Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1110 and Pf6N2E2_1111 are separated by 198 nucleotides Pf6N2E2_1111 and Pf6N2E2_1112 are separated by 2 nucleotides Pf6N2E2_1112 and Pf6N2E2_1113 are separated by 154 nucleotides
Pf6N2E2_1110 - FIG00964414: hypothetical protein, at 1,302,159 to 1,302,842
_1110
Pf6N2E2_1111 - cell wall degradation protein, at 1,303,041 to 1,304,567
_1111
Pf6N2E2_1112 - NAD(P)H oxidoreductase YRKL (EC 1.6.99.-) @ Putative NADPH-quinone reductase (modulator of drug activity B) @ Flavodoxin 2, at 1,304,570 to 1,305,199
_1112
Pf6N2E2_1113 - Transcriptional regulator, TetR family, at 1,305,354 to 1,305,905
_1113
Position (kb)
1303
1304
1305 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1302.045 kb on + strand at 1302.046 kb on - strand at 1302.046 kb on - strand at 1302.046 kb on - strand at 1302.046 kb on - strand at 1302.071 kb on - strand at 1302.071 kb on - strand at 1302.160 kb on + strand at 1302.183 kb on - strand at 1302.376 kb on + strand, within Pf6N2E2_1110 at 1302.476 kb on - strand, within Pf6N2E2_1110 at 1302.514 kb on + strand, within Pf6N2E2_1110 at 1302.514 kb on + strand, within Pf6N2E2_1110 at 1302.515 kb on - strand, within Pf6N2E2_1110 at 1302.515 kb on - strand, within Pf6N2E2_1110 at 1302.515 kb on - strand, within Pf6N2E2_1110 at 1302.591 kb on + strand, within Pf6N2E2_1110 at 1302.592 kb on - strand, within Pf6N2E2_1110 at 1302.951 kb on + strand at 1302.952 kb on - strand at 1302.998 kb on - strand at 1303.026 kb on + strand at 1303.026 kb on + strand at 1303.026 kb on + strand at 1303.027 kb on - strand at 1303.039 kb on + strand at 1303.040 kb on - strand at 1303.183 kb on - strand at 1303.288 kb on - strand, within Pf6N2E2_1111 at 1303.354 kb on - strand, within Pf6N2E2_1111 at 1303.530 kb on + strand, within Pf6N2E2_1111 at 1303.710 kb on + strand, within Pf6N2E2_1111 at 1303.710 kb on + strand, within Pf6N2E2_1111 at 1303.710 kb on + strand, within Pf6N2E2_1111 at 1303.711 kb on - strand, within Pf6N2E2_1111 at 1303.929 kb on + strand, within Pf6N2E2_1111 at 1303.930 kb on - strand, within Pf6N2E2_1111 at 1303.930 kb on - strand, within Pf6N2E2_1111 at 1304.265 kb on + strand, within Pf6N2E2_1111 at 1304.266 kb on - strand, within Pf6N2E2_1111 at 1304.505 kb on + strand at 1304.505 kb on + strand at 1304.506 kb on - strand at 1304.506 kb on - strand at 1304.506 kb on - strand at 1304.520 kb on + strand at 1304.520 kb on + strand at 1304.521 kb on - strand at 1304.766 kb on + strand, within Pf6N2E2_1112 at 1305.238 kb on + strand at 1305.239 kb on - strand at 1305.239 kb on - strand at 1305.239 kb on - strand at 1305.249 kb on + strand at 1305.453 kb on + strand, within Pf6N2E2_1113 at 1305.496 kb on - strand, within Pf6N2E2_1113 at 1305.496 kb on - strand, within Pf6N2E2_1113
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 1,302,045 + +1.7 1,302,046 - +0.6 1,302,046 - +0.4 1,302,046 - +0.4 1,302,046 - +0.7 1,302,071 - -2.1 1,302,071 - +1.0 1,302,160 + +0.3 1,302,183 - +1.5 1,302,376 + Pf6N2E2_1110 0.32 -0.5 1,302,476 - Pf6N2E2_1110 0.46 +3.2 1,302,514 + Pf6N2E2_1110 0.52 +1.2 1,302,514 + Pf6N2E2_1110 0.52 -0.1 1,302,515 - Pf6N2E2_1110 0.52 -0.8 1,302,515 - Pf6N2E2_1110 0.52 +0.5 1,302,515 - Pf6N2E2_1110 0.52 -0.6 1,302,591 + Pf6N2E2_1110 0.63 -2.7 1,302,592 - Pf6N2E2_1110 0.63 -0.1 1,302,951 + -1.6 1,302,952 - +0.2 1,302,998 - -0.3 1,303,026 + +3.1 1,303,026 + -1.0 1,303,026 + -0.3 1,303,027 - -1.1 1,303,039 + -0.1 1,303,040 - +1.0 1,303,183 - -0.3 1,303,288 - Pf6N2E2_1111 0.16 +1.4 1,303,354 - Pf6N2E2_1111 0.20 +2.1 1,303,530 + Pf6N2E2_1111 0.32 -1.3 1,303,710 + Pf6N2E2_1111 0.44 +1.2 1,303,710 + Pf6N2E2_1111 0.44 -1.0 1,303,710 + Pf6N2E2_1111 0.44 -2.6 1,303,711 - Pf6N2E2_1111 0.44 -1.8 1,303,929 + Pf6N2E2_1111 0.58 -1.6 1,303,930 - Pf6N2E2_1111 0.58 -1.8 1,303,930 - Pf6N2E2_1111 0.58 -2.1 1,304,265 + Pf6N2E2_1111 0.80 -2.1 1,304,266 - Pf6N2E2_1111 0.80 -1.6 1,304,505 + +0.8 1,304,505 + -0.4 1,304,506 - +0.3 1,304,506 - -1.1 1,304,506 - -0.1 1,304,520 + -2.5 1,304,520 + +1.2 1,304,521 - -1.7 1,304,766 + Pf6N2E2_1112 0.31 +2.3 1,305,238 + +0.9 1,305,239 - +0.8 1,305,239 - +0.4 1,305,239 - +0.8 1,305,249 + +1.8 1,305,453 + Pf6N2E2_1113 0.18 -0.6 1,305,496 - Pf6N2E2_1113 0.26 -1.1 1,305,496 - Pf6N2E2_1113 0.26 +0.8
Or see this region's nucleotide sequence