Experiment: L-Aspartic Acid (N) 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf6N2E2_4761 and Pf6N2E2_4762 overlap by 8 nucleotides Pf6N2E2_4762 and Pf6N2E2_4763 overlap by 8 nucleotides Pf6N2E2_4763 and Pf6N2E2_4764 overlap by 4 nucleotides 
        Pf6N2E2_4761 - 8-amino-7-oxononanoate synthase (EC 2.3.1.47), at 5,499,374 to 5,500,552 
        _4761 
         
        
        Pf6N2E2_4762 - Biotin synthesis protein BioH, at 5,500,545 to 5,501,276 
        _4762 
         
        
        Pf6N2E2_4763 - Biotin synthesis protein BioC, at 5,501,269 to 5,502,081 
        _4763 
         
        
        Pf6N2E2_4764 - Dethiobiotin synthetase (EC 6.3.3.3), at 5,502,078 to 5,502,758 
        _4764 
         Position (kb) 5500 
5501 
5502 Strain fitness (log2 ratio) -3 
-2 
-1 
0 
1 at 5499.692 kb on + strand, within Pf6N2E2_4761 at 5499.692 kb on + strand, within Pf6N2E2_4761 at 5499.692 kb on + strand, within Pf6N2E2_4761 at 5499.692 kb on + strand, within Pf6N2E2_4761 at 5499.693 kb on - strand, within Pf6N2E2_4761 at 5499.816 kb on - strand, within Pf6N2E2_4761 at 5499.872 kb on - strand, within Pf6N2E2_4761 at 5500.157 kb on + strand, within Pf6N2E2_4761 at 5500.157 kb on + strand, within Pf6N2E2_4761 at 5500.157 kb on + strand, within Pf6N2E2_4761 at 5500.157 kb on + strand, within Pf6N2E2_4761 at 5500.157 kb on + strand, within Pf6N2E2_4761 at 5500.158 kb on - strand, within Pf6N2E2_4761 at 5500.163 kb on + strand, within Pf6N2E2_4761 at 5500.163 kb on + strand, within Pf6N2E2_4761 at 5500.163 kb on + strand, within Pf6N2E2_4761 at 5500.163 kb on + strand, within Pf6N2E2_4761 at 5500.163 kb on + strand, within Pf6N2E2_4761 at 5500.163 kb on + strand, within Pf6N2E2_4761 at 5500.163 kb on + strand, within Pf6N2E2_4761 at 5500.164 kb on - strand, within Pf6N2E2_4761 at 5500.164 kb on - strand, within Pf6N2E2_4761 at 5500.164 kb on - strand, within Pf6N2E2_4761 at 5500.164 kb on - strand, within Pf6N2E2_4761 at 5500.164 kb on - strand, within Pf6N2E2_4761 at 5500.164 kb on - strand, within Pf6N2E2_4761 at 5500.164 kb on - strand, within Pf6N2E2_4761 at 5500.164 kb on - strand, within Pf6N2E2_4761 at 5500.164 kb on - strand, within Pf6N2E2_4761 at 5500.319 kb on - strand, within Pf6N2E2_4761 at 5500.319 kb on - strand, within Pf6N2E2_4761 at 5500.319 kb on - strand, within Pf6N2E2_4761 at 5500.491 kb on + strand at 5500.491 kb on + strand at 5500.491 kb on + strand at 5500.491 kb on + strand at 5500.491 kb on + strand at 5500.491 kb on + strand at 5500.491 kb on + strand at 5500.492 kb on - strand at 5500.492 kb on - strand at 5500.561 kb on + strand at 5500.561 kb