Experiment: Mc formate, casamino free, with 10mM L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04665 and MMP_RS04670 are separated by 301 nucleotides MMP_RS04670 and MMP_RS04675 are separated by 9 nucleotides MMP_RS04675 and MMP_RS04680 are separated by 29 nucleotides
MMP_RS04665: MMP_RS04665 - SBBP repeat-containing protein, at 891,545 to 894,625
_RS04665
MMP_RS04670: MMP_RS04670 - selenouridine synthase SelU-like subunit, at 894,927 to 895,586
_RS04670
MMP_RS04675: MMP_RS04675 - selenouridine synthase SelU-like subunit, at 895,596 to 896,249
_RS04675
MMP_RS04680: MMP_RS04680 - NOG1 family protein, at 896,279 to 897,316
_RS04680
Position (kb) 894
895
896 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 893.965 kb on + strand, within MMP_RS04665 at 893.992 kb on + strand, within MMP_RS04665 at 893.992 kb on + strand, within MMP_RS04665 at 894.025 kb on - strand, within MMP_RS04665 at 894.026 kb on + strand, within MMP_RS04665 at 894.026 kb on + strand, within MMP_RS04665 at 894.030 kb on - strand, within MMP_RS04665 at 894.030 kb on - strand, within MMP_RS04665 at 894.030 kb on - strand, within MMP_RS04665 at 894.030 kb on - strand, within MMP_RS04665 at 894.031 kb on + strand, within MMP_RS04665 at 894.048 kb on - strand, within MMP_RS04665 at 894.049 kb on + strand, within MMP_RS04665 at 894.049 kb on + strand, within MMP_RS04665 at 894.061 kb on - strand, within MMP_RS04665 at 894.068 kb on - strand, within MMP_RS04665 at 894.069 kb on + strand, within MMP_RS04665 at 894.069 kb on + strand, within MMP_RS04665 at 894.104 kb on + strand, within MMP_RS04665 at 894.130 kb on - strand, within MMP_RS04665 at 894.147 kb on - strand, within MMP_RS04665 at 894.152 kb on - strand, within MMP_RS04665 at 894.179 kb on + strand, within MMP_RS04665 at 894.179 kb on + strand, within MMP_RS04665 at 894.193 kb on - strand, within MMP_RS04665 at 894.194 kb on + strand, within MMP_RS04665 at 894.196 kb on + strand, within MMP_RS04665 at 894.207 kb on - strand, within MMP_RS04665 at 894.221 kb on - strand, within MMP_RS04665 at 894.252 kb on - strand, within MMP_RS04665 at 894.303 kb on - strand, within MMP_RS04665 at 894.345 kb on - strand at 894.346 kb on + strand at 894.445 kb on + strand at 894.445 kb on + strand at 894.465 kb on - strand at 894.465 kb on - strand at 894.465 kb on - strand at 894.466 kb on + strand at 894.466 kb on + strand at 894.470 kb on + strand at 894.470 kb on + strand at 894.470 kb on + strand at 894.483 kb on - strand at 894.484 kb on + strand at 894.538 kb on - strand at 894.539 kb on + strand at 894.649 kb on - strand at 894.649 kb on - strand at 894.649 kb on - strand at 894.649 kb on - strand at 894.650 kb on + strand at 894.665 kb on + strand at 894.665 kb on + strand at 894.666 kb on - strand at 894.680 kb on - strand at 894.681 kb on + strand at 894.727 kb on + strand at 894.757 kb on - strand at 894.757 kb on - strand at 894.758 kb on + strand at 894.762 kb on + strand at 894.804 kb on - strand at 894.805 kb on + strand at 894.809 kb on - strand at 894.810 kb on + strand at 894.892 kb on - strand at 894.903 kb on - strand at 894.906 kb on - strand at 894.911 kb on - strand at 894.911 kb on - strand at 894.922 kb on - strand at 894.988 kb on - strand at 895.000 kb on + strand, within MMP_RS04670 at 895.002 kb on + strand, within MMP_RS04670 at 895.006 kb on - strand, within MMP_RS04670 at 895.007 kb on + strand, within MMP_RS04670 at 895.007 kb on + strand, within MMP_RS04670 at 895.060 kb on + strand, within MMP_RS04670 at 895.067 kb on + strand, within MMP_RS04670 at 895.067 kb on + strand, within MMP_RS04670 at 895.094 kb on - strand, within MMP_RS04670 at 895.130 kb on - strand, within MMP_RS04670 at 895.186 kb on - strand, within MMP_RS04670 at 895.232 kb on - strand, within MMP_RS04670 at 895.233 kb on + strand, within MMP_RS04670 at 895.233 kb on + strand, within MMP_RS04670 at 895.235 kb on + strand, within MMP_RS04670 at 895.417 kb on + strand, within MMP_RS04670 at 895.546 kb on + strand at 895.578 kb on + strand at 895.625 kb on - strand at 895.644 kb on + strand at 895.649 kb on + strand at 895.679 kb on + strand, within