Experiment: L-Aspartic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5705 and Pf6N2E2_5706 are separated by 163 nucleotides Pf6N2E2_5706 and Pf6N2E2_5707 are separated by 333 nucleotides
Pf6N2E2_5705 - Sensor protein PhoQ (EC 2.7.13.3), at 6,520,389 to 6,521,735
_5705
Pf6N2E2_5706 - Transcriptional regulator, AraC family, at 6,521,899 to 6,522,897
_5706
Pf6N2E2_5707 - Aerobic C4-dicarboxylate transporter for fumarate, L-malate, D-malate, succunate, at 6,523,231 to 6,524,583
_5707
Position (kb)
6521
6522
6523 Strain fitness (log2 ratio)
-2
-1
0
1 at 6521.661 kb on + strand at 6521.742 kb on + strand at 6522.035 kb on + strand, within Pf6N2E2_5706 at 6522.035 kb on + strand, within Pf6N2E2_5706 at 6522.035 kb on + strand, within Pf6N2E2_5706 at 6522.035 kb on + strand, within Pf6N2E2_5706 at 6522.036 kb on - strand, within Pf6N2E2_5706 at 6522.211 kb on - strand, within Pf6N2E2_5706 at 6522.469 kb on + strand, within Pf6N2E2_5706 at 6522.590 kb on + strand, within Pf6N2E2_5706 at 6522.591 kb on - strand, within Pf6N2E2_5706 at 6522.591 kb on - strand, within Pf6N2E2_5706 at 6522.676 kb on - strand, within Pf6N2E2_5706 at 6522.701 kb on + strand, within Pf6N2E2_5706 at 6522.701 kb on + strand, within Pf6N2E2_5706 at 6522.702 kb on - strand, within Pf6N2E2_5706 at 6522.824 kb on + strand at 6522.825 kb on - strand at 6522.825 kb on - strand at 6522.825 kb on - strand at 6522.930 kb on - strand at 6522.981 kb on + strand at 6522.981 kb on + strand at 6522.981 kb on + strand at 6523.068 kb on + strand at 6523.068 kb on + strand at 6523.103 kb on + strand at 6523.264 kb on + strand at 6523.475 kb on - strand, within Pf6N2E2_5707 at 6523.475 kb on - strand, within Pf6N2E2_5707 at 6523.475 kb on - strand, within Pf6N2E2_5707 at 6523.545 kb on - strand, within Pf6N2E2_5707 at 6523.562 kb on + strand, within Pf6N2E2_5707 at 6523.562 kb on + strand, within Pf6N2E2_5707 at 6523.562 kb on + strand, within Pf6N2E2_5707 at 6523.562 kb on + strand, within Pf6N2E2_5707 at 6523.562 kb on + strand, within Pf6N2E2_5707 at 6523.744 kb on + strand, within Pf6N2E2_5707 at 6523.867 kb on + strand, within Pf6N2E2_5707 at 6523.868 kb on - strand, within Pf6N2E2_5707
Per-strain Table
Position Strand Gene LocusTag Fraction L-Aspartic Acid (N) remove 6,521,661 + -1.3 6,521,742 + +0.5 6,522,035 + Pf6N2E2_5706 0.14 -0.5 6,522,035 + Pf6N2E2_5706 0.14 +0.4 6,522,035 + Pf6N2E2_5706 0.14 -0.2 6,522,035 + Pf6N2E2_5706 0.14 -0.7 6,522,036 - Pf6N2E2_5706 0.14 -0.6 6,522,211 - Pf6N2E2_5706 0.31 -0.7 6,522,469 + Pf6N2E2_5706 0.57 -0.2 6,522,590 + Pf6N2E2_5706 0.69 +0.9 6,522,591 - Pf6N2E2_5706 0.69 +1.5 6,522,591 - Pf6N2E2_5706 0.69 -1.4 6,522,676 - Pf6N2E2_5706 0.78 -1.9 6,522,701 + Pf6N2E2_5706 0.80 -0.1 6,522,701 + Pf6N2E2_5706 0.80 -1.7 6,522,702 - Pf6N2E2_5706 0.80 -1.5 6,522,824 + -0.1 6,522,825 - -0.7 6,522,825 - -1.9 6,522,825 - +0.8 6,522,930 - -1.2 6,522,981 + +1.1 6,522,981 + +0.7 6,522,981 + +1.1 6,523,068 + +1.3 6,523,068 + +0.5 6,523,103 + -0.5 6,523,264 + +0.3 6,523,475 - Pf6N2E2_5707 0.18 +1.0 6,523,475 - Pf6N2E2_5707 0.18 +0.3 6,523,475 - Pf6N2E2_5707 0.18 +0.5 6,523,545 - Pf6N2E2_5707 0.23 +1.1 6,523,562 + Pf6N2E2_5707 0.24 -0.8 6,523,562 + Pf6N2E2_5707 0.24 +0.6 6,523,562 + Pf6N2E2_5707 0.24 -1.7 6,523,562 + Pf6N2E2_5707 0.24 -0.7 6,523,562 + Pf6N2E2_5707 0.24 +1.7 6,523,744 + Pf6N2E2_5707 0.38 -1.0 6,523,867 + Pf6N2E2_5707 0.47 -0.3 6,523,868 - Pf6N2E2_5707 0.47 +0.7
Or see this region's nucleotide sequence