Experiment: L-Glutamic (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1547 and Pf6N2E2_1548 are separated by 103 nucleotides Pf6N2E2_1548 and Pf6N2E2_1549 are separated by 23 nucleotides Pf6N2E2_1549 and Pf6N2E2_1550 are separated by 148 nucleotides
Pf6N2E2_1547 - Permease of the drug/metabolite transporter (DMT) superfamily, at 1,806,431 to 1,807,387
_1547
Pf6N2E2_1548 - Lactoylglutathione lyase, at 1,807,491 to 1,807,877
_1548
Pf6N2E2_1549 - Transcriptional regulator, LysR family, at 1,807,901 to 1,808,833
_1549
Pf6N2E2_1550 - Multidrug resistance protein B, at 1,808,982 to 1,810,211
_1550
Position (kb)
1807
1808
1809 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1806.989 kb on + strand, within Pf6N2E2_1547 at 1806.990 kb on - strand, within Pf6N2E2_1547 at 1806.990 kb on - strand, within Pf6N2E2_1547 at 1806.990 kb on - strand, within Pf6N2E2_1547 at 1807.223 kb on + strand, within Pf6N2E2_1547 at 1807.223 kb on + strand, within Pf6N2E2_1547 at 1807.224 kb on - strand, within Pf6N2E2_1547 at 1807.414 kb on - strand at 1807.414 kb on - strand at 1807.414 kb on - strand at 1807.414 kb on - strand at 1807.414 kb on - strand at 1807.427 kb on + strand at 1807.498 kb on + strand at 1807.499 kb on - strand at 1807.499 kb on - strand at 1807.732 kb on - strand, within Pf6N2E2_1548 at 1807.804 kb on - strand, within Pf6N2E2_1548 at 1807.804 kb on - strand, within Pf6N2E2_1548 at 1807.806 kb on + strand, within Pf6N2E2_1548 at 1807.815 kb on + strand, within Pf6N2E2_1548 at 1807.816 kb on - strand, within Pf6N2E2_1548 at 1807.875 kb on + strand at 1807.875 kb on + strand at 1807.961 kb on + strand at 1807.962 kb on - strand at 1808.097 kb on - strand, within Pf6N2E2_1549 at 1808.244 kb on + strand, within Pf6N2E2_1549 at 1808.307 kb on + strand, within Pf6N2E2_1549 at 1808.308 kb on - strand, within Pf6N2E2_1549 at 1808.431 kb on + strand, within Pf6N2E2_1549 at 1808.432 kb on - strand, within Pf6N2E2_1549 at 1808.497 kb on + strand, within Pf6N2E2_1549 at 1808.623 kb on + strand, within Pf6N2E2_1549 at 1808.672 kb on - strand, within Pf6N2E2_1549 at 1808.672 kb on - strand, within Pf6N2E2_1549 at 1808.672 kb on - strand, within Pf6N2E2_1549 at 1808.798 kb on + strand at 1808.798 kb on + strand at 1808.798 kb on + strand at 1808.799 kb on - strand at 1808.799 kb on - strand at 1808.799 kb on - strand at 1808.952 kb on + strand at 1808.953 kb on - strand at 1808.954 kb on + strand at 1808.954 kb on + strand at 1808.955 kb on - strand at 1808.955 kb on - strand at 1808.955 kb on - strand at 1808.970 kb on + strand at 1808.970 kb on + strand at 1808.971 kb on - strand at 1808.971 kb on - strand at 1808.997 kb on - strand at 1808.997 kb on - strand at 1809.076 kb on - strand at 1809.087 kb on + strand at 1809.087 kb on + strand at 1809.088 kb on - strand at 1809.088 kb on - strand at 1809.174 kb on + strand, within Pf6N2E2_1550 at 1809.174 kb on + strand, within