Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1266 and Pf6N2E2_6106 are separated by 163 nucleotides Pf6N2E2_6106 and Pf6N2E2_6107 are separated by 195 nucleotides Pf6N2E2_6107 and Pf6N2E2_1267 are separated by 393 nucleotides
Pf6N2E2_1266 - Integrase, at 1,491,279 to 1,492,598
_1266
Pf6N2E2_6106 - tRNA-Leu-TAA, at 1,492,762 to 1,492,845
_6106
Pf6N2E2_6107 - tRNA-Cys-GCA, at 1,493,041 to 1,493,111
_6107
Pf6N2E2_1267 - Membrane protein, putative, at 1,493,505 to 1,494,284
_1267
Position (kb)
1491
1492
1493 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1490.391 kb on + strand at 1490.391 kb on + strand at 1490.391 kb on + strand at 1490.391 kb on + strand at 1490.392 kb on - strand at 1490.394 kb on + strand at 1490.394 kb on + strand at 1490.425 kb on + strand at 1490.495 kb on + strand at 1490.496 kb on - strand at 1490.626 kb on + strand at 1490.627 kb on - strand at 1490.630 kb on + strand at 1490.638 kb on + strand at 1490.638 kb on + strand at 1490.638 kb on + strand at 1490.638 kb on + strand at 1490.639 kb on - strand at 1490.820 kb on - strand at 1490.842 kb on - strand at 1490.911 kb on - strand at 1490.975 kb on + strand at 1490.976 kb on - strand at 1490.976 kb on - strand at 1491.175 kb on - strand at 1491.223 kb on - strand at 1491.248 kb on - strand at 1491.281 kb on - strand at 1491.281 kb on - strand at 1491.295 kb on - strand at 1491.380 kb on - strand at 1491.439 kb on + strand, within Pf6N2E2_1266 at 1491.565 kb on - strand, within Pf6N2E2_1266 at 1491.585 kb on - strand, within Pf6N2E2_1266 at 1491.604 kb on - strand, within Pf6N2E2_1266 at 1491.631 kb on + strand, within Pf6N2E2_1266 at 1491.632 kb on - strand, within Pf6N2E2_1266 at 1491.634 kb on + strand, within Pf6N2E2_1266 at 1491.634 kb on + strand, within Pf6N2E2_1266 at 1491.634 kb on + strand, within Pf6N2E2_1266 at 1491.634 kb on + strand, within Pf6N2E2_1266 at 1491.635 kb on - strand, within Pf6N2E2_1266 at 1491.690 kb on + strand, within Pf6N2E2_1266 at 1491.691 kb on - strand, within Pf6N2E2_1266 at 1491.691 kb on - strand, within Pf6N2E2_1266 at 1491.691 kb on - strand, within Pf6N2E2_1266 at 1491.691 kb on - strand, within Pf6N2E2_1266 at 1491.874 kb on - strand, within Pf6N2E2_1266 at 1491.874 kb on - strand, within Pf6N2E2_1266 at 1491.885 kb on + strand, within Pf6N2E2_1266 at 1491.885 kb on + strand, within Pf6N2E2_1266 at 1491.885 kb on + strand, within Pf6N2E2_1266 at 1491.885 kb on + strand, within Pf6N2E2_1266 at 1491.886 kb on - strand, within Pf6N2E2_1266 at 1491.886 kb on - strand, within Pf6N2E2_1266 at 1491.899 kb on + strand, within Pf6N2E2_1266 at 1491.900 kb on - strand, within Pf6N2E2_1266 at 1492.025 kb on - strand, within Pf6N2E2_1266 at 1492.025 kb on - strand, within Pf6N2E2_1266 at 1492.025 kb on - strand, within Pf6N2E2_1266 at 1492.026 kb on + strand, within Pf6N2E2_1266 at 1492.026 kb on + strand, within Pf6N2E2_1266 at 1492.027 kb on - strand, within Pf6N2E2_1266 at 1492.027 kb on - strand, within Pf6N2E2_1266 at 1492.117 kb on - strand, within Pf6N2E2_1266 at 1492.325 kb on + strand, within Pf6N2E2_1266 at 1492.457 kb on + strand, within Pf6N2E2_1266 at 1492.457 kb on + strand, within Pf6N2E2_1266 at 1492.459 kb on + strand, within Pf6N2E2_1266 at 1492.532 kb on - strand at 1492.603 kb on - strand at 1492.603 kb on - strand at 1492.604 kb on + strand at 1492.627 kb on + strand at 1492.628 kb on - strand at 1492.628 kb on - strand at 1492.628 kb on - strand at 1492.628 kb on - strand at 1492.630 kb on - strand at 1492.639 kb on + strand at 1492.639 kb on + strand at 1492.645 kb on + strand at 1492.670 kb on + strand at 1492.671 kb on - strand at 1492.734 kb on - strand at 1492.754 kb on - strand at 1492.757 kb on - strand at 1492.922 kb on - strand at 1492.924 kb on - strand at 1492.924 kb on - strand at 1492.924 kb on - strand at 1492.926 kb on - strand at 1492.926 kb on - strand at 1492.926 kb on - strand at 1492.926 kb on - strand at 1492.926 kb on - strand at 1493.321 kb on + strand at 1493.322 kb on - strand at 1493.322 kb on - strand at 1493.322 kb on - strand at 1493.404 kb on - strand at 1493.456 kb on - strand at 1493.489 kb on + strand at 1493.489 kb on + strand at 1493.489 kb on + strand at 1493.493 kb on + strand at 1493.493 kb on + strand at 1493.494 kb on - strand at 1493.499 kb on + strand at 1493.500 kb on - strand at 1493.500 kb on - strand at 1493.543 kb on + strand at 1493.543 kb on + strand at 1493.544 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 1,490,391 + +0.4 1,490,391 + +3.3 1,490,391 + +0.5 1,490,391 + +1.7 1,490,392 - +2.1 1,490,394 + +0.4 1,490,394 + +0.2 1,490,425 + -0.2 1,490,495 + -0.6 1,490,496 - -1.7 1,490,626 + +1.7 1,490,627 - +1.7 1,490,630 + -1.0 1,490,638 + +1.7 1,490,638 + -1.9 1,490,638 + -0.9 1,490,638 + -0.4 1,490,639 - -0.7 1,490,820 - -2.5 1,490,842 - +0.5 1,490,911 - -0.9 1,490,975 + +1.3 1,490,976 - +1.3 1,490,976 - +0.1 1,491,175 - -2.0 1,491,223 - -1.6 1,491,248 - +0.5 1,491,281 - -2.0 1,491,281 - -0.4 1,491,295 - +0.9 1,491,380 - +0.7 1,491,439 + Pf6N2E2_1266 0.12 -0.6 1,491,565 - Pf6N2E2_1266 0.22 +1.2 1,491,585 - Pf6N2E2_1266 0.23 -1.7 1,491,604 - Pf6N2E2_1266 0.25 +0.1 1,491,631 + Pf6N2E2_1266 0.27 +1.9 1,491,632 - Pf6N2E2_1266 0.27 +0.1 1,491,634 + Pf6N2E2_1266 0.27 +1.7 1,491,634 + Pf6N2E2_1266 0.27 +0.7 1,491,634 + Pf6N2E2_1266 0.27 -1.1 1,491,634 + Pf6N2E2_1266 0.27 +1.6 1,491,635 - Pf6N2E2_1266 0.27 -0.1 1,491,690 + Pf6N2E2_1266 0.31 -0.4 1,491,691 - Pf6N2E2_1266 0.31 -0.8 1,491,691 - Pf6N2E2_1266 0.31 +0.9 1,491,691 - Pf6N2E2_1266 0.31 -2.3 1,491,691 - Pf6N2E2_1266 0.31 +0.1 1,491,874 - Pf6N2E2_1266 0.45 +0.9 1,491,874 - Pf6N2E2_1266 0.45 +1.1 1,491,885 + Pf6N2E2_1266 0.46 +0.5 1,491,885 + Pf6N2E2_1266 0.46 +1.5 1,491,885 + Pf6N2E2_1266 0.46 +0.4 1,491,885 + Pf6N2E2_1266 0.46 +1.5 1,491,886 - Pf6N2E2_1266 0.46 +1.3 1,491,886 - Pf6N2E2_1266 0.46 -0.9 1,491,899 + Pf6N2E2_1266 0.47 +1.0 1,491,900 - Pf6N2E2_1266 0.47 +1.0 1,492,025 - Pf6N2E2_1266 0.57 -2.0 1,492,025 - Pf6N2E2_1266 0.57 -1.5 1,492,025 - Pf6N2E2_1266 0.57 +1.0 1,492,026 + Pf6N2E2_1266 0.57 +0.1 1,492,026 + Pf6N2E2_1266 0.57 +2.5 1,492,027 - Pf6N2E2_1266 0.57 +1.0 1,492,027 - Pf6N2E2_1266 0.57 -0.3 1,492,117 - Pf6N2E2_1266 0.63 -0.9 1,492,325 + Pf6N2E2_1266 0.79 -0.0 1,492,457 + Pf6N2E2_1266 0.89 +0.3 1,492,457 + Pf6N2E2_1266 0.89 +2.1 1,492,459 + Pf6N2E2_1266 0.89 -0.6 1,492,532 - +0.5 1,492,603 - -0.6 1,492,603 - +1.5 1,492,604 + +1.1 1,492,627 + +1.6 1,492,628 - -2.0 1,492,628 - +2.6 1,492,628 - +1.0 1,492,628 - +1.0 1,492,630 - +0.7 1,492,639 + +1.7 1,492,639 + -0.3 1,492,645 + -0.9 1,492,670 + +0.6 1,492,671 - +0.3 1,492,734 - +0.1 1,492,754 - -1.4 1,492,757 - +1.3 1,492,922 - +2.0 1,492,924 - +0.2 1,492,924 - +0.3 1,492,924 - +2.3 1,492,926 - -0.3 1,492,926 - +0.3 1,492,926 - +0.6 1,492,926 - -0.3 1,492,926 - +1.0 1,493,321 + +0.6 1,493,322 - +0.7 1,493,322 - -1.2 1,493,322 - -0.1 1,493,404 - +0.8 1,493,456 - +1.1 1,493,489 + +1.2 1,493,489 + +1.0 1,493,489 + -1.6 1,493,493 + +0.9 1,493,493 + +0.1 1,493,494 - -0.7 1,493,499 + -1.1 1,493,500 - -0.3 1,493,500 - -0.5 1,493,543 + +0.7 1,493,543 + +0.1 1,493,544 - +0.1
Or see this region's nucleotide sequence