Pf6N2E2_1464 at 1712.633 kb on - strand, within Pf6N2E2_1464 at 1712.677 kb on + strand, within Pf6N2E2_1464 at 1712.746 kb on + strand at 1712.746 kb on + strand at 1712.746 kb on + strand at 1712.747 kb on - strand at 1712.779 kb on + strand at 1712.780 kb on - strand at 1712.780 kb on - strand at 1712.937 kb on - strand at 1712.937 kb on - strand at 1712.937 kb on - strand at 1712.937 kb on - strand at 1712.937 kb on - strand at 1712.946 kb on + strand at 1712.967 kb on - strand at 1712.998 kb on - strand, within Pf6N2E2_1465 at 1713.009 kb on + strand, within Pf6N2E2_1465 at 1713.056 kb on + strand, within Pf6N2E2_1465 at 1713.066 kb on - strand, within Pf6N2E2_1465 at 1713.096 kb on + strand, within Pf6N2E2_1465 at 1713.097 kb on - strand, within Pf6N2E2_1465 at 1713.167 kb on - strand, within Pf6N2E2_1465 at 1713.543 kb on + strand, within Pf6N2E2_1465 at 1713.563 kb on + strand, within Pf6N2E2_1465 at 1713.564 kb on - strand, within Pf6N2E2_1465 at 1713.570 kb on - strand, within Pf6N2E2_1465 at 1713.570 kb on - strand, within Pf6N2E2_1465 at 1713.611 kb on + strand, within Pf6N2E2_1465 at 1713.611 kb on + strand, within Pf6N2E2_1465 at 1713.612 kb on - strand, within Pf6N2E2_1465 at 1713.645 kb on + strand, within Pf6N2E2_1465 at 1713.706 kb on - strand, within Pf6N2E2_1465 at 1713.755 kb on - strand, within Pf6N2E2_1465 at 1713.915 kb on - strand, within Pf6N2E2_1465 at 1713.976 kb on + strand, within Pf6N2E2_1465 at 1713.976 kb on + strand, within Pf6N2E2_1465 at 1713.976 kb on + strand, within Pf6N2E2_1465 at 1713.976 kb on + strand, within Pf6N2E2_1465 at 1714.000 kb on - strand, within Pf6N2E2_1465 at 1714.000 kb on - strand, within Pf6N2E2_1465 at 1714.074 kb on + strand at 1714.074 kb on + strand at 1714.074 kb on + strand at 1714.075 kb on - strand at 1714.075 kb on - strand at 1714.075 kb on - strand at 1714.075 kb on - strand at 1714.079 kb on + strand at 1714.079 kb on + strand at 1714.079 kb on + strand at 1714.080 kb on - strand at 1714.080 kb on - strand at 1714.080 kb on - strand at 1714.080 kb on - strand at 1714.094 kb on + strand at 1714.094 kb on + strand at 1714.094 kb on + strand at 1714.095 kb on - strand at 1714.448 kb on - strand at 1714.448 kb on - strand at 1714.459 kb on + strand at 1714.459 kb on + strand at 1714.459 kb on + strand at 1714.460 kb on - strand at 1714.460 kb on - strand at 1714.487 kb on - strand at 1714.611 kb on - strand at 1714.624 kb on + strand at 1714.624 kb on + strand at 1714.635 kb on - strand at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.809 kb on - strand, within Pf6N2E2_1466 at 1714.809 kb on - strand, within Pf6N2E2_1466 at 1714.809 kb on - strand, within Pf6N2E2_1466 at 1714.834 kb on + strand at 1714.987 kb on + strand at 1714.987 kb on + strand at 1714.987 kb on + strand at 1714.987 kb on + strand at 1714.988 kb on - strand