Experiment: Mc formate, casamino free, with 10mM L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00755 and MMP_RS00760 are separated by 49 nucleotides MMP_RS00760 and MMP_RS00765 are separated by 72 nucleotides MMP_RS00765 and MMP_RS00770 are separated by 49 nucleotides MMP_RS00770 and MMP_RS00775 are separated by 270 nucleotides
MMP_RS00755: MMP_RS00755 - tyrosine decarboxylase MfnA, at 137,305 to 138,459
_RS00755
MMP_RS00760: MMP_RS00760 - branched-chain-amino-acid transaminase, at 138,509 to 139,372
_RS00760
MMP_RS00765: MMP_RS00765 - IMP dehydrogenase, at 139,445 to 140,947
_RS00765
MMP_RS00770: MMP_RS00770 - hypothetical protein, at 140,997 to 141,341
_RS00770
MMP_RS00775: MMP_RS00775 - threonine synthase, at 141,612 to 142,826
_RS00775
Position (kb)
139
140
141 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 138.458 kb on + strand at 138.464 kb on - strand at 138.564 kb on - strand at 138.660 kb on - strand, within MMP_RS00760 at 138.753 kb on - strand, within MMP_RS00760 at 138.753 kb on - strand, within MMP_RS00760 at 138.754 kb on + strand, within MMP_RS00760 at 138.764 kb on - strand, within MMP_RS00760 at 138.765 kb on + strand, within MMP_RS00760 at 138.768 kb on - strand, within MMP_RS00760 at 138.769 kb on + strand, within MMP_RS00760 at 138.769 kb on + strand, within MMP_RS00760 at 138.895 kb on + strand, within MMP_RS00760 at 139.062 kb on - strand, within MMP_RS00760 at 139.072 kb on - strand, within MMP_RS00760 at 139.081 kb on - strand, within MMP_RS00760 at 139.082 kb on + strand, within MMP_RS00760 at 139.371 kb on + strand at 139.433 kb on - strand at 139.434 kb on + strand at 139.446 kb on - strand at 139.625 kb on - strand at 140.291 kb on + strand at 140.457 kb on + strand at 140.898 kb on - strand at 140.927 kb on - strand at 140.977 kb on - strand at 140.977 kb on - strand at 140.981 kb on - strand at 140.982 kb on + strand at 141.194 kb on - strand, within MMP_RS00770 at 141.195 kb on + strand, within MMP_RS00770 at 141.226 kb on - strand, within MMP_RS00770 at 141.319 kb on - strand at 141.340 kb on + strand at 141.396 kb on - strand at 141.400 kb on - strand at 141.426 kb on - strand at 141.427 kb on + strand at 141.428 kb on - strand at 141.429 kb on + strand at 141.429 kb on + strand at 141.429 kb on + strand at 141.429 kb on + strand at 141.430 kb on - strand at 141.431 kb on + strand at 141.431 kb on + strand at 141.431 kb on + strand at 141.431 kb on + strand at 141.459 kb on - strand at 141.460 kb on + strand at 141.485 kb on + strand at 141.531 kb on + strand at 141.557 kb on + strand at 141.562 kb on - strand at 141.611 kb on - strand at 141.612 kb on + strand at 141.631 kb on - strand at 141.787 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mc formate, casamino free, with 10mM L-Threonine remove 138,458 + +0.5 138,464 - +0.3 138,564 - +0.6 138,660 - MMP_RS00760 0.17 -0.2 138,753 - MMP_RS00760 0.28 -1.1 138,753 - MMP_RS00760 0.28 +0.7 138,754 + MMP_RS00760 0.28 -0.7 138,764 - MMP_RS00760 0.30 -1.6 138,765 + MMP_RS00760 0.30 -0.9 138,768 - MMP_RS00760 0.30 -1.7 138,769 + MMP_RS00760 0.30 +0.1 138,769 + MMP_RS00760 0.30 +0.5 138,895 + MMP_RS00760 0.45 +0.3 139,062 - MMP_RS00760 0.64 -1.7 139,072 - MMP_RS00760 0.65 -1.6 139,081 - MMP_RS00760 0.66 -0.5 139,082 + MMP_RS00760 0.66 +0.4 139,371 + -0.4 139,433 - +3.9 139,434 + +1.3 139,446 - +3.2 139,625 - -1.9 140,291 + +1.1 140,457 + +0.5 140,898 - +1.7 140,927 - +0.2 140,977 - +3.1 140,977 - +0.4 140,981 - +4.3 140,982 + +4.9 141,194 - MMP_RS00770 0.57 +1.9 141,195 + MMP_RS00770 0.57 +1.2 141,226 - MMP_RS00770 0.66 +0.4 141,319 - +0.5 141,340 + -1.1 141,396 - +0.1 141,400 - -0.8 141,426 - +1.3 141,427 + -1.4 141,428 - +1.5 141,429 + -0.4 141,429 + +0.6 141,429 + -1.1 141,429 + +0.7 141,430 - +0.6 141,431 + +0.1 141,431 + -0.1 141,431 + +0.3 141,431 + +0.7 141,459 - -0.3 141,460 + +0.6 141,485 + -0.6 141,531 + +0.1 141,557 + +1.3 141,562 - +0.7 141,611 - -2.5 141,612 + -0.5 141,631 - -0.8 141,787 - +0.4
Or see this region's nucleotide sequence