Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_121 and Pf6N2E2_122 are separated by 20 nucleotides Pf6N2E2_122 and Pf6N2E2_123 are separated by 0 nucleotides Pf6N2E2_123 and Pf6N2E2_124 overlap by 14 nucleotides
Pf6N2E2_121 - probable polysaccharide biosynthesis protein, at 168,810 to 169,832
_121
Pf6N2E2_122 - Tyrosine-protein kinase Wzc (EC 2.7.10.2), at 169,853 to 170,497
_122
Pf6N2E2_123 - Undecaprenyl-phosphate galactosephosphotransferase (EC 2.7.8.6), at 170,498 to 171,244
_123
Pf6N2E2_124 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 171,231 to 172,310
_124
Position (kb) 169
170
171 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 168.876 kb on + strand at 168.876 kb on + strand at 168.934 kb on + strand, within Pf6N2E2_121 at 168.934 kb on + strand, within Pf6N2E2_121 at 168.934 kb on + strand, within Pf6N2E2_121 at 168.935 kb on - strand, within Pf6N2E2_121 at 168.958 kb on + strand, within Pf6N2E2_121 at 169.003 kb on + strand, within Pf6N2E2_121 at 169.003 kb on + strand, within Pf6N2E2_121 at 169.129 kb on + strand, within Pf6N2E2_121 at 169.159 kb on - strand, within Pf6N2E2_121 at 169.159 kb on - strand, within Pf6N2E2_121 at 169.159 kb on - strand, within Pf6N2E2_121 at 169.159 kb on - strand, within Pf6N2E2_121 at 169.162 kb on + strand, within Pf6N2E2_121 at 169.162 kb on + strand, within Pf6N2E2_121 at 169.162 kb on + strand, within Pf6N2E2_121 at 169.162 kb on + strand, within Pf6N2E2_121 at 169.162 kb on + strand, within Pf6N2E2_121 at 169.163 kb on - strand, within Pf6N2E2_121 at 169.163 kb on - strand, within Pf6N2E2_121 at 169.163 kb on - strand, within Pf6N2E2_121 at 169.163 kb on - strand, within Pf6N2E2_121 at 169.163 kb on - strand, within Pf6N2E2_121 at 169.163 kb on - strand, within Pf6N2E2_121 at 169.163 kb on - strand, within Pf6N2E2_121 at 169.310 kb on - strand, within Pf6N2E2_121 at 169.627 kb on + strand, within Pf6N2E2_121 at 169.854 kb on + strand at 169.854 kb on + strand at 169.854 kb on + strand at 169.854 kb on + strand at 169.855 kb on - strand at 169.855 kb on - strand at 169.866 kb on + strand at 169.866 kb on + strand at 169.884 kb on + strand at 169.885 kb on - strand at 169.887 kb on + strand at 169.888 kb on - strand at 170.046 kb on + strand, within Pf6N2E2_122 at 170.079 kb on + strand, within Pf6N2E2_122 at 170.499 kb on + strand at 170.500 kb on - strand at 170.583 kb on + strand, within Pf6N2E2_123 at 170.583 kb on + strand, within Pf6N2E2_123 at 170.583 kb on + strand, within Pf6N2E2_123 at 170.583 kb on + strand, within Pf6N2E2_123 at 170.583 kb on + strand, within Pf6N2E2_123 at 170.584 kb on - strand, within Pf6N2E2_123 at 170.584 kb on - strand, within Pf6N2E2_123 at 170.584 kb on - strand, within Pf6N2E2_123 at 170.664 kb on + strand, within Pf6N2E2_123 at 170.664 kb on + strand, within