Experiment: Mc formate, casamino free, with 10mM L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08500 and MMP_RS08505 are separated by 114 nucleotides MMP_RS08505 and MMP_RS08510 are separated by 29 nucleotides MMP_RS08510 and MMP_RS08515 are separated by 13 nucleotides MMP_RS08515 and MMP_RS08520 are separated by 14 nucleotides MMP_RS08520 and MMP_RS08525 are separated by 38 nucleotides MMP_RS08525 and MMP_RS08530 are separated by 116 nucleotides MMP_RS08530 and MMP_RS08535 are separated by 122 nucleotides MMP_RS08535 and MMP_RS08540 are separated by 52 nucleotides MMP_RS08540 and MMP_RS08545 are separated by 159 nucleotides
MMP_RS08500: MMP_RS08500 - pyridoxal 5'-phosphate synthase glutaminase subunit PdxT, at 1,600,220 to 1,600,783
_RS08500
MMP_RS08505: MMP_RS08505 - tRNA-His, at 1,600,898 to 1,600,972
_RS08505
MMP_RS08510: MMP_RS08510 - tRNA-Leu, at 1,601,002 to 1,601,086
_RS08510
MMP_RS08515: MMP_RS08515 - tRNA-Glu, at 1,601,100 to 1,601,174
_RS08515
MMP_RS08520: MMP_RS08520 - tRNA-Ile, at 1,601,189 to 1,601,263
_RS08520
MMP_RS08525: MMP_RS08525 - tRNA-Asn, at 1,601,302 to 1,601,378
_RS08525
MMP_RS08530: MMP_RS08530 - tRNA-Phe, at 1,601,495 to 1,601,568
_RS08530
MMP_RS08535: MMP_RS08535 - bis-aminopropyl spermidine synthase family protein, at 1,601,691 to 1,602,863
_RS08535
MMP_RS08540: MMP_RS08540 - 30S ribosomal protein S8e, at 1,602,916 to 1,603,302
_RS08540
MMP_RS08545: MMP_RS08545 - aspartate carbamoyltransferase, at 1,603,462 to 1,604,370
_RS08545
Position (kb)
1601
1602
1603 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1600.808 kb on - strand at 1600.813 kb on + strand at 1600.824 kb on - strand at 1601.630 kb on - strand at 1601.633 kb on - strand at 1601.634 kb on + strand at 1601.650 kb on - strand at 1601.651 kb on + strand at 1601.655 kb on - strand at 1601.656 kb on + strand at 1601.658 kb on - strand at 1601.667 kb on + strand at 1601.671 kb on - strand at 1601.672 kb on + strand at 1601.672 kb on + strand at 1601.675 kb on + strand at 1602.002 kb on - strand, within MMP_RS08535 at 1602.003 kb on + strand, within MMP_RS08535 at 1602.009 kb on + strand, within MMP_RS08535 at 1602.009 kb on + strand, within MMP_RS08535 at 1602.303 kb on + strand, within MMP_RS08535 at 1602.353 kb on - strand, within MMP_RS08535 at 1602.354 kb on + strand, within MMP_RS08535 at 1602.354 kb on + strand, within MMP_RS08535 at 1602.436 kb on + strand, within MMP_RS08535 at 1602.436 kb on + strand, within MMP_RS08535 at 1602.437 kb on - strand, within MMP_RS08535 at 1602.438 kb on + strand, within MMP_RS08535 at 1602.438 kb on + strand, within MMP_RS08535 at 1602.438 kb on + strand, within MMP_RS08535 at 1602.504 kb on - strand, within MMP_RS08535 at 1602.567 kb on + strand, within MMP_RS08535 at 1602.582 kb on - strand, within MMP_RS08535 at 1602.769 kb on - strand at 1602.903 kb on - strand at 1602.917 kb on - strand at 1602.918 kb on + strand at 1602.928 kb on - strand at 1602.929 kb on + strand at 1603.073 kb on + strand at 1603.230 kb on - strand at 1603.352 kb on - strand at 1603.355 kb on + strand at 1603.356 kb on - strand at 1603.356 kb on - strand at 1603.357 kb on + strand at 1603.386 kb on - strand at 1603.391 kb on - strand at 1603.392 kb on + strand at 1603.392 kb on + strand at 1603.408 kb on + strand at 1603.665 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mc formate, casamino free, with 10mM L-Threonine remove 1,600,808 - +3.0 1,600,813 + +1.7 1,600,824 - +1.6 1,601,630 - +0.6 1,601,633 - +1.7 1,601,634 + -0.5 1,601,650 - +0.8 1,601,651 + -0.6 1,601,655 - -1.4 1,601,656 + -0.5 1,601,658 - +0.5 1,601,667 + -0.2 1,601,671 - -0.1 1,601,672 + -1.0 1,601,672 + -2.0 1,601,675 + +0.4 1,602,002 - MMP_RS08535 0.27 -0.8 1,602,003 + MMP_RS08535 0.27 +0.9 1,602,009 + MMP_RS08535 0.27 -1.7 1,602,009 + MMP_RS08535 0.27 +1.0 1,602,303 + MMP_RS08535 0.52 +0.3 1,602,353 - MMP_RS08535 0.56 -1.2 1,602,354 + MMP_RS08535 0.57 -1.5 1,602,354 + MMP_RS08535 0.57 -0.4 1,602,436 + MMP_RS08535 0.64 -0.9 1,602,436 + MMP_RS08535 0.64 -0.8 1,602,437 - MMP_RS08535 0.64 -1.1 1,602,438 + MMP_RS08535 0.64 -0.8 1,602,438 + MMP_RS08535 0.64 -2.7 1,602,438 + MMP_RS08535 0.64 +0.7 1,602,504 - MMP_RS08535 0.69 -1.1 1,602,567 + MMP_RS08535 0.75 -1.6 1,602,582 - MMP_RS08535 0.76 -1.6 1,602,769 - -0.4 1,602,903 - -0.1 1,602,917 - -0.1 1,602,918 + -1.3 1,602,928 - +0.4 1,602,929 + +0.6 1,603,073 + -2.2 1,603,230 - +0.7 1,603,352 - -1.6 1,603,355 + -1.1 1,603,356 - -2.0 1,603,356 - -1.5 1,603,357 + -1.2 1,603,386 - +0.3 1,603,391 - +1.3 1,603,392 + -3.7 1,603,392 + -1.5 1,603,408 + +1.0 1,603,665 + -2.8
Or see this region's nucleotide sequence