at 1714.988 kb on - strand at 1715.068 kb on + strand at 1715.069 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 1,712,225 + -0.1 1,712,226 - -1.5 1,712,265 + +0.4 1,712,266 - -0.6 1,712,266 - +2.5 1,712,266 - +2.0 1,712,527 + Pf6N2E2_1464 0.54 -1.4 1,712,632 + Pf6N2E2_1464 0.75 -1.6 1,712,632 + Pf6N2E2_1464 0.75 +0.9 1,712,633 - Pf6N2E2_1464 0.75 +2.4 1,712,633 - Pf6N2E2_1464 0.75 -0.9 1,712,633 - Pf6N2E2_1464 0.75 +1.4 1,712,677 + Pf6N2E2_1464 0.83 +1.4 1,712,746 + -1.2 1,712,746 + -1.1 1,712,746 + -0.5 1,712,747 - -0.3 1,712,779 + +1.3 1,712,780 - +0.7 1,712,780 - +0.5 1,712,937 - +1.6 1,712,937 - +0.9 1,712,937 - -0.0 1,712,937 - +0.1 1,712,937 - +0.1 1,712,946 + +0.5 1,712,967 - -0.7 1,712,998 - Pf6N2E2_1465 0.10 -1.0 1,713,009 + Pf6N2E2_1465 0.11 -0.3 1,713,056 + Pf6N2E2_1465 0.14 -0.4 1,713,066 - Pf6N2E2_1465 0.15 -0.8 1,713,096 + Pf6N2E2_1465 0.17 -1.0 1,713,097 - Pf6N2E2_1465 0.17 +0.1 1,713,167 - Pf6N2E2_1465 0.23 +2.0 1,713,543 + Pf6N2E2_1465 0.51 +0.9 1,713,563 + Pf6N2E2_1465 0.52 +0.5 1,713,564 - Pf6N2E2_1465 0.52 -0.3 1,713,570 - Pf6N2E2_1465 0.53 +0.5 1,713,570 - Pf6N2E2_1465 0.53 -0.7 1,713,611 + Pf6N2E2_1465 0.56 -2.0 1,713,611 + Pf6N2E2_1465 0.56 +1.2 1,713,612 - Pf6N2E2_1465 0.56 -0.6 1,713,645 + Pf6N2E2_1465 0.58 +1.9 1,713,706 - Pf6N2E2_1465 0.63 +1.1 1,713,755 - Pf6N2E2_1465 0.67 +0.5 1,713,915 - Pf6N2E2_1465 0.78 -1.3 1,713,976 + Pf6N2E2_1465 0.83 -0.6 1,713,976 + Pf6N2E2_1465 0.83 +0.1 1,713,976 + Pf6N2E2_1465 0.83 +0.6 1,713,976 + Pf6N2E2_1465 0.83 +2.3 1,714,000 - Pf6N2E2_1465 0.85 +1.7 1,714,000 - Pf6N2E2_1465 0.85 -0.6 1,714,074 + +0.1 1,714,074 + -1.3 1,714,074 + +0.4 1,714,075 - -0.1 1,714,075 - +0.7 1,714,075 - -1.0 1,714,075 - +0.3 1,714,079 + +0.7 1,714,079 + +2.4 1,714,079 + -1.8 1,714,080 - -3.0 1,714,080 - -1.1 1,714,080 - +0.5 1,714,080 - -1.2 1,714,094 + +0.7 1,714,094 + +0.2 1,714,094 + -1.6 1,714,095 - -2.2 1,714,448 - -0.3 1,714,448 - -1.2 1,714,459 + -1.1 1,714,459 + -2.2 1,714,459 + +0.9 1,714,460 - -2.2 1,714,460 - -1.8 1,714,487 - -1.8 1,714,611 - +0.4 1,714,624 + -0.6 1,714,624 + +0.3 1,714,635 - -0.2 1,714,785 + Pf6N2E2_1466 0.77 +0.5 1,714,785 + Pf6N2E2_1466 0.77 -0.1 1,714,785 + Pf6N2E2_1466 0.77 -0.1 1,714,785 + Pf6N2E2_1466 0.77 +0.6 1,714,785 + Pf6N2E2_1466 0.77 -2.5 1,714,785 + Pf6N2E2_1466 0.77 +0.0 1,714,786 - Pf6N2E2_1466 0.77 -2.8 1,714,786 - Pf6N2E2_1466 0.77 +0.1 1,714,786 - Pf6N2E2_1466 0.77 -1.5 1,714,786 - Pf6N2E2_1466 0.77 +0.7 1,714,786 - Pf6N2E2_1466 0.77 -0.5 1,714,786 - Pf6N2E2_1466 0.77 -1.5 1,714,786 - Pf6N2E2_1466 0.77 +1.9 1,714,809 - Pf6N2E2_1466 0.89 -0.6 1,714,809 - Pf6N2E2_1466 0.89 -0.6 1,714,809 - Pf6N2E2_1466 0.89 +1.4 1,714,834 + +3.3 1,714,987 + -0.9 1,714,987 + -2.0 1,714,987 + +0.3 1,714,987 + -0.5 1,714,988 - -0.3 1,714,988 - -1.3 1,715,068 + -0.1 1,715,069 - -1.0
Or see this region's nucleotide sequence