Pf6N2E2_123 at 170.664 kb on + strand, within Pf6N2E2_123 at 170.697 kb on + strand, within Pf6N2E2_123 at 170.697 kb on + strand, within Pf6N2E2_123 at 170.703 kb on + strand, within Pf6N2E2_123 at 170.756 kb on + strand, within Pf6N2E2_123 at 170.756 kb on + strand, within Pf6N2E2_123 at 170.757 kb on - strand, within Pf6N2E2_123 at 170.757 kb on - strand, within Pf6N2E2_123 at 170.774 kb on + strand, within Pf6N2E2_123 at 170.931 kb on + strand, within Pf6N2E2_123 at 170.932 kb on - strand, within Pf6N2E2_123 at 171.038 kb on + strand, within Pf6N2E2_123 at 171.313 kb on + strand at 171.313 kb on + strand at 171.493 kb on + strand, within Pf6N2E2_124 at 171.493 kb on + strand, within Pf6N2E2_124 at 171.493 kb on + strand, within Pf6N2E2_124
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 168,876 + +1.3 168,876 + -1.7 168,934 + Pf6N2E2_121 0.12 +0.2 168,934 + Pf6N2E2_121 0.12 +1.2 168,934 + Pf6N2E2_121 0.12 +0.6 168,935 - Pf6N2E2_121 0.12 -1.3 168,958 + Pf6N2E2_121 0.14 +1.0 169,003 + Pf6N2E2_121 0.19 +2.2 169,003 + Pf6N2E2_121 0.19 +1.2 169,129 + Pf6N2E2_121 0.31 +2.6 169,159 - Pf6N2E2_121 0.34 -2.1 169,159 - Pf6N2E2_121 0.34 +0.8 169,159 - Pf6N2E2_121 0.34 +0.0 169,159 - Pf6N2E2_121 0.34 -0.7 169,162 + Pf6N2E2_121 0.34 +1.4 169,162 + Pf6N2E2_121 0.34 +1.0 169,162 + Pf6N2E2_121 0.34 -1.5 169,162 + Pf6N2E2_121 0.34 +0.5 169,162 + Pf6N2E2_121 0.34 -1.3 169,163 - Pf6N2E2_121 0.35 +0.9 169,163 - Pf6N2E2_121 0.35 -1.2 169,163 - Pf6N2E2_121 0.35 +3.0 169,163 - Pf6N2E2_121 0.35 -1.3 169,163 - Pf6N2E2_121 0.35 +1.5 169,163 - Pf6N2E2_121 0.35 -1.3 169,163 - Pf6N2E2_121 0.35 -2.5 169,310 - Pf6N2E2_121 0.49 -0.1 169,627 + Pf6N2E2_121 0.80 +1.9 169,854 + +1.4 169,854 + +1.2 169,854 + +0.2 169,854 + -1.7 169,855 - +0.9 169,855 - +1.2 169,866 + -0.2 169,866 + +1.5 169,884 + +0.1 169,885 - -1.2 169,887 + -0.8 169,888 - +1.3 170,046 + Pf6N2E2_122 0.30 -0.5 170,079 + Pf6N2E2_122 0.35 -2.1 170,499 + +1.8 170,500 - +1.7 170,583 + Pf6N2E2_123 0.11 -2.2 170,583 + Pf6N2E2_123 0.11 -1.0 170,583 + Pf6N2E2_123 0.11 -0.1 170,583 + Pf6N2E2_123 0.11 +0.3 170,583 + Pf6N2E2_123 0.11 -0.9 170,584 - Pf6N2E2_123 0.12 +1.7 170,584 - Pf6N2E2_123 0.12 -0.3 170,584 - Pf6N2E2_123 0.12 -0.7 170,664 + Pf6N2E2_123 0.22 -0.8 170,664 + Pf6N2E2_123 0.22 +1.4 170,664 + Pf6N2E2_123 0.22 -0.1 170,697 + Pf6N2E2_123 0.27 -0.3 170,697 + Pf6N2E2_123 0.27 -0.7 170,703 + Pf6N2E2_123 0.27 +0.9 170,756 + Pf6N2E2_123 0.35 -0.4 170,756 + Pf6N2E2_123 0.35 -2.4 170,757 - Pf6N2E2_123 0.35 -2.2 170,757 - Pf6N2E2_123 0.35 +1.3 170,774 + Pf6N2E2_123 0.37 -0.2 170,931 + Pf6N2E2_123 0.58 -0.7 170,932 - Pf6N2E2_123 0.58 -0.2 171,038 + Pf6N2E2_123 0.72 +0.3 171,313 + -0.0 171,313 + +0.6 171,493 + Pf6N2E2_124 0.24 -1.2 171,493 + Pf6N2E2_124 0.24 -1.0 171,493 + Pf6N2E2_124 0.24 +0.5
Or see this region's nucleotide sequence