Strain Fitness in Methanococcus maripaludis S2 around MMP_RS08535

Experiment: Mc formate, casamino free, with 10mM L-Threonine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS08500 and MMP_RS08505 are separated by 114 nucleotidesMMP_RS08505 and MMP_RS08510 are separated by 29 nucleotidesMMP_RS08510 and MMP_RS08515 are separated by 13 nucleotidesMMP_RS08515 and MMP_RS08520 are separated by 14 nucleotidesMMP_RS08520 and MMP_RS08525 are separated by 38 nucleotidesMMP_RS08525 and MMP_RS08530 are separated by 116 nucleotidesMMP_RS08530 and MMP_RS08535 are separated by 122 nucleotidesMMP_RS08535 and MMP_RS08540 are separated by 52 nucleotidesMMP_RS08540 and MMP_RS08545 are separated by 159 nucleotides MMP_RS08500: MMP_RS08500 - pyridoxal 5'-phosphate synthase glutaminase subunit PdxT, at 1,600,220 to 1,600,783 _RS08500 MMP_RS08505: MMP_RS08505 - tRNA-His, at 1,600,898 to 1,600,972 _RS08505 MMP_RS08510: MMP_RS08510 - tRNA-Leu, at 1,601,002 to 1,601,086 _RS08510 MMP_RS08515: MMP_RS08515 - tRNA-Glu, at 1,601,100 to 1,601,174 _RS08515 MMP_RS08520: MMP_RS08520 - tRNA-Ile, at 1,601,189 to 1,601,263 _RS08520 MMP_RS08525: MMP_RS08525 - tRNA-Asn, at 1,601,302 to 1,601,378 _RS08525 MMP_RS08530: MMP_RS08530 - tRNA-Phe, at 1,601,495 to 1,601,568 _RS08530 MMP_RS08535: MMP_RS08535 - bis-aminopropyl spermidine synthase family protein, at 1,601,691 to 1,602,863 _RS08535 MMP_RS08540: MMP_RS08540 - 30S ribosomal protein S8e, at 1,602,916 to 1,603,302 _RS08540 MMP_RS08545: MMP_RS08545 - aspartate carbamoyltransferase, at 1,603,462 to 1,604,370 _RS08545 Position (kb) 1601 1602 1603Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1600.808 kb on - strandat 1600.813 kb on + strandat 1600.824 kb on - strandat 1601.630 kb on - strandat 1601.633 kb on - strandat 1601.634 kb on + strandat 1601.650 kb on - strandat 1601.651 kb on + strandat 1601.655 kb on - strandat 1601.656 kb on + strandat 1601.658 kb on - strandat 1601.667 kb on + strandat 1601.671 kb on - strandat 1601.672 kb on + strandat 1601.672 kb on + strandat 1601.675 kb on + strandat 1602.002 kb on - strand, within MMP_RS08535at 1602.003 kb on + strand, within MMP_RS08535at 1602.009 kb on + strand, within MMP_RS08535at 1602.009 kb on + strand, within MMP_RS08535at 1602.303 kb on + strand, within MMP_RS08535at 1602.353 kb on - strand, within MMP_RS08535at 1602.354 kb on + strand, within MMP_RS08535at 1602.354 kb on + strand, within MMP_RS08535at 1602.436 kb on + strand, within MMP_RS08535at 1602.436 kb on + strand, within MMP_RS08535at 1602.437 kb on - strand, within MMP_RS08535at 1602.438 kb on + strand, within MMP_RS08535at 1602.438 kb on + strand, within MMP_RS08535at 1602.438 kb on + strand, within MMP_RS08535at 1602.504 kb on - strand, within MMP_RS08535at 1602.567 kb on + strand, within MMP_RS08535at 1602.582 kb on - strand, within MMP_RS08535at 1602.769 kb on - strandat 1602.903 kb on - strandat 1602.917 kb on - strandat 1602.918 kb on + strandat 1602.928 kb on - strandat 1602.929 kb on + strandat 1603.073 kb on + strandat 1603.230 kb on - strandat 1603.352 kb on - strandat 1603.355 kb on + strandat 1603.356 kb on - strandat 1603.356 kb on - strandat 1603.357 kb on + strandat 1603.386 kb on - strandat 1603.391 kb on - strandat 1603.392 kb on + strandat 1603.392 kb on + strandat 1603.408 kb on + strandat 1603.665 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Mc formate, casamino free, with 10mM L-Threonine
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1,600,808 - +3.0
1,600,813 + +1.7
1,600,824 - +1.6
1,601,630 - +0.6
1,601,633 - +1.7
1,601,634 + -0.5
1,601,650 - +0.8
1,601,651 + -0.6
1,601,655 - -1.4
1,601,656 + -0.5
1,601,658 - +0.5
1,601,667 + -0.2
1,601,671 - -0.1
1,601,672 + -1.0
1,601,672 + -2.0
1,601,675 + +0.4
1,602,002 - MMP_RS08535 0.27 -0.8
1,602,003 + MMP_RS08535 0.27 +0.9
1,602,009 + MMP_RS08535 0.27 -1.7
1,602,009 + MMP_RS08535 0.27 +1.0
1,602,303 + MMP_RS08535 0.52 +0.3
1,602,353 - MMP_RS08535 0.56 -1.2
1,602,354 + MMP_RS08535 0.57 -1.5
1,602,354 + MMP_RS08535 0.57 -0.4
1,602,436 + MMP_RS08535 0.64 -0.9
1,602,436 + MMP_RS08535 0.64 -0.8
1,602,437 - MMP_RS08535 0.64 -1.1
1,602,438 + MMP_RS08535 0.64 -0.8
1,602,438 + MMP_RS08535 0.64 -2.7
1,602,438 + MMP_RS08535 0.64 +0.7
1,602,504 - MMP_RS08535 0.69 -1.1
1,602,567 + MMP_RS08535 0.75 -1.6
1,602,582 - MMP_RS08535 0.76 -1.6
1,602,769 - -0.4
1,602,903 - -0.1
1,602,917 - -0.1
1,602,918 + -1.3
1,602,928 - +0.4
1,602,929 + +0.6
1,603,073 + -2.2
1,603,230 - +0.7
1,603,352 - -1.6
1,603,355 + -1.1
1,603,356 - -2.0
1,603,356 - -1.5
1,603,357 + -1.2
1,603,386 - +0.3
1,603,391 - +1.3
1,603,392 + -3.7
1,603,392 + -1.5
1,603,408 + +1.0
1,603,665 + -2.8

Or see this region's nucleotide sequence