MMP_RS04675 at 895.685 kb on + strand, within MMP_RS04675 at 895.757 kb on - strand, within MMP_RS04675 at 895.761 kb on + strand, within MMP_RS04675 at 895.777 kb on - strand, within MMP_RS04675 at 895.817 kb on - strand, within MMP_RS04675 at 895.827 kb on - strand, within MMP_RS04675 at 895.869 kb on + strand, within MMP_RS04675 at 895.906 kb on - strand, within MMP_RS04675 at 895.907 kb on + strand, within MMP_RS04675 at 895.909 kb on - strand, within MMP_RS04675 at 895.995 kb on - strand, within MMP_RS04675 at 896.017 kb on + strand, within MMP_RS04675 at 896.062 kb on - strand, within MMP_RS04675 at 896.062 kb on - strand, within MMP_RS04675 at 896.212 kb on - strand at 896.229 kb on - strand at 896.231 kb on - strand at 896.234 kb on - strand at 896.234 kb on - strand at 896.234 kb on - strand at 896.248 kb on + strand at 896.288 kb on - strand at 896.288 kb on - strand at 896.320 kb on - strand at 896.324 kb on - strand at 896.324 kb on - strand at 896.340 kb on + strand at 896.414 kb on + strand at 896.504 kb on + strand at 896.575 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mc formate, casamino free, with 10mM L-Threonine remove 893,965 + MMP_RS04665 0.79 -1.4 893,992 + MMP_RS04665 0.79 -0.2 893,992 + MMP_RS04665 0.79 +0.6 894,025 - MMP_RS04665 0.80 +1.0 894,026 + MMP_RS04665 0.81 +0.8 894,026 + MMP_RS04665 0.81 -1.0 894,030 - MMP_RS04665 0.81 +0.0 894,030 - MMP_RS04665 0.81 +0.2 894,030 - MMP_RS04665 0.81 -0.6 894,030 - MMP_RS04665 0.81 +1.4 894,031 + MMP_RS04665 0.81 -2.2 894,048 - MMP_RS04665 0.81 +1.5 894,049 + MMP_RS04665 0.81 -0.2 894,049 + MMP_RS04665 0.81 -1.0 894,061 - MMP_RS04665 0.82 +0.4 894,068 - MMP_RS04665 0.82 +0.3 894,069 + MMP_RS04665 0.82 +0.2 894,069 + MMP_RS04665 0.82 +0.6 894,104 + MMP_RS04665 0.83 -0.2 894,130 - MMP_RS04665 0.84 -0.1 894,147 - MMP_RS04665 0.84 +1.0 894,152 - MMP_RS04665 0.85 +1.8 894,179 + MMP_RS04665 0.85 -0.1 894,179 + MMP_RS04665 0.85 +1.6 894,193 - MMP_RS04665 0.86 -0.4 894,194 + MMP_RS04665 0.86 +0.1 894,196 + MMP_RS04665 0.86 +0.4 894,207 - MMP_RS04665 0.86 +0.1 894,221 - MMP_RS04665 0.87 -0.1 894,252 - MMP_RS04665 0.88 -0.7 894,303 - MMP_RS04665 0.90 +1.2 894,345 - -0.3 894,346 + +1.3 894,445 + -0.7 894,445 + +0.3 894,465 - -1.8 894,465 - -0.9 894,465 - -0.6 894,466 + -0.9 894,466 + -1.1 894,470 + +1.2 894,470 + +0.5 894,470 + -2.1 894,483 - +0.9 894,484 + +1.4 894,538 - +0.0 894,539 + +1.2 894,649 - +0.7 894,649 - -0.3 894,649 - +0.9 894,649 - +0.9 894,650 + -0.8 894,665 + +1.7 894,665 + +0.2 894,666 - +1.2 894,680 - +1.5 894,681 + +0.1 894,727 + -1.1 894,757 - -1.3 894,757 - -0.4 894,758 + +0.8 894,762 + +1.0 894,804 - +0.8 894,805 + +0.9 894,809 - +0.0 894,810 + +1.3 894,892 - -0.5 894,903 - -1.1 894,906 - -1.4 894,911 - +0.1 894,911 - -0.2 894,922 - -0.9 894,988 - -0.2 895,000 + MMP_RS04670 0.11 -0.6 895,002 + MMP_RS04670 0.11 -0.7 895,006 - MMP_RS04670 0.12 +0.4 895,007 + MMP_RS04670 0.12 -0.9 895,007 + MMP_RS04670 0.12 -0.3 895,060 + MMP_RS04670 0.20 -1.1 895,067 + MMP_RS04670 0.21 -1.0 895,067 + MMP_RS04670 0.21 -1.4 895,094 - MMP_RS04670 0.25 -0.1 895,130 - MMP_RS04670 0.31 -3.5 895,186 - MMP_RS04670 0.39 -1.8 895,232 - MMP_RS04670 0.46 -0.1 895,233 + MMP_RS04670 0.46 -0.6 895,233 + MMP_RS04670 0.46 -0.1 895,235 + MMP_RS04670 0.47 -2.3 895,417 + MMP_RS04670 0.74 -0.9 895,546 + -1.7 895,578 + -1.7 895,625 - -0.4 895,644 + +0.4 895,649 + -1.1 895,679 + MMP_RS04675 0.13 -1.2 895,685 + MMP_RS04675 0.14 -0.2 895,757 - MMP_RS04675 0.25 -1.3 895,761 + MMP_RS04675 0.25 -1.4 895,777 - MMP_RS04675 0.28 +1.1 895,817 - MMP_RS04675 0.34 -1.1 895,827 - MMP_RS04675 0.35 +0.3 895,869 + MMP_RS04675 0.42 +0.5 895,906 - MMP_RS04675 0.47 -2.1 895,907 + MMP_RS04675 0.48 -0.3 895,909 - MMP_RS04675 0.48 -0.8 895,995 - MMP_RS04675 0.61 -0.8 896,017 + MMP_RS04675 0.64 -0.6 896,062 - MMP_RS04675 0.71 -0.4 896,062 - MMP_RS04675 0.71 -0.2 896,212 - -0.2 896,229 - +1.0 896,231 - +0.8 896,234 - +0.8 896,234 - +0.4 896,234 - -0.1 896,248 + -2.0 896,288 - -0.0 896,288 - -0.5 896,320 - +0.5 896,324 - +0.6 896,324 - -0.3 896,340 + +0.0 896,414 + -1.1 896,504 + +1.9 896,575 + +0.8
Or see this region's nucleotide sequence