Experiment: L-Aspartic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_519 and Pf6N2E2_520 are separated by 10 nucleotides Pf6N2E2_520 and Pf6N2E2_521 are separated by 15 nucleotides Pf6N2E2_521 and Pf6N2E2_522 are separated by 51 nucleotides
Pf6N2E2_519 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 589,541 to 590,593
_519
Pf6N2E2_520 - hypothetical protein, at 590,604 to 590,720
_520
Pf6N2E2_521 - hypothetical protein, at 590,736 to 590,888
_521
Pf6N2E2_522 - Inositol transport system sugar-binding protein, at 590,940 to 591,866
_522
Position (kb) 590
591 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 589.998 kb on - strand, within Pf6N2E2_519 at 590.121 kb on - strand, within Pf6N2E2_519 at 590.261 kb on + strand, within Pf6N2E2_519 at 590.262 kb on - strand, within Pf6N2E2_519 at 590.262 kb on - strand, within Pf6N2E2_519 at 590.262 kb on - strand, within Pf6N2E2_519 at 590.429 kb on + strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.724 kb on + strand at 590.724 kb on + strand at 590.724 kb on + strand at 590.724 kb on + strand at 590.725 kb on - strand at 590.729 kb on - strand at 590.763 kb on + strand, within Pf6N2E2_521 at 590.763 kb on + strand, within Pf6N2E2_521 at 590.763 kb on + strand, within Pf6N2E2_521 at 590.763 kb on + strand, within Pf6N2E2_521 at 590.764 kb on - strand, within Pf6N2E2_521 at 590.764 kb on - strand, within Pf6N2E2_521 at 590.907 kb on + strand at 591.060 kb on + strand, within Pf6N2E2_522 at 591.061 kb on - strand, within Pf6N2E2_522 at 591.061 kb on - strand, within Pf6N2E2_522 at 591.478 kb on - strand, within Pf6N2E2_522 at 591.658 kb on + strand, within Pf6N2E2_522 at 591.770 kb on + strand, within Pf6N2E2_522 at 591.770 kb on + strand, within Pf6N2E2_522
Per-strain Table
Position Strand Gene LocusTag Fraction L-Aspartic Acid (N) remove 589,998 - Pf6N2E2_519 0.43 -0.3 590,121 - Pf6N2E2_519 0.55 +0.0 590,261 + Pf6N2E2_519 0.68 +0.7 590,262 - Pf6N2E2_519 0.68 +0.4 590,262 - Pf6N2E2_519 0.68 -2.8 590,262 - Pf6N2E2_519 0.68 -0.7 590,429 + Pf6N2E2_519 0.84 +1.0 590,430 - Pf6N2E2_519 0.84 -0.3 590,430 - Pf6N2E2_519 0.84 +0.2 590,430 - Pf6N2E2_519 0.84 +0.4 590,430 - Pf6N2E2_519 0.84 -1.8 590,430 - Pf6N2E2_519 0.84 +0.2 590,430 - Pf6N2E2_519 0.84 -0.8 590,724 + -0.3 590,724 + -0.1 590,724 + +1.0 590,724 + +0.3 590,725 - +0.5 590,729 - +0.3 590,763 + Pf6N2E2_521 0.18 +0.6 590,763 + Pf6N2E2_521 0.18 +0.4 590,763 + Pf6N2E2_521 0.18 +0.1 590,763 + Pf6N2E2_521 0.18 +2.2 590,764 - Pf6N2E2_521 0.18 -1.2 590,764 - Pf6N2E2_521 0.18 +0.0 590,907 + -0.9 591,060 + Pf6N2E2_522 0.13 +1.1 591,061 - Pf6N2E2_522 0.13 +1.4 591,061 - Pf6N2E2_522 0.13 +0.1 591,478 - Pf6N2E2_522 0.58 +1.6 591,658 + Pf6N2E2_522 0.77 -0.5 591,770 + Pf6N2E2_522 0.90 +0.4 591,770 + Pf6N2E2_522 0.90 +1.1
Or see this region's nucleotide sequence