Experiment: Mc formate, casamino free, with 10mM L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07330 and MMP_RS07335 are separated by 112 nucleotides MMP_RS07335 and MMP_RS07340 are separated by 23 nucleotides MMP_RS07340 and MMP_RS07345 are separated by 3 nucleotides
MMP_RS07330: MMP_RS07330 - membrane protein, at 1,395,606 to 1,398,164
_RS07330
MMP_RS07335: MMP_RS07335 - tRNA (cytidine(56)-2'-O)-methyltransferase, at 1,398,277 to 1,398,816
_RS07335
MMP_RS07340: MMP_RS07340 - dCTP deaminase, at 1,398,840 to 1,399,460
_RS07340
MMP_RS07345: MMP_RS07345 - glycine synthesis protein GlyXL <small>(from data)</small>, at 1,399,464 to 1,400,840
_RS07345
Position (kb) 1398
1399 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 1397.375 kb on - strand, within MMP_RS07330 at 1397.391 kb on - strand, within MMP_RS07330 at 1397.391 kb on - strand, within MMP_RS07330 at 1397.411 kb on + strand, within MMP_RS07330 at 1397.439 kb on + strand, within MMP_RS07330 at 1397.439 kb on + strand, within MMP_RS07330 at 1397.444 kb on + strand, within MMP_RS07330 at 1397.462 kb on + strand, within MMP_RS07330 at 1397.493 kb on + strand, within MMP_RS07330 at 1397.497 kb on - strand, within MMP_RS07330 at 1397.589 kb on - strand, within MMP_RS07330 at 1397.590 kb on + strand, within MMP_RS07330 at 1397.590 kb on + strand, within MMP_RS07330 at 1397.606 kb on - strand, within MMP_RS07330 at 1397.646 kb on + strand, within MMP_RS07330 at 1397.733 kb on + strand, within MMP_RS07330 at 1397.742 kb on - strand, within MMP_RS07330 at 1397.743 kb on + strand, within MMP_RS07330 at 1397.747 kb on + strand, within MMP_RS07330 at 1397.753 kb on + strand, within MMP_RS07330 at 1397.769 kb on - strand, within MMP_RS07330 at 1398.020 kb on - strand at 1398.021 kb on + strand at 1398.136 kb on - strand at 1398.163 kb on + strand at 1398.206 kb on + strand at 1398.291 kb on + strand at 1398.333 kb on - strand, within MMP_RS07335 at 1398.348 kb on + strand, within MMP_RS07335 at 1398.512 kb on - strand, within MMP_RS07335 at 1398.513 kb on + strand, within MMP_RS07335 at 1398.515 kb on - strand, within MMP_RS07335 at 1398.516 kb on + strand, within MMP_RS07335 at 1398.523 kb on + strand, within MMP_RS07335 at 1398.652 kb on - strand, within MMP_RS07335 at 1398.838 kb on + strand at 1398.838 kb on + strand at 1398.847 kb on - strand at 1398.871 kb on + strand at 1399.001 kb on + strand at 1399.525 kb on - strand at 1399.526 kb on + strand at 1399.526 kb on + strand at 1399.532 kb on - strand at 1399.533 kb on + strand at 1399.663 kb on + strand, within MMP_RS07345 at 1399.679 kb on + strand, within MMP_RS07345
Per-strain Table
Position Strand Gene LocusTag Fraction Mc formate, casamino free, with 10mM L-Threonine remove 1,397,375 - MMP_RS07330 0.69 -1.0 1,397,391 - MMP_RS07330 0.70 +2.3 1,397,391 - MMP_RS07330 0.70 +0.2 1,397,411 + MMP_RS07330 0.71 -2.1 1,397,439 + MMP_RS07330 0.72 +2.9 1,397,439 + MMP_RS07330 0.72 -0.4 1,397,444 + MMP_RS07330 0.72 -0.2 1,397,462 + MMP_RS07330 0.73 +1.9 1,397,493 + MMP_RS07330 0.74 +1.5 1,397,497 - MMP_RS07330 0.74 +0.9 1,397,589 - MMP_RS07330 0.77 +0.2 1,397,590 + MMP_RS07330 0.78 -0.1 1,397,590 + MMP_RS07330 0.78 +1.9 1,397,606 - MMP_RS07330 0.78 -0.0 1,397,646 + MMP_RS07330 0.80 +0.9 1,397,733 + MMP_RS07330 0.83 +0.5 1,397,742 - MMP_RS07330 0.83 +2.9 1,397,743 + MMP_RS07330 0.84 +0.5 1,397,747 + MMP_RS07330 0.84 -0.2 1,397,753 + MMP_RS07330 0.84 -1.1 1,397,769 - MMP_RS07330 0.85 +0.3 1,398,020 - -0.6 1,398,021 + +0.2 1,398,136 - +2.1 1,398,163 + +1.7 1,398,206 + -3.1 1,398,291 + -4.7 1,398,333 - MMP_RS07335 0.10 -2.4 1,398,348 + MMP_RS07335 0.13 -1.7 1,398,512 - MMP_RS07335 0.44 -3.2 1,398,513 + MMP_RS07335 0.44 -1.9 1,398,515 - MMP_RS07335 0.44 -0.7 1,398,516 + MMP_RS07335 0.44 -2.2 1,398,523 + MMP_RS07335 0.46 -3.5 1,398,652 - MMP_RS07335 0.69 -0.3 1,398,838 + -1.9 1,398,838 + -1.5 1,398,847 - -2.6 1,398,871 + -1.8 1,399,001 + -2.8 1,399,525 - -3.5 1,399,526 + -3.6 1,399,526 + -2.9 1,399,532 - -3.2 1,399,533 + -3.8 1,399,663 + MMP_RS07345 0.14 -1.8 1,399,679 + MMP_RS07345 0.16 -1.7
Or see this region's nucleotide sequence