on + strand at 5500.561 kb on + strand at 5500.561 kb on + strand at 5500.561 kb on + strand at 5500.561 kb on + strand at 5500.562 kb on - strand at 5500.562 kb on - strand at 5500.987 kb on + strand, within Pf6N2E2_4762 at 5500.987 kb on + strand, within Pf6N2E2_4762 at 5500.988 kb on - strand, within Pf6N2E2_4762 at 5501.057 kb on + strand, within Pf6N2E2_4762 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.357 kb on - strand, within Pf6N2E2_4763 at 5501.357 kb on - strand, within Pf6N2E2_4763 at 5501.357 kb on - strand, within Pf6N2E2_4763 at 5501.357 kb on - strand, within Pf6N2E2_4763 at 5501.464 kb on + strand, within Pf6N2E2_4763 at 5501.465 kb on - strand, within Pf6N2E2_4763 at 5501.465 kb on - strand, within Pf6N2E2_4763 at 5501.735 kb on + strand, within Pf6N2E2_4763 at 5501.735 kb on + strand, within Pf6N2E2_4763 at 5501.736 kb on - strand, within Pf6N2E2_4763 at 5501.818 kb on + strand, within Pf6N2E2_4763 at 5501.818 kb on + strand, within Pf6N2E2_4763 at 5501.818 kb on + strand, within Pf6N2E2_4763 at 5501.818 kb on + strand, within Pf6N2E2_4763 at 5501.818 kb on + strand, within Pf6N2E2_4763 at 5501.819 kb on - strand, within Pf6N2E2_4763 at 5501.819 kb on - strand, within Pf6N2E2_4763 at 5501.819 kb on - strand, within Pf6N2E2_4763 at 5501.819 kb on - strand, within Pf6N2E2_4763 at 5501.819 kb on - strand, within Pf6N2E2_4763 at 5501.888 kb on - strand, within Pf6N2E2_4763 at 5502.004 kb on + strand at 5502.004 kb on + strand at 5502.004 kb on + strand at 5502.004 kb on + strand at 5502.005 kb on - strand at 5502.005 kb on - strand at 5502.043 kb on + strand at 5502.043 kb on + strand at 5502.043 kb on + strand at 5502.043 kb on + strand at 5502.043 kb on + strand at 5502.044 kb on - strand at 5502.044 kb on - strand at 5502.078 kb on - strand at 5502.090 kb on + strand at 5502.091 kb on - strand  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction L-Aspartic Acid (N) remove 5,499,692 +  Pf6N2E2_4761 0.27  -2.8 5,499,692 +  Pf6N2E2_4761 0.27  -2.0 5,499,692 +  Pf6N2E2_4761 0.27  -2.9 5,499,692 +  Pf6N2E2_4761 0.27  -1.5 5,499,693 -  Pf6N2E2_4761 0.27  -1.7 5,499,816 -  Pf6N2E2_4761 0.37  -2.5 5,499,872 -  Pf6N2E2_4761 0.42  -1.4 5,500,157 +  Pf6N2E2_4761 0.66  -1.0 5,500,157 +  Pf6N2E2_4761 0.66  -2.5 5,500,157 +  Pf6N2E2_4761 0.66  -1.9 5,500,157 +  Pf6N2E2_4761 0.66  -2.7 5,500,157 +  Pf6N2E2_4761 0.66  -2.5 5,500,158 -  Pf6N2E2_4761 0.66  -0.6 5,500,163 +  Pf6N2E2_4761 0.67  -2.8 5,500,163 +  Pf6N2E2_4761 0.67  -0.3 5,500,163 +  Pf6N2E2_4761 0.67  +0.3 5,500,163 +  Pf6N2E2_4761 0.67  -0.7 5,500,163 +  Pf6N2E2_4761 0.67  -2.1 5,500,163 +  