Pf6N2E2_1550 at 1809.288 kb on + strand, within Pf6N2E2_1550 at 1809.288 kb on + strand, within Pf6N2E2_1550 at 1809.288 kb on + strand, within Pf6N2E2_1550 at 1809.288 kb on + strand, within Pf6N2E2_1550 at 1809.289 kb on - strand, within Pf6N2E2_1550 at 1809.289 kb on - strand, within Pf6N2E2_1550 at 1809.318 kb on + strand, within Pf6N2E2_1550 at 1809.403 kb on - strand, within Pf6N2E2_1550 at 1809.484 kb on + strand, within Pf6N2E2_1550 at 1809.711 kb on + strand, within Pf6N2E2_1550 at 1809.712 kb on - strand, within Pf6N2E2_1550 at 1809.712 kb on - strand, within Pf6N2E2_1550 at 1809.737 kb on + strand, within Pf6N2E2_1550 at 1809.813 kb on + strand, within Pf6N2E2_1550 at 1809.814 kb on - strand, within Pf6N2E2_1550
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamic (N) remove 1,806,989 + Pf6N2E2_1547 0.58 +0.5 1,806,990 - Pf6N2E2_1547 0.58 -0.3 1,806,990 - Pf6N2E2_1547 0.58 +0.7 1,806,990 - Pf6N2E2_1547 0.58 +0.7 1,807,223 + Pf6N2E2_1547 0.83 +0.9 1,807,223 + Pf6N2E2_1547 0.83 +0.4 1,807,224 - Pf6N2E2_1547 0.83 +1.1 1,807,414 - +0.6 1,807,414 - -0.0 1,807,414 - +0.6 1,807,414 - -1.1 1,807,414 - +0.2 1,807,427 + +1.7 1,807,498 + -0.2 1,807,499 - -1.1 1,807,499 - +0.2 1,807,732 - Pf6N2E2_1548 0.62 +0.0 1,807,804 - Pf6N2E2_1548 0.81 +0.2 1,807,804 - Pf6N2E2_1548 0.81 +0.0 1,807,806 + Pf6N2E2_1548 0.81 +0.9 1,807,815 + Pf6N2E2_1548 0.84 +0.2 1,807,816 - Pf6N2E2_1548 0.84 -0.9 1,807,875 + +1.2 1,807,875 + +0.3 1,807,961 + -1.7 1,807,962 - +1.2 1,808,097 - Pf6N2E2_1549 0.21 -1.1 1,808,244 + Pf6N2E2_1549 0.37 -1.9 1,808,307 + Pf6N2E2_1549 0.44 -0.9 1,808,308 - Pf6N2E2_1549 0.44 +0.2 1,808,431 + Pf6N2E2_1549 0.57 -0.8 1,808,432 - Pf6N2E2_1549 0.57 -1.0 1,808,497 + Pf6N2E2_1549 0.64 -1.0 1,808,623 + Pf6N2E2_1549 0.77 +0.3 1,808,672 - Pf6N2E2_1549 0.83 -1.5 1,808,672 - Pf6N2E2_1549 0.83 +0.4 1,808,672 - Pf6N2E2_1549 0.83 -0.9 1,808,798 + +1.1 1,808,798 + +1.8 1,808,798 + +0.1 1,808,799 - -0.3 1,808,799 - -0.5 1,808,799 - -1.7 1,808,952 + -1.3 1,808,953 - -1.9 1,808,954 + +0.0 1,808,954 + +0.3 1,808,955 - -1.2 1,808,955 - +0.3 1,808,955 - +0.7 1,808,970 + +0.3 1,808,970 + +0.8 1,808,971 - -2.8 1,808,971 - +0.6 1,808,997 - +0.2 1,808,997 - -1.0 1,809,076 - +0.5 1,809,087 + +0.5 1,809,087 + +1.5 1,809,088 - -1.1 1,809,088 - +1.1 1,809,174 + Pf6N2E2_1550 0.16 +0.7 1,809,174 + Pf6N2E2_1550 0.16 -0.1 1,809,288 + Pf6N2E2_1550 0.25 -0.4 1,809,288 + Pf6N2E2_1550 0.25 +0.5 1,809,288 + Pf6N2E2_1550 0.25 -0.4 1,809,288 + Pf6N2E2_1550 0.25 -2.4 1,809,289 - Pf6N2E2_1550 0.25 -1.7 1,809,289 - Pf6N2E2_1550 0.25 -0.9 1,809,318 + Pf6N2E2_1550 0.27 +0.9 1,809,403 - Pf6N2E2_1550 0.34 +0.9 1,809,484 + Pf6N2E2_1550 0.41 +0.2 1,809,711 + Pf6N2E2_1550 0.59 -1.2 1,809,712 - Pf6N2E2_1550 0.59 -0.3 1,809,712 - Pf6N2E2_1550 0.59 +0.6 1,809,737 + Pf6N2E2_1550 0.61 +0.8 1,809,813 + Pf6N2E2_1550 0.68 -0.4 1,809,814 - Pf6N2E2_1550 0.68 +0.3
Or see this region's nucleotide sequence