Experiment: L-Glutamic (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1312 and Pf6N2E2_1313 are separated by 12 nucleotides Pf6N2E2_1313 and Pf6N2E2_1314 are separated by 14 nucleotides Pf6N2E2_1314 and Pf6N2E2_1315 are separated by 144 nucleotides Pf6N2E2_1315 and Pf6N2E2_1316 are separated by 252 nucleotides
Pf6N2E2_1312 - 4-hydroxyphenylacetate symporter, major facilitator superfamily (MFS), at 1,545,963 to 1,547,267
_1312
Pf6N2E2_1313 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-), at 1,547,280 to 1,548,083
_1313
Pf6N2E2_1314 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.n4), at 1,548,098 to 1,548,895
_1314
Pf6N2E2_1315 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68), at 1,549,040 to 1,549,462
_1315
Pf6N2E2_1316 - P-hydroxyphenylacetate hydroxylase C1:reductase component, at 1,549,715 to 1,550,656
_1316
Position (kb)
1548
1549 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1547.140 kb on - strand at 1547.190 kb on + strand at 1547.191 kb on - strand at 1547.191 kb on - strand at 1547.191 kb on - strand at 1547.191 kb on - strand at 1547.397 kb on + strand, within Pf6N2E2_1313 at 1547.398 kb on - strand, within Pf6N2E2_1313 at 1547.856 kb on + strand, within Pf6N2E2_1313 at 1547.856 kb on + strand, within Pf6N2E2_1313 at 1547.953 kb on - strand, within Pf6N2E2_1313 at 1548.182 kb on + strand, within Pf6N2E2_1314 at 1548.450 kb on + strand, within Pf6N2E2_1314 at 1548.450 kb on + strand, within Pf6N2E2_1314 at 1548.450 kb on + strand, within Pf6N2E2_1314 at 1548.451 kb on - strand, within Pf6N2E2_1314 at 1548.451 kb on - strand, within Pf6N2E2_1314 at 1548.678 kb on + strand, within Pf6N2E2_1314 at 1548.770 kb on + strand, within Pf6N2E2_1314 at 1548.770 kb on + strand, within Pf6N2E2_1314 at 1548.770 kb on + strand, within Pf6N2E2_1314 at 1548.771 kb on - strand, within Pf6N2E2_1314 at 1548.811 kb on + strand, within Pf6N2E2_1314 at 1548.891 kb on - strand at 1549.104 kb on - strand, within Pf6N2E2_1315 at 1549.125 kb on + strand, within Pf6N2E2_1315 at 1549.497 kb on + strand at 1549.508 kb on + strand at 1549.702 kb on + strand at 1549.703 kb on - strand at 1549.859 kb on + strand, within Pf6N2E2_1316
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamic (N) remove 1,547,140 - +0.9 1,547,190 + -0.4 1,547,191 - -1.9 1,547,191 - -1.4 1,547,191 - +0.3 1,547,191 - -0.1 1,547,397 + Pf6N2E2_1313 0.15 +1.2 1,547,398 - Pf6N2E2_1313 0.15 +1.6 1,547,856 + Pf6N2E2_1313 0.72 +0.7 1,547,856 + Pf6N2E2_1313 0.72 -0.6 1,547,953 - Pf6N2E2_1313 0.84 +0.1 1,548,182 + Pf6N2E2_1314 0.11 +1.5 1,548,450 + Pf6N2E2_1314 0.44 -0.8 1,548,450 + Pf6N2E2_1314 0.44 -1.4 1,548,450 + Pf6N2E2_1314 0.44 -0.8 1,548,451 - Pf6N2E2_1314 0.44 +1.2 1,548,451 - Pf6N2E2_1314 0.44 -0.0 1,548,678 + Pf6N2E2_1314 0.73 -1.3 1,548,770 + Pf6N2E2_1314 0.84 -0.2 1,548,770 + Pf6N2E2_1314 0.84 -0.2 1,548,770 + Pf6N2E2_1314 0.84 -1.1 1,548,771 - Pf6N2E2_1314 0.84 -3.0 1,548,811 + Pf6N2E2_1314 0.89 -0.4 1,548,891 - +0.9 1,549,104 - Pf6N2E2_1315 0.15 +0.3 1,549,125 + Pf6N2E2_1315 0.20 -0.7 1,549,497 + -0.1 1,549,508 + +1.0 1,549,702 + -1.3 1,549,703 - +0.5 1,549,859 + Pf6N2E2_1316 0.15 +1.0
Or see this region's nucleotide sequence