Pf6N2E2_4761 0.67  -1.2 5,500,163 +  Pf6N2E2_4761 0.67  -2.4 5,500,164 -  Pf6N2E2_4761 0.67  -1.8 5,500,164 -  Pf6N2E2_4761 0.67  -0.1 5,500,164 -  Pf6N2E2_4761 0.67  -0.9 5,500,164 -  Pf6N2E2_4761 0.67  -1.5 5,500,164 -  Pf6N2E2_4761 0.67  -2.4 5,500,164 -  Pf6N2E2_4761 0.67  -1.4 5,500,164 -  Pf6N2E2_4761 0.67  -1.4 5,500,164 -  Pf6N2E2_4761 0.67  -2.8 5,500,164 -  Pf6N2E2_4761 0.67  -1.1 5,500,319 -  Pf6N2E2_4761 0.80  -0.3 5,500,319 -  Pf6N2E2_4761 0.80  -2.4 5,500,319 -  Pf6N2E2_4761 0.80  -1.8 5,500,491 +  -2.2 5,500,491 +  -2.4 5,500,491 +  -3.7 5,500,491 +  +1.5 5,500,491 +  -0.6 5,500,491 +  -1.8 5,500,491 +  -1.3 5,500,492 -  -2.1 5,500,492 -  -1.1 5,500,561 +  -1.4 5,500,561 +  -0.8 5,500,561 +  -0.7 5,500,561 +  -0.7 5,500,561 +  -0.9 5,500,561 +  -0.8 5,500,562 -  -1.8 5,500,562 -  -0.9 5,500,987 +  Pf6N2E2_4762 0.60  -2.0 5,500,987 +  Pf6N2E2_4762 0.60  -1.6 5,500,988 -  Pf6N2E2_4762 0.61  -1.5 5,501,057 +  Pf6N2E2_4762 0.70  -2.9 5,501,356 +  Pf6N2E2_4763 0.11  -1.2 5,501,356 +  Pf6N2E2_4763 0.11  -0.5 5,501,356 +  Pf6N2E2_4763 0.11  -2.5 5,501,356 +  Pf6N2E2_4763 0.11  -3.3 5,501,356 +  Pf6N2E2_4763 0.11  -2.1 5,501,356 +  Pf6N2E2_4763 0.11  -2.6 5,501,356 +  Pf6N2E2_4763 0.11  -1.3 5,501,356 +  Pf6N2E2_4763 0.11  -2.0 5,501,356 +  Pf6N2E2_4763 0.11  -3.1 5,501,356 +  Pf6N2E2_4763 0.11  -2.2 5,501,356 +  Pf6N2E2_4763 0.11  -2.9 5,501,356 +  Pf6N2E2_4763 0.11  -2.2 5,501,356 +  Pf6N2E2_4763 0.11  -2.4 5,501,356 +  Pf6N2E2_4763 0.11  -1.7 5,501,356 +  Pf6N2E2_4763 0.11  -2.7 5,501,357 -  Pf6N2E2_4763 0.11  -2.1 5,501,357 -  Pf6N2E2_4763 0.11  -3.6 5,501,357 -  Pf6N2E2_4763 0.11  -2.7 5,501,357 -  Pf6N2E2_4763 0.11  -2.3 5,501,464 +  Pf6N2E2_4763 0.24  -3.5 5,501,465 -  Pf6N2E2_4763 0.24  -1.3 5,501,465 -  Pf6N2E2_4763 0.24  -1.4 5,501,735 +  Pf6N2E2_4763 0.57  -1.2 5,501,735 +  Pf6N2E2_4763 0.57  -2.7 5,501,736 -  Pf6N2E2_4763 0.57  -0.7 5,501,818 +  Pf6N2E2_4763 0.68  -2.9 5,501,818 +  Pf6N2E2_4763 0.68  -2.9 5,501,818 +  Pf6N2E2_4763 0.68  -2.9 5,501,818 +  Pf6N2E2_4763 0.68  -1.5 5,501,818 +  Pf6N2E2_4763 0.68  -3.3 5,501,819 -  Pf6N2E2_4763 0.68  +0.3 5,501,819 -  Pf6N2E2_4763 0.68  -1.3 5,501,819 -  Pf6N2E2_4763 0.68  -1.6 5,501,819 -  Pf6N2E2_4763 0.68  -0.9 5,501,819 -  Pf6N2E2_4763 0.68  -1.0 5,501,888 -  Pf6N2E2_4763 0.76  -1.7 5,502,004 +  -1.9 5,502,004 +  -0.9 5,502,004 +  -0.9 5,502,004 +  -1.9 5,502,005 -  -2.5 5,502,005 -  -0.0 5,502,043 +  -1.0 5,502,043 +  -1.5 5,502,043 +  -1.2 5,502,043 +  +0.5 5,502,043 +  -1.0 5,502,044 -  -2.1 5,502,044 -  -2.3 5,502,078 -  -2.0 5,502,090 +  -0.5 5,502,091 -  -0.5 
Or see this region's nucleotide sequence